Restriction map for
pFN21AE4223
(Linear) (Six-base) MAPSORT of: KIEE4223.seq Check: 6856 from: 1 to: 2328
KIEE4223.orf
With 182 enzymes: SgfI *
March 22, 2012 17:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 243 2328
Size: 243 2085
Acc65I G'GTAC_C
Cuts at: 0 18 2328
Size: 18 2310
AclI AA'CG_TT
Cuts at: 0 472 2328
Size: 472 1856
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 920 2328
Size: 920 1408
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1916 2328
Size: 1916 412
ApoI r'AATT_y
Cuts at: 0 164 562 904 1412 1501 1517 1768 1814
Size: 164 398 342 508 89 16 251 46
Cuts at: 1814 1973 2297 2304 2328
Size: 159 324 7 24
Fragments arranged by size:
508 398 342 324 251 164 159 89
46 24 16 7
AseI AT'TA_AT
Cuts at: 0 554 2328
Size: 554 1774
BanI G'GyrC_C
Cuts at: 0 18 121 2328
Size: 18 103 2207
Fragments arranged by size:
2207 103 18
BanII G_rGCy'C
Cuts at: 0 1600 2328
Size: 1600 728
BbeI G_GCGC'C
Cuts at: 0 125 2328
Size: 125 2203
BbsI GAAGACnn'nnnn_
Cuts at: 0 548 622 1224 2328
Size: 548 74 602 1104
Fragments arranged by size:
1104 602 548 74
BclI T'GATC_A
Cuts at: 0 1617 2288 2328
Size: 1617 671 40
Fragments arranged by size:
1617 671 40
BfrBI ATG'CAT
Cuts at: 0 328 2007 2328
Size: 328 1679 321
Fragments arranged by size:
1679 328 321
BmrI ACTGGGnnnn_n'
Cuts at: 0 346 2328
Size: 346 1982
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2042 2328
Size: 2042 286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 2328
Size: 587 1741
BsaI GGTCTCn'nnnn_
Cuts at: 0 1218 2328
Size: 1218 1110
BsaAI yAC'GTr
Cuts at: 0 737 2328
Size: 737 1591
BsaBI GATnn'nnATC
Cuts at: 0 461 1180 2328
Size: 461 719 1148
Fragments arranged by size:
1148 719 461
BsaHI Gr'CG_yC
Cuts at: 0 122 2328
Size: 122 2206
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 79 164 590 2328
Size: 79 85 426 1738
Fragments arranged by size:
1738 426 85 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 2328
Size: 221 2107
BsiEI CG_ry'CG
Cuts at: 0 56 134 2328
Size: 56 78 2194
Fragments arranged by size:
2194 78 56
BsmI GAATG_Cn'
Cuts at: 0 576 2328
Size: 576 1752
Bsp1286I G_dGCh'C
Cuts at: 0 1600 2328
Size: 1600 728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 243 2236 2328
Size: 243 1993 92
Fragments arranged by size:
1993 243 92
BsrDI GCAATG_nn'
Cuts at: 0 1650 1728 2328
Size: 1650 78 600
Fragments arranged by size:
1650 600 78
BsrFI r'CCGG_y
Cuts at: 0 124 2328
Size: 124 2204
BsrGI T'GTAC_A
Cuts at: 0 1848 2328
Size: 1848 480
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2013 2328
Size: 2013 315
BstXI CCAn_nnnn'nTGG
Cuts at: 0 39 2328
Size: 39 2289
BstYI r'GATC_y
Cuts at: 0 2271 2328
Size: 2271 57
Bsu36I CC'TnA_GG
Cuts at: 0 62 2328
Size: 62 2266
BtgI C'CryG_G
Cuts at: 0 134 1652 1689 2328
Size: 134 1518 37 639
Fragments arranged by size:
1518 639 134 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1167 2328
Size: 1167 1161
BtsI GCAGTG_nn'
Cuts at: 0 518 1458 2328
Size: 518 940 870
Fragments arranged by size:
940 870 518
DraI TTT'AAA
Cuts at: 0 816 1482 1796 2178 2328
Size: 816 666 314 382 150
Fragments arranged by size:
816 666 382 314 150
EaeI y'GGCC_r
Cuts at: 0 53 2328
Size: 53 2275
EagI C'GGCC_G
Cuts at: 0 53 2328
Size: 53 2275
EarI CTCTTCn'nnn_
Cuts at: 0 826 1958 2328
Size: 826 1132 370
Fragments arranged by size:
1132 826 370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 920 2328
Size: 920 1408
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2153 2328
Size: 2153 175
EcoO109I rG'GnC_Cy
Cuts at: 0 348 2328
Size: 348 1980
EcoRI G'AATT_C
Cuts at: 0 1501 1517 2328
Size: 1501 16 811
Fragments arranged by size:
1501 811 16
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 700 2328
Size: 700 1628
HaeII r_GCGC'y
Cuts at: 0 125 2328
Size: 125 2203
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 316 348 1195 1227 2328
Size: 316 32 847 32 1101
Fragments arranged by size:
1101 847 316 32 32
KasI G'GCGC_C
Cuts at: 0 121 2328
Size: 121 2207
KpnI G_GTAC'C
Cuts at: 0 22 2328
Size: 22 2306
MfeI C'AATT_G
Cuts at: 0 844 2328
Size: 844 1484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1494 2328
Size: 1494 834
MspA1I CmG'CkG
Cuts at: 0 39 136 193 2328
Size: 39 97 57 2135
Fragments arranged by size:
2135 97 57 39
NaeI GCC'GGC
Cuts at: 0 126 2328
Size: 126 2202
NarI GG'CG_CC
Cuts at: 0 122 2328
Size: 122 2206
NcoI C'CATG_G
Cuts at: 0 1689 2328
Size: 1689 639
NgoMIV G'CCGG_C
Cuts at: 0 124 2328
Size: 124 2204
NotI GC'GGCC_GC
Cuts at: 0 53 2328
Size: 53 2275
NsiI A_TGCA'T
Cuts at: 0 330 2009 2328
Size: 330 1679 319
Fragments arranged by size:
1679 330 319
NspI r_CATG'y
Cuts at: 0 1437 2011 2328
Size: 1437 574 317
Fragments arranged by size:
1437 574 317
PflMI CCAn_nnn'nTGG
Cuts at: 0 1429 2328
Size: 1429 899
PshAI GACnn'nnGTC
Cuts at: 0 1664 2328
Size: 1664 664
PsiI TTA'TAA
Cuts at: 0 1541 2328
Size: 1541 787
PstI C_TGCA'G
Cuts at: 0 2018 2328
Size: 2018 310
PvuII CAG'CTG
Cuts at: 0 193 2328
Size: 193 2135
SacII CC_GC'GG
Cuts at: 0 137 2328
Size: 137 2191
ScaI AGT'ACT
Cuts at: 0 2025 2328
Size: 2025 303
SfcI C'TryA_G
Cuts at: 0 1127 2014 2129 2150 2328
Size: 1127 887 115 21 178
Fragments arranged by size:
1127 887 178 115 21
SfoI GGC'GCC
Cuts at: 0 123 2328
Size: 123 2205
SgrAI Cr'CCGG_yG
Cuts at: 0 124 2328
Size: 124 2204
SmlI C'TyrA_G
Cuts at: 0 602 2323 2328
Size: 602 1721 5
Fragments arranged by size:
1721 602 5
SnaBI TAC'GTA
Cuts at: 0 737 2328
Size: 737 1591
SphI G_CATG'C
Cuts at: 0 2011 2328
Size: 2011 317
StyI C'CwwG_G
Cuts at: 0 1553 1689 2328
Size: 1553 136 639
Fragments arranged by size:
1553 639 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1360 2328
Size: 1360 968
TatI w'GTAC_w
Cuts at: 0 450 1848 2023 2328
Size: 450 1398 175 305
Fragments arranged by size:
1398 450 305 175
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1081 1113 2328
Size: 1081 32 1215
Fragments arranged by size:
1215 1081 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 39 2328
Size: 39 2289
XmnI GAAnn'nnTTC
Cuts at: 0 368 835 905 2328
Size: 368 467 70 1423
Fragments arranged by size:
1423 467 368 70
Enzymes that do cut:
AarI Acc65I AclI AcuI AlfI ApoI AseI BanI
BanII BbeI BbsI BclI BfrBI BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BseRI BsgI BsiEI BsmI
Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI EaeI EagI EarI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I KasI
KpnI MfeI MmeI MspA1I NaeI NarI NcoI NgoMIV
NotI NsiI NspI PflMI PshAI PsiI PstI PvuII
SacII ScaI SfcI SfoI SgrAI SmlI SnaBI SphI
StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI AfeI AflII AflIII AgeI AhdI AleI
AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BbvCI BcgI BciVI BglI BglII BlpI
Bme1580I BmgBI BmtI BpmI Bpu10I BsaWI BsaXI BseYI
BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BssHII BssSI
BstBI BstEII BstZ17I ClaI CspCI DraIII DrdI EciI
EcoICRI EcoRV FseI FspI FspAI HincII HindIII HpaI
MluI MscI MslI NdeI NheI NruI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI
PsrI PvuI RsrII SacI SalI SanDI SapI SbfI
SexAI SfiI SgfI SmaI SpeI SrfI SspI StuI
SwaI Tth111I XbaI XhoI XmaI ZraI