Restriction map for
pFN21AE4208
(Linear) (Six-base) MAPSORT of: KIEE4208.seq Check: 2086 from: 1 to: 2184
KIEE4208.orf
With 182 enzymes: SgfI *
July 3, 2012 18:45 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 597 2184
Size: 597 1587
AflIII A'CryG_T
Cuts at: 0 662 2184
Size: 662 1522
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 549 1270 2184
Size: 549 721 914
Fragments arranged by size:
914 721 549
ApoI r'AATT_y
Cuts at: 0 25 551 991 1924 2031 2129 2184
Size: 25 526 440 933 107 98 55
Fragments arranged by size:
933 526 440 107 98 55 25
AseI AT'TA_AT
Cuts at: 0 2113 2184
Size: 2113 71
AvaI C'yCGr_G
Cuts at: 0 321 1811 2184
Size: 321 1490 373
Fragments arranged by size:
1490 373 321
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1428 1461 2184
Size: 1428 33 723
Fragments arranged by size:
1428 723 33
BanI G'GyrC_C
Cuts at: 0 1310 2184
Size: 1310 874
BanII G_rGCy'C
Cuts at: 0 92 2184
Size: 92 2092
BbsI GAAGACnn'nnnn_
Cuts at: 0 1187 2184
Size: 1187 997
BciVI GTATCCnnnnn_n'
Cuts at: 0 2108 2184
Size: 2108 76
Bme1580I G_kGCm'C
Cuts at: 0 637 2184
Size: 637 1547
BmgBI CAC'GTC
Cuts at: 0 1254 2184
Size: 1254 930
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1508 1514 2184
Size: 1508 6 670
Fragments arranged by size:
1508 670 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 1040 2184
Size: 476 564 1144
Fragments arranged by size:
1144 564 476
BsaI GGTCTCn'nnnn_
Cuts at: 0 1595 2184
Size: 1595 589
BsaBI GATnn'nnATC
Cuts at: 0 365 2045 2184
Size: 365 1680 139
Fragments arranged by size:
1680 365 139
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 236 266 2184
Size: 236 30 1918
Fragments arranged by size:
1918 236 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 957 1511 2184
Size: 957 554 673
Fragments arranged by size:
957 673 554
BseYI C'CCAG_C
Cuts at: 0 99 2184
Size: 99 2085
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1114 2184
Size: 1114 1070
BsiHKAI G_wGCw'C
Cuts at: 0 92 2184
Size: 92 2092
BsmI GAATG_Cn'
Cuts at: 0 547 2184
Size: 547 1637
Bsp1286I G_dGCh'C
Cuts at: 0 92 637 2184
Size: 92 545 1547
Fragments arranged by size:
1547 545 92
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1167 2184
Size: 1167 1017
BsrDI GCAATG_nn'
Cuts at: 0 543 2184
Size: 543 1641
BstAPI GCAn_nnn'nTGC
Cuts at: 0 542 2184
Size: 542 1642
BstBI TT'CG_AA
Cuts at: 0 399 2184
Size: 399 1785
BstEII G'GTnAC_C
Cuts at: 0 63 2184
Size: 63 2121
BstYI r'GATC_y
Cuts at: 0 366 2184
Size: 366 1818
BtsI GCAGTG_nn'
Cuts at: 0 756 2127 2184
Size: 756 1371 57
Fragments arranged by size:
1371 756 57
DrdI GACnn_nn'nnGTC
Cuts at: 0 1080 2184
Size: 1080 1104
EarI CTCTTCn'nnn_
Cuts at: 0 817 926 1004 1141 1591 2184
Size: 817 109 78 137 450 593
Fragments arranged by size:
817 593 450 137 109 78
EcoICRI GAG'CTC
Cuts at: 0 90 2184
Size: 90 2094
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 597 1508 1514 2184
Size: 597 911 6 670
Fragments arranged by size:
911 670 597 6
EcoRI G'AATT_C
Cuts at: 0 2129 2184
Size: 2129 55
EcoRV GAT'ATC
Cuts at: 0 433 2184
Size: 433 1751
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 199 231 236 268 925 957 1366 1398
Size: 199 32 5 32 657 32 409 32
Cuts at: 1398 2184
Size: 786
Fragments arranged by size:
786 657 409 199 32 32 32 32
5
HindIII A'AGCT_T
Cuts at: 0 775 1674 2184
Size: 775 899 510
Fragments arranged by size:
899 775 510
MfeI C'AATT_G
Cuts at: 0 959 2184
Size: 959 1225
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 2184
Size: 625 1559
MslI CAynn'nnrTG
Cuts at: 0 164 590 1252 1657 1856 2184
Size: 164 426 662 405 199 328
Fragments arranged by size:
662 426 405 328 199 164
NspI r_CATG'y
Cuts at: 0 107 666 1920 2184
Size: 107 559 1254 264
Fragments arranged by size:
1254 559 264 107
PciI A'CATG_T
Cuts at: 0 662 2184
Size: 662 1522
PsiI TTA'TAA
Cuts at: 0 1289 2184
Size: 1289 895
PstI C_TGCA'G
Cuts at: 0 1164 1647 2184
Size: 1164 483 537
Fragments arranged by size:
1164 537 483
SacI G_AGCT'C
Cuts at: 0 92 2184
Size: 92 2092
SapI GCTCTTCn'nnn_
Cuts at: 0 1141 2184
Size: 1141 1043
SexAI A'CCwGG_T
Cuts at: 0 1754 2184
Size: 1754 430
SfcI C'TryA_G
Cuts at: 0 519 1160 1485 1643 1910 2184
Size: 519 641 325 158 267 274
Fragments arranged by size:
641 519 325 274 267 158
SmaI CCC'GGG
Cuts at: 0 1813 2184
Size: 1813 371
SmlI C'TyrA_G
Cuts at: 0 491 1055 2184
Size: 491 564 1129
Fragments arranged by size:
1129 564 491
SphI G_CATG'C
Cuts at: 0 107 1920 2184
Size: 107 1813 264
Fragments arranged by size:
1813 264 107
StyI C'CwwG_G
Cuts at: 0 562 2184
Size: 562 1622
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 140 296 700 2184
Size: 140 156 404 1484
Fragments arranged by size:
1484 404 156 140
TatI w'GTAC_w
Cuts at: 0 665 2184
Size: 665 1519
XbaI T'CTAG_A
Cuts at: 0 1026 2184
Size: 1026 1158
XmaI C'CCGG_G
Cuts at: 0 1811 2184
Size: 1811 373
XmnI GAAnn'nnTTC
Cuts at: 0 1508 2184
Size: 1508 676
Enzymes that do cut:
AcuI AflIII AlfI ApoI AseI AvaI BaeI BanI
BanII BbsI BciVI Bme1580I BmgBI BpmI BpuEI BsaI
BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BstAPI BstBI BstEII BstYI BtsI DrdI
EarI EcoICRI Eco57MI EcoRI EcoRV Hin4I HindIII MfeI
MmeI MslI NspI PciI PsiI PstI SacI SapI
SexAI SfcI SmaI SmlI SphI StyI TaqII TatI
XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AsiSI
AvrII BamHI BbeI BbvCI BcgI BclI BfrBI BglI
BglII BlpI BmrI BmtI BplI Bpu10I BsaAI BsaHI
BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII EaeI EagI EciI EcoNI
EcoO109I FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MluI MscI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI StuI SwaI TstI Tth111I XcmI XhoI
ZraI