Restriction map for
pFN21AE4179
(Linear) (Six-base) MAPSORT of: KIEE4179.seq Check: 1946 from: 1 to: 1932
KIEE4179.orf
With 182 enzymes: SgfI *
March 22, 2012 17:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 221 1932
Size: 221 1711
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 1932
Size: 144 1788
AfeI AGC'GCT
Cuts at: 0 454 1627 1932
Size: 454 1173 305
Fragments arranged by size:
1173 454 305
AlwNI CAG_nnn'CTG
Cuts at: 0 63 246 480 1932
Size: 63 183 234 1452
Fragments arranged by size:
1452 234 183 63
ApaI G_GGCC'C
Cuts at: 0 168 502 1248 1501 1932
Size: 168 334 746 253 431
Fragments arranged by size:
746 431 334 253 168
ApaLI G'TGCA_C
Cuts at: 0 1083 1183 1932
Size: 1083 100 749
Fragments arranged by size:
1083 749 100
ApoI r'AATT_y
Cuts at: 0 115 1932
Size: 115 1817
AvaI C'yCGr_G
Cuts at: 0 194 574 711 737 940 1434 1932
Size: 194 380 137 26 203 494 498
Fragments arranged by size:
498 494 380 203 194 137 26
BanI G'GyrC_C
Cuts at: 0 550 570 1527 1537 1553 1639 1758 1932
Size: 550 20 957 10 16 86 119 174
Fragments arranged by size:
957 550 174 119 86 20 16 10
BanII G_rGCy'C
Cuts at: 0 168 350 502 521 623 718 832 941
Size: 168 182 152 19 102 95 114 109
Cuts at: 941 995 1126 1248 1386 1501 1610 1932
Size: 54 131 122 138 115 109 322
Fragments arranged by size:
322 182 168 152 138 131 122 115
114 109 109 102 95 54 19
BbeI G_GCGC'C
Cuts at: 0 1643 1932
Size: 1643 289
BbvCI CC'TCA_GC
Cuts at: 0 535 1932
Size: 535 1397
BglI GCCn_nnn'nGGC
Cuts at: 0 409 1130 1932
Size: 409 721 802
Fragments arranged by size:
802 721 409
Bme1580I G_kGCm'C
Cuts at: 0 168 502 555 575 870 1076 1087 1187
Size: 168 334 53 20 295 206 11 100
Cuts at: 1187 1226 1248 1374 1501 1540 1556 1932
Size: 39 22 126 127 39 16 376
Fragments arranged by size:
376 334 295 206 168 127 126 100
53 39 39 22 20 16 11
BmgBI CAC'GTC
Cuts at: 0 90 561 1932
Size: 90 471 1371
Fragments arranged by size:
1371 471 90
BmrI ACTGGGnnnn_n'
Cuts at: 0 556 598 1932
Size: 556 42 1334
Fragments arranged by size:
1334 556 42
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1629 1932
Size: 1629 303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 132 328 365 535 679 1857 1932
Size: 118 14 196 37 170 144 1178 75
Fragments arranged by size:
1178 196 170 144 118 75 37 14
Bpu10I CC'TnA_GC
Cuts at: 0 535 1932
Size: 535 1397
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1720 1932
Size: 1720 212
BsaI GGTCTCn'nnnn_
Cuts at: 0 94 1932
Size: 94 1838
BsaAI yAC'GTr
Cuts at: 0 219 1932
Size: 219 1713
BsaHI Gr'CG_yC
Cuts at: 0 781 1640 1932
Size: 781 859 292
Fragments arranged by size:
859 781 292
BsaWI w'CCGG_w
Cuts at: 0 824 1932
Size: 824 1108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 970 1000 1702 1732 1932
Size: 970 30 702 30 200
Fragments arranged by size:
970 702 200 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 901 985 988 991 994 997 1519 1723
Size: 901 84 3 3 3 3 522 204
Cuts at: 1723 1932
Size: 209
Fragments arranged by size:
901 522 209 204 84 3 3 3
3
BseYI C'CCAG_C
Cuts at: 0 1068 1366 1725 1926 1932
Size: 1068 298 359 201 6
Fragments arranged by size:
1068 359 298 201 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 1372 1932
Size: 199 1173 560
Fragments arranged by size:
1173 560 199
BsiEI CG_ry'CG
Cuts at: 0 449 1666 1680 1932
Size: 449 1217 14 252
Fragments arranged by size:
1217 449 252 14
BsiHKAI G_wGCw'C
Cuts at: 0 148 521 995 1087 1187 1932
Size: 148 373 474 92 100 745
Fragments arranged by size:
745 474 373 148 100 92
Bsp1286I G_dGCh'C
Cuts at: 0 148 168 350 502 521 555 575 623
Size: 148 20 182 152 19 34 20 48
Cuts at: 623 718 832 870 941 995 1076 1087 1126
Size: 95 114 38 71 54 81 11 39
Cuts at: 1126 1187 1226 1248 1374 1386 1501 1540 1556
Size: 61 39 22 126 12 115 39 16
Cuts at: 1556 1610 1932
Size: 54 322
Fragments arranged by size:
322 182 152 148 126 115 114 95
81 71 61 54 54 48 39 39
39 38 34 22 20 20 19 16
12 11
BspEI T'CCGG_A
Cuts at: 0 824 1932
Size: 824 1108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 221 318 332 1349 1500 1932
Size: 221 97 14 1017 151 432
Fragments arranged by size:
1017 432 221 151 97 14
BsrBI CCG'CTC
Cuts at: 0 798 1841 1932
Size: 798 1043 91
Fragments arranged by size:
1043 798 91
BsrFI r'CCGG_y
Cuts at: 0 49 232 1932
Size: 49 183 1700
Fragments arranged by size:
1700 183 49
BsrGI T'GTAC_A
Cuts at: 0 646 1022 1427 1932
Size: 646 376 405 505
Fragments arranged by size:
646 505 405 376
BssHII G'CGCG_C
Cuts at: 0 38 66 199 415 1932
Size: 38 28 133 216 1517
Fragments arranged by size:
1517 216 133 38 28
BssSI C'ACGA_G
Cuts at: 0 724 1932
Size: 724 1208
BstAPI GCAn_nnn'nTGC
Cuts at: 0 246 480 1932
Size: 246 234 1452
Fragments arranged by size:
1452 246 234
Bsu36I CC'TnA_GG
Cuts at: 0 1502 1932
Size: 1502 430
BtgI C'CryG_G
Cuts at: 0 284 503 1088 1378 1416 1532 1700 1932
Size: 284 219 585 290 38 116 168 232
Fragments arranged by size:
585 290 284 232 219 168 116 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 515 1932
Size: 515 1417
BtsI GCAGTG_nn'
Cuts at: 0 263 1063 1140 1571 1862 1932
Size: 263 800 77 431 291 70
Fragments arranged by size:
800 431 291 263 77 70
DraIII CAC_nnn'GTG
Cuts at: 0 1080 1932
Size: 1080 852
DrdI GACnn_nn'nnGTC
Cuts at: 0 1489 1932
Size: 1489 443
EaeI y'GGCC_r
Cuts at: 0 446 1151 1283 1663 1697 1868 1932
Size: 446 705 132 380 34 171 64
Fragments arranged by size:
705 446 380 171 132 64 34
EagI C'GGCC_G
Cuts at: 0 446 1663 1932
Size: 446 1217 269
Fragments arranged by size:
1217 446 269
EarI CTCTTCn'nnn_
Cuts at: 0 944 950 962 1932
Size: 944 6 12 970
Fragments arranged by size:
970 944 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1478 1932
Size: 1478 454
EcoICRI GAG'CTC
Cuts at: 0 519 993 1932
Size: 519 474 939
Fragments arranged by size:
939 519 474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 132 144 328 365 535 679 1857
Size: 118 14 12 184 37 170 144 1178
Cuts at: 1857 1932
Size: 75
Fragments arranged by size:
1178 184 170 144 118 75 37 14
12
EcoO109I rG'GnC_Cy
Cuts at: 0 60 164 165 339 499 747 762 1293
Size: 60 104 1 174 160 248 15 531
Cuts at: 1293 1498 1932
Size: 205 434
Fragments arranged by size:
531 434 248 205 174 160 104 60
15 1
EcoRI G'AATT_C
Cuts at: 0 115 1932
Size: 115 1817
FspI TGC'GCA
Cuts at: 0 1854 1932
Size: 1854 78
HaeII r_GCGC'y
Cuts at: 0 246 360 372 456 636 1580 1629 1643
Size: 246 114 12 84 180 944 49 14
Cuts at: 1643 1915 1932
Size: 272 17
Fragments arranged by size:
944 272 246 180 114 84 49 17
14 12
KasI G'GCGC_C
Cuts at: 0 1639 1932
Size: 1639 293
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 941 1884 1932
Size: 941 943 48
Fragments arranged by size:
943 941 48
MslI CAynn'nnrTG
Cuts at: 0 1564 1932
Size: 1564 368
MspA1I CmG'CkG
Cuts at: 0 375 505 1034 1090 1355 1380 1418 1668
Size: 375 130 529 56 265 25 38 250
Cuts at: 1668 1866 1932
Size: 198 66
Fragments arranged by size:
529 375 265 250 198 130 66 56
38 25
NaeI GCC'GGC
Cuts at: 0 234 1932
Size: 234 1698
NarI GG'CG_CC
Cuts at: 0 1640 1932
Size: 1640 292
NcoI C'CATG_G
Cuts at: 0 284 1532 1932
Size: 284 1248 400
Fragments arranged by size:
1248 400 284
NgoMIV G'CCGG_C
Cuts at: 0 232 1932
Size: 232 1700
NotI GC'GGCC_GC
Cuts at: 0 1663 1932
Size: 1663 269
PasI CC'CwG_GG
Cuts at: 0 342 1829 1932
Size: 342 1487 103
Fragments arranged by size:
1487 342 103
PflMI CCAn_nnn'nTGG
Cuts at: 0 1005 1932
Size: 1005 927
PfoI T'CCnGG_A
Cuts at: 0 512 1478 1932
Size: 512 966 454
Fragments arranged by size:
966 512 454
PmlI CAC'GTG
Cuts at: 0 219 1932
Size: 219 1713
PshAI GACnn'nnGTC
Cuts at: 0 1343 1932
Size: 1343 589
PspOMI G'GGCC_C
Cuts at: 0 164 498 1244 1497 1932
Size: 164 334 746 253 435
Fragments arranged by size:
746 435 334 253 164
PstI C_TGCA'G
Cuts at: 0 242 329 1865 1932
Size: 242 87 1536 67
Fragments arranged by size:
1536 242 87 67
PvuII CAG'CTG
Cuts at: 0 375 1866 1932
Size: 375 1491 66
Fragments arranged by size:
1491 375 66
SacI G_AGCT'C
Cuts at: 0 521 995 1932
Size: 521 474 937
Fragments arranged by size:
937 521 474
SacII CC_GC'GG
Cuts at: 0 506 1091 1381 1419 1932
Size: 506 585 290 38 513
Fragments arranged by size:
585 513 506 290 38
SbfI CC_TGCA'GG
Cuts at: 0 329 1932
Size: 329 1603
SfcI C'TryA_G
Cuts at: 0 238 293 325 1861 1932
Size: 238 55 32 1536 71
Fragments arranged by size:
1536 238 71 55 32
SfoI GGC'GCC
Cuts at: 0 1641 1932
Size: 1641 291
SmaI CCC'GGG
Cuts at: 0 196 739 1932
Size: 196 543 1193
Fragments arranged by size:
1193 543 196
SmlI C'TyrA_G
Cuts at: 0 1735 1932
Size: 1735 197
StyI C'CwwG_G
Cuts at: 0 284 1532 1932
Size: 284 1248 400
Fragments arranged by size:
1248 400 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 118 199 538 1012 1102 1288 1545 1932
Size: 118 81 339 474 90 186 257 387
Fragments arranged by size:
474 387 339 257 186 118 90 81
TatI w'GTAC_w
Cuts at: 0 543 646 707 1022 1427 1932
Size: 543 103 61 315 405 505
Fragments arranged by size:
543 505 405 315 103 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 970 1002 1932
Size: 970 32 930
Fragments arranged by size:
970 930 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1065 1932
Size: 1065 867
XmaI C'CCGG_G
Cuts at: 0 194 737 1932
Size: 194 543 1195
Fragments arranged by size:
1195 543 194
Enzymes that do cut:
AarI AcuI AfeI AlwNI ApaI ApaLI ApoI AvaI
BanI BanII BbeI BbvCI BglI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI Bsu36I
BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII KasI
MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
PasI PflMI PfoI PmlI PshAI PspOMI PstI PvuII
SacI SacII SbfI SfcI SfoI SmaI SmlI StyI
TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BcgI BciVI BclI BfrBI BglII BlpI
BmtI BsaBI BsiWI BsmI BsmBI BspHI BsrDI BstBI
BstEII BstXI BstYI BstZ17I ClaI CspCI DraI EcoNI
EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MscI NdeI NheI NruI NsiI
NspI PacI PciI PmeI PpiI PpuMI PsiI PspXI
PsrI PvuI RsrII SalI SanDI SapI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XhoI XmnI ZraI