Restriction map for
pFN21AE4231
(Linear) (Six-base) MAPSORT of: KIEE4231.seq Check: 3418 from: 1 to: 2445
KIEE4231.orf
With 182 enzymes: SgfI *
March 22, 2012 17:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 1873 1879 2105 2216 2408 2445
Size: 623 1250 6 226 111 192 37
Fragments arranged by size:
1250 623 226 192 111 37 6
AflII C'TTAA_G
Cuts at: 0 252 2445
Size: 252 2193
AhdI GACnn_n'nnGTC
Cuts at: 0 1430 2445
Size: 1430 1015
ApoI r'AATT_y
Cuts at: 0 152 225 292 456 1878 2445
Size: 152 73 67 164 1422 567
Fragments arranged by size:
1422 567 164 152 73 67
AseI AT'TA_AT
Cuts at: 0 269 2021 2445
Size: 269 1752 424
Fragments arranged by size:
1752 424 269
AvaI C'yCGr_G
Cuts at: 0 476 2445
Size: 476 1969
BamHI G'GATC_C
Cuts at: 0 546 1627 2445
Size: 546 1081 818
Fragments arranged by size:
1081 818 546
BanII G_rGCy'C
Cuts at: 0 667 1180 1645 2445
Size: 667 513 465 800
Fragments arranged by size:
800 667 513 465
BbvCI CC'TCA_GC
Cuts at: 0 1232 2445
Size: 1232 1213
BglII A'GATC_T
Cuts at: 0 430 2445
Size: 430 2015
Bme1580I G_kGCm'C
Cuts at: 0 14 2445
Size: 14 2431
BmgBI CAC'GTC
Cuts at: 0 1337 2445
Size: 1337 1108
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 369 1826 2147 2445
Size: 369 1457 321 298
Fragments arranged by size:
1457 369 321 298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1202 1438 2156 2445
Size: 1202 236 718 289
Fragments arranged by size:
1202 718 289 236
Bpu10I CC'TnA_GC
Cuts at: 0 1232 2445
Size: 1232 1213
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 1629 2445
Size: 78 1551 816
Fragments arranged by size:
1551 816 78
BsaBI GATnn'nnATC
Cuts at: 0 197 1055 1159 1322 2445
Size: 197 858 104 163 1123
Fragments arranged by size:
1123 858 197 163 104
BsaHI Gr'CG_yC
Cuts at: 0 2320 2445
Size: 2320 125
BsaWI w'CCGG_w
Cuts at: 0 1331 2445
Size: 1331 1114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2382 2412 2445
Size: 2382 30 33
Fragments arranged by size:
2382 33 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1435 2390 2445
Size: 1435 955 55
Fragments arranged by size:
1435 955 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1334 2445
Size: 1334 1111
BsiEI CG_ry'CG
Cuts at: 0 41 2445
Size: 41 2404
BsiHKAI G_wGCw'C
Cuts at: 0 1180 1807 2445
Size: 1180 627 638
Fragments arranged by size:
1180 638 627
BsmI GAATG_Cn'
Cuts at: 0 915 2445
Size: 915 1530
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1475 2445
Size: 1475 970
Bsp1286I G_dGCh'C
Cuts at: 0 14 667 1180 1645 1807 2445
Size: 14 653 513 465 162 638
Fragments arranged by size:
653 638 513 465 162 14
BspEI T'CCGG_A
Cuts at: 0 1331 2445
Size: 1331 1114
BspHI T'CATG_A
Cuts at: 0 1080 2445
Size: 1080 1365
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 921 2445
Size: 921 1524
BsrFI r'CCGG_y
Cuts at: 0 40 2445
Size: 40 2405
BsrGI T'GTAC_A
Cuts at: 0 1200 2445
Size: 1200 1245
BssSI C'ACGA_G
Cuts at: 0 879 2445
Size: 879 1566
BstAPI GCAn_nnn'nTGC
Cuts at: 0 211 2445
Size: 211 2234
BstEII G'GTnAC_C
Cuts at: 0 862 2445
Size: 862 1583
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1638 1681 1716 2445
Size: 1638 43 35 729
Fragments arranged by size:
1638 729 43 35
BstYI r'GATC_y
Cuts at: 0 198 430 546 1571 1627 1701 2445
Size: 198 232 116 1025 56 74 744
Fragments arranged by size:
1025 744 232 198 116 74 56
BtgI C'CryG_G
Cuts at: 0 29 2445
Size: 29 2416
BtsI GCAGTG_nn'
Cuts at: 0 12 369 965 1709 2445
Size: 12 357 596 744 736
Fragments arranged by size:
744 736 596 357 12
DraI TTT'AAA
Cuts at: 0 224 701 2445
Size: 224 477 1744
Fragments arranged by size:
1744 477 224
DrdI GACnn_nn'nnGTC
Cuts at: 0 1221 2445
Size: 1221 1224
EaeI y'GGCC_r
Cuts at: 0 38 573 875 2445
Size: 38 535 302 1570
Fragments arranged by size:
1570 535 302 38
EagI C'GGCC_G
Cuts at: 0 38 2445
Size: 38 2407
EarI CTCTTCn'nnn_
Cuts at: 0 856 1763 2445
Size: 856 907 682
Fragments arranged by size:
907 856 682
EcoICRI GAG'CTC
Cuts at: 0 1178 2445
Size: 1178 1267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 1202 1438 1873 1879 2105 2156 2216
Size: 623 579 236 435 6 226 51 60
Cuts at: 2216 2408 2445
Size: 192 37
Fragments arranged by size:
623 579 435 236 226 192 60 51
37 6
EcoO109I rG'GnC_Cy
Cuts at: 0 1228 1465 2445
Size: 1228 237 980
Fragments arranged by size:
1228 980 237
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1053 2438 2445
Size: 1053 1385 7
Fragments arranged by size:
1385 1053 7
HaeII r_GCGC'y
Cuts at: 0 1659 2445
Size: 1659 786
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1349 1356 1381 1388 1416 1448 2113 2145
Size: 1349 7 25 7 28 32 665 32
Cuts at: 2145 2445
Size: 300
Fragments arranged by size:
1349 665 300 32 32 28 25 7
7
HincII GTy'rAC
Cuts at: 0 1584 1728 2445
Size: 1584 144 717
Fragments arranged by size:
1584 717 144
HpaI GTT'AAC
Cuts at: 0 1728 2445
Size: 1728 717
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 696 1783 2445
Size: 84 612 1087 662
Fragments arranged by size:
1087 662 612 84
MscI TGG'CCA
Cuts at: 0 575 877 2445
Size: 575 302 1568
Fragments arranged by size:
1568 575 302
MslI CAynn'nnrTG
Cuts at: 0 1021 1679 1714 2445
Size: 1021 658 35 731
Fragments arranged by size:
1021 731 658 35
MspA1I CmG'CkG
Cuts at: 0 31 47 2445
Size: 31 16 2398
Fragments arranged by size:
2398 31 16
NaeI GCC'GGC
Cuts at: 0 42 2445
Size: 42 2403
NdeI CA'TA_TG
Cuts at: 0 600 2445
Size: 600 1845
NgoMIV G'CCGG_C
Cuts at: 0 40 2445
Size: 40 2405
PflMI CCAn_nnn'nTGG
Cuts at: 0 873 2445
Size: 873 1572
PfoI T'CCnGG_A
Cuts at: 0 2133 2445
Size: 2133 312
PpuMI rG'GwC_Cy
Cuts at: 0 1228 1465 2445
Size: 1228 237 980
Fragments arranged by size:
1228 980 237
SacI G_AGCT'C
Cuts at: 0 1180 2445
Size: 1180 1265
SacII CC_GC'GG
Cuts at: 0 32 2445
Size: 32 2413
SapI GCTCTTCn'nnn_
Cuts at: 0 1763 2445
Size: 1763 682
ScaI AGT'ACT
Cuts at: 0 2334 2445
Size: 2334 111
SexAI A'CCwGG_T
Cuts at: 0 393 2358 2445
Size: 393 1965 87
Fragments arranged by size:
1965 393 87
SfcI C'TryA_G
Cuts at: 0 461 839 2445
Size: 461 378 1606
Fragments arranged by size:
1606 461 378
SmaI CCC'GGG
Cuts at: 0 478 2445
Size: 478 1967
SmlI C'TyrA_G
Cuts at: 0 93 252 1644 2445
Size: 93 159 1392 801
Fragments arranged by size:
1392 801 159 93
SpeI A'CTAG_T
Cuts at: 0 955 2445
Size: 955 1490
StuI AGG'CCT
Cuts at: 0 1944 2445
Size: 1944 501
StyI C'CwwG_G
Cuts at: 0 1651 2292 2445
Size: 1651 641 153
Fragments arranged by size:
1651 641 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 231 820 1143 1223 1927 2445
Size: 231 589 323 80 704 518
Fragments arranged by size:
704 589 518 323 231 80
TatI w'GTAC_w
Cuts at: 0 1200 1588 2332 2435 2445
Size: 1200 388 744 103 10
Fragments arranged by size:
1200 744 388 103 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 527 559 2165 2197 2445
Size: 527 32 1606 32 248
Fragments arranged by size:
1606 527 248 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 674 2445
Size: 674 1771
XmaI C'CCGG_G
Cuts at: 0 476 2445
Size: 476 1969
Enzymes that do cut:
AcuI AflII AhdI ApoI AseI AvaI BamHI BanII
BbvCI BglII Bme1580I BmgBI BplI BpmI Bpu10I BpuEI
BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrFI BsrGI
BssSI BstAPI BstEII BstXI BstYI BtgI BtsI DraI
DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I FalI
HaeII Hin4I HincII HpaI MmeI MscI MslI MspA1I
NaeI NdeI NgoMIV PflMI PfoI PpuMI SacI SacII
SapI ScaI SexAI SfcI SmaI SmlI SpeI StuI
StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvrII BaeI BanI BbeI BbsI BcgI BciVI BclI
BfrBI BglI BlpI BmrI BmtI BsaI BsaAI BseYI
BsiWI BsrBI BsrDI BssHII BstBI BstZ17I Bsu36I BtgZI
ClaI CspCI DraIII EciI EcoNI EcoRI EcoRV FseI
FspI FspAI HindIII KasI KpnI MfeI MluI NarI
NcoI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SalI SanDI SbfI
SfiI SfoI SgfI SgrAI SnaBI SphI SrfI SspI
SwaI Tth111I XbaI XhoI XmnI ZraI