Restriction map for
pFN21AE4196
(Linear) (Six-base) MAPSORT of: KIEE4196.seq Check: 6408 from: 1 to: 2118
KIEE4196.orf
With 182 enzymes: SgfI *
March 22, 2012 17:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 851 2118
Size: 851 1267
AccI GT'mk_AC
Cuts at: 0 703 2118
Size: 703 1415
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 2118
Size: 745 1373
AflIII A'CryG_T
Cuts at: 0 1085 1973 2118
Size: 1085 888 145
Fragments arranged by size:
1085 888 145
AleI CACnn'nnGTG
Cuts at: 0 1072 2118
Size: 1072 1046
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 559 1090 1171 2118
Size: 559 531 81 947
Fragments arranged by size:
947 559 531 81
AlwNI CAG_nnn'CTG
Cuts at: 0 166 201 2118
Size: 166 35 1917
Fragments arranged by size:
1917 166 35
ApaI G_GGCC'C
Cuts at: 0 1119 1569 2118
Size: 1119 450 549
Fragments arranged by size:
1119 549 450
ApoI r'AATT_y
Cuts at: 0 988 2118
Size: 988 1130
AvaI C'yCGr_G
Cuts at: 0 1954 1963 2118
Size: 1954 9 155
Fragments arranged by size:
1954 155 9
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1215 1248 2118
Size: 1215 33 870
Fragments arranged by size:
1215 870 33
BamHI G'GATC_C
Cuts at: 0 1769 2118
Size: 1769 349
BanI G'GyrC_C
Cuts at: 0 116 905 1950 2039 2118
Size: 116 789 1045 89 79
Fragments arranged by size:
1045 789 116 89 79
BanII G_rGCy'C
Cuts at: 0 176 1043 1119 1379 1437 1569 2069 2118
Size: 176 867 76 260 58 132 500 49
Fragments arranged by size:
867 500 260 176 132 76 58 49
BbeI G_GCGC'C
Cuts at: 0 120 2118
Size: 120 1998
BbsI GAAGACnn'nnnn_
Cuts at: 0 733 2118
Size: 733 1385
BbvCI CC'TCA_GC
Cuts at: 0 1304 2118
Size: 1304 814
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2086 2118
Size: 2086 32
BciVI GTATCCnnnnn_n'
Cuts at: 0 358 624 2118
Size: 358 266 1494
Fragments arranged by size:
1494 358 266
BglI GCCn_nnn'nGGC
Cuts at: 0 125 2118
Size: 125 1993
Bme1580I G_kGCm'C
Cuts at: 0 1119 1569 2044 2118
Size: 1119 450 475 74
Fragments arranged by size:
1119 475 450 74
BmrI ACTGGGnnnn_n'
Cuts at: 0 916 1440 2118
Size: 916 524 678
Fragments arranged by size:
916 678 524
BmtI G_CTAG'C
Cuts at: 0 1540 2118
Size: 1540 578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 672 1129 1402 1528 1674 2118
Size: 672 457 273 126 146 444
Fragments arranged by size:
672 457 444 273 146 126
Bpu10I CC'TnA_GC
Cuts at: 0 1304 2118
Size: 1304 814
BsaI GGTCTCn'nnnn_
Cuts at: 0 193 2118
Size: 193 1925
BsaAI yAC'GTr
Cuts at: 0 1086 1134 1974 2118
Size: 1086 48 840 144
Fragments arranged by size:
1086 840 144 48
BsaHI Gr'CG_yC
Cuts at: 0 117 1960 2118
Size: 117 1843 158
Fragments arranged by size:
1843 158 117
BsaWI w'CCGG_w
Cuts at: 0 1721 2118
Size: 1721 397
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 889 919 2118
Size: 889 30 1199
Fragments arranged by size:
1199 889 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 823 1321 1569 2118
Size: 823 498 248 549
Fragments arranged by size:
823 549 498 248
BseYI C'CCAG_C
Cuts at: 0 153 166 278 333 438 681 1027 1035
Size: 153 13 112 55 105 243 346 8
Cuts at: 1035 1052 1269 1622 2018 2118
Size: 17 217 353 396 100
Fragments arranged by size:
396 353 346 243 217 153 112 105
100 55 17 13 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 429 1114 1131 1876 2034 2118
Size: 85 344 685 17 745 158 84
Fragments arranged by size:
745 685 344 158 85 84 17
BsiHKAI G_wGCw'C
Cuts at: 0 1069 1379 2069 2118
Size: 1069 310 690 49
Fragments arranged by size:
1069 690 310 49
BsmBI CGTCTCn'nnnn_
Cuts at: 0 604 1951 2118
Size: 604 1347 167
Fragments arranged by size:
1347 604 167
Bsp1286I G_dGCh'C
Cuts at: 0 176 1043 1069 1119 1379 1437 1569 2044
Size: 176 867 26 50 260 58 132 475
Cuts at: 2044 2069 2118
Size: 25 49
Fragments arranged by size:
867 475 260 176 132 58 50 49
26 25
BspEI T'CCGG_A
Cuts at: 0 1721 2118
Size: 1721 397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 533 851 2118
Size: 533 318 1267
Fragments arranged by size:
1267 533 318
BsrBI CCG'CTC
Cuts at: 0 1908 2118
Size: 1908 210
BsrDI GCAATG_nn'
Cuts at: 0 881 1368 2118
Size: 881 487 750
Fragments arranged by size:
881 750 487
BsrFI r'CCGG_y
Cuts at: 0 1928 2118
Size: 1928 190
BsrGI T'GTAC_A
Cuts at: 0 248 2118
Size: 248 1870
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1137 2118
Size: 1137 981
BstBI TT'CG_AA
Cuts at: 0 1551 2118
Size: 1551 567
BstXI CCAn_nnnn'nTGG
Cuts at: 0 333 387 894 2118
Size: 333 54 507 1224
Fragments arranged by size:
1224 507 333 54
BstYI r'GATC_y
Cuts at: 0 1769 2118
Size: 1769 349
Bsu36I CC'TnA_GG
Cuts at: 0 626 1840 2118
Size: 626 1214 278
Fragments arranged by size:
1214 626 278
BtgI C'CryG_G
Cuts at: 0 59 272 449 635 1896 2118
Size: 59 213 177 186 1261 222
Fragments arranged by size:
1261 222 213 186 177 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 419 1716 2118
Size: 419 1297 402
Fragments arranged by size:
1297 419 402
BtsI GCAGTG_nn'
Cuts at: 0 140 1387 2102 2118
Size: 140 1247 715 16
Fragments arranged by size:
1247 715 140 16
ClaI AT'CG_AT
Cuts at: 0 1250 2118
Size: 1250 868
DrdI GACnn_nn'nnGTC
Cuts at: 0 1965 2118
Size: 1965 153
EaeI y'GGCC_r
Cuts at: 0 179 2118
Size: 179 1939
EarI CTCTTCn'nnn_
Cuts at: 0 152 1010 2057 2076 2118
Size: 152 858 1047 19 42
Fragments arranged by size:
1047 858 152 42 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 561 1450 1897 2067 2118
Size: 561 889 447 170 51
Fragments arranged by size:
889 561 447 170 51
EcoICRI GAG'CTC
Cuts at: 0 1377 2067 2118
Size: 1377 690 51
Fragments arranged by size:
1377 690 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 672 745 1129 1402 1528 1674 2118
Size: 672 73 384 273 126 146 444
Fragments arranged by size:
672 444 384 273 146 126 73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1699 2118
Size: 1699 419
EcoO109I rG'GnC_Cy
Cuts at: 0 284 522 585 825 1116 1565 1566 1646
Size: 284 238 63 240 291 449 1 80
Cuts at: 1646 2118
Size: 472
Fragments arranged by size:
472 449 291 284 240 238 80 63
1
EcoRV GAT'ATC
Cuts at: 0 1887 2118
Size: 1887 231
HaeII r_GCGC'y
Cuts at: 0 120 167 1935 2118
Size: 120 47 1768 183
Fragments arranged by size:
1768 183 120 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 276 308 401 433 2118
Size: 276 32 93 32 1685
Fragments arranged by size:
1685 276 93 32 32
HincII GTy'rAC
Cuts at: 0 704 2118
Size: 704 1414
KasI G'GCGC_C
Cuts at: 0 116 2118
Size: 116 2002
MscI TGG'CCA
Cuts at: 0 181 2118
Size: 181 1937
MslI CAynn'nnrTG
Cuts at: 0 20 385 922 1072 2118
Size: 20 365 537 150 1046
Fragments arranged by size:
1046 537 365 150 20
MspA1I CmG'CkG
Cuts at: 0 302 773 1878 1898 1919 2037 2118
Size: 302 471 1105 20 21 118 81
Fragments arranged by size:
1105 471 302 118 81 21 20
NaeI GCC'GGC
Cuts at: 0 1930 2118
Size: 1930 188
NarI GG'CG_CC
Cuts at: 0 117 2118
Size: 117 2001
NcoI C'CATG_G
Cuts at: 0 272 635 2118
Size: 272 363 1483
Fragments arranged by size:
1483 363 272
NgoMIV G'CCGG_C
Cuts at: 0 1928 2118
Size: 1928 190
NheI G'CTAG_C
Cuts at: 0 1536 2118
Size: 1536 582
NspI r_CATG'y
Cuts at: 0 1171 1611 2031 2118
Size: 1171 440 420 87
Fragments arranged by size:
1171 440 420 87
PasI CC'CwG_GG
Cuts at: 0 657 1119 2118
Size: 657 462 999
Fragments arranged by size:
999 657 462
PflMI CCAn_nnn'nTGG
Cuts at: 0 278 1629 1878 2118
Size: 278 1351 249 240
Fragments arranged by size:
1351 278 249 240
PfoI T'CCnGG_A
Cuts at: 0 1379 2118
Size: 1379 739
PmlI CAC'GTG
Cuts at: 0 1134 2118
Size: 1134 984
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1564 1596 2118
Size: 1564 32 522
Fragments arranged by size:
1564 522 32
PpuMI rG'GwC_Cy
Cuts at: 0 284 522 585 825 2118
Size: 284 238 63 240 1293
Fragments arranged by size:
1293 284 240 238 63
PspOMI G'GGCC_C
Cuts at: 0 1115 1565 2118
Size: 1115 450 553
Fragments arranged by size:
1115 553 450
PstI C_TGCA'G
Cuts at: 0 461 1481 1665 2118
Size: 461 1020 184 453
Fragments arranged by size:
1020 461 453 184
PvuII CAG'CTG
Cuts at: 0 773 2118
Size: 773 1345
SacI G_AGCT'C
Cuts at: 0 1379 2069 2118
Size: 1379 690 49
Fragments arranged by size:
1379 690 49
SacII CC_GC'GG
Cuts at: 0 1899 2118
Size: 1899 219
SalI G'TCGA_C
Cuts at: 0 702 2118
Size: 702 1416
SanDI GG'GwC_CC
Cuts at: 0 825 2118
Size: 825 1293
SapI GCTCTTCn'nnn_
Cuts at: 0 152 2057 2118
Size: 152 1905 61
Fragments arranged by size:
1905 152 61
ScaI AGT'ACT
Cuts at: 0 1483 2118
Size: 1483 635
SfcI C'TryA_G
Cuts at: 0 99 457 1477 1661 2118
Size: 99 358 1020 184 457
Fragments arranged by size:
1020 457 358 184 99
SfoI GGC'GCC
Cuts at: 0 118 2118
Size: 118 2000
SpeI A'CTAG_T
Cuts at: 0 318 2118
Size: 318 1800
SphI G_CATG'C
Cuts at: 0 1171 2118
Size: 1171 947
SspI AAT'ATT
Cuts at: 0 1002 2118
Size: 1002 1116
StuI AGG'CCT
Cuts at: 0 1348 2118
Size: 1348 770
StyI C'CwwG_G
Cuts at: 0 272 371 635 887 2118
Size: 272 99 264 252 1231
Fragments arranged by size:
1231 272 264 252 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 853 2118
Size: 853 1265
TatI w'GTAC_w
Cuts at: 0 248 1481 2003 2118
Size: 248 1233 522 115
Fragments arranged by size:
1233 522 248 115
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 889 921 2118
Size: 889 32 1197
Fragments arranged by size:
1197 889 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 970 2118
Size: 970 1148
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApaI
ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BglI Bme1580I BmrI BmtI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HincII KasI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI
PstI PvuII SacI SacII SalI SanDI SapI ScaI
SfcI SfoI SpeI SphI SspI StuI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaLI AscI AseI AsiSI AvrII BclI BfrBI BglII
BlpI BmgBI BplI BpuEI BsaBI BsiEI BsiWI BsmI
BspHI BssHII BssSI BstEII BstZ17I CspCI DraI DraIII
EagI EcoRI FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MmeI NdeI NotI NruI NsiI
PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI
RsrII SbfI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SrfI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI