Restriction map for
pFN21AE4191
(Linear) (Six-base) MAPSORT of: KIEE4191.seq Check: 2096 from: 1 to: 2064
KIEE4191.orf
With 182 enzymes: SgfI *
July 3, 2012 18:44 ..
Acc65I G'GTAC_C
Cuts at: 0 716 2064
Size: 716 1348
AflIII A'CryG_T
Cuts at: 0 191 443 614 728 2064
Size: 191 252 171 114 1336
Fragments arranged by size:
1336 252 191 171 114
AleI CACnn'nnGTG
Cuts at: 0 514 2064
Size: 514 1550
AlwNI CAG_nnn'CTG
Cuts at: 0 1198 1363 2064
Size: 1198 165 701
Fragments arranged by size:
1198 701 165
ApaI G_GGCC'C
Cuts at: 0 819 894 2064
Size: 819 75 1170
Fragments arranged by size:
1170 819 75
ApoI r'AATT_y
Cuts at: 0 844 2064
Size: 844 1220
AvaI C'yCGr_G
Cuts at: 0 649 2064
Size: 649 1415
BamHI G'GATC_C
Cuts at: 0 1738 2064
Size: 1738 326
BanI G'GyrC_C
Cuts at: 0 716 2064
Size: 716 1348
BanII G_rGCy'C
Cuts at: 0 66 692 819 832 894 1555 1995 2064
Size: 66 626 127 13 62 661 440 69
Fragments arranged by size:
661 626 440 127 69 66 62 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 407 2064
Size: 407 1657
BbvCI CC'TCA_GC
Cuts at: 0 83 316 1411 2045 2064
Size: 83 233 1095 634 19
Fragments arranged by size:
1095 634 233 83 19
BciVI GTATCCnnnnn_n'
Cuts at: 0 743 2064
Size: 743 1321
BglI GCCn_nnn'nGGC
Cuts at: 0 16 2064
Size: 16 2048
BlpI GC'TnA_GC
Cuts at: 0 550 2064
Size: 550 1514
Bme1580I G_kGCm'C
Cuts at: 0 819 894 2064
Size: 819 75 1170
Fragments arranged by size:
1170 819 75
BmgBI CAC'GTC
Cuts at: 0 192 444 2064
Size: 192 252 1620
Fragments arranged by size:
1620 252 192
BmrI ACTGGGnnnn_n'
Cuts at: 0 318 414 2064
Size: 318 96 1650
Fragments arranged by size:
1650 318 96
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 171 1583 1779 2064
Size: 171 1412 196 285
Fragments arranged by size:
1412 285 196 171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1513 1539 1900 2064
Size: 1513 26 361 164
Fragments arranged by size:
1513 361 164 26
Bpu10I CC'TnA_GC
Cuts at: 0 65 83 316 595 1411 2045 2064
Size: 65 18 233 279 816 634 19
Fragments arranged by size:
816 634 279 233 65 19 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 787 850 1225 1300 2064
Size: 787 63 375 75 764
Fragments arranged by size:
787 764 375 75 63
BsaAI yAC'GTr
Cuts at: 0 617 2064
Size: 617 1447
BsaHI Gr'CG_yC
Cuts at: 0 1497 2064
Size: 1497 567
BsaWI w'CCGG_w
Cuts at: 0 37 2064
Size: 37 2027
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1537 1567 2064
Size: 1537 30 497
Fragments arranged by size:
1537 497 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 103 106 667 1442 1644 2050 2064
Size: 100 3 3 561 775 202 406 14
Fragments arranged by size:
775 561 406 202 100 14 3 3
BseYI C'CCAG_C
Cuts at: 0 818 1345 1587 2064
Size: 818 527 242 477
Fragments arranged by size:
818 527 477 242
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 925 2064
Size: 925 1139
BsiEI CG_ry'CG
Cuts at: 0 80 1110 2064
Size: 80 1030 954
Fragments arranged by size:
1030 954 80
BsiHKAI G_wGCw'C
Cuts at: 0 66 1886 2064
Size: 66 1820 178
Fragments arranged by size:
1820 178 66
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1504 2064
Size: 1504 560
Bsp1286I G_dGCh'C
Cuts at: 0 66 692 819 832 894 1555 1886 1995
Size: 66 626 127 13 62 661 331 109
Cuts at: 1995 2064
Size: 69
Fragments arranged by size:
661 626 331 127 109 69 66 62
13
BspEI T'CCGG_A
Cuts at: 0 37 2064
Size: 37 2027
BspHI T'CATG_A
Cuts at: 0 1073 2064
Size: 1073 991
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 359 728 956 1190 2018 2064
Size: 359 369 228 234 828 46
Fragments arranged by size:
828 369 359 234 228 46
BsrBI CCG'CTC
Cuts at: 0 155 759 2064
Size: 155 604 1305
Fragments arranged by size:
1305 604 155
BsrDI GCAATG_nn'
Cuts at: 0 391 1911 2064
Size: 391 1520 153
Fragments arranged by size:
1520 391 153
BsrFI r'CCGG_y
Cuts at: 0 269 2064
Size: 269 1795
BssSI C'ACGA_G
Cuts at: 0 628 2064
Size: 628 1436
BstAPI GCAn_nnn'nTGC
Cuts at: 0 948 2064
Size: 948 1116
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1353 1831 1878 2064
Size: 1353 478 47 186
Fragments arranged by size:
1353 478 186 47
BstYI r'GATC_y
Cuts at: 0 168 668 1738 2064
Size: 168 500 1070 326
Fragments arranged by size:
1070 500 326 168
Bsu36I CC'TnA_GG
Cuts at: 0 833 914 2064
Size: 833 81 1150
Fragments arranged by size:
1150 833 81
BtgI C'CryG_G
Cuts at: 0 1115 2064
Size: 1115 949
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 614 2064
Size: 614 1450
BtsI GCAGTG_nn'
Cuts at: 0 332 1645 2064
Size: 332 1313 419
Fragments arranged by size:
1313 419 332
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1110 1145 2064
Size: 1110 35 919
Fragments arranged by size:
1110 919 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1570 2064
Size: 1570 494
EaeI y'GGCC_r
Cuts at: 0 77 1085 1107 1118 1374 1463 1833 1868
Size: 77 1008 22 11 256 89 370 35
Cuts at: 1868 1928 2064
Size: 60 136
Fragments arranged by size:
1008 370 256 136 89 77 60 35
22 11
EagI C'GGCC_G
Cuts at: 0 77 1107 2064
Size: 77 1030 957
Fragments arranged by size:
1030 957 77
EarI CTCTTCn'nnn_
Cuts at: 0 1213 2064
Size: 1213 851
EcoICRI GAG'CTC
Cuts at: 0 64 2064
Size: 64 2000
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1513 1539 1900 2064
Size: 1513 26 361 164
Fragments arranged by size:
1513 361 164 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 511 1186 1507 2064
Size: 511 675 321 557
Fragments arranged by size:
675 557 511 321
EcoO109I rG'GnC_Cy
Cuts at: 0 17 312 837 910 1480 1563 2064
Size: 17 295 525 73 570 83 501
Fragments arranged by size:
570 525 501 295 83 73 17
EcoRI G'AATT_C
Cuts at: 0 844 2064
Size: 844 1220
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 258 2064
Size: 258 1806
FspI TGC'GCA
Cuts at: 0 880 1903 2064
Size: 880 1023 161
Fragments arranged by size:
1023 880 161
HaeII r_GCGC'y
Cuts at: 0 46 940 1199 2064
Size: 46 894 259 865
Fragments arranged by size:
894 865 259 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 448 480 997 1029 1663 1695 2064
Size: 448 32 517 32 634 32 369
Fragments arranged by size:
634 517 448 369 32 32 32
HincII GTy'rAC
Cuts at: 0 1455 2064
Size: 1455 609
KpnI G_GTAC'C
Cuts at: 0 720 2064
Size: 720 1344
MfeI C'AATT_G
Cuts at: 0 355 2064
Size: 355 1709
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 564 1317 2064
Size: 464 100 753 747
Fragments arranged by size:
753 747 464 100
MscI TGG'CCA
Cuts at: 0 1120 1376 1465 1870 1930 2064
Size: 1120 256 89 405 60 134
Fragments arranged by size:
1120 405 256 134 89 60
MslI CAynn'nnrTG
Cuts at: 0 514 680 2064
Size: 514 166 1384
Fragments arranged by size:
1384 514 166
MspA1I CmG'CkG
Cuts at: 0 82 1223 1578 1591 2064
Size: 82 1141 355 13 473
Fragments arranged by size:
1141 473 355 82 13
NcoI C'CATG_G
Cuts at: 0 1115 2064
Size: 1115 949
NotI GC'GGCC_GC
Cuts at: 0 77 2064
Size: 77 1987
NspI r_CATG'y
Cuts at: 0 1278 1410 2064
Size: 1278 132 654
Fragments arranged by size:
1278 654 132
PfoI T'CCnGG_A
Cuts at: 0 1555 2064
Size: 1555 509
PpuMI rG'GwC_Cy
Cuts at: 0 312 837 1563 2064
Size: 312 525 726 501
Fragments arranged by size:
726 525 501 312
PsiI TTA'TAA
Cuts at: 0 1919 2064
Size: 1919 145
PspOMI G'GGCC_C
Cuts at: 0 815 890 2064
Size: 815 75 1174
Fragments arranged by size:
1174 815 75
PstI C_TGCA'G
Cuts at: 0 2015 2064
Size: 2015 49
PvuII CAG'CTG
Cuts at: 0 1578 1591 2064
Size: 1578 13 473
Fragments arranged by size:
1578 473 13
SacI G_AGCT'C
Cuts at: 0 66 2064
Size: 66 1998
SanDI GG'GwC_CC
Cuts at: 0 1563 2064
Size: 1563 501
SfcI C'TryA_G
Cuts at: 0 164 1733 2011 2064
Size: 164 1569 278 53
Fragments arranged by size:
1569 278 164 53
SmlI C'TyrA_G
Cuts at: 0 802 865 1240 1279 2064
Size: 802 63 375 39 785
Fragments arranged by size:
802 785 375 63 39
SphI G_CATG'C
Cuts at: 0 1278 2064
Size: 1278 786
StuI AGG'CCT
Cuts at: 0 1416 1711 2064
Size: 1416 295 353
Fragments arranged by size:
1416 353 295
StyI C'CwwG_G
Cuts at: 0 1115 2064
Size: 1115 949
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 989 1032 1601 1717 2064
Size: 989 43 569 116 347
Fragments arranged by size:
989 569 347 116 43
TatI w'GTAC_w
Cuts at: 0 873 2064
Size: 873 1191
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 488 520 1657 1689 2064
Size: 488 32 1137 32 375
Fragments arranged by size:
1137 488 375 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1725 2064
Size: 1725 339
XmnI GAAnn'nnTTC
Cuts at: 0 1846 2064
Size: 1846 218
Enzymes that do cut:
Acc65I AflIII AleI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BciVI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KpnI MfeI
MmeI MscI MslI MspA1I NcoI NotI NspI PfoI
PpuMI PsiI PspOMI PstI PvuII SacI SanDI SfcI
SmlI SphI StuI StyI TaqII TatI TstI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BclI BfrBI BglII BmtI BsaI
BsaBI BsiWI BsmI BsrGI BssHII BstBI BstEII BstZ17I
ClaI DraI DraIII EciI EcoRV FseI FspAI HindIII
HpaI KasI MluI NaeI NarI NdeI NgoMIV NheI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PspXI PsrI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
XhoI XmaI ZraI