Restriction map for
pFN21AE4234
(Linear) (Six-base) MAPSORT of: KIEE4234.seq Check: 7265 from: 1 to: 2562
KIEE4234.orf
With 182 enzymes: SgfI *
July 3, 2012 18:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 112 1040 2029 2335 2403 2562
Size: 109 3 928 989 306 68 159
Fragments arranged by size:
989 928 306 159 109 68 3
AflII C'TTAA_G
Cuts at: 0 1324 2562
Size: 1324 1238
AflIII A'CryG_T
Cuts at: 0 647 2157 2447 2562
Size: 647 1510 290 115
Fragments arranged by size:
1510 647 290 115
AhdI GACnn_n'nnGTC
Cuts at: 0 2304 2562
Size: 2304 258
AleI CACnn'nnGTG
Cuts at: 0 296 613 2562
Size: 296 317 1949
Fragments arranged by size:
1949 317 296
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 162 1266 2517 2562
Size: 162 1104 1251 45
Fragments arranged by size:
1251 1104 162 45
AlwNI CAG_nnn'CTG
Cuts at: 0 665 2562
Size: 665 1897
ApaLI G'TGCA_C
Cuts at: 0 394 2562
Size: 394 2168
ApoI r'AATT_y
Cuts at: 0 352 802 1259 1303 1399 1730 2053 2234
Size: 352 450 457 44 96 331 323 181
Cuts at: 2234 2258 2562
Size: 24 304
Fragments arranged by size:
457 450 352 331 323 304 181 96
44 24
AseI AT'TA_AT
Cuts at: 0 1675 2562
Size: 1675 887
AvaI C'yCGr_G
Cuts at: 0 620 2562
Size: 620 1942
BbsI GAAGACnn'nnnn_
Cuts at: 0 628 1769 2424 2562
Size: 628 1141 655 138
Fragments arranged by size:
1141 655 628 138
BciVI GTATCCnnnnn_n'
Cuts at: 0 662 2562
Size: 662 1900
BclI T'GATC_A
Cuts at: 0 834 1089 2562
Size: 834 255 1473
Fragments arranged by size:
1473 834 255
BglII A'GATC_T
Cuts at: 0 947 1455 1934 2562
Size: 947 508 479 628
Fragments arranged by size:
947 628 508 479
BlpI GC'TnA_GC
Cuts at: 0 2411 2562
Size: 2411 151
Bme1580I G_kGCm'C
Cuts at: 0 184 398 620 2007 2562
Size: 184 214 222 1387 555
Fragments arranged by size:
1387 555 222 214 184
BmrI ACTGGGnnnn_n'
Cuts at: 0 35 2562
Size: 35 2527
BmtI G_CTAG'C
Cuts at: 0 146 2562
Size: 146 2416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 1472 2562
Size: 365 1107 1090
Fragments arranged by size:
1107 1090 365
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1951 2562
Size: 1951 611
BsaI GGTCTCn'nnnn_
Cuts at: 0 1073 1805 2562
Size: 1073 732 757
Fragments arranged by size:
1073 757 732
BsaWI w'CCGG_w
Cuts at: 0 436 2562
Size: 436 2126
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 1238 2374 2562
Size: 28 1210 1136 188
Fragments arranged by size:
1210 1136 188 28
BseYI C'CCAG_C
Cuts at: 0 698 2476 2562
Size: 698 1778 86
Fragments arranged by size:
1778 698 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 2477 2562
Size: 343 2134 85
Fragments arranged by size:
2134 343 85
BsiHKAI G_wGCw'C
Cuts at: 0 293 398 724 2562
Size: 293 105 326 1838
Fragments arranged by size:
1838 326 293 105
Bsp1286I G_dGCh'C
Cuts at: 0 184 293 398 620 724 2007 2562
Size: 184 109 105 222 104 1283 555
Fragments arranged by size:
1283 555 222 184 109 105 104
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 315 2562
Size: 315 2247
BsrDI GCAATG_nn'
Cuts at: 0 1581 1908 2436 2562
Size: 1581 327 528 126
Fragments arranged by size:
1581 528 327 126
BsrFI r'CCGG_y
Cuts at: 0 2441 2562
Size: 2441 121
BsrGI T'GTAC_A
Cuts at: 0 1550 2562
Size: 1550 1012
BssSI C'ACGA_G
Cuts at: 0 358 2562
Size: 358 2204
BstAPI GCAn_nnn'nTGC
Cuts at: 0 665 2262 2510 2562
Size: 665 1597 248 52
Fragments arranged by size:
1597 665 248 52
BstXI CCAn_nnnn'nTGG
Cuts at: 0 567 2562
Size: 567 1995
BstYI r'GATC_y
Cuts at: 0 412 947 1455 1934 2204 2562
Size: 412 535 508 479 270 358
Fragments arranged by size:
535 508 479 412 358 270
BtgI C'CryG_G
Cuts at: 0 2526 2562
Size: 2526 36
BtsI GCAGTG_nn'
Cuts at: 0 2520 2562
Size: 2520 42
DraI TTT'AAA
Cuts at: 0 1308 2562
Size: 1308 1254
DraIII CAC_nnn'GTG
Cuts at: 0 1215 2562
Size: 1215 1347
EaeI y'GGCC_r
Cuts at: 0 1067 2562
Size: 1067 1495
EarI CTCTTCn'nnn_
Cuts at: 0 59 68 938 957 1955 2562
Size: 59 9 870 19 998 607
Fragments arranged by size:
998 870 607 59 19 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 2562
Size: 19 2543
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 112 365 1040 1472 2029 2335 2403
Size: 109 3 253 675 432 557 306 68
Cuts at: 2403 2562
Size: 159
Fragments arranged by size:
675 557 432 306 253 159 109 68
3
EcoO109I rG'GnC_Cy
Cuts at: 0 29 2562
Size: 29 2533
EcoRI G'AATT_C
Cuts at: 0 802 2562
Size: 802 1760
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2365 2562
Size: 2365 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 528 560 577 609 899 931 1956 1988
Size: 528 32 17 32 290 32 1025 32
Cuts at: 1988 2290 2322 2562
Size: 302 32 240
Fragments arranged by size:
1025 528 302 290 240 32 32 32
32 32 17
HincII GTy'rAC
Cuts at: 0 1704 1942 2163 2562
Size: 1704 238 221 399
Fragments arranged by size:
1704 399 238 221
HindIII A'AGCT_T
Cuts at: 0 1798 2562
Size: 1798 764
MscI TGG'CCA
Cuts at: 0 1069 2562
Size: 1069 1493
MslI CAynn'nnrTG
Cuts at: 0 296 298 613 743 1248 2562
Size: 296 2 315 130 505 1314
Fragments arranged by size:
1314 505 315 296 130 2
MspA1I CmG'CkG
Cuts at: 0 1144 2562
Size: 1144 1418
NaeI GCC'GGC
Cuts at: 0 2443 2562
Size: 2443 119
NgoMIV G'CCGG_C
Cuts at: 0 2441 2562
Size: 2441 121
NheI G'CTAG_C
Cuts at: 0 142 2562
Size: 142 2420
NspI r_CATG'y
Cuts at: 0 651 742 1710 1786 2161 2451 2562
Size: 651 91 968 76 375 290 111
Fragments arranged by size:
968 651 375 290 111 91 76
PciI A'CATG_T
Cuts at: 0 647 2157 2447 2562
Size: 647 1510 290 115
Fragments arranged by size:
1510 647 290 115
PpuMI rG'GwC_Cy
Cuts at: 0 29 2562
Size: 29 2533
PshAI GACnn'nnGTC
Cuts at: 0 709 2562
Size: 709 1853
PsiI TTA'TAA
Cuts at: 0 1301 2562
Size: 1301 1261
PstI C_TGCA'G
Cuts at: 0 661 2515 2562
Size: 661 1854 47
Fragments arranged by size:
1854 661 47
PvuII CAG'CTG
Cuts at: 0 1144 2562
Size: 1144 1418
SanDI GG'GwC_CC
Cuts at: 0 29 2562
Size: 29 2533
SexAI A'CCwGG_T
Cuts at: 0 1211 2562
Size: 1211 1351
SfcI C'TryA_G
Cuts at: 0 44 657 1777 2511 2562
Size: 44 613 1120 734 51
Fragments arranged by size:
1120 734 613 51 44
SmaI CCC'GGG
Cuts at: 0 622 2562
Size: 622 1940
SmlI C'TyrA_G
Cuts at: 0 1324 1930 2562
Size: 1324 606 632
Fragments arranged by size:
1324 632 606
SphI G_CATG'C
Cuts at: 0 742 2562
Size: 742 1820
SspI AAT'ATT
Cuts at: 0 1164 2562
Size: 1164 1398
StuI AGG'CCT
Cuts at: 0 903 2562
Size: 903 1659
StyI C'CwwG_G
Cuts at: 0 1070 2060 2225 2562
Size: 1070 990 165 337
Fragments arranged by size:
1070 990 337 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 286 413 559 2562
Size: 286 127 146 2003
Fragments arranged by size:
2003 286 146 127
TatI w'GTAC_w
Cuts at: 0 846 1550 2562
Size: 846 704 1012
Fragments arranged by size:
1012 846 704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 906 1261 2562
Size: 906 355 1301
Fragments arranged by size:
1301 906 355
XmaI C'CCGG_G
Cuts at: 0 620 2562
Size: 620 1942
XmnI GAAnn'nnTTC
Cuts at: 0 125 353 2562
Size: 125 228 2209
Fragments arranged by size:
2209 228 125
Enzymes that do cut:
AcuI AflII AflIII AhdI AleI AlfI AlwNI ApaLI
ApoI AseI AvaI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BmtI BpmI BpuEI BsaI BsaWI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BsrGI
BssSI BstAPI BstXI BstYI BtgI BtsI DraI DraIII
EaeI EarI EciI Eco57MI EcoO109I EcoRI FalI Hin4I
HincII HindIII MscI MslI MspA1I NaeI NgoMIV NheI
NspI PciI PpuMI PshAI PsiI PstI PvuII SanDI
SexAI SfcI SmaI SmlI SphI SspI StuI StyI
TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AloI
ApaI AscI AsiSI AvrII BaeI BamHI BanI BanII
BbeI BbvCI BcgI BfrBI BglI BmgBI BplI Bpu10I
BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BssHII BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DrdI EagI EcoICRI EcoNI EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MmeI NarI NcoI NdeI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SwaI TstI Tth111I XbaI XhoI ZraI