Restriction map for
pFN21AE4174
(Linear) (Six-base) MAPSORT of: KIEE4174.seq Check: 3208 from: 1 to: 1902
KIEE4174.orf
With 182 enzymes: SgfI *
March 22, 2012 17:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1644 1902
Size: 1644 258
AccI GT'mk_AC
Cuts at: 0 412 1902
Size: 412 1490
Acc65I G'GTAC_C
Cuts at: 0 232 781 1902
Size: 232 549 1121
Fragments arranged by size:
1121 549 232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 1218 1743 1902
Size: 433 785 525 159
Fragments arranged by size:
785 525 433 159
AlwNI CAG_nnn'CTG
Cuts at: 0 1485 1902
Size: 1485 417
ApoI r'AATT_y
Cuts at: 0 577 601 724 1902
Size: 577 24 123 1178
Fragments arranged by size:
1178 577 123 24
AseI AT'TA_AT
Cuts at: 0 647 1902
Size: 647 1255
AvaI C'yCGr_G
Cuts at: 0 1505 1765 1801 1856 1865 1902
Size: 1505 260 36 55 9 37
Fragments arranged by size:
1505 260 55 37 36 9
BanI G'GyrC_C
Cuts at: 0 3 232 781 1644 1902
Size: 3 229 549 863 258
Fragments arranged by size:
863 549 258 229 3
BanII G_rGCy'C
Cuts at: 0 107 1259 1772 1902
Size: 107 1152 513 130
Fragments arranged by size:
1152 513 130 107
BbeI G_GCGC'C
Cuts at: 0 1648 1902
Size: 1648 254
BbsI GAAGACnn'nnnn_
Cuts at: 0 1124 1351 1902
Size: 1124 227 551
Fragments arranged by size:
1124 551 227
BbvCI CC'TCA_GC
Cuts at: 0 1495 1902
Size: 1495 407
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 923 957 1902
Size: 923 34 945
Fragments arranged by size:
945 923 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1190 1902
Size: 1190 712
BglII A'GATC_T
Cuts at: 0 756 1902
Size: 756 1146
BlpI GC'TnA_GC
Cuts at: 0 1531 1902
Size: 1531 371
Bme1580I G_kGCm'C
Cuts at: 0 1322 1902
Size: 1322 580
BmgBI CAC'GTC
Cuts at: 0 1593 1902
Size: 1593 309
BmtI G_CTAG'C
Cuts at: 0 1382 1902
Size: 1382 520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1327 1375 1902
Size: 1327 48 527
Fragments arranged by size:
1327 527 48
Bpu10I CC'TnA_GC
Cuts at: 0 127 994 1240 1495 1902
Size: 127 867 246 255 407
Fragments arranged by size:
867 407 255 246 127
BsaI GGTCTCn'nnnn_
Cuts at: 0 1010 1902
Size: 1010 892
BsaAI yAC'GTr
Cuts at: 0 1317 1548 1902
Size: 1317 231 354
Fragments arranged by size:
1317 354 231
BsaHI Gr'CG_yC
Cuts at: 0 969 1348 1645 1676 1902
Size: 969 379 297 31 226
Fragments arranged by size:
969 379 297 226 31
BsaWI w'CCGG_w
Cuts at: 0 1411 1902
Size: 1411 491
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1306 1336 1902
Size: 1306 30 566
Fragments arranged by size:
1306 566 30
BseYI C'CCAG_C
Cuts at: 0 1566 1902
Size: 1566 336
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 776 1812 1902
Size: 495 281 1036 90
Fragments arranged by size:
1036 495 281 90
BsiEI CG_ry'CG
Cuts at: 0 1278 1388 1398 1625 1902
Size: 1278 110 10 227 277
Fragments arranged by size:
1278 277 227 110 10
BsiHKAI G_wGCw'C
Cuts at: 0 1598 1669 1772 1902
Size: 1598 71 103 130
Fragments arranged by size:
1598 130 103 71
BsmI GAATG_Cn'
Cuts at: 0 96 697 1902
Size: 96 601 1205
Fragments arranged by size:
1205 601 96
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1683 1902
Size: 1683 219
Bsp1286I G_dGCh'C
Cuts at: 0 107 1259 1322 1598 1669 1772 1902
Size: 107 1152 63 276 71 103 130
Fragments arranged by size:
1152 276 130 107 103 71 63
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1644 1902
Size: 1644 258
BsrDI GCAATG_nn'
Cuts at: 0 126 371 716 907 1902
Size: 126 245 345 191 995
Fragments arranged by size:
995 345 245 191 126
BsrFI r'CCGG_y
Cuts at: 0 1108 1624 1902
Size: 1108 516 278
Fragments arranged by size:
1108 516 278
BssHII G'CGCG_C
Cuts at: 0 1363 1902
Size: 1363 539
BssSI C'ACGA_G
Cuts at: 0 1258 1473 1902
Size: 1258 215 429
Fragments arranged by size:
1258 429 215
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1166 1485 1754 1902
Size: 1166 319 269 148
Fragments arranged by size:
1166 319 269 148
BstEII G'GTnAC_C
Cuts at: 0 1407 1902
Size: 1407 495
BstXI CCAn_nnnn'nTGG
Cuts at: 0 177 1902
Size: 177 1725
BstYI r'GATC_y
Cuts at: 0 333 452 756 1902
Size: 333 119 304 1146
Fragments arranged by size:
1146 333 304 119
BstZ17I GTA'TAC
Cuts at: 0 413 1902
Size: 413 1489
BtgI C'CryG_G
Cuts at: 0 1268 1601 1902
Size: 1268 333 301
Fragments arranged by size:
1268 333 301
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1407 1902
Size: 1407 495
BtsI GCAGTG_nn'
Cuts at: 0 288 483 1515 1667 1902
Size: 288 195 1032 152 235
Fragments arranged by size:
1032 288 235 195 152
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 354 389 1902
Size: 354 35 1513
Fragments arranged by size:
1513 354 35
EaeI y'GGCC_r
Cuts at: 0 362 1205 1275 1395 1463 1626 1692 1902
Size: 362 843 70 120 68 163 66 210
Fragments arranged by size:
843 362 210 163 120 70 68 66
EagI C'GGCC_G
Cuts at: 0 1275 1395 1902
Size: 1275 120 507
Fragments arranged by size:
1275 507 120
EcoICRI GAG'CTC
Cuts at: 0 1770 1902
Size: 1770 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 1218 1327 1375 1743 1902
Size: 433 785 109 48 368 159
Fragments arranged by size:
785 433 368 159 109 48
EcoRV GAT'ATC
Cuts at: 0 42 345 1902
Size: 42 303 1557
Fragments arranged by size:
1557 303 42
HaeII r_GCGC'y
Cuts at: 0 1648 1687 1902
Size: 1648 39 215
Fragments arranged by size:
1648 215 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 318 350 444 476 938 970 1406 1438
Size: 318 32 94 32 462 32 436 32
Cuts at: 1438 1902
Size: 464
Fragments arranged by size:
464 462 436 318 94 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1039 1902
Size: 1039 863
KasI G'GCGC_C
Cuts at: 0 1644 1902
Size: 1644 258
KpnI G_GTAC'C
Cuts at: 0 236 785 1902
Size: 236 549 1117
Fragments arranged by size:
1117 549 236
MscI TGG'CCA
Cuts at: 0 364 1207 1902
Size: 364 843 695
Fragments arranged by size:
843 695 364
MspA1I CmG'CkG
Cuts at: 0 1270 1566 1650 1902
Size: 1270 296 84 252
Fragments arranged by size:
1270 296 252 84
NarI GG'CG_CC
Cuts at: 0 1645 1902
Size: 1645 257
NdeI CA'TA_TG
Cuts at: 0 378 1902
Size: 378 1524
NheI G'CTAG_C
Cuts at: 0 1378 1902
Size: 1378 524
NotI GC'GGCC_GC
Cuts at: 0 1275 1902
Size: 1275 627
NspI r_CATG'y
Cuts at: 0 519 1153 1902
Size: 519 634 749
Fragments arranged by size:
749 634 519
PflMI CCAn_nnn'nTGG
Cuts at: 0 1334 1902
Size: 1334 568
PsiI TTA'TAA
Cuts at: 0 466 1902
Size: 466 1436
PstI C_TGCA'G
Cuts at: 0 756 1171 1655 1902
Size: 756 415 484 247
Fragments arranged by size:
756 484 415 247
PvuII CAG'CTG
Cuts at: 0 1566 1902
Size: 1566 336
SacI G_AGCT'C
Cuts at: 0 1772 1902
Size: 1772 130
SacII CC_GC'GG
Cuts at: 0 1271 1902
Size: 1271 631
SexAI A'CCwGG_T
Cuts at: 0 800 1703 1902
Size: 800 903 199
Fragments arranged by size:
903 800 199
SfcI C'TryA_G
Cuts at: 0 752 1167 1611 1651 1902
Size: 752 415 444 40 251
Fragments arranged by size:
752 444 415 251 40
SfoI GGC'GCC
Cuts at: 0 1646 1902
Size: 1646 256
SmaI CCC'GGG
Cuts at: 0 1507 1867 1902
Size: 1507 360 35
Fragments arranged by size:
1507 360 35
SphI G_CATG'C
Cuts at: 0 1153 1902
Size: 1153 749
SspI AAT'ATT
Cuts at: 0 531 771 1902
Size: 531 240 1131
Fragments arranged by size:
1131 531 240
StuI AGG'CCT
Cuts at: 0 359 977 1902
Size: 359 618 925
Fragments arranged by size:
925 618 359
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 21 1902
Size: 21 1881
TatI w'GTAC_w
Cuts at: 0 1736 1902
Size: 1736 166
Tth111I GACn'n_nGTC
Cuts at: 0 223 802 1902
Size: 223 579 1100
Fragments arranged by size:
1100 579 223
XmaI C'CCGG_G
Cuts at: 0 1505 1865 1902
Size: 1505 360 37
Fragments arranged by size:
1505 360 37
Enzymes that do cut:
AarI AccI Acc65I AcuI AlwNI ApoI AseI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BglII
BlpI Bme1580I BmgBI BmtI BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrDI BsrFI BssHII BssSI BstAPI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI
EaeI EagI EcoICRI Eco57MI EcoRV HaeII Hin4I HindIII
KasI KpnI MscI MspA1I NarI NdeI NheI NotI
NspI PflMI PsiI PstI PvuII SacI SacII SexAI
SfcI SfoI SmaI SphI SspI StuI TaqII TatI
Tth111I XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BclI BfrBI BglI BmrI BplI BpuEI BsaBI
BseRI BsiWI BspEI BspHI BsrBI BsrGI BstBI Bsu36I
ClaI DraI DraIII DrdI EarI EciI EcoNI EcoO109I
EcoRI FalI FseI FspI FspAI HincII HpaI MfeI
MluI MmeI MslI NaeI NcoI NgoMIV NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PvuI RsrII SalI SanDI
SapI SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI
SpeI SrfI StyI SwaI TstI XbaI XcmI XhoI
XmnI ZraI