Restriction map for
pFN21AE4188
(Linear) (Six-base) MAPSORT of: KIEE4188.seq Check: 5814 from: 1 to: 2022
KIEE4188.orf
With 182 enzymes: SgfI *
July 3, 2012 18:43 ..
AatII G_ACGT'C
Cuts at: 0 501 2022
Size: 501 1521
AccI GT'mk_AC
Cuts at: 0 377 1607 2022
Size: 377 1230 415
Fragments arranged by size:
1230 415 377
Acc65I G'GTAC_C
Cuts at: 0 1795 2022
Size: 1795 227
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 833 1534 1549 1913 2022
Size: 833 701 15 364 109
Fragments arranged by size:
833 701 364 109 15
AhdI GACnn_n'nnGTC
Cuts at: 0 357 1962 2022
Size: 357 1605 60
Fragments arranged by size:
1605 357 60
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1268 2022
Size: 1268 754
AlwNI CAG_nnn'CTG
Cuts at: 0 666 738 1011 1960 2022
Size: 666 72 273 949 62
Fragments arranged by size:
949 666 273 72 62
ApaI G_GGCC'C
Cuts at: 0 894 2022
Size: 894 1128
AseI AT'TA_AT
Cuts at: 0 1787 2022
Size: 1787 235
AvaI C'yCGr_G
Cuts at: 0 8 1086 2022
Size: 8 1078 936
Fragments arranged by size:
1078 936 8
BanI G'GyrC_C
Cuts at: 0 933 1795 2022
Size: 933 862 227
Fragments arranged by size:
933 862 227
BanII G_rGCy'C
Cuts at: 0 894 2022
Size: 894 1128
BbsI GAAGACnn'nnnn_
Cuts at: 0 20 774 1551 2022
Size: 20 754 777 471
Fragments arranged by size:
777 754 471 20
BglI GCCn_nnn'nGGC
Cuts at: 0 148 2022
Size: 148 1874
BglII A'GATC_T
Cuts at: 0 645 1571 2022
Size: 645 926 451
Fragments arranged by size:
926 645 451
BlpI GC'TnA_GC
Cuts at: 0 1196 2022
Size: 1196 826
Bme1580I G_kGCm'C
Cuts at: 0 894 2022
Size: 894 1128
BmgBI CAC'GTC
Cuts at: 0 696 2022
Size: 696 1326
BmrI ACTGGGnnnn_n'
Cuts at: 0 570 761 1021 1588 1609 2022
Size: 570 191 260 567 21 413
Fragments arranged by size:
570 567 413 260 191 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1471 1974 2022
Size: 1471 503 48
Fragments arranged by size:
1471 503 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 1361 2022
Size: 760 601 661
Fragments arranged by size:
760 661 601
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 514 2022
Size: 142 372 1508
Fragments arranged by size:
1508 372 142
BsaI GGTCTCn'nnnn_
Cuts at: 0 1322 1657 1662 1769 2022
Size: 1322 335 5 107 253
Fragments arranged by size:
1322 335 253 107 5
BsaBI GATnn'nnATC
Cuts at: 0 1036 2022
Size: 1036 986
BsaHI Gr'CG_yC
Cuts at: 0 17 211 353 498 2022
Size: 17 194 142 145 1524
Fragments arranged by size:
1524 194 145 142 17
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 415 424 445 454 2022
Size: 415 9 21 9 1568
Fragments arranged by size:
1568 415 21 9 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 646 1582 2022
Size: 646 936 440
Fragments arranged by size:
936 646 440
BseYI C'CCAG_C
Cuts at: 0 659 2022
Size: 659 1363
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 501 751 2022
Size: 501 250 1271
Fragments arranged by size:
1271 501 250
BsmI GAATG_Cn'
Cuts at: 0 1369 2022
Size: 1369 653
Bsp1286I G_dGCh'C
Cuts at: 0 894 2022
Size: 894 1128
BspHI T'CATG_A
Cuts at: 0 1598 2022
Size: 1598 424
BsrBI CCG'CTC
Cuts at: 0 759 2022
Size: 759 1263
BsrDI GCAATG_nn'
Cuts at: 0 264 1542 2022
Size: 264 1278 480
Fragments arranged by size:
1278 480 264
BsrGI T'GTAC_A
Cuts at: 0 920 2022
Size: 920 1102
BssSI C'ACGA_G
Cuts at: 0 1847 2022
Size: 1847 175
BstEII G'GTnAC_C
Cuts at: 0 910 2022
Size: 910 1112
BstXI CCAn_nnnn'nTGG
Cuts at: 0 204 1032 2022
Size: 204 828 990
Fragments arranged by size:
990 828 204
BstYI r'GATC_y
Cuts at: 0 645 1059 1571 2022
Size: 645 414 512 451
Fragments arranged by size:
645 512 451 414
BtgI C'CryG_G
Cuts at: 0 48 269 2022
Size: 48 221 1753
Fragments arranged by size:
1753 221 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 538 2022
Size: 538 1484
BtsI GCAGTG_nn'
Cuts at: 0 734 739 1405 2022
Size: 734 5 666 617
Fragments arranged by size:
734 666 617 5
DraIII CAC_nnn'GTG
Cuts at: 0 348 2022
Size: 348 1674
EaeI y'GGCC_r
Cuts at: 0 266 549 1692 2022
Size: 266 283 1143 330
Fragments arranged by size:
1143 330 283 266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 833 1361 1534 1549 1913 2022
Size: 760 73 528 173 15 364 109
Fragments arranged by size:
760 528 364 173 109 73 15
EcoO109I rG'GnC_Cy
Cuts at: 0 763 890 891 1092 2022
Size: 763 127 1 201 930
Fragments arranged by size:
930 763 201 127 1
EcoRV GAT'ATC
Cuts at: 0 1590 2022
Size: 1590 432
HaeII r_GCGC'y
Cuts at: 0 429 2017 2022
Size: 429 1588 5
Fragments arranged by size:
1588 429 5
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 1069 1101 1948 1980 2022
Size: 343 32 694 32 847 32 42
Fragments arranged by size:
847 694 343 42 32 32 32
HincII GTy'rAC
Cuts at: 0 378 2022
Size: 378 1644
KpnI G_GTAC'C
Cuts at: 0 1799 2022
Size: 1799 223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 2022
Size: 467 1555
MscI TGG'CCA
Cuts at: 0 551 1694 2022
Size: 551 1143 328
Fragments arranged by size:
1143 551 328
MslI CAynn'nnrTG
Cuts at: 0 202 1285 2022
Size: 202 1083 737
Fragments arranged by size:
1083 737 202
MspA1I CmG'CkG
Cuts at: 0 221 271 456 663 2022
Size: 221 50 185 207 1359
Fragments arranged by size:
1359 221 207 185 50
NspI r_CATG'y
Cuts at: 0 278 297 312 450 1482 2022
Size: 278 19 15 138 1032 540
Fragments arranged by size:
1032 540 278 138 19 15
PflMI CCAn_nnn'nTGG
Cuts at: 0 666 1011 2022
Size: 666 345 1011
Fragments arranged by size:
1011 666 345
PpuMI rG'GwC_Cy
Cuts at: 0 763 1092 2022
Size: 763 329 930
Fragments arranged by size:
930 763 329
PspOMI G'GGCC_C
Cuts at: 0 890 2022
Size: 890 1132
PstI C_TGCA'G
Cuts at: 0 141 777 899 990 2022
Size: 141 636 122 91 1032
Fragments arranged by size:
1032 636 141 122 91
PvuII CAG'CTG
Cuts at: 0 456 663 2022
Size: 456 207 1359
Fragments arranged by size:
1359 456 207
SacII CC_GC'GG
Cuts at: 0 272 2022
Size: 272 1750
SalI G'TCGA_C
Cuts at: 0 376 2022
Size: 376 1646
SanDI GG'GwC_CC
Cuts at: 0 763 2022
Size: 763 1259
ScaI AGT'ACT
Cuts at: 0 868 2022
Size: 868 1154
SexAI A'CCwGG_T
Cuts at: 0 1872 2022
Size: 1872 150
SfcI C'TryA_G
Cuts at: 0 137 451 773 895 986 1674 2022
Size: 137 314 322 122 91 688 348
Fragments arranged by size:
688 348 322 314 137 122 91
SmaI CCC'GGG
Cuts at: 0 10 2022
Size: 10 2012
SmlI C'TyrA_G
Cuts at: 0 157 529 2022
Size: 157 372 1493
Fragments arranged by size:
1493 372 157
SspI AAT'ATT
Cuts at: 0 1647 2022
Size: 1647 375
StuI AGG'CCT
Cuts at: 0 1808 2022
Size: 1808 214
StyI C'CwwG_G
Cuts at: 0 1025 1652 2022
Size: 1025 627 370
Fragments arranged by size:
1025 627 370
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1206 1992 2022
Size: 1206 786 30
Fragments arranged by size:
1206 786 30
TatI w'GTAC_w
Cuts at: 0 866 920 2022
Size: 866 54 1102
Fragments arranged by size:
1102 866 54
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 345 2022
Size: 345 1677
XmaI C'CCGG_G
Cuts at: 0 8 2022
Size: 8 2014
ZraI GAC'GTC
Cuts at: 0 499 2022
Size: 499 1523
Enzymes that do cut:
AatII AccI Acc65I AcuI AhdI AlfI AlwNI ApaI
AseI AvaI BanI BanII BbsI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspHI
BsrBI BsrDI BsrGI BssSI BstEII BstXI BstYI BtgI
BtgZI BtsI DraIII EaeI Eco57MI EcoO109I EcoRV HaeII
Hin4I HincII KpnI MmeI MscI MslI MspA1I NspI
PflMI PpuMI PspOMI PstI PvuII SacII SalI SanDI
ScaI SexAI SfcI SmaI SmlI SspI StuI StyI
TaqII TatI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AflIII AgeI AleI AloI
ApaLI ApoI AscI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BclI BfrBI BmtI Bpu10I BsaAI
BsaWI BsiEI BsiHKAI BsiWI BsmBI BspEI BspMI BsrFI
BssHII BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI
DrdI EagI EarI EciI EcoICRI EcoNI EcoRI FalI
FseI FspI FspAI HindIII HpaI KasI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SapI
SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SwaI TstI Tth111I XbaI XhoI XmnI