Restriction map for
pFN21AE4220
(Linear) (Six-base) MAPSORT of: KIEE4220.seq Check: 6619 from: 1 to: 2277
KIEE4220.orf
With 182 enzymes: SgfI *
July 3, 2012 18:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1380 1574 2277
Size: 1380 194 703
Fragments arranged by size:
1380 703 194
AccI GT'mk_AC
Cuts at: 0 1793 2277
Size: 1793 484
Acc65I G'GTAC_C
Cuts at: 0 476 2277
Size: 476 1801
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 979 1199 2277
Size: 979 220 1078
Fragments arranged by size:
1078 979 220
AflII C'TTAA_G
Cuts at: 0 316 1108 2277
Size: 316 792 1169
Fragments arranged by size:
1169 792 316
AflIII A'CryG_T
Cuts at: 0 152 2277
Size: 152 2125
AleI CACnn'nnGTG
Cuts at: 0 784 907 1408 1727 2277
Size: 784 123 501 319 550
Fragments arranged by size:
784 550 501 319 123
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1426 1999 2277
Size: 1426 573 278
Fragments arranged by size:
1426 573 278
AlwNI CAG_nnn'CTG
Cuts at: 0 129 1585 1778 2167 2277
Size: 129 1456 193 389 110
Fragments arranged by size:
1456 389 193 129 110
ApaLI G'TGCA_C
Cuts at: 0 1561 2277
Size: 1561 716
AvaI C'yCGr_G
Cuts at: 0 170 1169 2277
Size: 170 999 1108
Fragments arranged by size:
1108 999 170
BanI G'GyrC_C
Cuts at: 0 476 502 613 1462 1861 1915 2277
Size: 476 26 111 849 399 54 362
Fragments arranged by size:
849 476 399 362 111 54 26
BanII G_rGCy'C
Cuts at: 0 230 638 1498 2138 2277
Size: 230 408 860 640 139
Fragments arranged by size:
860 640 408 230 139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1897 1931 2277
Size: 1897 34 346
Fragments arranged by size:
1897 346 34
BclI T'GATC_A
Cuts at: 0 374 1329 1874 2277
Size: 374 955 545 403
Fragments arranged by size:
955 545 403 374
BglI GCCn_nnn'nGGC
Cuts at: 0 191 802 1616 2277
Size: 191 611 814 661
Fragments arranged by size:
814 661 611 191
BglII A'GATC_T
Cuts at: 0 872 1334 2277
Size: 872 462 943
Fragments arranged by size:
943 872 462
BlpI GC'TnA_GC
Cuts at: 0 1418 2277
Size: 1418 859
Bme1580I G_kGCm'C
Cuts at: 0 100 171 391 616 682 773 1465 1473
Size: 100 71 220 225 66 91 692 8
Cuts at: 1473 1565 1866 1907 2057 2149 2176 2230 2277
Size: 92 301 41 150 92 27 54 47
Fragments arranged by size:
692 301 225 220 150 100 92 92
91 71 66 54 47 41 27 8
BmgBI CAC'GTC
Cuts at: 0 1239 1246 1752 2277
Size: 1239 7 506 525
Fragments arranged by size:
1239 525 506 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 94 1788 2277
Size: 94 1694 489
Fragments arranged by size:
1694 489 94
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 249 2277
Size: 249 2028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 904 991 1171 1852 2197 2277
Size: 478 426 87 180 681 345 80
Fragments arranged by size:
681 478 426 345 180 87 80
Bpu10I CC'TnA_GC
Cuts at: 0 1077 2160 2277
Size: 1077 1083 117
Fragments arranged by size:
1083 1077 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 1498 2277
Size: 793 705 779
Fragments arranged by size:
793 779 705
BsaHI Gr'CG_yC
Cuts at: 0 1823 1939 2277
Size: 1823 116 338
Fragments arranged by size:
1823 338 116
BsaWI w'CCGG_w
Cuts at: 0 443 1058 2277
Size: 443 615 1219
Fragments arranged by size:
1219 615 443
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 205 235 922 952 1786 1816 2277
Size: 205 30 687 30 834 30 461
Fragments arranged by size:
834 687 461 205 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 901 946 988 1203 1465 2277
Size: 901 45 42 215 262 812
Fragments arranged by size:
901 812 262 215 45 42
BseYI C'CCAG_C
Cuts at: 0 217 390 626 957 1310 1578 1613 1797
Size: 217 173 236 331 353 268 35 184
Cuts at: 1797 1986 2277
Size: 189 291
Fragments arranged by size:
353 331 291 268 236 217 189 184
173 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 1138 1552 1576 2031 2277
Size: 540 598 414 24 455 246
Fragments arranged by size:
598 540 455 414 246 24
BsiHKAI G_wGCw'C
Cuts at: 0 791 1058 1565 2277
Size: 791 267 507 712
Fragments arranged by size:
791 712 507 267
BsmI GAATG_Cn'
Cuts at: 0 1015 2277
Size: 1015 1262
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1039 2277
Size: 1039 1238
Bsp1286I G_dGCh'C
Cuts at: 0 100 171 230 391 616 638 682 773
Size: 100 71 59 161 225 22 44 91
Cuts at: 773 791 1058 1465 1473 1498 1565 1866 1907
Size: 18 267 407 8 25 67 301 41
Cuts at: 1907 2057 2138 2149 2176 2230 2277
Size: 150 81 11 27 54 47
Fragments arranged by size:
407 301 267 225 161 150 100 91
81 71 67 59 54 47 44 41
27 25 22 18 11 8
BspEI T'CCGG_A
Cuts at: 0 443 2277
Size: 443 1834
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1380 1574 2277
Size: 1380 194 703
Fragments arranged by size:
1380 703 194
BsrBI CCG'CTC
Cuts at: 0 423 474 1023 1632 2094 2277
Size: 423 51 549 609 462 183
Fragments arranged by size:
609 549 462 423 183 51
BsrDI GCAATG_nn'
Cuts at: 0 588 1224 2277
Size: 588 636 1053
Fragments arranged by size:
1053 636 588
BsrFI r'CCGG_y
Cuts at: 0 308 1534 1703 1714 2041 2277
Size: 308 1226 169 11 327 236
Fragments arranged by size:
1226 327 308 236 169 11
BsrGI T'GTAC_A
Cuts at: 0 116 2277
Size: 116 2161
BstAPI GCAn_nnn'nTGC
Cuts at: 0 563 587 719 948 2277
Size: 563 24 132 229 1329
Fragments arranged by size:
1329 563 229 132 24
BstEII G'GTnAC_C
Cuts at: 0 699 2277
Size: 699 1578
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1376 1971 2277
Size: 1376 595 306
Fragments arranged by size:
1376 595 306
BstYI r'GATC_y
Cuts at: 0 872 1046 1334 1979 2277
Size: 872 174 288 645 298
Fragments arranged by size:
872 645 298 288 174
Bsu36I CC'TnA_GG
Cuts at: 0 1289 2277
Size: 1289 988
BtgI C'CryG_G
Cuts at: 0 1518 2130 2277
Size: 1518 612 147
Fragments arranged by size:
1518 612 147
BtsI GCAGTG_nn'
Cuts at: 0 988 2277
Size: 988 1289
DraIII CAC_nnn'GTG
Cuts at: 0 153 563 2277
Size: 153 410 1714
Fragments arranged by size:
1714 410 153
DrdI GACnn_nn'nnGTC
Cuts at: 0 49 2277
Size: 49 2228
EaeI y'GGCC_r
Cuts at: 0 1198 2277
Size: 1198 1079
EarI CTCTTCn'nnn_
Cuts at: 0 604 689 2277
Size: 604 85 1588
Fragments arranged by size:
1588 604 85
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1210 2277
Size: 1210 1067
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 904 979 991 1171 1199 1852 2197
Size: 478 426 75 12 180 28 653 345
Cuts at: 2197 2277
Size: 80
Fragments arranged by size:
653 478 426 345 180 80 75 28
12
EcoO109I rG'GnC_Cy
Cuts at: 0 213 669 804 1293 1426 2164 2277
Size: 213 456 135 489 133 738 113
Fragments arranged by size:
738 489 456 213 135 133 113
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 342 2277
Size: 342 1935
HaeII r_GCGC'y
Cuts at: 0 1043 2277
Size: 1043 1234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 491 523 922 954 1480 1512
Size: 205 32 254 32 399 32 526 32
Cuts at: 1512 1786 1818 2277
Size: 274 32 459
Fragments arranged by size:
526 459 399 274 254 205 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 739 2277
Size: 739 1538
HindIII A'AGCT_T
Cuts at: 0 1840 2277
Size: 1840 437
KpnI G_GTAC'C
Cuts at: 0 480 2277
Size: 480 1797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 684 2277
Size: 684 1593
MslI CAynn'nnrTG
Cuts at: 0 784 907 1408 1727 1969 2277
Size: 784 123 501 319 242 308
Fragments arranged by size:
784 501 319 308 242 123
MspA1I CmG'CkG
Cuts at: 0 767 945 1152 1204 1436 1443 1520 1613
Size: 767 178 207 52 232 7 77 93
Cuts at: 1613 1901 1913 2277
Size: 288 12 364
Fragments arranged by size:
767 364 288 232 207 178 93 77
52 12 7
NaeI GCC'GGC
Cuts at: 0 1536 2043 2277
Size: 1536 507 234
Fragments arranged by size:
1536 507 234
NgoMIV G'CCGG_C
Cuts at: 0 1534 2041 2277
Size: 1534 507 236
Fragments arranged by size:
1534 507 236
NspI r_CATG'y
Cuts at: 0 722 2277
Size: 722 1555
PasI CC'CwG_GG
Cuts at: 0 340 608 1764 2277
Size: 340 268 1156 513
Fragments arranged by size:
1156 513 340 268
PflMI CCAn_nnn'nTGG
Cuts at: 0 729 1436 2277
Size: 729 707 841
Fragments arranged by size:
841 729 707
PfoI T'CCnGG_A
Cuts at: 0 1049 2111 2213 2277
Size: 1049 1062 102 64
Fragments arranged by size:
1062 1049 102 64
PpuMI rG'GwC_Cy
Cuts at: 0 213 2277
Size: 213 2064
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1655 1687 2277
Size: 1655 32 590
Fragments arranged by size:
1655 590 32
PvuII CAG'CTG
Cuts at: 0 945 1152 1204 2277
Size: 945 207 52 1073
Fragments arranged by size:
1073 945 207 52
SacII CC_GC'GG
Cuts at: 0 1521 2277
Size: 1521 756
SanDI GG'GwC_CC
Cuts at: 0 213 2277
Size: 213 2064
SapI GCTCTTCn'nnn_
Cuts at: 0 604 2277
Size: 604 1673
ScaI AGT'ACT
Cuts at: 0 1106 2277
Size: 1106 1171
SmlI C'TyrA_G
Cuts at: 0 316 808 1108 1477 2277
Size: 316 492 300 369 800
Fragments arranged by size:
800 492 369 316 300
StuI AGG'CCT
Cuts at: 0 163 2277
Size: 163 2114
StyI C'CwwG_G
Cuts at: 0 399 548 665 926 2277
Size: 399 149 117 261 1351
Fragments arranged by size:
1351 399 261 149 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 631 775 1324 2277
Size: 631 144 549 953
Fragments arranged by size:
953 631 549 144
TatI w'GTAC_w
Cuts at: 0 116 1104 2205 2277
Size: 116 988 1101 72
Fragments arranged by size:
1101 988 116 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 79 111 339 371 2277
Size: 79 32 228 32 1906
Fragments arranged by size:
1906 228 79 32 32
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AleI AlfI
AlwNI ApaLI AvaI BanI BanII BcgI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
DraIII DrdI EaeI EarI EciI Eco57MI EcoO109I FalI
HaeII Hin4I HincII HindIII KpnI MmeI MslI MspA1I
NaeI NgoMIV NspI PasI PflMI PfoI PpuMI PsrI
PvuII SacII SanDI SapI ScaI SmlI StuI StyI
TaqII TatI TstI
Enzymes that do not cut:
AatII AclI AfeI AgeI AhdI AloI ApaI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI
BbvCI BciVI BfrBI BmtI BsaI BsaAI BsaBI BsiEI
BsiWI BspHI BssHII BssSI BstBI BstZ17I BtgZI ClaI
CspCI DraI EagI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HpaI KasI MfeI MluI MscI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI RsrII SacI SalI SbfI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI