Restriction map for
pFN21AE4212
(Linear) (Six-base) MAPSORT of: KIEE4212.seq Check: 5031 from: 1 to: 2088
KIEE4212.orf
With 182 enzymes: SgfI *
July 3, 2012 18:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 2088
Size: 683 1405
AhdI GACnn_n'nnGTC
Cuts at: 0 21 2053 2088
Size: 21 2032 35
Fragments arranged by size:
2032 35 21
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 175 207 2088
Size: 175 32 1881
Fragments arranged by size:
1881 175 32
ApaI G_GGCC'C
Cuts at: 0 285 669 2088
Size: 285 384 1419
Fragments arranged by size:
1419 384 285
ApaLI G'TGCA_C
Cuts at: 0 2000 2088
Size: 2000 88
ApoI r'AATT_y
Cuts at: 0 829 929 1086 1267 1535 2088
Size: 829 100 157 181 268 553
Fragments arranged by size:
829 553 268 181 157 100
AvaI C'yCGr_G
Cuts at: 0 143 225 382 2088
Size: 143 82 157 1706
Fragments arranged by size:
1706 157 143 82
BamHI G'GATC_C
Cuts at: 0 1791 2088
Size: 1791 297
BanI G'GyrC_C
Cuts at: 0 86 92 366 506 2088
Size: 86 6 274 140 1582
Fragments arranged by size:
1582 274 140 86 6
BanII G_rGCy'C
Cuts at: 0 7 285 669 1317 1995 2011 2088
Size: 7 278 384 648 678 16 77
Fragments arranged by size:
678 648 384 278 77 16 7
BbeI G_GCGC'C
Cuts at: 0 90 96 2088
Size: 90 6 1992
Fragments arranged by size:
1992 90 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 105 2088
Size: 105 1983
BbvCI CC'TCA_GC
Cuts at: 0 295 874 2088
Size: 295 579 1214
Fragments arranged by size:
1214 579 295
BglI GCCn_nnn'nGGC
Cuts at: 0 77 884 2088
Size: 77 807 1204
Fragments arranged by size:
1204 807 77
BglII A'GATC_T
Cuts at: 0 854 2088
Size: 854 1234
Bme1580I G_kGCm'C
Cuts at: 0 285 371 511 669 949 1202 1459 2004
Size: 285 86 140 158 280 253 257 545
Cuts at: 2004 2019 2088
Size: 15 69
Fragments arranged by size:
545 285 280 257 253 158 140 86
69 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 1126 2088
Size: 1126 962
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 33 1965 2088
Size: 33 1932 123
Fragments arranged by size:
1932 123 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 947 1121 1329 1464 2088
Size: 367 580 174 208 135 624
Fragments arranged by size:
624 580 367 208 174 135
Bpu10I CC'TnA_GC
Cuts at: 0 275 295 874 2088
Size: 275 20 579 1214
Fragments arranged by size:
1214 579 275 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1303 2088
Size: 1303 785
BsaI GGTCTCn'nnnn_
Cuts at: 0 709 2088
Size: 709 1379
BsaHI Gr'CG_yC
Cuts at: 0 87 93 2088
Size: 87 6 1995
Fragments arranged by size:
1995 87 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 175 205 229 259 2088
Size: 175 30 24 30 1829
Fragments arranged by size:
1829 175 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 217 250 283 472 1118 2088
Size: 217 33 33 189 646 970
Fragments arranged by size:
970 646 217 189 33 33
BseYI C'CCAG_C
Cuts at: 0 942 1327 1865 2088
Size: 942 385 538 223
Fragments arranged by size:
942 538 385 223
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 408 833 2088
Size: 408 425 1255
Fragments arranged by size:
1255 425 408
BsiEI CG_ry'CG
Cuts at: 0 53 84 135 2088
Size: 53 31 51 1953
Fragments arranged by size:
1953 53 51 31
BsiHKAI G_wGCw'C
Cuts at: 0 963 1375 2004 2088
Size: 963 412 629 84
Fragments arranged by size:
963 629 412 84
BsmI GAATG_Cn'
Cuts at: 0 1016 1472 1645 2088
Size: 1016 456 173 443
Fragments arranged by size:
1016 456 443 173
Bsp1286I G_dGCh'C
Cuts at: 0 7 285 371 511 669 949 963 1202
Size: 7 278 86 140 158 280 14 239
Cuts at: 1202 1317 1375 1459 1995 2004 2011 2019 2088
Size: 115 58 84 536 9 7 8 69
Fragments arranged by size:
536 280 278 239 158 140 115 86
84 69 58 14 9 8 7 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 347 1040 1493 2088
Size: 347 693 453 595
Fragments arranged by size:
693 595 453 347
BsrBI CCG'CTC
Cuts at: 0 279 311 1958 2088
Size: 279 32 1647 130
Fragments arranged by size:
1647 279 130 32
BsrDI GCAATG_nn'
Cuts at: 0 980 989 1121 2088
Size: 980 9 132 967
Fragments arranged by size:
980 967 132 9
BsrFI r'CCGG_y
Cuts at: 0 83 89 1600 2088
Size: 83 6 1511 488
Fragments arranged by size:
1511 488 83 6
BsrGI T'GTAC_A
Cuts at: 0 889 2088
Size: 889 1199
BssSI C'ACGA_G
Cuts at: 0 312 1589 2088
Size: 312 1277 499
Fragments arranged by size:
1277 499 312
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1176 2088
Size: 1176 912
BstYI r'GATC_y
Cuts at: 0 854 1791 2088
Size: 854 937 297
Fragments arranged by size:
937 854 297
BtgI C'CryG_G
Cuts at: 0 129 910 1004 2088
Size: 129 781 94 1084
Fragments arranged by size:
1084 781 129 94
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 23 2088
Size: 23 2065
BtsI GCAGTG_nn'
Cuts at: 0 609 768 821 1788 1890 2088
Size: 609 159 53 967 102 198
Fragments arranged by size:
967 609 198 159 102 53
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1277 1312 2088
Size: 1277 35 776
Fragments arranged by size:
1277 776 35
EaeI y'GGCC_r
Cuts at: 0 50 81 132 1430 1598 1835 2088
Size: 50 31 51 1298 168 237 253
Fragments arranged by size:
1298 253 237 168 51 50 31
EagI C'GGCC_G
Cuts at: 0 50 81 132 2088
Size: 50 31 51 1956
Fragments arranged by size:
1956 51 50 31
EarI CTCTTCn'nnn_
Cuts at: 0 1781 2088
Size: 1781 307
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 196 226 319 1790 2088
Size: 196 30 93 1471 298
Fragments arranged by size:
1471 298 196 93 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 683 947 1121 1329 1464 2088
Size: 367 316 264 174 208 135 624
Fragments arranged by size:
624 367 316 264 208 174 135
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2041 2088
Size: 2041 47
EcoO109I rG'GnC_Cy
Cuts at: 0 282 665 1414 2088
Size: 282 383 749 674
Fragments arranged by size:
749 674 383 282
EcoRI G'AATT_C
Cuts at: 0 829 2088
Size: 829 1259
HaeII r_GCGC'y
Cuts at: 0 90 96 2088
Size: 90 6 1992
Fragments arranged by size:
1992 90 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 612 644 945 977 1894 1918
Size: 7 32 573 32 301 32 917 24
Cuts at: 1918 1926 1950 2039 2071 2088
Size: 8 24 89 32 17
Fragments arranged by size:
917 573 301 89 32 32 32 32
24 24 17 8 7
HincII GTy'rAC
Cuts at: 0 658 2088
Size: 658 1430
KasI G'GCGC_C
Cuts at: 0 86 92 2088
Size: 86 6 1996
Fragments arranged by size:
1996 86 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1011 2088
Size: 1011 1077
MscI TGG'CCA
Cuts at: 0 1837 2088
Size: 1837 251
MslI CAynn'nnrTG
Cuts at: 0 1733 2088
Size: 1733 355
MspA1I CmG'CkG
Cuts at: 0 77 131 252 299 377 503 557 942
Size: 77 54 121 47 78 126 54 385
Cuts at: 942 1695 2088
Size: 753 393
Fragments arranged by size:
753 393 385 126 121 78 77 54
54 47
NaeI GCC'GGC
Cuts at: 0 85 91 2088
Size: 85 6 1997
Fragments arranged by size:
1997 85 6
NarI GG'CG_CC
Cuts at: 0 87 93 2088
Size: 87 6 1995
Fragments arranged by size:
1995 87 6
NcoI C'CATG_G
Cuts at: 0 910 1004 2088
Size: 910 94 1084
Fragments arranged by size:
1084 910 94
NdeI CA'TA_TG
Cuts at: 0 1182 2021 2088
Size: 1182 839 67
Fragments arranged by size:
1182 839 67
NgoMIV G'CCGG_C
Cuts at: 0 83 89 2088
Size: 83 6 1999
Fragments arranged by size:
1999 83 6
NotI GC'GGCC_GC
Cuts at: 0 50 132 2088
Size: 50 82 1956
Fragments arranged by size:
1956 82 50
NspI r_CATG'y
Cuts at: 0 49 644 2088
Size: 49 595 1444
Fragments arranged by size:
1444 595 49
PflMI CCAn_nnn'nTGG
Cuts at: 0 2071 2088
Size: 2071 17
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 175 207 2088
Size: 175 32 1881
Fragments arranged by size:
1881 175 32
PpuMI rG'GwC_Cy
Cuts at: 0 1414 2088
Size: 1414 674
PspOMI G'GGCC_C
Cuts at: 0 281 665 2088
Size: 281 384 1423
Fragments arranged by size:
1423 384 281
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 445 477 2088
Size: 445 32 1611
Fragments arranged by size:
1611 445 32
PstI C_TGCA'G
Cuts at: 0 1146 1804 1971 2088
Size: 1146 658 167 117
Fragments arranged by size:
1146 658 167 117
PvuII CAG'CTG
Cuts at: 0 299 377 557 942 1695 2088
Size: 299 78 180 385 753 393
Fragments arranged by size:
753 393 385 299 180 78
SacII CC_GC'GG
Cuts at: 0 132 2088
Size: 132 1956
SfcI C'TryA_G
Cuts at: 0 1142 1388 1800 1967 2088
Size: 1142 246 412 167 121
Fragments arranged by size:
1142 412 246 167 121
SfoI GGC'GCC
Cuts at: 0 88 94 2088
Size: 88 6 1994
Fragments arranged by size:
1994 88 6
SgrAI Cr'CCGG_yG
Cuts at: 0 89 2088
Size: 89 1999
SmaI CCC'GGG
Cuts at: 0 145 227 2088
Size: 145 82 1861
Fragments arranged by size:
1861 145 82
SmlI C'TyrA_G
Cuts at: 0 1318 2088
Size: 1318 770
SpeI A'CTAG_T
Cuts at: 0 1360 1375 2088
Size: 1360 15 713
Fragments arranged by size:
1360 713 15
SrfI GCCC'GGGC
Cuts at: 0 145 227 2088
Size: 145 82 1861
Fragments arranged by size:
1861 145 82
StuI AGG'CCT
Cuts at: 0 1355 2088
Size: 1355 733
StyI C'CwwG_G
Cuts at: 0 910 1004 1846 1995 2088
Size: 910 94 842 149 93
Fragments arranged by size:
910 842 149 94 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1107 2088
Size: 1107 981
TatI w'GTAC_w
Cuts at: 0 889 2088
Size: 889 1199
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 229 261 1241 1273 2088
Size: 229 32 980 32 815
Fragments arranged by size:
980 815 229 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1113 2088
Size: 1113 975
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1002 2088
Size: 1002 1086
XmaI C'CCGG_G
Cuts at: 0 143 225 2088
Size: 143 82 1863
Fragments arranged by size:
1863 143 82
Enzymes that do cut:
AcuI AhdI AloI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BglI BglII Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstXI BstYI BtgI
BtgZI BtsI CspCI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NotI NspI PflMI PpiI PpuMI PspOMI PsrI PstI
PvuII SacII SfcI SfoI SgrAI SmaI SmlI SpeI
SrfI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AlwNI AscI AseI AsiSI AvrII
BaeI BcgI BciVI BclI BfrBI BlpI BmgBI BmtI
BsaAI BsaBI BsaWI BsiWI BsmBI BspEI BspHI BssHII
BstAPI BstBI BstEII BstZ17I Bsu36I ClaI DraI DraIII
DrdI EcoICRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI NheI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PshAI PsiI PspXI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SnaBI SphI SspI SwaI XbaI
XhoI XmnI ZraI