Restriction map for
pFN21AE4233
(Linear) (Six-base) MAPSORT of: KIEE4233.seq Check: 7277 from: 1 to: 2550
KIEE4233.orf
With 182 enzymes: SgfI *
March 22, 2012 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2218 2550
Size: 2218 332
AccI GT'mk_AC
Cuts at: 0 1379 2550
Size: 1379 1171
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 950 2550
Size: 950 1600
AflII C'TTAA_G
Cuts at: 0 2311 2550
Size: 2311 239
AhdI GACnn_n'nnGTC
Cuts at: 0 918 2550
Size: 918 1632
AleI CACnn'nnGTG
Cuts at: 0 1138 2550
Size: 1138 1412
AlwNI CAG_nnn'CTG
Cuts at: 0 117 2550
Size: 117 2433
ApaI G_GGCC'C
Cuts at: 0 604 2550
Size: 604 1946
ApaLI G'TGCA_C
Cuts at: 0 1810 2550
Size: 1810 740
ApoI r'AATT_y
Cuts at: 0 144 198 268 1194 1702 1763 1872 2282
Size: 144 54 70 926 508 61 109 410
Cuts at: 2282 2550
Size: 268
Fragments arranged by size:
926 508 410 268 144 109 70 61
54
AvaI C'yCGr_G
Cuts at: 0 70 2550
Size: 70 2480
AvrII C'CTAG_G
Cuts at: 0 804 2550
Size: 804 1746
BanI G'GyrC_C
Cuts at: 0 12 42 2550
Size: 12 30 2508
Fragments arranged by size:
2508 30 12
BanII G_rGCy'C
Cuts at: 0 71 604 1845 1913 2002 2550
Size: 71 533 1241 68 89 548
Fragments arranged by size:
1241 548 533 89 71 68
BbeI G_GCGC'C
Cuts at: 0 16 46 2550
Size: 16 30 2504
Fragments arranged by size:
2504 30 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 383 631 956 2270 2550
Size: 383 248 325 1314 280
Fragments arranged by size:
1314 383 325 280 248
BbvCI CC'TCA_GC
Cuts at: 0 1032 2550
Size: 1032 1518
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1874 1908 2550
Size: 1874 34 642
Fragments arranged by size:
1874 642 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1155 2550
Size: 1155 1395
BglI GCCn_nnn'nGGC
Cuts at: 0 122 2550
Size: 122 2428
BglII A'GATC_T
Cuts at: 0 128 342 2550
Size: 128 214 2208
Fragments arranged by size:
2208 214 128
Bme1580I G_kGCm'C
Cuts at: 0 604 1247 1814 2550
Size: 604 643 567 736
Fragments arranged by size:
736 643 604 567
BmrI ACTGGGnnnn_n'
Cuts at: 0 711 1954 2550
Size: 711 1243 596
Fragments arranged by size:
1243 711 596
Bpu10I CC'TnA_GC
Cuts at: 0 1032 2550
Size: 1032 1518
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1829 2550
Size: 1829 721
BsaI GGTCTCn'nnnn_
Cuts at: 0 926 2550
Size: 926 1624
BsaAI yAC'GTr
Cuts at: 0 957 2550
Size: 957 1593
BsaHI Gr'CG_yC
Cuts at: 0 13 43 2550
Size: 13 30 2507
Fragments arranged by size:
2507 30 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1328 1358 2550
Size: 1328 30 1192
Fragments arranged by size:
1328 1192 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 748 1284 2550
Size: 748 536 1266
Fragments arranged by size:
1266 748 536
BseYI C'CCAG_C
Cuts at: 0 1649 2550
Size: 1649 901
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1090 2550
Size: 1090 1460
BsiHKAI G_wGCw'C
Cuts at: 0 1814 1845 1913 2002 2550
Size: 1814 31 68 89 548
Fragments arranged by size:
1814 548 89 68 31
Bsp1286I G_dGCh'C
Cuts at: 0 71 604 1247 1814 1845 1913 2002 2550
Size: 71 533 643 567 31 68 89 548
Fragments arranged by size:
643 567 548 533 89 71 68 31
BspHI T'CATG_A
Cuts at: 0 2472 2550
Size: 2472 78
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2218 2550
Size: 2218 332
BsrGI T'GTAC_A
Cuts at: 0 522 2550
Size: 522 2028
BstAPI GCAn_nnn'nTGC
Cuts at: 0 117 2550
Size: 117 2433
BstEII G'GTnAC_C
Cuts at: 0 684 2550
Size: 684 1866
BstXI CCAn_nnnn'nTGG
Cuts at: 0 201 1140 2550
Size: 201 939 1410
Fragments arranged by size:
1410 939 201
BstYI r'GATC_y
Cuts at: 0 128 342 691 2494 2550
Size: 128 214 349 1803 56
Fragments arranged by size:
1803 349 214 128 56
BtgI C'CryG_G
Cuts at: 0 540 2550
Size: 540 2010
BtsI GCAGTG_nn'
Cuts at: 0 1133 1514 2550
Size: 1133 381 1036
Fragments arranged by size:
1133 1036 381
ClaI AT'CG_AT
Cuts at: 0 1313 2550
Size: 1313 1237
EaeI y'GGCC_r
Cuts at: 0 561 2550
Size: 561 1989
EarI CTCTTCn'nnn_
Cuts at: 0 725 929 1988 2324 2550
Size: 725 204 1059 336 226
Fragments arranged by size:
1059 725 336 226 204
EcoICRI GAG'CTC
Cuts at: 0 1843 1911 2000 2550
Size: 1843 68 89 550
Fragments arranged by size:
1843 550 89 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 950 2550
Size: 950 1600
EcoO109I rG'GnC_Cy
Cuts at: 0 601 2550
Size: 601 1949
EcoRV GAT'ATC
Cuts at: 0 1440 2550
Size: 1440 1110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 478 2550
Size: 478 2072
HaeII r_GCGC'y
Cuts at: 0 16 46 2550
Size: 16 30 2504
Fragments arranged by size:
2504 30 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 904 936 1452 1484 2550
Size: 904 32 516 32 1066
Fragments arranged by size:
1066 904 516 32 32
HincII GTy'rAC
Cuts at: 0 429 2550
Size: 429 2121
KasI G'GCGC_C
Cuts at: 0 12 42 2550
Size: 12 30 2508
Fragments arranged by size:
2508 30 12
MfeI C'AATT_G
Cuts at: 0 1397 2531 2550
Size: 1397 1134 19
Fragments arranged by size:
1397 1134 19
MscI TGG'CCA
Cuts at: 0 563 2550
Size: 563 1987
MslI CAynn'nnrTG
Cuts at: 0 1138 1429 1589 1695 2550
Size: 1138 291 160 106 855
Fragments arranged by size:
1138 855 291 160 106
MspA1I CmG'CkG
Cuts at: 0 49 1036 2550
Size: 49 987 1514
Fragments arranged by size:
1514 987 49
NarI GG'CG_CC
Cuts at: 0 13 43 2550
Size: 13 30 2507
Fragments arranged by size:
2507 30 13
NcoI C'CATG_G
Cuts at: 0 540 2550
Size: 540 2010
NruI TCG'CGA
Cuts at: 0 95 2550
Size: 95 2455
NspI r_CATG'y
Cuts at: 0 539 2550
Size: 539 2011
PflMI CCAn_nnn'nTGG
Cuts at: 0 160 234 2536 2550
Size: 160 74 2302 14
Fragments arranged by size:
2302 160 74 14
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1599 1631 2550
Size: 1599 32 919
Fragments arranged by size:
1599 919 32
PspOMI G'GGCC_C
Cuts at: 0 600 2550
Size: 600 1950
PstI C_TGCA'G
Cuts at: 0 66 1509 2107 2229 2241 2321 2550
Size: 66 1443 598 122 12 80 229
Fragments arranged by size:
1443 598 229 122 80 66 12
PvuII CAG'CTG
Cuts at: 0 1036 2550
Size: 1036 1514
SacI G_AGCT'C
Cuts at: 0 1845 1913 2002 2550
Size: 1845 68 89 548
Fragments arranged by size:
1845 548 89 68
ScaI AGT'ACT
Cuts at: 0 973 2550
Size: 973 1577
SfcI C'TryA_G
Cuts at: 0 62 871 1505 1541 2103 2225 2237 2317
Size: 62 809 634 36 562 122 12 80
Cuts at: 2317 2550
Size: 233
Fragments arranged by size:
809 634 562 233 122 80 62 36
12
SfoI GGC'GCC
Cuts at: 0 14 44 2550
Size: 14 30 2506
Fragments arranged by size:
2506 30 14
SmlI C'TyrA_G
Cuts at: 0 1844 2311 2550
Size: 1844 467 239
Fragments arranged by size:
1844 467 239
SphI G_CATG'C
Cuts at: 0 539 2550
Size: 539 2011
StyI C'CwwG_G
Cuts at: 0 540 804 2550
Size: 540 264 1746
Fragments arranged by size:
1746 540 264
TatI w'GTAC_w
Cuts at: 0 522 971 2221 2550
Size: 522 449 1250 329
Fragments arranged by size:
1250 522 449 329
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2039 2071 2550
Size: 2039 32 479
Fragments arranged by size:
2039 479 32
XmnI GAAnn'nnTTC
Cuts at: 0 843 2286 2550
Size: 843 1443 264
Fragments arranged by size:
1443 843 264
Enzymes that do cut:
AarI AccI AcuI AflII AhdI AleI AlwNI ApaI
ApaLI ApoI AvaI AvrII BanI BanII BbeI BbsI
BbvCI BcgI BciVI BglI BglII Bme1580I BmrI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrGI BstAPI BstEII BstXI
BstYI BtgI BtsI ClaI EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRV FalI HaeII Hin4I HincII KasI MfeI
MscI MslI MspA1I NarI NcoI NruI NspI PflMI
PpiI PspOMI PstI PvuII SacI ScaI SfcI SfoI
SmlI SphI StyI TatI TstI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AlfI AloI
AscI AseI AsiSI BaeI BamHI BclI BfrBI BlpI
BmgBI BmtI BplI BpmI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BsrBI BsrDI BsrFI BssHII BssSI
BstBI BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI
EagI EciI EcoNI EcoRI FseI FspI FspAI HindIII
HpaI KpnI MluI MmeI NaeI NdeI NgoMIV NheI
NotI NsiI PacI PasI PciI PfoI PmeI PmlI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI ZraI