Restriction map for
pFN21AE4232
(Linear) (Six-base) MAPSORT of: KIEE4232.seq Check: 9393 from: 1 to: 2475
KIEE4232.orf
With 182 enzymes: SgfI *
March 22, 2012 17:37 ..
Acc65I G'GTAC_C
Cuts at: 0 461 2475
Size: 461 2014
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 297 421 1527 1730 1930 2043 2071
Size: 183 114 124 1106 203 200 113 28
Cuts at: 2071 2475
Size: 404
Fragments arranged by size:
1106 404 203 200 183 124 114 113
28
AflIII A'CryG_T
Cuts at: 0 2092 2183 2475
Size: 2092 91 292
Fragments arranged by size:
2092 292 91
AhdI GACnn_n'nnGTC
Cuts at: 0 537 2475
Size: 537 1938
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2086 2475
Size: 2086 389
ApaLI G'TGCA_C
Cuts at: 0 2375 2475
Size: 2375 100
ApoI r'AATT_y
Cuts at: 0 13 903 2341 2407 2475
Size: 13 890 1438 66 68
Fragments arranged by size:
1438 890 68 66 13
AvaI C'yCGr_G
Cuts at: 0 391 818 893 1056 2475
Size: 391 427 75 163 1419
Fragments arranged by size:
1419 427 391 163 75
BamHI G'GATC_C
Cuts at: 0 318 2288 2475
Size: 318 1970 187
Fragments arranged by size:
1970 318 187
BanI G'GyrC_C
Cuts at: 0 145 211 461 736 768 779 799 1227
Size: 145 66 250 275 32 11 20 428
Cuts at: 1227 1392 2009 2475
Size: 165 617 466
Fragments arranged by size:
617 466 428 275 250 165 145 66
32 20 11
BanII G_rGCy'C
Cuts at: 0 280 1126 1741 2475
Size: 280 846 615 734
Fragments arranged by size:
846 734 615 280
BbeI G_GCGC'C
Cuts at: 0 740 1396 2475
Size: 740 656 1079
Fragments arranged by size:
1079 740 656
BbvCI CC'TCA_GC
Cuts at: 0 1174 2475
Size: 1174 1301
BciVI GTATCCnnnnn_n'
Cuts at: 0 1688 2475
Size: 1688 787
BlpI GC'TnA_GC
Cuts at: 0 104 587 865 2475
Size: 104 483 278 1610
Fragments arranged by size:
1610 483 278 104
Bme1580I G_kGCm'C
Cuts at: 0 214 1201 1350 1855 2379 2475
Size: 214 987 149 505 524 96
Fragments arranged by size:
987 524 505 214 149 96
BmgBI CAC'GTC
Cuts at: 0 1994 2475
Size: 1994 481
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1551 2475
Size: 1551 924
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 805 1546 2475
Size: 433 372 741 929
Fragments arranged by size:
929 741 433 372
Bpu10I CC'TnA_GC
Cuts at: 0 430 1120 1174 1202 1214 1740 2475
Size: 430 690 54 28 12 526 735
Fragments arranged by size:
735 690 526 430 54 28 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 2475
Size: 1222 1253
BsaI GGTCTCn'nnnn_
Cuts at: 0 1149 2475
Size: 1149 1326
BsaAI yAC'GTr
Cuts at: 0 702 1665 2475
Size: 702 963 810
Fragments arranged by size:
963 810 702
BsaBI GATnn'nnATC
Cuts at: 0 122 323 2475
Size: 122 201 2152
Fragments arranged by size:
2152 201 122
BsaHI Gr'CG_yC
Cuts at: 0 737 884 1154 1393 1415 1516 2475
Size: 737 147 270 239 22 101 959
Fragments arranged by size:
959 737 270 239 147 101 22
BsaWI w'CCGG_w
Cuts at: 0 605 2270 2475
Size: 605 1665 205
Fragments arranged by size:
1665 605 205
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 164 430 433 802 913 1137 1527 1734
Size: 164 266 3 369 111 224 390 207
Cuts at: 1734 2441 2475
Size: 707 34
Fragments arranged by size:
707 390 369 266 224 207 164 111
34 3
BseYI C'CCAG_C
Cuts at: 0 725 1125 1162 1299 1658 2475
Size: 725 400 37 137 359 817
Fragments arranged by size:
817 725 400 359 137 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 664 2475
Size: 523 141 1811
Fragments arranged by size:
1811 523 141
BsiEI CG_ry'CG
Cuts at: 0 471 2475
Size: 471 2004
BsiHKAI G_wGCw'C
Cuts at: 0 1741 2379 2475
Size: 1741 638 96
Fragments arranged by size:
1741 638 96
BsmI GAATG_Cn'
Cuts at: 0 2283 2475
Size: 2283 192
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1256 2475
Size: 1256 1219
Bsp1286I G_dGCh'C
Cuts at: 0 214 280 1126 1201 1350 1741 1855 2379
Size: 214 66 846 75 149 391 114 524
Cuts at: 2379 2475
Size: 96
Fragments arranged by size:
846 524 391 214 149 114 96 75
66
BspHI T'CATG_A
Cuts at: 0 1949 2475
Size: 1949 526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 80 452 1031 1473 2287 2475
Size: 80 372 579 442 814 188
Fragments arranged by size:
814 579 442 372 188 80
BsrBI CCG'CTC
Cuts at: 0 1870 2475
Size: 1870 605
BsrDI GCAATG_nn'
Cuts at: 0 1610 1823 2475
Size: 1610 213 652
Fragments arranged by size:
1610 652 213
BsrFI r'CCGG_y
Cuts at: 0 39 290 713 2475
Size: 39 251 423 1762
Fragments arranged by size:
1762 423 251 39
BstAPI GCAn_nnn'nTGC
Cuts at: 0 906 2475
Size: 906 1569
BstXI CCAn_nnnn'nTGG
Cuts at: 0 733 2475
Size: 733 1742
BstYI r'GATC_y
Cuts at: 0 318 2288 2475
Size: 318 1970 187
Fragments arranged by size:
1970 318 187
BtgI C'CryG_G
Cuts at: 0 71 154 235 280 465 875 1919 2475
Size: 71 83 81 45 185 410 1044 556
Fragments arranged by size:
1044 556 410 185 83 81 71 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 94 1638 2475
Size: 94 1544 837
Fragments arranged by size:
1544 837 94
BtsI GCAGTG_nn'
Cuts at: 0 1136 1498 1835 2122 2475
Size: 1136 362 337 287 353
Fragments arranged by size:
1136 362 353 337 287
DrdI GACnn_nn'nnGTC
Cuts at: 0 1648 2475
Size: 1648 827
EaeI y'GGCC_r
Cuts at: 0 468 854 1814 1871 2249 2475
Size: 468 386 960 57 378 226
Fragments arranged by size:
960 468 386 378 226 57
EagI C'GGCC_G
Cuts at: 0 468 2475
Size: 468 2007
EarI CTCTTCn'nnn_
Cuts at: 0 978 1706 2475
Size: 978 728 769
Fragments arranged by size:
978 769 728
EcoICRI GAG'CTC
Cuts at: 0 1739 2475
Size: 1739 736
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 297 421 433 805 1527 1546 1730
Size: 183 114 124 12 372 722 19 184
Cuts at: 1730 1930 2043 2071 2475
Size: 200 113 28 404
Fragments arranged by size:
722 404 372 200 184 183 124 114
113 28 19 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 835 1542 2475
Size: 835 707 933
Fragments arranged by size:
933 835 707
EcoO109I rG'GnC_Cy
Cuts at: 0 92 169 185 386 441 1399 1926 2475
Size: 92 77 16 201 55 958 527 549
Fragments arranged by size:
958 549 527 201 92 77 55 16
FspI TGC'GCA
Cuts at: 0 448 2475
Size: 448 2027
HaeII r_GCGC'y
Cuts at: 0 740 1174 1396 2157 2475
Size: 740 434 222 761 318
Fragments arranged by size:
761 740 434 318 222
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 523 555 756 788 1546 1578 2475
Size: 523 32 201 32 758 32 897
Fragments arranged by size:
897 758 523 201 32 32 32
HincII GTy'rAC
Cuts at: 0 1192 1419 2475
Size: 1192 227 1056
Fragments arranged by size:
1192 1056 227
KasI G'GCGC_C
Cuts at: 0 736 1392 2475
Size: 736 656 1083
Fragments arranged by size:
1083 736 656
KpnI G_GTAC'C
Cuts at: 0 465 2475
Size: 465 2010
MscI TGG'CCA
Cuts at: 0 856 1816 2475
Size: 856 960 659
Fragments arranged by size:
960 856 659
MslI CAynn'nnrTG
Cuts at: 0 40 1732 1828 1894 2475
Size: 40 1692 96 66 581
Fragments arranged by size:
1692 581 96 66 40
MspA1I CmG'CkG
Cuts at: 0 1779 2049 2475
Size: 1779 270 426
Fragments arranged by size:
1779 426 270
NaeI GCC'GGC
Cuts at: 0 715 2475
Size: 715 1760
NarI GG'CG_CC
Cuts at: 0 737 1393 2475
Size: 737 656 1082
Fragments arranged by size:
1082 737 656
NcoI C'CATG_G
Cuts at: 0 71 280 875 1919 2475
Size: 71 209 595 1044 556
Fragments arranged by size:
1044 595 556 209 71
NgoMIV G'CCGG_C
Cuts at: 0 713 2475
Size: 713 1762
NspI r_CATG'y
Cuts at: 0 39 975 2096 2187 2475
Size: 39 936 1121 91 288
Fragments arranged by size:
1121 936 288 91 39
PasI CC'CwG_GG
Cuts at: 0 547 911 1350 2475
Size: 547 364 439 1125
Fragments arranged by size:
1125 547 439 364
PciI A'CATG_T
Cuts at: 0 2092 2183 2475
Size: 2092 91 292
Fragments arranged by size:
2092 292 91
PfoI T'CCnGG_A
Cuts at: 0 269 1523 2475
Size: 269 1254 952
Fragments arranged by size:
1254 952 269
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1552 1584 2475
Size: 1552 32 891
Fragments arranged by size:
1552 891 32
PpuMI rG'GwC_Cy
Cuts at: 0 92 169 185 386 441 1399 1926 2475
Size: 92 77 16 201 55 958 527 549
Fragments arranged by size:
958 549 527 201 92 77 55 16
PshAI GACnn'nnGTC
Cuts at: 0 200 486 2475
Size: 200 286 1989
Fragments arranged by size:
1989 286 200
PstI C_TGCA'G
Cuts at: 0 9 2262 2475
Size: 9 2253 213
Fragments arranged by size:
2253 213 9
PvuII CAG'CTG
Cuts at: 0 1779 2049 2475
Size: 1779 270 426
Fragments arranged by size:
1779 426 270
SacI G_AGCT'C
Cuts at: 0 1741 2475
Size: 1741 734
SapI GCTCTTCn'nnn_
Cuts at: 0 978 2475
Size: 978 1497
SfcI C'TryA_G
Cuts at: 0 5 633 2258 2475
Size: 5 628 1625 217
Fragments arranged by size:
1625 628 217 5
SfoI GGC'GCC
Cuts at: 0 738 1394 2475
Size: 738 656 1081
Fragments arranged by size:
1081 738 656
SmaI CCC'GGG
Cuts at: 0 820 2475
Size: 820 1655
SmlI C'TyrA_G
Cuts at: 0 1237 2475
Size: 1237 1238
StuI AGG'CCT
Cuts at: 0 631 1745 2475
Size: 631 1114 730
Fragments arranged by size:
1114 730 631
StyI C'CwwG_G
Cuts at: 0 71 280 362 836 875 1456 1646 1919
Size: 71 209 82 474 39 581 190 273
Cuts at: 1919 2475
Size: 556
Fragments arranged by size:
581 556 474 273 209 190 82 71
39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 868 1880 2035 2475
Size: 868 1012 155 440
Fragments arranged by size:
1012 868 440 155
TatI w'GTAC_w
Cuts at: 0 2015 2475
Size: 2015 460
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 214 246 2082 2114 2475
Size: 214 32 1836 32 361
Fragments arranged by size:
1836 361 214 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 684 2475
Size: 684 1791
XmaI C'CCGG_G
Cuts at: 0 818 2475
Size: 818 1657
XmnI GAAnn'nnTTC
Cuts at: 0 2056 2475
Size: 2056 419
Enzymes that do cut:
Acc65I AcuI AflIII AhdI AlfI ApaLI ApoI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BlpI Bme1580I
BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI
BstXI BstYI BtgI BtgZI BtsI DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
HincII KasI KpnI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PpiI PpuMI
PshAI PstI PvuII SacI SapI SfcI SfoI SmaI
SmlI StuI StyI TaqII TatI TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AleI
AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI
BbsI BcgI BclI BfrBI BglI BglII BmrI BmtI
BsaXI BsiWI BspEI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI DraIII EciI EcoRI
EcoRV FalI FseI FspAI HindIII HpaI MfeI MluI
MmeI NdeI NheI NotI NruI NsiI PacI PflMI
PmeI PmlI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI ZraI