Restriction map for
pFN21AE4203
(Linear) (Six-base) MAPSORT of: KIEE4203.seq Check: 6361 from: 1 to: 2130
KIEE4203.orf
With 182 enzymes: SgfI *
March 22, 2012 17:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 720 2130
Size: 659 61 1410
Fragments arranged by size:
1410 659 61
AfeI AGC'GCT
Cuts at: 0 997 2130
Size: 997 1133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1762 2130
Size: 1762 368
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1006 1038 2130
Size: 1006 32 1092
Fragments arranged by size:
1092 1006 32
AlwNI CAG_nnn'CTG
Cuts at: 0 729 969 1258 1392 2130
Size: 729 240 289 134 738
Fragments arranged by size:
738 729 289 240 134
ApaI G_GGCC'C
Cuts at: 0 133 1727 1735 2130
Size: 133 1594 8 395
Fragments arranged by size:
1594 395 133 8
AvaI C'yCGr_G
Cuts at: 0 133 367 1496 1589 1621 2130
Size: 133 234 1129 93 32 509
Fragments arranged by size:
1129 509 234 133 93 32
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 293 326 2130
Size: 293 33 1804
Fragments arranged by size:
1804 293 33
BamHI G'GATC_C
Cuts at: 0 980 1201 2130
Size: 980 221 929
Fragments arranged by size:
980 929 221
BanI G'GyrC_C
Cuts at: 0 33 856 1273 2130
Size: 33 823 417 857
Fragments arranged by size:
857 823 417 33
BanII G_rGCy'C
Cuts at: 0 121 133 448 707 785 820 1426 1727
Size: 121 12 315 259 78 35 606 301
Cuts at: 1727 1735 1834 2130
Size: 8 99 296
Fragments arranged by size:
606 315 301 296 259 121 99 78
35 12 8
BbeI G_GCGC'C
Cuts at: 0 860 2130
Size: 860 1270
BbsI GAAGACnn'nnnn_
Cuts at: 0 391 2034 2130
Size: 391 1643 96
Fragments arranged by size:
1643 391 96
BbvCI CC'TCA_GC
Cuts at: 0 205 548 1159 2130
Size: 205 343 611 971
Fragments arranged by size:
971 611 343 205
BglI GCCn_nnn'nGGC
Cuts at: 0 137 289 329 883 2130
Size: 137 152 40 554 1247
Fragments arranged by size:
1247 554 152 137 40
BglII A'GATC_T
Cuts at: 0 399 2130
Size: 399 1731
BlpI GC'TnA_GC
Cuts at: 0 596 2130
Size: 596 1534
Bme1580I G_kGCm'C
Cuts at: 0 133 1727 1735 1961 2130
Size: 133 1594 8 226 169
Fragments arranged by size:
1594 226 169 133 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 57 882 2130
Size: 57 825 1248
Fragments arranged by size:
1248 825 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 804 1617 2130
Size: 804 813 513
Fragments arranged by size:
813 804 513
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 991 1629 1703 2130
Size: 241 750 638 74 427
Fragments arranged by size:
750 638 427 241 74
Bpu10I CC'TnA_GC
Cuts at: 0 205 548 1159 1420 1753 2130
Size: 205 343 611 261 333 377
Fragments arranged by size:
611 377 343 333 261 205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 1057 1656 2130
Size: 698 359 599 474
Fragments arranged by size:
698 599 474 359
BsaI GGTCTCn'nnnn_
Cuts at: 0 52 245 473 2130
Size: 52 193 228 1657
Fragments arranged by size:
1657 228 193 52
BsaBI GATnn'nnATC
Cuts at: 0 985 2130
Size: 985 1145
BsaHI Gr'CG_yC
Cuts at: 0 857 2130
Size: 857 1273
BsaWI w'CCGG_w
Cuts at: 0 299 2130
Size: 299 1831
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 70 100 227 257 624 654 1006 1036
Size: 70 30 127 30 367 30 352 30
Cuts at: 1036 1477 1507 2130
Size: 441 30 623
Fragments arranged by size:
623 441 367 352 127 70 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 604 628 1011 1201 1368 1489 2130
Size: 604 24 383 190 167 121 641
Fragments arranged by size:
641 604 383 190 167 121 24
BseYI C'CCAG_C
Cuts at: 0 151 268 327 441 527 722 915 992
Size: 151 117 59 114 86 195 193 77
Cuts at: 992 1082 1528 1735 1768 1921 1961 2130
Size: 90 446 207 33 153 40 169
Fragments arranged by size:
446 207 195 193 169 153 151 117
114 90 86 77 59 40 33
BsiEI CG_ry'CG
Cuts at: 0 844 2130
Size: 844 1286
BsiHKAI G_wGCw'C
Cuts at: 0 785 929 936 1834 2018 2130
Size: 785 144 7 898 184 112
Fragments arranged by size:
898 785 184 144 112 7
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1576 2130
Size: 1576 554
Bsp1286I G_dGCh'C
Cuts at: 0 121 133 448 707 785 820 929 936
Size: 121 12 315 259 78 35 109 7
Cuts at: 936 1426 1727 1735 1834 1961 2018 2130
Size: 490 301 8 99 127 57 112
Fragments arranged by size:
490 315 301 259 127 121 112 109
99 78 57 35 12 8 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1772 2130
Size: 1772 358
BsrBI CCG'CTC
Cuts at: 0 225 2130
Size: 225 1905
BsrDI GCAATG_nn'
Cuts at: 0 158 2130
Size: 158 1972
BsrFI r'CCGG_y
Cuts at: 0 839 904 1067 1487 2130
Size: 839 65 163 420 643
Fragments arranged by size:
839 643 420 163 65
BsrGI T'GTAC_A
Cuts at: 0 77 2130
Size: 77 2053
BssHII G'CGCG_C
Cuts at: 0 214 873 1029 2130
Size: 214 659 156 1101
Fragments arranged by size:
1101 659 214 156
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1956 2130
Size: 1956 174
BstEII G'GTnAC_C
Cuts at: 0 1661 2130
Size: 1661 469
BstYI r'GATC_y
Cuts at: 0 399 980 1201 1332 1370 2130
Size: 399 581 221 131 38 760
Fragments arranged by size:
760 581 399 221 131 38
Bsu36I CC'TnA_GG
Cuts at: 0 421 467 623 2130
Size: 421 46 156 1507
Fragments arranged by size:
1507 421 156 46
BtgI C'CryG_G
Cuts at: 0 881 1872 2130
Size: 881 991 258
Fragments arranged by size:
991 881 258
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 972 2130
Size: 972 1158
BtsI GCAGTG_nn'
Cuts at: 0 314 513 2130
Size: 314 199 1617
Fragments arranged by size:
1617 314 199
DraIII CAC_nnn'GTG
Cuts at: 0 1612 1956 2130
Size: 1612 344 174
Fragments arranged by size:
1612 344 174
DrdI GACnn_nn'nnGTC
Cuts at: 0 487 2130
Size: 487 1643
EaeI y'GGCC_r
Cuts at: 0 290 752 841 944 2130
Size: 290 462 89 103 1186
Fragments arranged by size:
1186 462 290 103 89
EagI C'GGCC_G
Cuts at: 0 841 2130
Size: 841 1289
EarI CTCTTCn'nnn_
Cuts at: 0 50 605 678 689 790 1205 1345 2130
Size: 50 555 73 11 101 415 140 785
Fragments arranged by size:
785 555 415 140 101 73 50 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 139 1032 1566 2103 2130
Size: 139 893 534 537 27
Fragments arranged by size:
893 537 534 139 27
EcoICRI GAG'CTC
Cuts at: 0 783 1832 2130
Size: 783 1049 298
Fragments arranged by size:
1049 783 298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 659 720 991 1629 1703 2130
Size: 241 418 61 271 638 74 427
Fragments arranged by size:
638 427 418 271 241 74 61
EcoO109I rG'GnC_Cy
Cuts at: 0 129 130 264 637 718 1255 1626 1723
Size: 129 1 134 373 81 537 371 97
Cuts at: 1723 1724 1731 1732 2099 2130
Size: 1 7 1 367 31
Fragments arranged by size:
537 373 371 367 134 129 97 81
31 7 1 1 1
HaeII r_GCGC'y
Cuts at: 0 860 999 1404 2130
Size: 860 139 405 726
Fragments arranged by size:
860 726 405 139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 624 655 656 687 1389 1421 1538 1570
Size: 624 31 1 31 702 32 117 32
Cuts at: 1570 1618 1650 2130
Size: 48 32 480
Fragments arranged by size:
702 624 480 117 48 32 32 32
31 31 1
HindIII A'AGCT_T
Cuts at: 0 568 1582 2130
Size: 568 1014 548
Fragments arranged by size:
1014 568 548
KasI G'GCGC_C
Cuts at: 0 856 2130
Size: 856 1274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1304 1569 2130
Size: 1304 265 561
Fragments arranged by size:
1304 561 265
MscI TGG'CCA
Cuts at: 0 292 2130
Size: 292 1838
MspA1I CmG'CkG
Cuts at: 0 39 391 762 966 1082 1392 1516 2130
Size: 39 352 371 204 116 310 124 614
Fragments arranged by size:
614 371 352 310 204 124 116 39
NarI GG'CG_CC
Cuts at: 0 857 2130
Size: 857 1273
NspI r_CATG'y
Cuts at: 0 1456 2130
Size: 1456 674
PasI CC'CwG_GG
Cuts at: 0 538 1657 1728 1967 2130
Size: 538 1119 71 239 163
Fragments arranged by size:
1119 538 239 163 71
PflMI CCAn_nnn'nTGG
Cuts at: 0 729 2130
Size: 729 1401
PfoI T'CCnGG_A
Cuts at: 0 728 1181 2130
Size: 728 453 949
Fragments arranged by size:
949 728 453
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1006 1038 2130
Size: 1006 32 1092
Fragments arranged by size:
1092 1006 32
PpuMI rG'GwC_Cy
Cuts at: 0 264 637 718 1626 2099 2130
Size: 264 373 81 908 473 31
Fragments arranged by size:
908 473 373 264 81 31
PspOMI G'GGCC_C
Cuts at: 0 129 1723 1731 2130
Size: 129 1594 8 399
Fragments arranged by size:
1594 399 129 8
PstI C_TGCA'G
Cuts at: 0 44 458 855 1397 2130
Size: 44 414 397 542 733
Fragments arranged by size:
733 542 414 397 44
PvuII CAG'CTG
Cuts at: 0 762 1082 1392 1516 2130
Size: 762 320 310 124 614
Fragments arranged by size:
762 614 320 310 124
SacI G_AGCT'C
Cuts at: 0 785 1834 2130
Size: 785 1049 296
Fragments arranged by size:
1049 785 296
SanDI GG'GwC_CC
Cuts at: 0 1626 2099 2130
Size: 1626 473 31
Fragments arranged by size:
1626 473 31
SapI GCTCTTCn'nnn_
Cuts at: 0 790 2130
Size: 790 1340
SexAI A'CCwGG_T
Cuts at: 0 1289 2130
Size: 1289 841
SfcI C'TryA_G
Cuts at: 0 40 454 851 1393 2130
Size: 40 414 397 542 737
Fragments arranged by size:
737 542 414 397 40
SfoI GGC'GCC
Cuts at: 0 858 2130
Size: 858 1272
SmaI CCC'GGG
Cuts at: 0 135 369 1498 1623 2130
Size: 135 234 1129 125 507
Fragments arranged by size:
1129 507 234 135 125
SmlI C'TyrA_G
Cuts at: 0 713 1072 1671 2130
Size: 713 359 599 459
Fragments arranged by size:
713 599 459 359
SphI G_CATG'C
Cuts at: 0 1456 2130
Size: 1456 674
StuI AGG'CCT
Cuts at: 0 682 2130
Size: 682 1448
StyI C'CwwG_G
Cuts at: 0 791 1269 2130
Size: 791 478 861
Fragments arranged by size:
861 791 478
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 76 2130
Size: 76 2054
TatI w'GTAC_w
Cuts at: 0 77 2130
Size: 77 2053
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 227 259 2130
Size: 227 32 1871
Fragments arranged by size:
1871 227 32
XmaI C'CCGG_G
Cuts at: 0 133 367 1496 1621 2130
Size: 133 234 1129 125 509
Fragments arranged by size:
1129 509 234 133 125
Enzymes that do cut:
AcuI AfeI AlfI AloI AlwNI ApaI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
HaeII Hin4I HindIII KasI MmeI MscI MspA1I NarI
NspI PasI PflMI PfoI PpiI PpuMI PspOMI PstI
PvuII SacI SanDI SapI SexAI SfcI SfoI SmaI
SmlI SphI StuI StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI ApaLI ApoI AscI AseI AsiSI AvrII
BcgI BciVI BclI BfrBI BmgBI BmtI BsaAI BsgI
BsiWI BsmI BspEI BspHI BssSI BstBI BstXI BstZ17I
ClaI CspCI DraI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HpaI KpnI MfeI MluI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SbfI ScaI SfiI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
XcmI XhoI XmnI ZraI