Restriction map for
pFN21AE4216
(Linear) (Six-base) MAPSORT of: KIEE4216.seq Check: 4271 from: 1 to: 2244
KIEE4216.orf
With 182 enzymes: SgfI *
March 22, 2012 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 721 744 1738 2244
Size: 721 23 994 506
Fragments arranged by size:
994 721 506 23
Acc65I G'GTAC_C
Cuts at: 0 1405 2244
Size: 1405 839
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1296 1414 2244
Size: 1296 118 830
Fragments arranged by size:
1296 830 118
AflII C'TTAA_G
Cuts at: 0 631 2244
Size: 631 1613
AleI CACnn'nnGTG
Cuts at: 0 934 1841 2244
Size: 934 907 403
Fragments arranged by size:
934 907 403
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1 33 1153 1185 1690 1722 2244
Size: 1 32 1120 32 505 32 522
Fragments arranged by size:
1120 522 505 32 32 32 1
AlwNI CAG_nnn'CTG
Cuts at: 0 408 2244
Size: 408 1836
ApaI G_GGCC'C
Cuts at: 0 1787 2244
Size: 1787 457
ApaLI G'TGCA_C
Cuts at: 0 1925 2244
Size: 1925 319
ApoI r'AATT_y
Cuts at: 0 1043 2244
Size: 1043 1201
AvaI C'yCGr_G
Cuts at: 0 831 1474 2244
Size: 831 643 770
Fragments arranged by size:
831 770 643
BamHI G'GATC_C
Cuts at: 0 1168 2244
Size: 1168 1076
BanI G'GyrC_C
Cuts at: 0 1405 2244
Size: 1405 839
BanII G_rGCy'C
Cuts at: 0 557 1387 1787 2244
Size: 557 830 400 457
Fragments arranged by size:
830 557 457 400
BbsI GAAGACnn'nnnn_
Cuts at: 0 347 2244
Size: 347 1897
BbvCI CC'TCA_GC
Cuts at: 0 76 2244
Size: 76 2168
BciVI GTATCCnnnnn_n'
Cuts at: 0 408 2244
Size: 408 1836
BlpI GC'TnA_GC
Cuts at: 0 712 2244
Size: 712 1532
Bme1580I G_kGCm'C
Cuts at: 0 931 1420 1787 1929 2244
Size: 931 489 367 142 315
Fragments arranged by size:
931 489 367 315 142
BmrI ACTGGGnnnn_n'
Cuts at: 0 354 2244
Size: 354 1890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 1580 2244
Size: 430 1150 664
Fragments arranged by size:
1150 664 430
Bpu10I CC'TnA_GC
Cuts at: 0 76 2244
Size: 76 2168
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 775 1357 2244
Size: 112 663 582 887
Fragments arranged by size:
887 663 582 112
BsaI GGTCTCn'nnnn_
Cuts at: 0 520 2244
Size: 520 1724
BsaBI GATnn'nnATC
Cuts at: 0 278 2244
Size: 278 1966
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1 31 80 110 1690 1720 2244
Size: 1 30 49 30 1580 30 524
Fragments arranged by size:
1580 524 49 30 30 30 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 301 401 778 2244
Size: 19 282 100 377 1466
Fragments arranged by size:
1466 377 282 100 19
BseYI C'CCAG_C
Cuts at: 0 1269 2244
Size: 1269 975
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1915 2244
Size: 874 1041 329
Fragments arranged by size:
1041 874 329
BsiEI CG_ry'CG
Cuts at: 0 276 2244
Size: 276 1968
BsiHKAI G_wGCw'C
Cuts at: 0 128 557 1123 1387 1929 2244
Size: 128 429 566 264 542 315
Fragments arranged by size:
566 542 429 315 264 128
BsmI GAATG_Cn'
Cuts at: 0 60 2244
Size: 60 2184
Bsp1286I G_dGCh'C
Cuts at: 0 128 557 931 1123 1387 1420 1787 1929
Size: 128 429 374 192 264 33 367 142
Cuts at: 1929 2244
Size: 315
Fragments arranged by size:
429 374 367 315 264 192 142 128
33
BspHI T'CATG_A
Cuts at: 0 297 2244
Size: 297 1947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 721 744 1738 2244
Size: 721 23 994 506
Fragments arranged by size:
994 721 506 23
BsrDI GCAATG_nn'
Cuts at: 0 231 2244
Size: 231 2013
BsrGI T'GTAC_A
Cuts at: 0 609 1428 2244
Size: 609 819 816
Fragments arranged by size:
819 816 609
BstBI TT'CG_AA
Cuts at: 0 1494 2244
Size: 1494 750
BstXI CCAn_nnnn'nTGG
Cuts at: 0 177 2244
Size: 177 2067
BstYI r'GATC_y
Cuts at: 0 906 1168 2244
Size: 906 262 1076
Fragments arranged by size:
1076 906 262
Bsu36I CC'TnA_GG
Cuts at: 0 1531 2244
Size: 1531 713
BtgI C'CryG_G
Cuts at: 0 20 1061 2244
Size: 20 1041 1183
Fragments arranged by size:
1183 1041 20
BtsI GCAGTG_nn'
Cuts at: 0 478 1123 1884 1927 2244
Size: 478 645 761 43 317
Fragments arranged by size:
761 645 478 317 43
DraIII CAC_nnn'GTG
Cuts at: 0 618 2244
Size: 618 1626
EarI CTCTTCn'nnn_
Cuts at: 0 224 427 503 716 754 1392 1722 2244
Size: 224 203 76 213 38 638 330 522
Fragments arranged by size:
638 522 330 224 213 203 76 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 349 2244
Size: 349 1895
EcoICRI GAG'CTC
Cuts at: 0 555 1385 2244
Size: 555 830 859
Fragments arranged by size:
859 830 555
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 1296 1414 1580 2244
Size: 430 866 118 166 664
Fragments arranged by size:
866 664 430 166 118
EcoNI CCTnn'n_nnAGG
Cuts at: 0 383 1954 2244
Size: 383 1571 290
Fragments arranged by size:
1571 383 290
EcoO109I rG'GnC_Cy
Cuts at: 0 376 1307 1767 1784 2244
Size: 376 931 460 17 460
Fragments arranged by size:
931 460 460 376 17
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1403 2244
Size: 1403 841
FspI TGC'GCA
Cuts at: 0 1447 2244
Size: 1447 797
FspAI rTGC'GCAy
Cuts at: 0 1447 2244
Size: 1447 797
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 80 112 279 311 571 603 679 711
Size: 80 32 167 32 260 32 76 32
Cuts at: 711 1972 2004 2244
Size: 1261 32 240
Fragments arranged by size:
1261 260 240 167 80 76 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 840 2244
Size: 840 1404
HpaI GTT'AAC
Cuts at: 0 840 2244
Size: 840 1404
KpnI G_GTAC'C
Cuts at: 0 1409 2244
Size: 1409 835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 1727 2244
Size: 513 1214 517
Fragments arranged by size:
1214 517 513
MslI CAynn'nnrTG
Cuts at: 0 934 1841 2244
Size: 934 907 403
Fragments arranged by size:
934 907 403
NcoI C'CATG_G
Cuts at: 0 1061 2244
Size: 1061 1183
NdeI CA'TA_TG
Cuts at: 0 370 2244
Size: 370 1874
NspI r_CATG'y
Cuts at: 0 2151 2244
Size: 2151 93
PasI CC'CwG_GG
Cuts at: 0 238 1811 2244
Size: 238 1573 433
Fragments arranged by size:
1573 433 238
PfoI T'CCnGG_A
Cuts at: 0 264 1990 2244
Size: 264 1726 254
Fragments arranged by size:
1726 264 254
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 1690 1722 2244
Size: 1 32 1657 32 522
Fragments arranged by size:
1657 522 32 32 1
PpuMI rG'GwC_Cy
Cuts at: 0 376 1307 1767 2244
Size: 376 931 460 477
Fragments arranged by size:
931 477 460 376
PspOMI G'GGCC_C
Cuts at: 0 1783 2244
Size: 1783 461
PvuI CG_AT'CG
Cuts at: 0 276 2244
Size: 276 1968
SacI G_AGCT'C
Cuts at: 0 557 1387 2244
Size: 557 830 857
Fragments arranged by size:
857 830 557
SapI GCTCTTCn'nnn_
Cuts at: 0 1392 1722 2244
Size: 1392 330 522
Fragments arranged by size:
1392 522 330
SexAI A'CCwGG_T
Cuts at: 0 1660 2244
Size: 1660 584
SfcI C'TryA_G
Cuts at: 0 1369 2152 2244
Size: 1369 783 92
Fragments arranged by size:
1369 783 92
SmlI C'TyrA_G
Cuts at: 0 127 631 790 1336 2244
Size: 127 504 159 546 908
Fragments arranged by size:
908 546 504 159 127
StyI C'CwwG_G
Cuts at: 0 42 176 1061 1097 2244
Size: 42 134 885 36 1147
Fragments arranged by size:
1147 885 134 42 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 59 336 340 2244
Size: 59 277 4 1904
Fragments arranged by size:
1904 277 59 4
TatI w'GTAC_w
Cuts at: 0 609 819 1428 2244
Size: 609 210 609 816
Fragments arranged by size:
816 609 609 210
XbaI T'CTAG_A
Cuts at: 0 1237 2244
Size: 1237 1007
XmnI GAAnn'nnTTC
Cuts at: 0 260 2077 2244
Size: 260 1817 167
Fragments arranged by size:
1817 260 167
Enzymes that do cut:
AarI Acc65I AcuI AflII AleI AloI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BciVI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaBI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrDI BsrGI BstBI BstXI BstYI
Bsu36I BtgI BtsI DraIII EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI FspAI Hin4I HincII HpaI
KpnI MmeI MslI NcoI NdeI NspI PasI PfoI
PpiI PpuMI PspOMI PvuI SacI SapI SexAI SfcI
SmlI StyI TaqII TatI XbaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflIII AgeI AhdI AlfI
AscI AseI AsiSI AvrII BaeI BbeI BcgI BclI
BfrBI BglI BglII BmgBI BmtI BplI BsaAI BsaHI
BsaWI BsiWI BsmBI BspEI BsrBI BsrFI BssHII BssSI
BstAPI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI
EaeI EagI EcoRI EcoRV FseI HaeII HindIII KasI
MfeI MluI MscI MspA1I NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PshAI PsiI PspXI PsrI PstI PvuII RsrII SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XcmI XhoI XmaI ZraI