Restriction map for
pFN21AE4194
(Linear) (Six-base) MAPSORT of: KIEE4194.seq Check: 1070 from: 1 to: 2109
KIEE4194.orf
With 182 enzymes: SgfI *
July 3, 2012 18:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 223 2109
Size: 223 1886
AccI GT'mk_AC
Cuts at: 0 1655 2109
Size: 1655 454
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 610 766 1333 1717 2109
Size: 470 140 156 567 384 392
Fragments arranged by size:
567 470 392 384 156 140
AflII C'TTAA_G
Cuts at: 0 403 2109
Size: 403 1706
AlwNI CAG_nnn'CTG
Cuts at: 0 10 918 2109
Size: 10 908 1191
Fragments arranged by size:
1191 908 10
ApaI G_GGCC'C
Cuts at: 0 2064 2109
Size: 2064 45
ApoI r'AATT_y
Cuts at: 0 672 2053 2109
Size: 672 1381 56
Fragments arranged by size:
1381 672 56
BanI G'GyrC_C
Cuts at: 0 413 2109
Size: 413 1696
BanII G_rGCy'C
Cuts at: 0 2064 2109
Size: 2064 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 1934 2109
Size: 1934 175
BbvCI CC'TCA_GC
Cuts at: 0 243 2109
Size: 243 1866
BclI T'GATC_A
Cuts at: 0 570 2109
Size: 570 1539
BglI GCCn_nnn'nGGC
Cuts at: 0 1048 2109
Size: 1048 1061
BglII A'GATC_T
Cuts at: 0 1055 2109
Size: 1055 1054
BlpI GC'TnA_GC
Cuts at: 0 1282 2109
Size: 1282 827
Bme1580I G_kGCm'C
Cuts at: 0 2064 2109
Size: 2064 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 35 1358 1424 1994 2109
Size: 35 1323 66 570 115
Fragments arranged by size:
1323 570 115 66 35
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 512 1183 1684 1696 1937 2109
Size: 420 92 671 501 12 241 172
Fragments arranged by size:
671 501 420 241 172 92 12
Bpu10I CC'TnA_GC
Cuts at: 0 243 2109
Size: 243 1866
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 973 1126 2109
Size: 973 153 983
Fragments arranged by size:
983 973 153
BsaI GGTCTCn'nnnn_
Cuts at: 0 94 317 404 2109
Size: 94 223 87 1705
Fragments arranged by size:
1705 223 94 87
BsaAI yAC'GTr
Cuts at: 0 1753 2109
Size: 1753 356
BsaBI GATnn'nnATC
Cuts at: 0 569 2109
Size: 569 1540
BsaWI w'CCGG_w
Cuts at: 0 48 2109
Size: 48 2061
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 539 569 1302 1332 2109
Size: 539 30 733 30 777
Fragments arranged by size:
777 733 539 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 367 1370 2109
Size: 367 1003 739
Fragments arranged by size:
1003 739 367
BseYI C'CCAG_C
Cuts at: 0 1481 1486 2109
Size: 1481 5 623
Fragments arranged by size:
1481 623 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 1454 1787 2109
Size: 104 1350 333 322
Fragments arranged by size:
1350 333 322 104
BsiHKAI G_wGCw'C
Cuts at: 0 206 2109
Size: 206 1903
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1186 2109
Size: 1186 923
Bsp1286I G_dGCh'C
Cuts at: 0 206 2064 2109
Size: 206 1858 45
Fragments arranged by size:
1858 206 45
BspEI T'CCGG_A
Cuts at: 0 48 2109
Size: 48 2061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 223 1395 2109
Size: 223 1172 714
Fragments arranged by size:
1172 714 223
BsrBI CCG'CTC
Cuts at: 0 1445 1824 2109
Size: 1445 379 285
Fragments arranged by size:
1445 379 285
BsrDI GCAATG_nn'
Cuts at: 0 605 2109
Size: 605 1504
BsrGI T'GTAC_A
Cuts at: 0 612 2109
Size: 612 1497
BstYI r'GATC_y
Cuts at: 0 915 1055 1437 1670 2109
Size: 915 140 382 233 439
Fragments arranged by size:
915 439 382 233 140
BtgI C'CryG_G
Cuts at: 0 1418 1518 1560 1760 1775 2109
Size: 1418 100 42 200 15 334
Fragments arranged by size:
1418 334 200 100 42 15
BtsI GCAGTG_nn'
Cuts at: 0 312 2109
Size: 312 1797
DraIII CAC_nnn'GTG
Cuts at: 0 258 2109
Size: 258 1851
EaeI y'GGCC_r
Cuts at: 0 29 335 2109
Size: 29 306 1774
Fragments arranged by size:
1774 306 29
EarI CTCTTCn'nnn_
Cuts at: 0 446 588 791 951 1022 2109
Size: 446 142 203 160 71 1087
Fragments arranged by size:
1087 446 203 160 142 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 470 512 610 766 1183 1333 1684
Size: 420 50 42 98 156 417 150 351
Cuts at: 1684 1696 1717 1937 2109
Size: 12 21 220 172
Fragments arranged by size:
420 417 351 220 172 156 150 98
50 42 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1601 2109
Size: 1601 508
EcoO109I rG'GnC_Cy
Cuts at: 0 726 2109
Size: 726 1383
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 369 401 1045 1077 1429 1461 2109
Size: 369 32 644 32 352 32 648
Fragments arranged by size:
648 644 369 352 32 32 32
HincII GTy'rAC
Cuts at: 0 1148 2109
Size: 1148 961
HindIII A'AGCT_T
Cuts at: 0 501 592 937 2109
Size: 501 91 345 1172
Fragments arranged by size:
1172 501 345 91
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 537 2109
Size: 537 1572
MscI TGG'CCA
Cuts at: 0 31 337 2109
Size: 31 306 1772
Fragments arranged by size:
1772 306 31
MslI CAynn'nnrTG
Cuts at: 0 1423 2109
Size: 1423 686
MspA1I CmG'CkG
Cuts at: 0 634 2109
Size: 634 1475
NcoI C'CATG_G
Cuts at: 0 1418 1518 1760 1775 2109
Size: 1418 100 242 15 334
Fragments arranged by size:
1418 334 242 100 15
NspI r_CATG'y
Cuts at: 0 1575 1951 2109
Size: 1575 376 158
Fragments arranged by size:
1575 376 158
PflMI CCAn_nnn'nTGG
Cuts at: 0 535 2109
Size: 535 1574
PfoI T'CCnGG_A
Cuts at: 0 436 728 1673 1932 2109
Size: 436 292 945 259 177
Fragments arranged by size:
945 436 292 259 177
PpuMI rG'GwC_Cy
Cuts at: 0 726 2109
Size: 726 1383
PshAI GACnn'nnGTC
Cuts at: 0 86 2084 2109
Size: 86 1998 25
Fragments arranged by size:
1998 86 25
PspOMI G'GGCC_C
Cuts at: 0 2060 2109
Size: 2060 49
PvuII CAG'CTG
Cuts at: 0 634 2109
Size: 634 1475
SapI GCTCTTCn'nnn_
Cuts at: 0 588 2109
Size: 588 1521
ScaI AGT'ACT
Cuts at: 0 1839 2109
Size: 1839 270
SexAI A'CCwGG_T
Cuts at: 0 254 2109
Size: 254 1855
SfcI C'TryA_G
Cuts at: 0 518 2109
Size: 518 1591
SmlI C'TyrA_G
Cuts at: 0 403 988 1141 2109
Size: 403 585 153 968
Fragments arranged by size:
968 585 403 153
StuI AGG'CCT
Cuts at: 0 1769 2109
Size: 1769 340
StyI C'CwwG_G
Cuts at: 0 173 1418 1518 1760 1775 2109
Size: 173 1245 100 242 15 334
Fragments arranged by size:
1245 334 242 173 100 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 386 2109
Size: 386 1723
TatI w'GTAC_w
Cuts at: 0 612 1837 2109
Size: 612 1225 272
Fragments arranged by size:
1225 612 272
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 539 571 1302 1334 2109
Size: 539 32 731 32 775
Fragments arranged by size:
775 731 539 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1371 2109
Size: 1371 738
XmnI GAAnn'nnTTC
Cuts at: 0 1941 2109
Size: 1941 168
Enzymes that do cut:
AarI AccI AcuI AflII AlwNI ApaI ApoI BanI
BanII BbsI BbvCI BclI BglI BglII BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrGI BstYI BtgI BtsI DraIII
EaeI EarI Eco57MI EcoNI EcoO109I Hin4I HincII HindIII
MmeI MscI MslI MspA1I NcoI NspI PflMI PfoI
PpuMI PshAI PspOMI PvuII SapI ScaI SexAI SfcI
SmlI StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BcgI BciVI BfrBI BmgBI BmtI
BplI BsaHI BsiEI BsiWI BsmI BspHI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PsiI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmaI ZraI