Restriction map for
pFN21AE4176
(Linear) (Six-base) MAPSORT of: KIEE4176.seq Check: 1692 from: 1 to: 1914
KIEE4176.orf
With 182 enzymes: SgfI *
March 22, 2012 17:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1398 1914
Size: 1398 516
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 178 652 995 1744 1914
Size: 3 175 474 343 749 170
Fragments arranged by size:
749 474 343 175 170 3
AfeI AGC'GCT
Cuts at: 0 571 1914
Size: 571 1343
AflIII A'CryG_T
Cuts at: 0 389 1914
Size: 389 1525
AlwNI CAG_nnn'CTG
Cuts at: 0 820 855 1005 1188 1413 1914
Size: 820 35 150 183 225 501
Fragments arranged by size:
820 501 225 183 150 35
ApaI G_GGCC'C
Cuts at: 0 253 472 1877 1914
Size: 253 219 1405 37
Fragments arranged by size:
1405 253 219 37
AvaI C'yCGr_G
Cuts at: 0 246 253 349 397 565 1059 1222 1914
Size: 246 7 96 48 168 494 163 692
Fragments arranged by size:
692 494 246 168 163 96 48 7
BanI G'GyrC_C
Cuts at: 0 300 510 642 1423 1438 1914
Size: 300 210 132 781 15 476
Fragments arranged by size:
781 476 300 210 132 15
BanII G_rGCy'C
Cuts at: 0 179 247 253 296 472 951 1070 1214
Size: 179 68 6 43 176 479 119 144
Cuts at: 1214 1520 1811 1877 1914
Size: 306 291 66 37
Fragments arranged by size:
479 306 291 179 176 144 119 68
66 43 37 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 1505 1914
Size: 1505 409
BbvCI CC'TCA_GC
Cuts at: 0 1150 1914
Size: 1150 764
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 709 743 1914
Size: 709 34 1171
Fragments arranged by size:
1171 709 34
BglI GCCn_nnn'nGGC
Cuts at: 0 401 1538 1748 1914
Size: 401 1137 210 166
Fragments arranged by size:
1137 401 210 166
BlpI GC'TnA_GC
Cuts at: 0 677 1069 1208 1914
Size: 677 392 139 706
Fragments arranged by size:
706 677 392 139
Bme1580I G_kGCm'C
Cuts at: 0 253 397 472 547 1428 1877 1914
Size: 253 144 75 75 881 449 37
Fragments arranged by size:
881 449 253 144 75 75 37
BmrI ACTGGGnnnn_n'
Cuts at: 0 311 1458 1914
Size: 311 1147 456
Fragments arranged by size:
1147 456 311
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1268 1914
Size: 1268 646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 1048 1627 1864 1914
Size: 670 378 579 237 50
Fragments arranged by size:
670 579 378 237 50
Bpu10I CC'TnA_GC
Cuts at: 0 694 940 1150 1914
Size: 694 246 210 764
Fragments arranged by size:
764 694 246 210
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1255 1810 1864 1914
Size: 1255 555 54 50
Fragments arranged by size:
1255 555 54 50
BsaHI Gr'CG_yC
Cuts at: 0 1856 1914
Size: 1856 58
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 526 556 577 607 1021 1051 1537 1567
Size: 526 30 21 30 414 30 486 30
Cuts at: 1567 1914
Size: 347
Fragments arranged by size:
526 486 414 347 30 30 30 30
21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 547 883 1914
Size: 547 336 1031
Fragments arranged by size:
1031 547 336
BseYI C'CCAG_C
Cuts at: 0 304 723 855 1413 1472 1536 1914
Size: 304 419 132 558 59 64 378
Fragments arranged by size:
558 419 378 304 132 64 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 794 1121 1914
Size: 794 327 793
Fragments arranged by size:
794 793 327
BsiEI CG_ry'CG
Cuts at: 0 827 1527 1533 1914
Size: 827 700 6 381
Fragments arranged by size:
827 700 381 6
BsiHKAI G_wGCw'C
Cuts at: 0 626 1070 1914
Size: 626 444 844
Fragments arranged by size:
844 626 444
Bsp1286I G_dGCh'C
Cuts at: 0 179 247 253 296 397 472 547 626
Size: 179 68 6 43 101 75 75 79
Cuts at: 626 951 1070 1214 1428 1520 1811 1877 1914
Size: 325 119 144 214 92 291 66 37
Fragments arranged by size:
325 291 214 179 144 119 101 92
79 75 75 68 66 43 37 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 917 1398 1914
Size: 917 481 516
Fragments arranged by size:
917 516 481
BsrBI CCG'CTC
Cuts at: 0 564 789 896 1914
Size: 564 225 107 1018
Fragments arranged by size:
1018 564 225 107
BsrDI GCAATG_nn'
Cuts at: 0 734 1914
Size: 734 1180
BsrFI r'CCGG_y
Cuts at: 0 54 133 524 803 1532 1914
Size: 54 79 391 279 729 382
Fragments arranged by size:
729 391 382 279 79 54
BssHII G'CGCG_C
Cuts at: 0 101 308 660 662 1914
Size: 101 207 352 2 1252
Fragments arranged by size:
1252 352 207 101 2
BssSI C'ACGA_G
Cuts at: 0 475 634 1914
Size: 475 159 1280
Fragments arranged by size:
1280 475 159
BstAPI GCAn_nnn'nTGC
Cuts at: 0 820 1005 1914
Size: 820 185 909
Fragments arranged by size:
909 820 185
BstEII G'GTnAC_C
Cuts at: 0 904 1914
Size: 904 1010
BtgI C'CryG_G
Cuts at: 0 355 362 495 627 701 1121 1527 1637
Size: 355 7 133 132 74 420 406 110
Cuts at: 1637 1914
Size: 277
Fragments arranged by size:
420 406 355 277 133 132 110 74
7
BtsI GCAGTG_nn'
Cuts at: 0 15 1109 1573 1914
Size: 15 1094 464 341
Fragments arranged by size:
1094 464 341 15
EaeI y'GGCC_r
Cuts at: 0 72 109 559 630 824 1118 1493 1530
Size: 72 37 450 71 194 294 375 37
Cuts at: 1530 1914
Size: 384
Fragments arranged by size:
450 384 375 294 194 72 71 37
37
EagI C'GGCC_G
Cuts at: 0 824 1530 1914
Size: 824 706 384
Fragments arranged by size:
824 706 384
EarI CTCTTCn'nnn_
Cuts at: 0 365 1816 1900 1914
Size: 365 1451 84 14
Fragments arranged by size:
1451 365 84 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 152 226 1914
Size: 152 74 1688
Fragments arranged by size:
1688 152 74
EcoICRI GAG'CTC
Cuts at: 0 1068 1914
Size: 1068 846
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 178 652 670 995 1048 1627 1744
Size: 3 175 474 18 325 53 579 117
Cuts at: 1744 1864 1914
Size: 120 50
Fragments arranged by size:
579 474 325 175 120 117 53 50
18 3
EcoO109I rG'GnC_Cy
Cuts at: 0 250 969 1874 1914
Size: 250 719 905 40
Fragments arranged by size:
905 719 250 40
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1851 1914
Size: 1851 63
HaeII r_GCGC'y
Cuts at: 0 573 701 1914
Size: 573 128 1213
Fragments arranged by size:
1213 573 128
HincII GTy'rAC
Cuts at: 0 1134 1914
Size: 1134 780
MluI A'CGCG_T
Cuts at: 0 389 1914
Size: 389 1525
MslI CAynn'nnrTG
Cuts at: 0 1897 1914
Size: 1897 17
MspA1I CmG'CkG
Cuts at: 0 23 158 165 364 497 519 629 648
Size: 23 135 7 199 133 22 110 19
Cuts at: 648 1149 1326 1529 1914
Size: 501 177 203 385
Fragments arranged by size:
501 385 203 199 177 135 133 110
23 22 19 7
NaeI GCC'GGC
Cuts at: 0 135 526 805 1534 1914
Size: 135 391 279 729 380
Fragments arranged by size:
729 391 380 279 135
NgoMIV G'CCGG_C
Cuts at: 0 133 524 803 1532 1914
Size: 133 391 279 729 382
Fragments arranged by size:
729 391 382 279 133
NotI GC'GGCC_GC
Cuts at: 0 824 1914
Size: 824 1090
NspI r_CATG'y
Cuts at: 0 1116 1914
Size: 1116 798
PasI CC'CwG_GG
Cuts at: 0 900 1045 1256 1914
Size: 900 145 211 658
Fragments arranged by size:
900 658 211 145
PflMI CCAn_nnn'nTGG
Cuts at: 0 639 855 1914
Size: 639 216 1059
Fragments arranged by size:
1059 639 216
PfoI T'CCnGG_A
Cuts at: 0 47 323 1841 1914
Size: 47 276 1518 73
Fragments arranged by size:
1518 276 73 47
PpuMI rG'GwC_Cy
Cuts at: 0 969 1914
Size: 969 945
PspOMI G'GGCC_C
Cuts at: 0 249 468 1873 1914
Size: 249 219 1405 41
Fragments arranged by size:
1405 249 219 41
PspXI vC'TCGA_Gb
Cuts at: 0 565 1914
Size: 565 1349
PstI C_TGCA'G
Cuts at: 0 233 836 1001 1325 1568 1914
Size: 233 603 165 324 243 346
Fragments arranged by size:
603 346 324 243 233 165
PvuII CAG'CTG
Cuts at: 0 23 1149 1326 1914
Size: 23 1126 177 588
Fragments arranged by size:
1126 588 177 23
SacI G_AGCT'C
Cuts at: 0 1070 1914
Size: 1070 844
SacII CC_GC'GG
Cuts at: 0 365 498 630 1530 1914
Size: 365 133 132 900 384
Fragments arranged by size:
900 384 365 133 132
SapI GCTCTTCn'nnn_
Cuts at: 0 365 1816 1914
Size: 365 1451 98
Fragments arranged by size:
1451 365 98
SbfI CC_TGCA'GG
Cuts at: 0 836 1001 1914
Size: 836 165 913
Fragments arranged by size:
913 836 165
SfcI C'TryA_G
Cuts at: 0 229 832 997 1321 1564 1914
Size: 229 603 165 324 243 350
Fragments arranged by size:
603 350 324 243 229 165
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1538 1914
Size: 1538 376
SmaI CCC'GGG
Cuts at: 0 255 1914
Size: 255 1659
SmlI C'TyrA_G
Cuts at: 0 565 1222 1270 1825 1879 1914
Size: 565 657 48 555 54 35
Fragments arranged by size:
657 565 555 54 48 35
StuI AGG'CCT
Cuts at: 0 607 1729 1914
Size: 607 1122 185
Fragments arranged by size:
1122 607 185
StyI C'CwwG_G
Cuts at: 0 1673 1914
Size: 1673 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 318 715 1025 1411 1448 1831 1914
Size: 318 397 310 386 37 383 83
Fragments arranged by size:
397 386 383 318 310 83 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 202 234 526 558 577 609 1021 1053
Size: 202 32 292 32 19 32 412 32
Cuts at: 1053 1914
Size: 861
Fragments arranged by size:
861 412 292 202 32 32 32 32
19
Tth111I GACn'n_nGTC
Cuts at: 0 985 1914
Size: 985 929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 753 1914
Size: 753 1161
XhoI C'TCGA_G
Cuts at: 0 565 1222 1914
Size: 565 657 692
Fragments arranged by size:
692 657 565
XmaI C'CCGG_G
Cuts at: 0 253 1914
Size: 253 1661
Enzymes that do cut:
AarI AcuI AfeI AflIII AlwNI ApaI AvaI BanI
BanII BbsI BbvCI BcgI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstEII BtgI BtsI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII HincII
MluI MslI MspA1I NaeI NgoMIV NotI NspI PasI
PflMI PfoI PpuMI PspOMI PspXI PstI PvuII SacI
SacII SapI SbfI SfcI SfiI SmaI SmlI StuI
StyI TaqII TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BciVI BclI BfrBI BglII BmgBI
BmtI BsaI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI
BspEI BspHI BsrGI BstBI BstXI BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EcoNI EcoRI
EcoRV FseI FspI FspAI Hin4I HindIII HpaI KasI
KpnI MfeI MmeI MscI NarI NcoI NdeI NheI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PsrI PvuI RsrII SalI SanDI ScaI SexAI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TatI XbaI XmnI ZraI