Restriction map for
pFN21AE4213
(Linear) (Six-base) MAPSORT of: KIEE4213.seq Check: 5962 from: 1 to: 2115
KIEE4213.orf
With 182 enzymes: SgfI *
July 3, 2012 18:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 589 2115
Size: 589 1526
AccI GT'mk_AC
Cuts at: 0 1361 2115
Size: 1361 754
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 311 1291 1882 2115
Size: 135 176 980 591 233
Fragments arranged by size:
980 591 233 176 135
AflIII A'CryG_T
Cuts at: 0 611 2115
Size: 611 1504
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1267 1299 2115
Size: 1267 32 816
Fragments arranged by size:
1267 816 32
AlwNI CAG_nnn'CTG
Cuts at: 0 146 1676 2115
Size: 146 1530 439
Fragments arranged by size:
1530 439 146
ApaI G_GGCC'C
Cuts at: 0 341 2115
Size: 341 1774
BamHI G'GATC_C
Cuts at: 0 1524 2115
Size: 1524 591
BanII G_rGCy'C
Cuts at: 0 341 2115
Size: 341 1774
BbsI GAAGACnn'nnnn_
Cuts at: 0 119 1779 2115
Size: 119 1660 336
Fragments arranged by size:
1660 336 119
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 391 395 425 429 2115
Size: 391 4 30 4 1686
Fragments arranged by size:
1686 391 30 4 4
BfrBI ATG'CAT
Cuts at: 0 461 831 2115
Size: 461 370 1284
Fragments arranged by size:
1284 461 370
BglI GCCn_nnn'nGGC
Cuts at: 0 272 2115
Size: 272 1843
BglII A'GATC_T
Cuts at: 0 797 1553 2115
Size: 797 756 562
Fragments arranged by size:
797 756 562
BlpI GC'TnA_GC
Cuts at: 0 1007 2115
Size: 1007 1108
Bme1580I G_kGCm'C
Cuts at: 0 162 341 1853 2115
Size: 162 179 1512 262
Fragments arranged by size:
1512 262 179 162
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1246 1445 2115
Size: 1246 199 670
Fragments arranged by size:
1246 670 199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 748 1402 2115
Size: 91 657 654 713
Fragments arranged by size:
713 657 654 91
Bpu10I CC'TnA_GC
Cuts at: 0 2048 2115
Size: 2048 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 700 1082 2060 2115
Size: 262 438 382 978 55
Fragments arranged by size:
978 438 382 262 55
BsaI GGTCTCn'nnnn_
Cuts at: 0 480 668 2115
Size: 480 188 1447
Fragments arranged by size:
1447 480 188
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1767 2115
Size: 1767 348
BseYI C'CCAG_C
Cuts at: 0 12 1917 2115
Size: 12 1905 198
Fragments arranged by size:
1905 198 12
BsiHKAI G_wGCw'C
Cuts at: 0 138 2115
Size: 138 1977
BsmI GAATG_Cn'
Cuts at: 0 1776 2065 2115
Size: 1776 289 50
Fragments arranged by size:
1776 289 50
Bsp1286I G_dGCh'C
Cuts at: 0 138 162 341 1853 2115
Size: 138 24 179 1512 262
Fragments arranged by size:
1512 262 179 138 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 227 241 589 798 942 2115
Size: 227 14 348 209 144 1173
Fragments arranged by size:
1173 348 227 209 144 14
BsrBI CCG'CTC
Cuts at: 0 1424 2052 2115
Size: 1424 628 63
Fragments arranged by size:
1424 628 63
BsrDI GCAATG_nn'
Cuts at: 0 1727 2115
Size: 1727 388
BsrFI r'CCGG_y
Cuts at: 0 1465 2115
Size: 1465 650
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1637 2115
Size: 1637 478
BstYI r'GATC_y
Cuts at: 0 797 1268 1524 1553 2115
Size: 797 471 256 29 562
Fragments arranged by size:
797 562 471 256 29
Bsu36I CC'TnA_GG
Cuts at: 0 36 2115
Size: 36 2079
BtgI C'CryG_G
Cuts at: 0 20 2115
Size: 20 2095
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 148 2115
Size: 148 1967
BtsI GCAGTG_nn'
Cuts at: 0 386 2115
Size: 386 1729
ClaI AT'CG_AT
Cuts at: 0 702 2115
Size: 702 1413
DraI TTT'AAA
Cuts at: 0 1518 2115
Size: 1518 597
DraIII CAC_nnn'GTG
Cuts at: 0 1637 2115
Size: 1637 478
DrdI GACnn_nn'nnGTC
Cuts at: 0 682 2115
Size: 682 1433
EaeI y'GGCC_r
Cuts at: 0 782 1326 2115
Size: 782 544 789
Fragments arranged by size:
789 782 544
EarI CTCTTCn'nnn_
Cuts at: 0 1597 2115
Size: 1597 518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 135 311 748 1291 1402 1882 2115
Size: 91 44 176 437 543 111 480 233
Fragments arranged by size:
543 480 437 233 176 111 91 44
EcoO109I rG'GnC_Cy
Cuts at: 0 264 432 2115
Size: 264 168 1683
Fragments arranged by size:
1683 264 168
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 136 2115
Size: 136 1979
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1576 1608 2115
Size: 1576 32 507
Fragments arranged by size:
1576 507 32
HincII GTy'rAC
Cuts at: 0 986 2115
Size: 986 1129
MfeI C'AATT_G
Cuts at: 0 634 1721 2115
Size: 634 1087 394
Fragments arranged by size:
1087 634 394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1999 2115
Size: 1999 116
MscI TGG'CCA
Cuts at: 0 784 1328 2115
Size: 784 544 787
Fragments arranged by size:
787 784 544
MslI CAynn'nnrTG
Cuts at: 0 391 596 2115
Size: 391 205 1519
Fragments arranged by size:
1519 391 205
MspA1I CmG'CkG
Cuts at: 0 155 1367 2115
Size: 155 1212 748
Fragments arranged by size:
1212 748 155
NaeI GCC'GGC
Cuts at: 0 1467 2115
Size: 1467 648
NgoMIV G'CCGG_C
Cuts at: 0 1465 2115
Size: 1465 650
NsiI A_TGCA'T
Cuts at: 0 463 833 2115
Size: 463 370 1282
Fragments arranged by size:
1282 463 370
NspI r_CATG'y
Cuts at: 0 461 615 2115
Size: 461 154 1500
Fragments arranged by size:
1500 461 154
PciI A'CATG_T
Cuts at: 0 611 2115
Size: 611 1504
PflMI CCAn_nnn'nTGG
Cuts at: 0 540 2115
Size: 540 1575
PfoI T'CCnGG_A
Cuts at: 0 1880 2115
Size: 1880 235
PpuMI rG'GwC_Cy
Cuts at: 0 432 2115
Size: 432 1683
PspOMI G'GGCC_C
Cuts at: 0 337 2115
Size: 337 1778
PstI C_TGCA'G
Cuts at: 0 187 238 299 2071 2115
Size: 187 51 61 1772 44
Fragments arranged by size:
1772 187 61 51 44
PvuII CAG'CTG
Cuts at: 0 155 1367 2115
Size: 155 1212 748
Fragments arranged by size:
1212 748 155
SapI GCTCTTCn'nnn_
Cuts at: 0 1597 2115
Size: 1597 518
SbfI CC_TGCA'GG
Cuts at: 0 238 2115
Size: 238 1877
SexAI A'CCwGG_T
Cuts at: 0 746 2115
Size: 746 1369
SfcI C'TryA_G
Cuts at: 0 183 234 295 1045 2067 2115
Size: 183 51 61 750 1022 48
Fragments arranged by size:
1022 750 183 61 51 48
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 272 2115
Size: 272 1843
SmlI C'TyrA_G
Cuts at: 0 277 715 1097 2075 2115
Size: 277 438 382 978 40
Fragments arranged by size:
978 438 382 277 40
SspI AAT'ATT
Cuts at: 0 1062 2115
Size: 1062 1053
StuI AGG'CCT
Cuts at: 0 2073 2115
Size: 2073 42
StyI C'CwwG_G
Cuts at: 0 439 2115
Size: 439 1676
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1861 2115
Size: 1861 254
TatI w'GTAC_w
Cuts at: 0 614 1234 1310 2115
Size: 614 620 76 805
Fragments arranged by size:
805 620 614 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1202 1234 2115
Size: 1202 32 881
Fragments arranged by size:
1202 881 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 537 1754 2115
Size: 537 1217 361
Fragments arranged by size:
1217 537 361
XmnI GAAnn'nnTTC
Cuts at: 0 523 2115
Size: 523 1592
Enzymes that do cut:
AarI AccI AcuI AflIII AloI AlwNI ApaI BamHI
BanII BbsI BcgI BfrBI BglI BglII BlpI Bme1580I
BplI BpmI Bpu10I BpuEI BsaI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EarI Eco57MI EcoO109I FalI Hin4I HincII MfeI
MmeI MscI MslI MspA1I NaeI NgoMIV NsiI NspI
PciI PflMI PfoI PpuMI PspOMI PstI PvuII SapI
SbfI SexAI SfcI SfiI SmlI SspI StuI StyI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BanI BbeI BbvCI BciVI BclI BmgBI BmrI
BmtI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I CspCI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MluI NarI NcoI NdeI NheI NotI
NruI PacI PasI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
ScaI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI Tth111I XbaI XhoI XmaI ZraI