Restriction map for
pFN21AE4237
(Linear) (Six-base) MAPSORT of: KIEE4237.seq Check: 8856 from: 1 to: 3141
KIEE4237.orf
With 182 enzymes: SgfI *
December 5, 2014 12:57 ..
AccI GT'mk_AC
Cuts at: 0 3118 3141
Size: 3118 23
AclI AA'CG_TT
Cuts at: 0 1464 3141
Size: 1464 1677
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2570 3141
Size: 2570 571
AflII C'TTAA_G
Cuts at: 0 1309 3141
Size: 1309 1832
AflIII A'CryG_T
Cuts at: 0 2226 3141
Size: 2226 915
AhdI GACnn_n'nnGTC
Cuts at: 0 1103 3141
Size: 1103 2038
AleI CACnn'nnGTG
Cuts at: 0 1643 3141
Size: 1643 1498
AlwNI CAG_nnn'CTG
Cuts at: 0 886 3141
Size: 886 2255
ApaLI G'TGCA_C
Cuts at: 0 3050 3141
Size: 3050 91
ApoI r'AATT_y
Cuts at: 0 963 1473 2173 2833 3141
Size: 963 510 700 660 308
Fragments arranged by size:
963 700 660 510 308
AseI AT'TA_AT
Cuts at: 0 1555 3141
Size: 1555 1586
AvaI C'yCGr_G
Cuts at: 0 382 448 3141
Size: 382 66 2693
Fragments arranged by size:
2693 382 66
AvrII C'CTAG_G
Cuts at: 0 213 3141
Size: 213 2928
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 860 893 3141
Size: 860 33 2248
Fragments arranged by size:
2248 860 33
BanII G_rGCy'C
Cuts at: 0 79 2852 3141
Size: 79 2773 289
Fragments arranged by size:
2773 289 79
BbsI GAAGACnn'nnnn_
Cuts at: 0 735 1004 3141
Size: 735 269 2137
Fragments arranged by size:
2137 735 269
BclI T'GATC_A
Cuts at: 0 2900 3141
Size: 2900 241
BglII A'GATC_T
Cuts at: 0 1560 3141
Size: 1560 1581
BlpI GC'TnA_GC
Cuts at: 0 2074 3141
Size: 2074 1067
Bme1580I G_kGCm'C
Cuts at: 0 240 3054 3141
Size: 240 2814 87
Fragments arranged by size:
2814 240 87
BmrI ACTGGGnnnn_n'
Cuts at: 0 250 805 1813 1900 3141
Size: 250 555 1008 87 1241
Fragments arranged by size:
1241 1008 555 250 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 74 1667 3141
Size: 63 11 1593 1474
Fragments arranged by size:
1593 1474 63 11
Bpu10I CC'TnA_GC
Cuts at: 0 2843 3141
Size: 2843 298
BsaI GGTCTCn'nnnn_
Cuts at: 0 154 3141
Size: 154 2987
BsaAI yAC'GTr
Cuts at: 0 3063 3141
Size: 3063 78
BsaBI GATnn'nnATC
Cuts at: 0 1274 3141
Size: 1274 1867
BsaWI w'CCGG_w
Cuts at: 0 163 1169 2940 3141
Size: 163 1006 1771 201
Fragments arranged by size:
1771 1006 201 163
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 510 540 3141
Size: 510 30 2601
Fragments arranged by size:
2601 510 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 71 508 515 3141
Size: 40 31 437 7 2626
Fragments arranged by size:
2626 437 40 31 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2830 3141
Size: 2830 311
BsiEI CG_ry'CG
Cuts at: 0 11 3141
Size: 11 3130
BsiHKAI G_wGCw'C
Cuts at: 0 2130 3054 3141
Size: 2130 924 87
Fragments arranged by size:
2130 924 87
BsmI GAATG_Cn'
Cuts at: 0 2955 3141
Size: 2955 186
Bsp1286I G_dGCh'C
Cuts at: 0 79 240 2130 2852 3054 3141
Size: 79 161 1890 722 202 87
Fragments arranged by size:
1890 722 202 161 87 79
BspEI T'CCGG_A
Cuts at: 0 1169 2940 3141
Size: 1169 1771 201
Fragments arranged by size:
1771 1169 201
BspHI T'CATG_A
Cuts at: 0 1590 3141
Size: 1590 1551
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 434 3141
Size: 434 2707
BsrBI CCG'CTC
Cuts at: 0 523 3141
Size: 523 2618
BsrDI GCAATG_nn'
Cuts at: 0 2989 3141
Size: 2989 152
BsrFI r'CCGG_y
Cuts at: 0 16 307 1238 3141
Size: 16 291 931 1903
Fragments arranged by size:
1903 931 291 16
BstBI TT'CG_AA
Cuts at: 0 3027 3141
Size: 3027 114
BstYI r'GATC_y
Cuts at: 0 1029 1560 1998 3141
Size: 1029 531 438 1143
Fragments arranged by size:
1143 1029 531 438
BstZ17I GTA'TAC
Cuts at: 0 3119 3141
Size: 3119 22
Bsu36I CC'TnA_GG
Cuts at: 0 583 3141
Size: 583 2558
BtgI C'CryG_G
Cuts at: 0 116 1056 2734 3141
Size: 116 940 1678 407
Fragments arranged by size:
1678 940 407 116
BtsI GCAGTG_nn'
Cuts at: 0 27 101 2411 3141
Size: 27 74 2310 730
Fragments arranged by size:
2310 730 74 27
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2358 2393 2588 2623 2810 2845 3141
Size: 2358 35 195 35 187 35 296
Fragments arranged by size:
2358 296 195 187 35 35 35
DraI TTT'AAA
Cuts at: 0 1197 2178 2456 3141
Size: 1197 981 278 685
Fragments arranged by size:
1197 981 685 278
DrdI GACnn_nn'nnGTC
Cuts at: 0 2714 3141
Size: 2714 427
EaeI y'GGCC_r
Cuts at: 0 8 305 506 2255 3141
Size: 8 297 201 1749 886
Fragments arranged by size:
1749 886 297 201 8
EagI C'GGCC_G
Cuts at: 0 8 3141
Size: 8 3133
EarI CTCTTCn'nnn_
Cuts at: 0 494 1004 1913 2156 3141
Size: 494 510 909 243 985
Fragments arranged by size:
985 909 510 494 243
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 203 3141
Size: 203 2938
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 74 1667 2570 3141
Size: 63 11 1593 903 571
Fragments arranged by size:
1593 903 571 63 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 586 1771 3141
Size: 586 1185 1370
Fragments arranged by size:
1370 1185 586
EcoO109I rG'GnC_Cy
Cuts at: 0 641 1130 2111 3141
Size: 641 489 981 1030
Fragments arranged by size:
1030 981 641 489
EcoRV GAT'ATC
Cuts at: 0 2007 3141
Size: 2007 1134
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1425 1456 3141
Size: 1425 31 1685
Fragments arranged by size:
1685 1425 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 510 542 1089 1121 1695 1727 2749 2781
Size: 510 32 547 32 574 32 1022 32
Cuts at: 2781 3141
Size: 360
Fragments arranged by size:
1022 574 547 510 360 32 32 32
32
HincII GTy'rAC
Cuts at: 0 518 681 3141
Size: 518 163 2460
Fragments arranged by size:
2460 518 163
HindIII A'AGCT_T
Cuts at: 0 1079 2290 3073 3141
Size: 1079 1211 783 68
Fragments arranged by size:
1211 1079 783 68
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 1231 1447 3141
Size: 553 678 216 1694
Fragments arranged by size:
1694 678 553 216
MscI TGG'CCA
Cuts at: 0 508 2257 3141
Size: 508 1749 884
Fragments arranged by size:
1749 884 508
MslI CAynn'nnrTG
Cuts at: 0 1643 2229 2231 2389 3017 3141
Size: 1643 586 2 158 628 124
Fragments arranged by size:
1643 628 586 158 124 2
MspA1I CmG'CkG
Cuts at: 0 73 118 3141
Size: 73 45 3023
Fragments arranged by size:
3023 73 45
NaeI GCC'GGC
Cuts at: 0 18 3141
Size: 18 3123
NcoI C'CATG_G
Cuts at: 0 1056 3141
Size: 1056 2085
NgoMIV G'CCGG_C
Cuts at: 0 16 3141
Size: 16 3125
NspI r_CATG'y
Cuts at: 0 2053 2230 3141
Size: 2053 177 911
Fragments arranged by size:
2053 911 177
PciI A'CATG_T
Cuts at: 0 2226 3141
Size: 2226 915
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1331 1363 3141
Size: 1331 32 1778
Fragments arranged by size:
1778 1331 32
PpuMI rG'GwC_Cy
Cuts at: 0 641 2111 3141
Size: 641 1470 1030
Fragments arranged by size:
1470 1030 641
PsiI TTA'TAA
Cuts at: 0 616 1085 1247 1415 3141
Size: 616 469 162 168 1726
Fragments arranged by size:
1726 616 469 168 162
PstI C_TGCA'G
Cuts at: 0 1117 1718 3141
Size: 1117 601 1423
Fragments arranged by size:
1423 1117 601
SacII CC_GC'GG
Cuts at: 0 119 3141
Size: 119 3022
SanDI GG'GwC_CC
Cuts at: 0 2111 3141
Size: 2111 1030
ScaI AGT'ACT
Cuts at: 0 1346 3141
Size: 1346 1795
SfcI C'TryA_G
Cuts at: 0 1113 1714 2948 3141
Size: 1113 601 1234 193
Fragments arranged by size:
1234 1113 601 193
SmlI C'TyrA_G
Cuts at: 0 1309 3141
Size: 1309 1832
SphI G_CATG'C
Cuts at: 0 2053 3141
Size: 2053 1088
SspI AAT'ATT
Cuts at: 0 1185 2576 3141
Size: 1185 1391 565
Fragments arranged by size:
1391 1185 565
StuI AGG'CCT
Cuts at: 0 986 3141
Size: 986 2155
StyI C'CwwG_G
Cuts at: 0 213 1056 3141
Size: 213 843 2085
Fragments arranged by size:
2085 843 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 172 2258 2612 3141
Size: 172 2086 354 529
Fragments arranged by size:
2086 529 354 172
TatI w'GTAC_w
Cuts at: 0 648 1344 2165 3141
Size: 648 696 821 976
Fragments arranged by size:
976 821 696 648
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1151 1183 3141
Size: 1151 32 1958
Fragments arranged by size:
1958 1151 32
XbaI T'CTAG_A
Cuts at: 0 2959 3141
Size: 2959 182
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 536 3141
Size: 536 2605
XmnI GAAnn'nnTTC
Cuts at: 0 1184 2066 2575 2594 3095 3141
Size: 1184 882 509 19 501 46
Fragments arranged by size:
1184 882 509 501 46 19
Enzymes that do cut:
AccI AclI AcuI AflII AflIII AhdI AleI AlwNI
ApaLI ApoI AseI AvaI AvrII BaeI BanII BbsI
BclI BglII BlpI Bme1580I BmrI BpmI Bpu10I BsaI
BsaAI BsaBI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BstBI BstYI BstZ17I Bsu36I BtgI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRV FalI Hin4I HincII HindIII MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PciI PpiI PpuMI
PsiI PstI SacII SanDI ScaI SfcI SmlI SphI
SspI StuI StyI TaqII TatI TstI XbaI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AgeI AlfI AloI ApaI
AscI AsiSI BamHI BanI BbeI BbvCI BcgI BciVI
BfrBI BglI BmgBI BmtI BplI BpuEI BsaHI BseYI
BsiWI BsmBI BsrGI BssHII BssSI BstAPI BstEII BstXI
BtgZI ClaI DraIII EcoICRI EcoRI FseI FspI FspAI
HaeII HpaI KasI KpnI MfeI MluI NarI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmeI PmlI PshAI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SalI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I
XhoI XmaI ZraI