Restriction map for
pFN21AE4183
(Linear) (Six-base) MAPSORT of: KIEE4183.seq Check: 8005 from: 1 to: 1965
KIEE4183.orf
With 182 enzymes: SgfI *
March 22, 2012 17:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1442 1642 1676 1965
Size: 1442 200 34 289
Fragments arranged by size:
1442 289 200 34
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 867 961 1965
Size: 867 94 1004
Fragments arranged by size:
1004 867 94
AfeI AGC'GCT
Cuts at: 0 178 661 1865 1965
Size: 178 483 1204 100
Fragments arranged by size:
1204 483 178 100
AflIII A'CryG_T
Cuts at: 0 842 1965
Size: 842 1123
AleI CACnn'nnGTG
Cuts at: 0 598 1965
Size: 598 1367
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 661 1965
Size: 661 1304
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 199 231 1570 1602 1912 1944 1965
Size: 199 32 1339 32 310 32 21
Fragments arranged by size:
1339 310 199 32 32 32 21
AlwNI CAG_nnn'CTG
Cuts at: 0 918 1198 1965
Size: 918 280 767
Fragments arranged by size:
918 767 280
AvaI C'yCGr_G
Cuts at: 0 35 262 630 1965
Size: 35 227 368 1335
Fragments arranged by size:
1335 368 227 35
BamHI G'GATC_C
Cuts at: 0 923 1286 1965
Size: 923 363 679
Fragments arranged by size:
923 679 363
BanI G'GyrC_C
Cuts at: 0 612 850 1119 1372 1965
Size: 612 238 269 253 593
Fragments arranged by size:
612 593 269 253 238
BanII G_rGCy'C
Cuts at: 0 1036 1346 1718 1965
Size: 1036 310 372 247
Fragments arranged by size:
1036 372 310 247
BbeI G_GCGC'C
Cuts at: 0 854 1965
Size: 854 1111
BbsI GAAGACnn'nnnn_
Cuts at: 0 543 864 873 1663 1965
Size: 543 321 9 790 302
Fragments arranged by size:
790 543 321 302 9
BciVI GTATCCnnnnn_n'
Cuts at: 0 1099 1965
Size: 1099 866
BfrBI ATG'CAT
Cuts at: 0 1318 1965
Size: 1318 647
BglI GCCn_nnn'nGGC
Cuts at: 0 1619 1965
Size: 1619 346
BglII A'GATC_T
Cuts at: 0 1142 1965
Size: 1142 823
BlpI GC'TnA_GC
Cuts at: 0 487 1717 1965
Size: 487 1230 248
Fragments arranged by size:
1230 487 248
Bme1580I G_kGCm'C
Cuts at: 0 1263 1375 1965
Size: 1263 112 590
Fragments arranged by size:
1263 590 112
BmgBI CAC'GTC
Cuts at: 0 789 843 1965
Size: 789 54 1122
Fragments arranged by size:
1122 789 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 970 1111 1469 1965
Size: 970 141 358 496
Fragments arranged by size:
970 496 358 141
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 348 681 1050 1767 1965
Size: 348 333 369 717 198
Fragments arranged by size:
717 369 348 333 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 193 1381 1755 1965
Size: 180 13 1188 374 210
Fragments arranged by size:
1188 374 210 180 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1185 1965
Size: 1185 780
BsaI GGTCTCn'nnnn_
Cuts at: 0 1797 1959 1965
Size: 1797 162 6
Fragments arranged by size:
1797 162 6
BsaAI yAC'GTr
Cuts at: 0 561 1965
Size: 561 1404
BsaBI GATnn'nnATC
Cuts at: 0 1729 1965
Size: 1729 236
BsaHI Gr'CG_yC
Cuts at: 0 851 1172 1965
Size: 851 321 793
Fragments arranged by size:
851 793 321
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 199 229 634 664 1103 1133 1965
Size: 199 30 405 30 439 30 832
Fragments arranged by size:
832 439 405 199 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 415 1026 1336 1399 1965
Size: 415 611 310 63 566
Fragments arranged by size:
611 566 415 310 63
BseYI C'CCAG_C
Cuts at: 0 195 745 1860 1965
Size: 195 550 1115 105
Fragments arranged by size:
1115 550 195 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 1309 1423 1965
Size: 292 1017 114 542
Fragments arranged by size:
1017 542 292 114
BsiEI CG_ry'CG
Cuts at: 0 8 95 255 1965
Size: 8 87 160 1710
Fragments arranged by size:
1710 160 87 8
BsiHKAI G_wGCw'C
Cuts at: 0 284 1391 1718 1965
Size: 284 1107 327 247
Fragments arranged by size:
1107 327 284 247
BsmI GAATG_Cn'
Cuts at: 0 1320 1965
Size: 1320 645
BsmBI CGTCTCn'nnnn_
Cuts at: 0 601 1965
Size: 601 1364
Bsp1286I G_dGCh'C
Cuts at: 0 284 1036 1263 1346 1375 1391 1718 1965
Size: 284 752 227 83 29 16 327 247
Fragments arranged by size:
752 327 284 247 227 83 29 16
BspHI T'CATG_A
Cuts at: 0 701 773 824 1965
Size: 701 72 51 1141
Fragments arranged by size:
1141 701 72 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1331 1442 1642 1676 1691 1965
Size: 1331 111 200 34 15 274
Fragments arranged by size:
1331 274 200 111 34 15
BsrBI CCG'CTC
Cuts at: 0 435 1965
Size: 435 1530
BsrDI GCAATG_nn'
Cuts at: 0 1181 1947 1965
Size: 1181 766 18
Fragments arranged by size:
1181 766 18
BsrFI r'CCGG_y
Cuts at: 0 60 1965
Size: 60 1905
BssSI C'ACGA_G
Cuts at: 0 1522 1965
Size: 1522 443
BstBI TT'CG_AA
Cuts at: 0 1312 1965
Size: 1312 653
BstXI CCAn_nnnn'nTGG
Cuts at: 0 84 540 603 1965
Size: 84 456 63 1362
Fragments arranged by size:
1362 456 84 63
BstYI r'GATC_y
Cuts at: 0 887 923 1142 1286 1965
Size: 887 36 219 144 679
Fragments arranged by size:
887 679 219 144 36
BtgI C'CryG_G
Cuts at: 0 713 986 1965
Size: 713 273 979
Fragments arranged by size:
979 713 273
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 561 1965
Size: 561 1404
BtsI GCAGTG_nn'
Cuts at: 0 1440 1746 1965
Size: 1440 306 219
Fragments arranged by size:
1440 306 219
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 120 155 1965
Size: 120 35 1810
Fragments arranged by size:
1810 120 35
DraIII CAC_nnn'GTG
Cuts at: 0 695 1965
Size: 695 1270
EaeI y'GGCC_r
Cuts at: 0 5 44 65 92 794 989 1040 1295
Size: 5 39 21 27 702 195 51 255
Cuts at: 1295 1888 1965
Size: 593 77
Fragments arranged by size:
702 593 255 195 77 51 39 27
21 5
EagI C'GGCC_G
Cuts at: 0 5 92 1965
Size: 5 87 1873
Fragments arranged by size:
1873 87 5
EarI CTCTTCn'nnn_
Cuts at: 0 739 937 979 1376 1965
Size: 739 198 42 397 589
Fragments arranged by size:
739 589 397 198 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 159 484 1947 1965
Size: 159 325 1463 18
Fragments arranged by size:
1463 325 159 18
EcoICRI GAG'CTC
Cuts at: 0 1716 1965
Size: 1716 249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 193 867 961 1381 1755 1965
Size: 180 13 674 94 420 374 210
Fragments arranged by size:
674 420 374 210 180 94 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1351 1965
Size: 1351 614
EcoO109I rG'GnC_Cy
Cuts at: 0 258 915 1909 1965
Size: 258 657 994 56
Fragments arranged by size:
994 657 258 56
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 234 261 1965
Size: 234 27 1704
Fragments arranged by size:
1704 234 27
FspI TGC'GCA
Cuts at: 0 1702 1965
Size: 1702 263
HaeII r_GCGC'y
Cuts at: 0 180 663 854 1360 1867 1965
Size: 180 483 191 506 507 98
Fragments arranged by size:
507 506 483 191 180 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 439 471 829 861 1410 1442 1965
Size: 439 32 358 32 549 32 523
Fragments arranged by size:
549 523 439 358 32 32 32
KasI G'GCGC_C
Cuts at: 0 850 1965
Size: 850 1115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1235 1965
Size: 1235 730
MscI TGG'CCA
Cuts at: 0 67 796 991 1297 1965
Size: 67 729 195 306 668
Fragments arranged by size:
729 668 306 195 67
MslI CAynn'nnrTG
Cuts at: 0 598 787 1012 1965
Size: 598 189 225 953
Fragments arranged by size:
953 598 225 189
MspA1I CmG'CkG
Cuts at: 0 275 1965
Size: 275 1690
NarI GG'CG_CC
Cuts at: 0 851 1965
Size: 851 1114
NcoI C'CATG_G
Cuts at: 0 986 1965
Size: 986 979
NsiI A_TGCA'T
Cuts at: 0 1320 1965
Size: 1320 645
NspI r_CATG'y
Cuts at: 0 1710 1965
Size: 1710 255
PasI CC'CwG_GG
Cuts at: 0 912 1965
Size: 912 1053
PflMI CCAn_nnn'nTGG
Cuts at: 0 53 1965
Size: 53 1912
PfoI T'CCnGG_A
Cuts at: 0 371 1965
Size: 371 1594
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 199 231 1965
Size: 199 32 1734
Fragments arranged by size:
1734 199 32
PpuMI rG'GwC_Cy
Cuts at: 0 258 1965
Size: 258 1707
SacI G_AGCT'C
Cuts at: 0 1718 1965
Size: 1718 247
SanDI GG'GwC_CC
Cuts at: 0 258 1965
Size: 258 1707
SapI GCTCTTCn'nnn_
Cuts at: 0 739 937 1965
Size: 739 198 1028
Fragments arranged by size:
1028 739 198
ScaI AGT'ACT
Cuts at: 0 244 1965
Size: 244 1721
SfoI GGC'GCC
Cuts at: 0 852 1965
Size: 852 1113
SmaI CCC'GGG
Cuts at: 0 37 632 1965
Size: 37 595 1333
Fragments arranged by size:
1333 595 37
SmlI C'TyrA_G
Cuts at: 0 1164 1965
Size: 1164 801
SphI G_CATG'C
Cuts at: 0 1710 1965
Size: 1710 255
SrfI GCCC'GGGC
Cuts at: 0 37 1965
Size: 37 1928
StuI AGG'CCT
Cuts at: 0 1622 1965
Size: 1622 343
StyI C'CwwG_G
Cuts at: 0 236 986 1965
Size: 236 750 979
Fragments arranged by size:
979 750 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 576 805 1631 1965
Size: 576 229 826 334
Fragments arranged by size:
826 576 334 229
TatI w'GTAC_w
Cuts at: 0 242 404 1965
Size: 242 162 1561
Fragments arranged by size:
1561 242 162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 634 666 679 711 1103 1135 1581 1613
Size: 634 32 13 32 392 32 446 32
Cuts at: 1613 1908 1940 1965
Size: 295 32 25
Fragments arranged by size:
634 446 392 295 32 32 32 32
32 25 13
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1098 1290 1965
Size: 1098 192 675
Fragments arranged by size:
1098 675 192
XmaI C'CCGG_G
Cuts at: 0 35 630 1965
Size: 35 595 1335
Fragments arranged by size:
1335 595 35
XmnI GAAnn'nnTTC
Cuts at: 0 214 1144 1965
Size: 214 930 821
Fragments arranged by size:
930 821 214
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AloI AlwNI
AvaI BamHI BanI BanII BbeI BbsI BciVI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NarI NcoI NsiI
NspI PasI PflMI PfoI PpiI PpuMI SacI SanDI
SapI ScaI SfoI SmaI SmlI SphI SrfI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbvCI
BcgI BclI BmtI Bpu10I BsaWI BsiWI BspEI BsrGI
BssHII BstAPI BstEII BstZ17I Bsu36I ClaI DraI DrdI
EcoRI EcoRV FseI FspAI HincII HindIII HpaI KpnI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
PacI PciI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SbfI
SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI SspI
SwaI Tth111I XbaI XhoI ZraI