Restriction map for
pFN21AE4192
(Linear) (Six-base) MAPSORT of: KIEE4192.seq Check: 4801 from: 1 to: 2079
KIEE4192.orf
With 182 enzymes: SgfI *
March 22, 2012 17:33 ..
AccI GT'mk_AC
Cuts at: 0 795 2079
Size: 795 1284
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 2079
Size: 215 1864
AfeI AGC'GCT
Cuts at: 0 614 2079
Size: 614 1465
AgeI A'CCGG_T
Cuts at: 0 946 2079
Size: 946 1133
AleI CACnn'nnGTG
Cuts at: 0 1928 2079
Size: 1928 151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 956 2079
Size: 956 1123
AlwNI CAG_nnn'CTG
Cuts at: 0 1741 2079
Size: 1741 338
ApoI r'AATT_y
Cuts at: 0 271 410 803 1238 1398 1537 1818 2079
Size: 271 139 393 435 160 139 281 261
Fragments arranged by size:
435 393 281 271 261 160 139 139
AseI AT'TA_AT
Cuts at: 0 1610 2079
Size: 1610 469
AvaI C'yCGr_G
Cuts at: 0 1307 2079
Size: 1307 772
BbsI GAAGACnn'nnnn_
Cuts at: 0 456 914 2079
Size: 456 458 1165
Fragments arranged by size:
1165 458 456
BciVI GTATCCnnnnn_n'
Cuts at: 0 31 2079
Size: 31 2048
BclI T'GATC_A
Cuts at: 0 183 2079
Size: 183 1896
BlpI GC'TnA_GC
Cuts at: 0 1262 2079
Size: 1262 817
BmgBI CAC'GTC
Cuts at: 0 748 2079
Size: 748 1331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1790 2079
Size: 1790 289
BsaWI w'CCGG_w
Cuts at: 0 946 2079
Size: 946 1133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1669 1910 1976 2079
Size: 1669 241 66 103
Fragments arranged by size:
1669 241 103 66
BsiHKAI G_wGCw'C
Cuts at: 0 1263 2079
Size: 1263 816
Bsp1286I G_dGCh'C
Cuts at: 0 1263 2079
Size: 1263 816
BsrDI GCAATG_nn'
Cuts at: 0 97 1877 2079
Size: 97 1780 202
Fragments arranged by size:
1780 202 97
BsrFI r'CCGG_y
Cuts at: 0 946 2079
Size: 946 1133
BssHII G'CGCG_C
Cuts at: 0 1668 2079
Size: 1668 411
BstAPI GCAn_nnn'nTGC
Cuts at: 0 385 1429 1888 2079
Size: 385 1044 459 191
Fragments arranged by size:
1044 459 385 191
BstBI TT'CG_AA
Cuts at: 0 447 2079
Size: 447 1632
BstYI r'GATC_y
Cuts at: 0 108 759 2079
Size: 108 651 1320
Fragments arranged by size:
1320 651 108
Bsu36I CC'TnA_GG
Cuts at: 0 1657 2079
Size: 1657 422
BtgI C'CryG_G
Cuts at: 0 104 2079
Size: 104 1975
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 824 2079
Size: 824 1255
BtsI GCAGTG_nn'
Cuts at: 0 820 1049 1110 1644 1898 2079
Size: 820 229 61 534 254 181
Fragments arranged by size:
820 534 254 229 181 61
DraIII CAC_nnn'GTG
Cuts at: 0 1591 2079
Size: 1591 488
EarI CTCTTCn'nnn_
Cuts at: 0 17 776 2079
Size: 17 759 1303
Fragments arranged by size:
1303 759 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 2079
Size: 215 1864
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1861 2079
Size: 1861 218
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 148 274 802 2079
Size: 148 126 528 1277
Fragments arranged by size:
1277 528 148 126
HaeII r_GCGC'y
Cuts at: 0 616 2079
Size: 616 1463
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 1498 1530 1780 1812 2079
Size: 26 1472 32 250 32 267
Fragments arranged by size:
1472 267 250 32 32 26
HindIII A'AGCT_T
Cuts at: 0 442 495 2079
Size: 442 53 1584
Fragments arranged by size:
1584 442 53
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 311 2034 2079
Size: 174 137 1723 45
Fragments arranged by size:
1723 174 137 45
MslI CAynn'nnrTG
Cuts at: 0 1928 2079
Size: 1928 151
MspA1I CmG'CkG
Cuts at: 0 841 2079
Size: 841 1238
NcoI C'CATG_G
Cuts at: 0 104 2079
Size: 104 1975
NdeI CA'TA_TG
Cuts at: 0 2000 2079
Size: 2000 79
NspI r_CATG'y
Cuts at: 0 472 1132 2079
Size: 472 660 947
Fragments arranged by size:
947 660 472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1301 1333 2079
Size: 1301 32 746
Fragments arranged by size:
1301 746 32
PsiI TTA'TAA
Cuts at: 0 32 2079
Size: 32 2047
PvuII CAG'CTG
Cuts at: 0 841 2079
Size: 841 1238
SapI GCTCTTCn'nnn_
Cuts at: 0 776 2079
Size: 776 1303
ScaI AGT'ACT
Cuts at: 0 738 2079
Size: 738 1341
SfcI C'TryA_G
Cuts at: 0 578 1553 1616 2079
Size: 578 975 63 463
Fragments arranged by size:
975 578 463 63
SmlI C'TyrA_G
Cuts at: 0 1307 1805 2079
Size: 1307 498 274
Fragments arranged by size:
1307 498 274
SspI AAT'ATT
Cuts at: 0 200 959 2022 2034 2079
Size: 200 759 1063 12 45
Fragments arranged by size:
1063 759 200 45 12
StyI C'CwwG_G
Cuts at: 0 104 2079
Size: 104 1975
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1246 2079
Size: 1246 833
TatI w'GTAC_w
Cuts at: 0 720 736 1185 1899 2079
Size: 720 16 449 714 180
Fragments arranged by size:
720 714 449 180 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 845 877 1469 1501 2079
Size: 845 32 592 32 578
Fragments arranged by size:
845 592 578 32 32
XhoI C'TCGA_G
Cuts at: 0 1307 2079
Size: 1307 772
Enzymes that do cut:
AccI AcuI AfeI AgeI AleI AlfI AlwNI ApoI
AseI AvaI BbsI BciVI BclI BlpI BmgBI BpuEI
BsaWI BsgI BsiHKAI Bsp1286I BsrDI BsrFI BssHII BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraIII EarI
Eco57MI EcoNI FalI HaeII Hin4I HindIII MmeI MslI
MspA1I NcoI NdeI NspI PpiI PsiI PvuII SapI
ScaI SfcI SmlI SspI StyI TaqII TatI TstI
XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AhdI AloI
ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BanI
BanII BbeI BbvCI BcgI BfrBI BglI BglII Bme1580I
BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrGI BssSI BstEII BstXI
BstZ17I ClaI CspCI DraI DrdI EaeI EagI EciI
EcoICRI EcoO109I EcoRI EcoRV FseI FspI FspAI HincII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI Tth111I XbaI XcmI
XmaI XmnI ZraI