Restriction map for
pFN21AE4230
(Linear) (Six-base) MAPSORT of: KIEE4230.seq Check: 1546 from: 1 to: 2424
KIEE4230.orf
With 182 enzymes: SgfI *
July 3, 2012 18:50 ..
AccI GT'mk_AC
Cuts at: 0 1460 2424
Size: 1460 964
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 598 893 911 1328 1466 2424
Size: 18 580 295 18 417 138 958
Fragments arranged by size:
958 580 417 295 138 18 18
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 521 2297 2424
Size: 521 1776 127
Fragments arranged by size:
1776 521 127
AlwNI CAG_nnn'CTG
Cuts at: 0 51 1864 1909 2424
Size: 51 1813 45 515
Fragments arranged by size:
1813 515 51 45
ApaLI G'TGCA_C
Cuts at: 0 1604 2424
Size: 1604 820
ApoI r'AATT_y
Cuts at: 0 990 1344 1398 1612 2232 2370 2424
Size: 990 354 54 214 620 138 54
Fragments arranged by size:
990 620 354 214 138 54 54
AvaI C'yCGr_G
Cuts at: 0 462 666 753 1129 2424
Size: 462 204 87 376 1295
Fragments arranged by size:
1295 462 376 204 87
BanI G'GyrC_C
Cuts at: 0 513 1294 2116 2424
Size: 513 781 822 308
Fragments arranged by size:
822 781 513 308
BanII G_rGCy'C
Cuts at: 0 1046 1198 1226 1668 2424
Size: 1046 152 28 442 756
Fragments arranged by size:
1046 756 442 152 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 827 2131 2424
Size: 827 1304 293
Fragments arranged by size:
1304 827 293
BciVI GTATCCnnnnn_n'
Cuts at: 0 1306 2424
Size: 1306 1118
BfrBI ATG'CAT
Cuts at: 0 1431 2424
Size: 1431 993
BglII A'GATC_T
Cuts at: 0 1680 2109 2424
Size: 1680 429 315
Fragments arranged by size:
1680 429 315
Bme1580I G_kGCm'C
Cuts at: 0 1608 2424
Size: 1608 816
BmrI ACTGGGnnnn_n'
Cuts at: 0 879 1040 1588 2424
Size: 879 161 548 836
Fragments arranged by size:
879 836 548 161
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1527 1968 2424
Size: 1527 441 456
Fragments arranged by size:
1527 456 441
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 1963 2041 2424
Size: 362 1601 78 383
Fragments arranged by size:
1601 383 362 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 719 1903 2424
Size: 719 1184 521
Fragments arranged by size:
1184 719 521
BsaI GGTCTCn'nnnn_
Cuts at: 0 113 656 1784 2424
Size: 113 543 1128 640
Fragments arranged by size:
1128 640 543 113
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1254 1284 2424
Size: 1254 30 1140
Fragments arranged by size:
1254 1140 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 235 444 1722 1960 1972 1975 2424
Size: 235 209 1278 238 12 3 449
Fragments arranged by size:
1278 449 238 235 209 12 3
BseYI C'CCAG_C
Cuts at: 0 364 1105 2424
Size: 364 741 1319
Fragments arranged by size:
1319 741 364
BsiHKAI G_wGCw'C
Cuts at: 0 675 1608 2424
Size: 675 933 816
Fragments arranged by size:
933 816 675
BsmI GAATG_Cn'
Cuts at: 0 1431 2424
Size: 1431 993
BsmBI CGTCTCn'nnnn_
Cuts at: 0 93 2424
Size: 93 2331
Bsp1286I G_dGCh'C
Cuts at: 0 675 1046 1198 1226 1608 1668 2424
Size: 675 371 152 28 382 60 756
Fragments arranged by size:
756 675 382 371 152 60 28
BspHI T'CATG_A
Cuts at: 0 2291 2424
Size: 2291 133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 182 2424
Size: 182 2242
BsrBI CCG'CTC
Cuts at: 0 1095 2424
Size: 1095 1329
BsrDI GCAATG_nn'
Cuts at: 0 1077 1082 2424
Size: 1077 5 1342
Fragments arranged by size:
1342 1077 5
BsrFI r'CCGG_y
Cuts at: 0 516 2424
Size: 516 1908
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2151 2424
Size: 2151 273
BstYI r'GATC_y
Cuts at: 0 175 1680 2109 2424
Size: 175 1505 429 315
Fragments arranged by size:
1505 429 315 175
Bsu36I CC'TnA_GG
Cuts at: 0 427 974 1658 2424
Size: 427 547 684 766
Fragments arranged by size:
766 684 547 427
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 53 878 2424
Size: 53 825 1546
Fragments arranged by size:
1546 825 53
BtsI GCAGTG_nn'
Cuts at: 0 505 1182 1820 2424
Size: 505 677 638 604
Fragments arranged by size:
677 638 604 505
DraI TTT'AAA
Cuts at: 0 1331 2424
Size: 1331 1093
EarI CTCTTCn'nnn_
Cuts at: 0 111 574 849 869 2424
Size: 111 463 275 20 1555
Fragments arranged by size:
1555 463 275 111 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 362 598 893 911 1328 1466 1963
Size: 18 344 236 295 18 417 138 497
Cuts at: 1963 2041 2424
Size: 78 383
Fragments arranged by size:
497 417 383 344 295 236 138 78
18 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 706 1945 2424
Size: 706 1239 479
Fragments arranged by size:
1239 706 479
EcoRI G'AATT_C
Cuts at: 0 1398 2232 2424
Size: 1398 834 192
Fragments arranged by size:
1398 834 192
EcoRV GAT'ATC
Cuts at: 0 260 2424
Size: 260 2164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 289 321 425 457 573 605 1132 1164
Size: 289 32 104 32 116 32 527 32
Cuts at: 1164 1201 1233 2314 2346 2424
Size: 37 32 1081 32 78
Fragments arranged by size:
1081 527 289 116 104 78 37 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2160 2424
Size: 2160 264
MfeI C'AATT_G
Cuts at: 0 293 2424
Size: 293 2131
MslI CAynn'nnrTG
Cuts at: 0 270 1766 2267 2424
Size: 270 1496 501 157
Fragments arranged by size:
1496 501 270 157
NsiI A_TGCA'T
Cuts at: 0 1433 2424
Size: 1433 991
NspI r_CATG'y
Cuts at: 0 63 1771 2424
Size: 63 1708 653
Fragments arranged by size:
1708 653 63
PasI CC'CwG_GG
Cuts at: 0 524 2424
Size: 524 1900
PfoI T'CCnGG_A
Cuts at: 0 1940 2018 2424
Size: 1940 78 406
Fragments arranged by size:
1940 406 78
PshAI GACnn'nnGTC
Cuts at: 0 1973 2424
Size: 1973 451
PstI C_TGCA'G
Cuts at: 0 2051 2424
Size: 2051 373
SexAI A'CCwGG_T
Cuts at: 0 2246 2309 2424
Size: 2246 63 115
Fragments arranged by size:
2246 115 63
SfcI C'TryA_G
Cuts at: 0 779 1154 2047 2424
Size: 779 375 893 377
Fragments arranged by size:
893 779 377 375
SmaI CCC'GGG
Cuts at: 0 1131 2424
Size: 1131 1293
SmlI C'TyrA_G
Cuts at: 0 734 1918 2424
Size: 734 1184 506
Fragments arranged by size:
1184 734 506
StuI AGG'CCT
Cuts at: 0 557 712 1290 2150 2424
Size: 557 155 578 860 274
Fragments arranged by size:
860 578 557 274 155
StyI C'CwwG_G
Cuts at: 0 241 717 2424
Size: 241 476 1707
Fragments arranged by size:
1707 476 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 786 1312 2155 2424
Size: 786 526 843 269
Fragments arranged by size:
843 786 526 269
XmaI C'CCGG_G
Cuts at: 0 1129 2424
Size: 1129 1295
XmnI GAAnn'nnTTC
Cuts at: 0 31 2424
Size: 31 2393
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApaLI ApoI AvaI BanI
BanII BbsI BciVI BfrBI BglII Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaXI BseRI BseYI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI
BstYI Bsu36I BtgZI BtsI DraI EarI Eco57MI EcoNI
EcoRI EcoRV Hin4I HincII MfeI MslI NsiI NspI
PasI PfoI PshAI PstI SexAI SfcI SmaI SmlI
StuI StyI TaqII XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BclI BglI BlpI
BmgBI BmtI Bpu10I BsaAI BsaBI BsaHI BsaWI BsgI
BsiEI BsiWI BspEI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I BtgI ClaI CspCI DraIII DrdI EaeI
EagI EciI EcoICRI EcoO109I FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MluI MmeI MscI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PciI PflMI PmeI PmlI PpiI PpuMI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI Tth111I XbaI XcmI XhoI ZraI