Restriction map for
pFN21AE4181
(Linear) (Six-base) MAPSORT of: KIEE4181.seq Check: 4738 from: 1 to: 1959
KIEE4181.orf
With 182 enzymes: SgfI *
March 22, 2012 17:32 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 1046 1959
Size: 742 304 913
Fragments arranged by size:
913 742 304
AflIII A'CryG_T
Cuts at: 0 528 1959
Size: 528 1431
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 754 786 1959
Size: 754 32 1173
Fragments arranged by size:
1173 754 32
AlwNI CAG_nnn'CTG
Cuts at: 0 124 127 148 518 1398 1959
Size: 124 3 21 370 880 561
Fragments arranged by size:
880 561 370 124 21 3
ApaI G_GGCC'C
Cuts at: 0 81 849 1959
Size: 81 768 1110
Fragments arranged by size:
1110 768 81
ApoI r'AATT_y
Cuts at: 0 862 1786 1959
Size: 862 924 173
Fragments arranged by size:
924 862 173
AvaI C'yCGr_G
Cuts at: 0 74 80 853 1959
Size: 74 6 773 1106
Fragments arranged by size:
1106 773 74 6
BanII G_rGCy'C
Cuts at: 0 31 81 181 849 907 1798 1959
Size: 31 50 100 668 58 891 161
Fragments arranged by size:
891 668 161 100 58 50 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 845 1959
Size: 845 1114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1599 1633 1959
Size: 1599 34 326
Fragments arranged by size:
1599 326 34
BfrBI ATG'CAT
Cuts at: 0 1540 1959
Size: 1540 419
BglII A'GATC_T
Cuts at: 0 462 1959
Size: 462 1497
Bme1580I G_kGCm'C
Cuts at: 0 81 849 1091 1261 1959
Size: 81 768 242 170 698
Fragments arranged by size:
768 698 242 170 81
BmrI ACTGGGnnnn_n'
Cuts at: 0 323 579 1264 1441 1959
Size: 323 256 685 177 518
Fragments arranged by size:
685 518 323 256 177
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 116 1959
Size: 116 1843
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 891 1096 1356 1959
Size: 90 801 205 260 603
Fragments arranged by size:
801 603 260 205 90
Bpu10I CC'TnA_GC
Cuts at: 0 30 1959
Size: 30 1929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1765 1959
Size: 1765 194
BsaI GGTCTCn'nnnn_
Cuts at: 0 556 602 1959
Size: 556 46 1357
Fragments arranged by size:
1357 556 46
BsaWI w'CCGG_w
Cuts at: 0 1355 1959
Size: 1355 604
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 400 430 754 781 784 811 1213 1243
Size: 400 30 324 27 3 27 402 30
Cuts at: 1243 1867 1897 1959
Size: 624 30 62
Fragments arranged by size:
624 402 400 324 62 30 30 30
27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 264 267 355 382 385 397 400 403
Size: 264 3 88 27 3 12 3 3
Cuts at: 403 412 421 775 802 874 1891 1959
Size: 9 9 354 27 72 1017 68
Fragments arranged by size:
1017 354 264 88 72 68 27 27
12 9 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 676 1180 1271 1959
Size: 676 504 91 688
Fragments arranged by size:
688 676 504 91
BsiHKAI G_wGCw'C
Cuts at: 0 31 907 1798 1959
Size: 31 876 891 161
Fragments arranged by size:
891 876 161 31
BsmI GAATG_Cn'
Cuts at: 0 1749 1959
Size: 1749 210
Bsp1286I G_dGCh'C
Cuts at: 0 31 81 181 849 907 1091 1261 1798
Size: 31 50 100 668 58 184 170 537
Cuts at: 1798 1959
Size: 161
Fragments arranged by size:
668 537 184 170 161 100 58 50
31
BspEI T'CCGG_A
Cuts at: 0 1355 1959
Size: 1355 604
BspHI T'CATG_A
Cuts at: 0 1208 1959
Size: 1208 751
BsrBI CCG'CTC
Cuts at: 0 87 1959
Size: 87 1872
BsrDI GCAATG_nn'
Cuts at: 0 17 309 654 1296 1634 1652 1959
Size: 17 292 345 642 338 18 307
Fragments arranged by size:
642 345 338 307 292 18 17
BsrFI r'CCGG_y
Cuts at: 0 290 1959
Size: 290 1669
BstAPI GCAn_nnn'nTGC
Cuts at: 0 124 1027 1959
Size: 124 903 932
Fragments arranged by size:
932 903 124
BstEII G'GTnAC_C
Cuts at: 0 1557 1959
Size: 1557 402
BstYI r'GATC_y
Cuts at: 0 347 462 1008 1358 1394 1959
Size: 347 115 546 350 36 565
Fragments arranged by size:
565 546 350 347 115 36
Bsu36I CC'TnA_GG
Cuts at: 0 352 473 1959
Size: 352 121 1486
Fragments arranged by size:
1486 352 121
BtgI C'CryG_G
Cuts at: 0 438 1959
Size: 438 1521
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1925 1959
Size: 1925 34
DraI TTT'AAA
Cuts at: 0 867 1959
Size: 867 1092
DraIII CAC_nnn'GTG
Cuts at: 0 1027 1959
Size: 1027 932
EaeI y'GGCC_r
Cuts at: 0 137 1959
Size: 137 1822
EarI CTCTTCn'nnn_
Cuts at: 0 389 398 718 827 1198 1459 1959
Size: 389 9 320 109 371 261 500
Fragments arranged by size:
500 389 371 320 261 109 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1516 1959
Size: 1516 443
EcoICRI GAG'CTC
Cuts at: 0 29 905 1796 1959
Size: 29 876 891 163
Fragments arranged by size:
891 876 163 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 742 891 1046 1096 1356 1959
Size: 90 652 149 155 50 260 603
Fragments arranged by size:
652 603 260 155 149 90 50
EcoO109I rG'GnC_Cy
Cuts at: 0 206 792 845 846 1622 1959
Size: 206 586 53 1 776 337
Fragments arranged by size:
776 586 337 206 53 1
EcoRV GAT'ATC
Cuts at: 0 750 1959
Size: 750 1209
FspI TGC'GCA
Cuts at: 0 646 1959
Size: 646 1313
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 894 926 1213 1245 1959
Size: 894 32 287 32 714
Fragments arranged by size:
894 714 287 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 1959
Size: 226 1733
MslI CAynn'nnrTG
Cuts at: 0 443 1545 1959
Size: 443 1102 414
Fragments arranged by size:
1102 443 414
MspA1I CmG'CkG
Cuts at: 0 115 124 136 145 264 1959
Size: 115 9 12 9 119 1695
Fragments arranged by size:
1695 119 115 12 9 9
NcoI C'CATG_G
Cuts at: 0 438 1959
Size: 438 1521
NsiI A_TGCA'T
Cuts at: 0 1542 1959
Size: 1542 417
NspI r_CATG'y
Cuts at: 0 51 1425 1959
Size: 51 1374 534
Fragments arranged by size:
1374 534 51
PasI CC'CwG_GG
Cuts at: 0 1812 1959
Size: 1812 147
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 754 786 1959
Size: 754 32 1173
Fragments arranged by size:
1173 754 32
PpuMI rG'GwC_Cy
Cuts at: 0 1622 1959
Size: 1622 337
PshAI GACnn'nnGTC
Cuts at: 0 264 1959
Size: 264 1695
PspOMI G'GGCC_C
Cuts at: 0 77 845 1959
Size: 77 768 1114
Fragments arranged by size:
1114 768 77
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 150 182 1959
Size: 150 32 1777
Fragments arranged by size:
1777 150 32
PstI C_TGCA'G
Cuts at: 0 120 1394 1959
Size: 120 1274 565
Fragments arranged by size:
1274 565 120
PvuII CAG'CTG
Cuts at: 0 115 124 145 1959
Size: 115 9 21 1814
Fragments arranged by size:
1814 115 21 9
SacI G_AGCT'C
Cuts at: 0 31 907 1798 1959
Size: 31 876 891 161
Fragments arranged by size:
891 876 161 31
SanDI GG'GwC_CC
Cuts at: 0 1622 1959
Size: 1622 337
ScaI AGT'ACT
Cuts at: 0 631 1959
Size: 631 1328
SfcI C'TryA_G
Cuts at: 0 116 423 495 510 1390 1601 1959
Size: 116 307 72 15 880 211 358
Fragments arranged by size:
880 358 307 211 116 72 15
SmaI CCC'GGG
Cuts at: 0 76 82 1959
Size: 76 6 1877
Fragments arranged by size:
1877 76 6
SmlI C'TyrA_G
Cuts at: 0 1780 1959
Size: 1780 179
SphI G_CATG'C
Cuts at: 0 1425 1959
Size: 1425 534
SrfI GCCC'GGGC
Cuts at: 0 76 1959
Size: 76 1883
SspI AAT'ATT
Cuts at: 0 1097 1959
Size: 1097 862
StuI AGG'CCT
Cuts at: 0 551 1388 1959
Size: 551 837 571
Fragments arranged by size:
837 571 551
StyI C'CwwG_G
Cuts at: 0 438 1959
Size: 438 1521
TatI w'GTAC_w
Cuts at: 0 629 1959
Size: 629 1330
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1849 1867 1881 1899 1959
Size: 1849 18 14 18 60
Fragments arranged by size:
1849 60 18 18 14
Tth111I GACn'n_nGTC
Cuts at: 0 1225 1959
Size: 1225 734
XbaI T'CTAG_A
Cuts at: 0 1833 1959
Size: 1833 126
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1541 1569 1959
Size: 1541 28 390
Fragments arranged by size:
1541 390 28
XmaI C'CCGG_G
Cuts at: 0 74 80 1959
Size: 74 6 1879
Fragments arranged by size:
1879 74 6
Enzymes that do cut:
AcuI AflIII AloI AlwNI ApaI ApoI AvaI BanII
BbsI BcgI BfrBI BglII Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BstAPI
BstEII BstYI Bsu36I BtgI CspCI DraI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRV FspI Hin4I
MmeI MslI MspA1I NcoI NsiI NspI PasI PpiI
PpuMI PshAI PspOMI PsrI PstI PvuII SacI SanDI
ScaI SfcI SmaI SmlI SphI SrfI SspI StuI
StyI TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BciVI BclI BglI
BlpI BmgBI BmtI BsaAI BsaBI BsaHI BsgI BsiEI
BsiWI BsmBI BspMI BsrGI BssHII BssSI BstBI BstXI
BstZ17I BtgZI BtsI ClaI DrdI EagI EcoNI EcoRI
FalI FseI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PciI PflMI PfoI PmeI
PmlI PsiI PspXI PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SwaI TaqII XhoI XmnI ZraI