Restriction map for
pFN21AE4195
(Linear) (Six-base) MAPSORT of: KIEE4195.seq Check: 1791 from: 1 to: 2112
KIEE4195.orf
With 182 enzymes: SgfI *
September 21, 2016 11:20 ..
AccI GT'mk_AC
Cuts at: 0 154 2112
Size: 154 1958
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 1046 1151 1508 2014 2042 2112
Size: 1022 24 105 357 506 28 70
Fragments arranged by size:
1022 506 357 105 70 28 24
AflII C'TTAA_G
Cuts at: 0 57 2112
Size: 57 2055
AflIII A'CryG_T
Cuts at: 0 1996 1998 2112
Size: 1996 2 114
Fragments arranged by size:
1996 114 2
AleI CACnn'nnGTG
Cuts at: 0 488 2112
Size: 488 1624
AlwNI CAG_nnn'CTG
Cuts at: 0 172 1000 2112
Size: 172 828 1112
Fragments arranged by size:
1112 828 172
ApaLI G'TGCA_C
Cuts at: 0 1713 2112
Size: 1713 399
ApoI r'AATT_y
Cuts at: 0 32 657 672 927 936 1113 1533 1658
Size: 32 625 15 255 9 177 420 125
Cuts at: 1658 2112
Size: 454
Fragments arranged by size:
625 454 420 255 177 125 32 15
9
BanII G_rGCy'C
Cuts at: 0 1878 2112
Size: 1878 234
BbsI GAAGACnn'nnnn_
Cuts at: 0 2084 2112
Size: 2084 28
BbvCI CC'TCA_GC
Cuts at: 0 303 2112
Size: 303 1809
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 825 859 2112
Size: 825 34 1253
Fragments arranged by size:
1253 825 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1226 2112
Size: 1226 886
BclI T'GATC_A
Cuts at: 0 1502 2112
Size: 1502 610
BglII A'GATC_T
Cuts at: 0 376 892 1258 2046 2112
Size: 376 516 366 788 66
Fragments arranged by size:
788 516 376 366 66
Bme1580I G_kGCm'C
Cuts at: 0 1717 2112
Size: 1717 395
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1864 2112
Size: 1864 248
Bpu10I CC'TnA_GC
Cuts at: 0 303 2112
Size: 303 1809
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 216 1385 2112
Size: 99 117 1169 727
Fragments arranged by size:
1169 727 117 99
BsaI GGTCTCn'nnnn_
Cuts at: 0 1924 2078 2112
Size: 1924 154 34
Fragments arranged by size:
1924 154 34
BsaAI yAC'GTr
Cuts at: 0 1999 2112
Size: 1999 113
BsaBI GATnn'nnATC
Cuts at: 0 107 381 2112
Size: 107 274 1731
Fragments arranged by size:
1731 274 107
BsaWI w'CCGG_w
Cuts at: 0 1451 2112
Size: 1451 661
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 348 378 2112
Size: 348 30 1734
Fragments arranged by size:
1734 348 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 441 852 2112
Size: 441 411 1260
Fragments arranged by size:
1260 441 411
BseYI C'CCAG_C
Cuts at: 0 307 1467 2112
Size: 307 1160 645
Fragments arranged by size:
1160 645 307
BsiHKAI G_wGCw'C
Cuts at: 0 228 577 1717 1878 2112
Size: 228 349 1140 161 234
Fragments arranged by size:
1140 349 234 228 161
Bsp1286I G_dGCh'C
Cuts at: 0 228 577 1717 1878 2112
Size: 228 349 1140 161 234
Fragments arranged by size:
1140 349 234 228 161
BspHI T'CATG_A
Cuts at: 0 258 1499 1860 2112
Size: 258 1241 361 252
Fragments arranged by size:
1241 361 258 252
BsrGI T'GTAC_A
Cuts at: 0 286 2112
Size: 286 1826
BstYI r'GATC_y
Cuts at: 0 376 892 1258 1581 2046 2112
Size: 376 516 366 323 465 66
Fragments arranged by size:
516 465 376 366 323 66
BtgI C'CryG_G
Cuts at: 0 1150 2112
Size: 1150 962
BtsI GCAGTG_nn'
Cuts at: 0 1371 2112
Size: 1371 741
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1271 1306 2112
Size: 1271 35 806
Fragments arranged by size:
1271 806 35
DraI TTT'AAA
Cuts at: 0 98 2112
Size: 98 2014
EarI CTCTTCn'nnn_
Cuts at: 0 129 1209 1269 2033 2112
Size: 129 1080 60 764 79
Fragments arranged by size:
1080 764 129 79 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 843 2112
Size: 843 1269
EcoICRI GAG'CTC
Cuts at: 0 1876 2112
Size: 1876 236
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 1046 1151 1508 1864 2014 2042 2112
Size: 1022 24 105 357 356 150 28 70
Fragments arranged by size:
1022 357 356 150 105 70 28 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1621 2112
Size: 1621 491
EcoRI G'AATT_C
Cuts at: 0 927 1533 2112
Size: 927 606 579
Fragments arranged by size:
927 606 579
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 140 1589 2112
Size: 140 1449 523
Fragments arranged by size:
1449 523 140
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 348 380 1237 1269 2112
Size: 348 32 857 32 843
Fragments arranged by size:
857 843 348 32 32
HindIII A'AGCT_T
Cuts at: 0 1028 1956 2112
Size: 1028 928 156
Fragments arranged by size:
1028 928 156
MfeI C'AATT_G
Cuts at: 0 610 2112
Size: 610 1502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 1510 1811 2112
Size: 300 1210 301 301
Fragments arranged by size:
1210 301 301 300
MslI CAynn'nnrTG
Cuts at: 0 488 1710 1883 2112
Size: 488 1222 173 229
Fragments arranged by size:
1222 488 229 173
MspA1I CmG'CkG
Cuts at: 0 307 1540 1675 2112
Size: 307 1233 135 437
Fragments arranged by size:
1233 437 307 135
NcoI C'CATG_G
Cuts at: 0 1150 2112
Size: 1150 962
NdeI CA'TA_TG
Cuts at: 0 513 2039 2112
Size: 513 1526 73
Fragments arranged by size:
1526 513 73
NspI r_CATG'y
Cuts at: 0 870 2112
Size: 870 1242
PflMI CCAn_nnn'nTGG
Cuts at: 0 1474 2112
Size: 1474 638
PmlI CAC'GTG
Cuts at: 0 1999 2112
Size: 1999 113
PstI C_TGCA'G
Cuts at: 0 1356 2112
Size: 1356 756
PvuII CAG'CTG
Cuts at: 0 307 1540 1675 2112
Size: 307 1233 135 437
Fragments arranged by size:
1233 437 307 135
SacI G_AGCT'C
Cuts at: 0 1878 2112
Size: 1878 234
SfcI C'TryA_G
Cuts at: 0 1352 2112
Size: 1352 760
SmlI C'TyrA_G
Cuts at: 0 57 114 231 1400 2112
Size: 57 57 117 1169 712
Fragments arranged by size:
1169 712 117 57 57
StyI C'CwwG_G
Cuts at: 0 1150 2112
Size: 1150 962
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1504 2112
Size: 1504 608
TatI w'GTAC_w
Cuts at: 0 286 295 2112
Size: 286 9 1817
Fragments arranged by size:
1817 286 9
Tth111I GACn'n_nGTC
Cuts at: 0 1495 2112
Size: 1495 617
XbaI T'CTAG_A
Cuts at: 0 1195 1255 2112
Size: 1195 60 857
Fragments arranged by size:
1195 857 60
XmnI GAAnn'nnTTC
Cuts at: 0 33 450 1292 2112
Size: 33 417 842 820
Fragments arranged by size:
842 820 417 33
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlwNI ApaLI ApoI
BanII BbsI BbvCI BcgI BciVI BclI BglII Bme1580I
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI
BseRI BseYI BsiHKAI Bsp1286I BspHI BsrGI BstYI BtgI
BtsI CspCI DraI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI FalI Hin4I HindIII MfeI MmeI MslI MspA1I
NcoI NdeI NspI PflMI PmlI PstI PvuII SacI
SfcI SmlI StyI TaqII TatI Tth111I XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI
AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BfrBI BglI BlpI BmgBI BmrI
BmtI BplI BsaHI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraIII
DrdI EaeI EagI EcoO109I EcoRV FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MluI MscI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
XcmI XhoI XmaI ZraI