Restriction map for
pFN21AE4209
(Linear) (Six-base) MAPSORT of: KIEE4209.seq Check: 9156 from: 1 to: 2187
KIEE4209.orf
With 182 enzymes: SgfI *
July 3, 2012 18:46 ..
AatII G_ACGT'C
Cuts at: 0 324 2187
Size: 324 1863
AccI GT'mk_AC
Cuts at: 0 108 847 2187
Size: 108 739 1340
Fragments arranged by size:
1340 739 108
Acc65I G'GTAC_C
Cuts at: 0 485 2187
Size: 485 1702
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 2048 2187
Size: 929 1119 139
Fragments arranged by size:
1119 929 139
AflIII A'CryG_T
Cuts at: 0 842 1148 2187
Size: 842 306 1039
Fragments arranged by size:
1039 842 306
AleI CACnn'nnGTG
Cuts at: 0 976 1105 1597 2075 2187
Size: 976 129 492 478 112
Fragments arranged by size:
976 492 478 129 112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 994 2187
Size: 994 1193
ApaI G_GGCC'C
Cuts at: 0 582 865 2047 2187
Size: 582 283 1182 140
Fragments arranged by size:
1182 582 283 140
AvaI C'yCGr_G
Cuts at: 0 383 2187
Size: 383 1804
BamHI G'GATC_C
Cuts at: 0 1558 1586 1673 1775 2187
Size: 1558 28 87 102 412
Fragments arranged by size:
1558 412 102 87 28
BanI G'GyrC_C
Cuts at: 0 143 378 485 882 950 2187
Size: 143 235 107 397 68 1237
Fragments arranged by size:
1237 397 235 143 107 68
BanII G_rGCy'C
Cuts at: 0 244 259 582 865 1354 1739 2047 2187
Size: 244 15 323 283 489 385 308 140
Fragments arranged by size:
489 385 323 308 283 244 140 15
BbeI G_GCGC'C
Cuts at: 0 954 2187
Size: 954 1233
BbsI GAAGACnn'nnnn_
Cuts at: 0 1814 2187
Size: 1814 373
BbvCI CC'TCA_GC
Cuts at: 0 1643 1689 2187
Size: 1643 46 498
Fragments arranged by size:
1643 498 46
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 267 301 2063 2097 2187
Size: 267 34 1762 34 90
Fragments arranged by size:
1762 267 90 34 34
BclI T'GATC_A
Cuts at: 0 665 1007 2187
Size: 665 342 1180
Fragments arranged by size:
1180 665 342
BglI GCCn_nnn'nGGC
Cuts at: 0 1244 2187
Size: 1244 943
Bme1580I G_kGCm'C
Cuts at: 0 37 148 582 865 1463 2047 2187
Size: 37 111 434 283 598 584 140
Fragments arranged by size:
598 584 434 283 140 111 37
BmgBI CAC'GTC
Cuts at: 0 845 2187
Size: 845 1342
BmrI ACTGGGnnnn_n'
Cuts at: 0 696 970 2187
Size: 696 274 1217
Fragments arranged by size:
1217 696 274
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1853 2187
Size: 1853 334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 816 975 1073 1561 2187
Size: 49 767 159 98 488 626
Fragments arranged by size:
767 626 488 159 98 49
Bpu10I CC'TnA_GC
Cuts at: 0 231 1252 1643 1689 2187
Size: 231 1021 391 46 498
Fragments arranged by size:
1021 498 391 231 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 1878 1996 2187
Size: 628 1250 118 191
Fragments arranged by size:
1250 628 191 118
BsaI GGTCTCn'nnnn_
Cuts at: 0 1363 1526 1563 2187
Size: 1363 163 37 624
Fragments arranged by size:
1363 624 163 37
BsaAI yAC'GTr
Cuts at: 0 1748 2187
Size: 1748 439
BsaHI Gr'CG_yC
Cuts at: 0 160 321 951 1217 1259 2017 2187
Size: 160 161 630 266 42 758 170
Fragments arranged by size:
758 630 266 170 161 160 42
BsaWI w'CCGG_w
Cuts at: 0 461 926 1984 2089 2108 2187
Size: 461 465 1058 105 19 79
Fragments arranged by size:
1058 465 461 105 79 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 698 728 1202 1232 1341 1371 1696 1726
Size: 698 30 474 30 109 30 325 30
Cuts at: 1726 2187
Size: 461
Fragments arranged by size:
698 474 461 325 109 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1377 1671 1940 2187
Size: 1377 294 269 247
Fragments arranged by size:
1377 294 269 247
BseYI C'CCAG_C
Cuts at: 0 522 557 1013 1127 2021 2179 2187
Size: 522 35 456 114 894 158 8
Fragments arranged by size:
894 522 456 158 114 35 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 783 1147 2187
Size: 783 364 1040
Fragments arranged by size:
1040 783 364
BsiHKAI G_wGCw'C
Cuts at: 0 244 983 2187
Size: 244 739 1204
Fragments arranged by size:
1204 739 244
BsmI GAATG_Cn'
Cuts at: 0 157 2187
Size: 157 2030
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1266 2187
Size: 1266 921
Bsp1286I G_dGCh'C
Cuts at: 0 37 148 244 259 582 865 983 1354
Size: 37 111 96 15 323 283 118 371
Cuts at: 1354 1463 1739 2047 2187
Size: 109 276 308 140
Fragments arranged by size:
371 323 308 283 276 140 118 111
109 96 37 15
BspEI T'CCGG_A
Cuts at: 0 461 1984 2089 2108 2187
Size: 461 1523 105 19 79
Fragments arranged by size:
1523 461 105 79 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 206 1208 2187
Size: 206 1002 979
Fragments arranged by size:
1002 979 206
BsrBI CCG'CTC
Cuts at: 0 570 2187
Size: 570 1617
BsrFI r'CCGG_y
Cuts at: 0 189 1495 2187
Size: 189 1306 692
Fragments arranged by size:
1306 692 189
BssSI C'ACGA_G
Cuts at: 0 205 1597 2187
Size: 205 1392 590
Fragments arranged by size:
1392 590 205
BstEII G'GTnAC_C
Cuts at: 0 424 1269 2187
Size: 424 845 918
Fragments arranged by size:
918 845 424
BstXI CCAn_nnnn'nTGG
Cuts at: 0 373 534 1050 1107 1599 2077 2142 2187
Size: 373 161 516 57 492 478 65 45
Fragments arranged by size:
516 492 478 373 161 65 57 45
BstYI r'GATC_y
Cuts at: 0 1558 1586 1673 1775 2187
Size: 1558 28 87 102 412
Fragments arranged by size:
1558 412 102 87 28
Bsu36I CC'TnA_GG
Cuts at: 0 430 2157 2187
Size: 430 1727 30
Fragments arranged by size:
1727 430 30
BtgI C'CryG_G
Cuts at: 0 533 1019 1106 1179 1426 1628 1694 1784
Size: 533 486 87 73 247 202 66 90
Cuts at: 1784 2187
Size: 403
Fragments arranged by size:
533 486 403 247 202 90 87 73
66
BtsI GCAGTG_nn'
Cuts at: 0 1463 1770 2150 2187
Size: 1463 307 380 37
Fragments arranged by size:
1463 380 307 37
EaeI y'GGCC_r
Cuts at: 0 191 1787 2187
Size: 191 1596 400
Fragments arranged by size:
1596 400 191
EarI CTCTTCn'nnn_
Cuts at: 0 1529 2187
Size: 1529 658
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1551 2187
Size: 1551 636
EcoICRI GAG'CTC
Cuts at: 0 242 2187
Size: 242 1945
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 816 929 975 1073 1561 2048 2187
Size: 49 767 113 46 98 488 487 139
Fragments arranged by size:
767 488 487 139 113 98 49 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 264 1204 2187
Size: 264 940 983
Fragments arranged by size:
983 940 264
EcoO109I rG'GnC_Cy
Cuts at: 0 578 579 861 1224 2187
Size: 578 1 282 363 963
Fragments arranged by size:
963 578 363 282 1
EcoRV GAT'ATC
Cuts at: 0 450 654 2187
Size: 450 204 1533
Fragments arranged by size:
1533 450 204
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1540 2187
Size: 1540 647
HaeII r_GCGC'y
Cuts at: 0 187 954 2187
Size: 187 767 1233
Fragments arranged by size:
1233 767 187
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 437 469 1202 1234 1271 1303 1341 1373
Size: 437 32 733 32 37 32 38 32
Cuts at: 1373 1578 1610 1696 1728 2187
Size: 205 32 86 32 459
Fragments arranged by size:
733 459 437 205 86 38 37 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1476 2187
Size: 1476 711
KasI G'GCGC_C
Cuts at: 0 950 2187
Size: 950 1237
KpnI G_GTAC'C
Cuts at: 0 489 2187
Size: 489 1698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 2187
Size: 197 1990
MslI CAynn'nnrTG
Cuts at: 0 371 661 976 1105 1597 1876 2075 2187
Size: 371 290 315 129 492 279 199 112
Fragments arranged by size:
492 371 315 290 279 199 129 112
MspA1I CmG'CkG
Cuts at: 0 512 552 1046 1786 2187
Size: 512 40 494 740 401
Fragments arranged by size:
740 512 494 401 40
NaeI GCC'GGC
Cuts at: 0 191 1497 2187
Size: 191 1306 690
Fragments arranged by size:
1306 690 191
NarI GG'CG_CC
Cuts at: 0 951 2187
Size: 951 1236
NcoI C'CATG_G
Cuts at: 0 1019 1179 1628 2187
Size: 1019 160 449 559
Fragments arranged by size:
1019 559 449 160
NdeI CA'TA_TG
Cuts at: 0 956 2187
Size: 956 1231
NgoMIV G'CCGG_C
Cuts at: 0 189 1495 2187
Size: 189 1306 692
Fragments arranged by size:
1306 692 189
NspI r_CATG'y
Cuts at: 0 1152 2187
Size: 1152 1035
PasI CC'CwG_GG
Cuts at: 0 865 1501 2187
Size: 865 636 686
Fragments arranged by size:
865 686 636
PciI A'CATG_T
Cuts at: 0 1148 2187
Size: 1148 1039
PflMI CCAn_nnn'nTGG
Cuts at: 0 1020 2187
Size: 1020 1167
PfoI T'CCnGG_A
Cuts at: 0 244 871 1089 1345 1582 2187
Size: 244 627 218 256 237 605
Fragments arranged by size:
627 605 256 244 237 218
PmlI CAC'GTG
Cuts at: 0 1748 2187
Size: 1748 439
PpuMI rG'GwC_Cy
Cuts at: 0 1224 2187
Size: 1224 963
PshAI GACnn'nnGTC
Cuts at: 0 1518 2187
Size: 1518 669
PspOMI G'GGCC_C
Cuts at: 0 578 861 2043 2187
Size: 578 283 1182 144
Fragments arranged by size:
1182 578 283 144
PspXI vC'TCGA_Gb
Cuts at: 0 383 2187
Size: 383 1804
PstI C_TGCA'G
Cuts at: 0 329 577 1568 2187
Size: 329 248 991 619
Fragments arranged by size:
991 619 329 248
PvuII CAG'CTG
Cuts at: 0 552 1046 2187
Size: 552 494 1141
Fragments arranged by size:
1141 552 494
SacI G_AGCT'C
Cuts at: 0 244 2187
Size: 244 1943
SacII CC_GC'GG
Cuts at: 0 1787 2187
Size: 1787 400
SfcI C'TryA_G
Cuts at: 0 109 325 405 573 1564 2187
Size: 109 216 80 168 991 623
Fragments arranged by size:
991 623 216 168 109 80
SfoI GGC'GCC
Cuts at: 0 952 2187
Size: 952 1235
SmlI C'TyrA_G
Cuts at: 0 383 643 1893 2011 2187
Size: 383 260 1250 118 176
Fragments arranged by size:
1250 383 260 176 118
StuI AGG'CCT
Cuts at: 0 270 394 2187
Size: 270 124 1793
Fragments arranged by size:
1793 270 124
StyI C'CwwG_G
Cuts at: 0 1019 1179 1265 1401 1628 2039 2129 2187
Size: 1019 160 86 136 227 411 90 58
Fragments arranged by size:
1019 411 227 160 136 90 86 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1027 2187
Size: 1027 1160
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1658 1690 1856 1888 1959 1991 2187
Size: 1658 32 166 32 71 32 196
Fragments arranged by size:
1658 196 166 71 32 32 32
XhoI C'TCGA_G
Cuts at: 0 383 2187
Size: 383 1804
ZraI GAC'GTC
Cuts at: 0 322 2187
Size: 322 1865
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AleI AlfI ApaI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BclI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrFI BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FalI HaeII Hin4I HincII KasI KpnI MmeI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI SacII SfcI SfoI SmlI StuI
StyI TaqII TstI XhoI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI AloI AlwNI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BciVI
BfrBI BglII BlpI BmtI BsaBI BsiEI BsiWI BspHI
BsrDI BsrGI BssHII BstAPI BstBI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EcoRI FseI FspI
FspAI HindIII HpaI MfeI MluI MscI NheI NotI
NruI NsiI PacI PmeI PpiI PsiI PsrI PvuI
RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TatI Tth111I XbaI XcmI XmaI XmnI