Restriction map for
pFN21AE4222
(Linear) (Six-base) MAPSORT of: KIEE4222.seq Check: 6743 from: 1 to: 2319
KIEE4222.orf
With 182 enzymes: SgfI *
July 3, 2012 18:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 948 2319
Size: 948 1371
AccI GT'mk_AC
Cuts at: 0 842 1794 2216 2319
Size: 842 952 422 103
Fragments arranged by size:
952 842 422 103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 536 548 555 831 892 1027 1121
Size: 211 325 12 7 276 61 135 94
Cuts at: 1121 2104 2143 2319
Size: 983 39 176
Fragments arranged by size:
983 325 276 211 176 135 94 61
39 12 7
AfeI AGC'GCT
Cuts at: 0 139 2319
Size: 139 2180
AhdI GACnn_n'nnGTC
Cuts at: 0 1905 2319
Size: 1905 414
AlwNI CAG_nnn'CTG
Cuts at: 0 337 425 1144 1243 2319
Size: 337 88 719 99 1076
Fragments arranged by size:
1076 719 337 99 88
ApaI G_GGCC'C
Cuts at: 0 237 636 2319
Size: 237 399 1683
Fragments arranged by size:
1683 399 237
ApoI r'AATT_y
Cuts at: 0 743 1188 2088 2114 2319
Size: 743 445 900 26 205
Fragments arranged by size:
900 743 445 205 26
AseI AT'TA_AT
Cuts at: 0 1411 1612 2319
Size: 1411 201 707
Fragments arranged by size:
1411 707 201
AvaI C'yCGr_G
Cuts at: 0 247 635 652 2140 2319
Size: 247 388 17 1488 179
Fragments arranged by size:
1488 388 247 179 17
BanI G'GyrC_C
Cuts at: 0 259 346 2319
Size: 259 87 1973
Fragments arranged by size:
1973 259 87
BanII G_rGCy'C
Cuts at: 0 237 359 636 2319
Size: 237 122 277 1683
Fragments arranged by size:
1683 277 237 122
BbeI G_GCGC'C
Cuts at: 0 263 350 2319
Size: 263 87 1969
Fragments arranged by size:
1969 263 87
BglI GCCn_nnn'nGGC
Cuts at: 0 640 706 2319
Size: 640 66 1613
Fragments arranged by size:
1613 640 66
BlpI GC'TnA_GC
Cuts at: 0 915 2319
Size: 915 1404
Bme1580I G_kGCm'C
Cuts at: 0 237 410 636 2319
Size: 237 173 226 1683
Fragments arranged by size:
1683 237 226 173
BmrI ACTGGGnnnn_n'
Cuts at: 0 719 2319
Size: 719 1600
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 223 333 1041 2319
Size: 133 90 110 708 1278
Fragments arranged by size:
1278 708 133 110 90
Bpu10I CC'TnA_GC
Cuts at: 0 388 1383 2319
Size: 388 995 936
Fragments arranged by size:
995 936 388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1528 2051 2319
Size: 1528 523 268
Fragments arranged by size:
1528 523 268
BsaI GGTCTCn'nnnn_
Cuts at: 0 775 2300 2319
Size: 775 1525 19
Fragments arranged by size:
1525 775 19
BsaHI Gr'CG_yC
Cuts at: 0 260 347 2319
Size: 260 87 1972
Fragments arranged by size:
1972 260 87
BsaWI w'CCGG_w
Cuts at: 0 1896 2319
Size: 1896 423
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1572 1602 2319
Size: 1572 30 717
Fragments arranged by size:
1572 717 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 349 2319
Size: 349 1970
BseYI C'CCAG_C
Cuts at: 0 54 140 177 306 2319
Size: 54 86 37 129 2013
Fragments arranged by size:
2013 129 86 54 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 2319
Size: 649 1670
BsiEI CG_ry'CG
Cuts at: 0 125 535 644 2319
Size: 125 410 109 1675
Fragments arranged by size:
1675 410 125 109
BsiHKAI G_wGCw'C
Cuts at: 0 1170 2319
Size: 1170 1149
Bsp1286I G_dGCh'C
Cuts at: 0 237 359 410 636 1170 2319
Size: 237 122 51 226 534 1149
Fragments arranged by size:
1149 534 237 226 122 51
BspHI T'CATG_A
Cuts at: 0 2049 2319
Size: 2049 270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 948 2187 2319
Size: 948 1239 132
Fragments arranged by size:
1239 948 132
BsrBI CCG'CTC
Cuts at: 0 630 754 2319
Size: 630 124 1565
Fragments arranged by size:
1565 630 124
BsrFI r'CCGG_y
Cuts at: 0 74 290 349 396 479 643 2319
Size: 74 216 59 47 83 164 1676
Fragments arranged by size:
1676 216 164 83 74 59 47
BssHII G'CGCG_C
Cuts at: 0 117 197 2319
Size: 117 80 2122
Fragments arranged by size:
2122 117 80
BstBI TT'CG_AA
Cuts at: 0 1785 2106 2319
Size: 1785 321 213
Fragments arranged by size:
1785 321 213
BstEII G'GTnAC_C
Cuts at: 0 399 2319
Size: 399 1920
BstZ17I GTA'TAC
Cuts at: 0 1795 2319
Size: 1795 524
Bsu36I CC'TnA_GG
Cuts at: 0 836 2319
Size: 836 1483
BtgI C'CryG_G
Cuts at: 0 125 159 228 300 529 2319
Size: 125 34 69 72 229 1790
Fragments arranged by size:
1790 229 125 72 69 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 540 884 2319
Size: 540 344 1435
Fragments arranged by size:
1435 540 344
BtsI GCAGTG_nn'
Cuts at: 0 1976 2319
Size: 1976 343
ClaI AT'CG_AT
Cuts at: 0 1415 2319
Size: 1415 904
DraI TTT'AAA
Cuts at: 0 783 803 1223 1949 2319
Size: 783 20 420 726 370
Fragments arranged by size:
783 726 420 370 20
EaeI y'GGCC_r
Cuts at: 0 102 122 183 239 414 532 545 599
Size: 102 20 61 56 175 118 13 54
Cuts at: 599 641 692 2319
Size: 42 51 1627
Fragments arranged by size:
1627 175 118 102 61 56 54 51
42 20 13
EagI C'GGCC_G
Cuts at: 0 122 532 641 2319
Size: 122 410 109 1678
Fragments arranged by size:
1678 410 122 109
EarI CTCTTCn'nnn_
Cuts at: 0 1070 2319
Size: 1070 1249
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 145 286 2319
Size: 145 141 2033
Fragments arranged by size:
2033 145 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 211 223 333 536 548 555 831
Size: 133 78 12 110 203 12 7 276
Cuts at: 831 892 1027 1041 1121 2104 2143 2319
Size: 61 135 14 80 983 39 176
Fragments arranged by size:
983 276 203 176 135 133 110 80
78 61 39 14 12 12 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1148 2319
Size: 1148 1171
EcoO109I rG'GnC_Cy
Cuts at: 0 23 233 234 687 2319
Size: 23 210 1 453 1632
Fragments arranged by size:
1632 453 210 23 1
HaeII r_GCGC'y
Cuts at: 0 61 141 178 223 263 338 350 678
Size: 61 80 37 45 40 75 12 328
Cuts at: 678 712 2319
Size: 34 1607
Fragments arranged by size:
1607 328 80 75 61 45 40 37
34 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1069 1101 1624 1656 1891 1923 2019 2051
Size: 1069 32 523 32 235 32 96 32
Cuts at: 2051 2319
Size: 268
Fragments arranged by size:
1069 523 268 235 96 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2270 2319
Size: 2270 49
HindIII A'AGCT_T
Cuts at: 0 1155 1554 2319
Size: 1155 399 765
Fragments arranged by size:
1155 765 399
KasI G'GCGC_C
Cuts at: 0 259 346 2319
Size: 259 87 1973
Fragments arranged by size:
1973 259 87
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 1583 1905 2319
Size: 113 1470 322 414
Fragments arranged by size:
1470 414 322 113
MscI TGG'CCA
Cuts at: 0 416 2319
Size: 416 1903
MslI CAynn'nnrTG
Cuts at: 0 1032 2319
Size: 1032 1287
MspA1I CmG'CkG
Cuts at: 0 101 127 161 230 434 461 531 1599
Size: 101 26 34 69 204 27 70 1068
Cuts at: 1599 2078 2232 2319
Size: 479 154 87
Fragments arranged by size:
1068 479 204 154 101 87 70 69
34 27 26
NaeI GCC'GGC
Cuts at: 0 351 645 2319
Size: 351 294 1674
Fragments arranged by size:
1674 351 294
NarI GG'CG_CC
Cuts at: 0 260 347 2319
Size: 260 87 1972
Fragments arranged by size:
1972 260 87
NdeI CA'TA_TG
Cuts at: 0 762 2319
Size: 762 1557
NgoMIV G'CCGG_C
Cuts at: 0 349 643 2319
Size: 349 294 1676
Fragments arranged by size:
1676 349 294
NotI GC'GGCC_GC
Cuts at: 0 122 532 2319
Size: 122 410 1787
Fragments arranged by size:
1787 410 122
NspI r_CATG'y
Cuts at: 0 1824 2319
Size: 1824 495
PflMI CCAn_nnn'nTGG
Cuts at: 0 111 306 2319
Size: 111 195 2013
Fragments arranged by size:
2013 195 111
PfoI T'CCnGG_A
Cuts at: 0 1018 2319
Size: 1018 1301
PpuMI rG'GwC_Cy
Cuts at: 0 23 2319
Size: 23 2296
PspOMI G'GGCC_C
Cuts at: 0 233 632 2319
Size: 233 399 1687
Fragments arranged by size:
1687 399 233
PstI C_TGCA'G
Cuts at: 0 2077 2319
Size: 2077 242
PvuII CAG'CTG
Cuts at: 0 1599 2078 2232 2319
Size: 1599 479 154 87
Fragments arranged by size:
1599 479 154 87
SacII CC_GC'GG
Cuts at: 0 128 162 231 532 2319
Size: 128 34 69 301 1787
Fragments arranged by size:
1787 301 128 69 34
ScaI AGT'ACT
Cuts at: 0 863 2319
Size: 863 1456
SfcI C'TryA_G
Cuts at: 0 2073 2213 2319
Size: 2073 140 106
Fragments arranged by size:
2073 140 106
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 640 2319
Size: 640 1679
SfoI GGC'GCC
Cuts at: 0 261 348 2319
Size: 261 87 1971
Fragments arranged by size:
1971 261 87
SgrAI Cr'CCGG_yG
Cuts at: 0 349 2319
Size: 349 1970
SmaI CCC'GGG
Cuts at: 0 637 2319
Size: 637 1682
SmlI C'TyrA_G
Cuts at: 0 1507 2066 2319
Size: 1507 559 253
Fragments arranged by size:
1507 559 253
SrfI GCCC'GGGC
Cuts at: 0 637 2319
Size: 637 1682
SspI AAT'ATT
Cuts at: 0 1709 2319
Size: 1709 610
StuI AGG'CCT
Cuts at: 0 1004 2319
Size: 1004 1315
SwaI ATTT'AAAT
Cuts at: 0 803 2319
Size: 803 1516
TatI w'GTAC_w
Cuts at: 0 861 954 1036 2319
Size: 861 93 82 1283
Fragments arranged by size:
1283 861 93 82
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1572 1604 1895 1927 2319
Size: 1572 32 291 32 392
Fragments arranged by size:
1572 392 291 32 32
XbaI T'CTAG_A
Cuts at: 0 2302 2319
Size: 2302 17
XmaI C'CCGG_G
Cuts at: 0 635 2319
Size: 635 1684
XmnI GAAnn'nnTTC
Cuts at: 0 1244 2156 2319
Size: 1244 912 163
Fragments arranged by size:
1244 912 163
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AlwNI ApaI ApoI
AseI AvaI BanI BanII BbeI BglI BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrFI BssHII BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI DraI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII KasI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NotI NspI PflMI PfoI PpuMI PspOMI PstI PvuII
SacII ScaI SfcI SfiI SfoI SgrAI SmaI SmlI
SrfI SspI StuI SwaI TatI TstI XbaI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AleI AlfI
AloI ApaLI AscI AsiSI AvrII BaeI BamHI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI
BplI BsaAI BsaBI BsiWI BsmI BsmBI BspEI BsrDI
BsrGI BssSI BstAPI BstXI BstYI CspCI DraIII DrdI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HpaI
KpnI MfeI MluI NcoI NheI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SalI SanDI SapI SbfI
SexAI SgfI SnaBI SpeI SphI StyI TaqII Tth111I
XcmI XhoI ZraI