Restriction map for
pFN21AE4173
(Linear) (Six-base) MAPSORT of: KIEE4173.seq Check: 4534 from: 1 to: 1902
KIEE4173.orf
With 182 enzymes: SgfI *
March 22, 2012 17:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 184 1307 1902
Size: 184 1123 595
Fragments arranged by size:
1123 595 184
AatII G_ACGT'C
Cuts at: 0 1400 1902
Size: 1400 502
AccI GT'mk_AC
Cuts at: 0 1686 1902
Size: 1686 216
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 1902
Size: 226 1676
AfeI AGC'GCT
Cuts at: 0 776 1902
Size: 776 1126
AflIII A'CryG_T
Cuts at: 0 350 1902
Size: 350 1552
AgeI A'CCGG_T
Cuts at: 0 1208 1902
Size: 1208 694
AleI CACnn'nnGTG
Cuts at: 0 1225 1902
Size: 1225 677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1243 1284 1586 1902
Size: 1243 41 302 316
Fragments arranged by size:
1243 316 302 41
AlwNI CAG_nnn'CTG
Cuts at: 0 252 1090 1341 1902
Size: 252 838 251 561
Fragments arranged by size:
838 561 252 251
ApaI G_GGCC'C
Cuts at: 0 23 34 1148 1902
Size: 23 11 1114 754
Fragments arranged by size:
1114 754 23 11
ApaLI G'TGCA_C
Cuts at: 0 465 1902
Size: 465 1437
ApoI r'AATT_y
Cuts at: 0 1033 1902
Size: 1033 869
AvaI C'yCGr_G
Cuts at: 0 33 51 1902
Size: 33 18 1851
Fragments arranged by size:
1851 33 18
BanI G'GyrC_C
Cuts at: 0 1295 1659 1902
Size: 1295 364 243
Fragments arranged by size:
1295 364 243
BanII G_rGCy'C
Cuts at: 0 23 34 40 224 485 1148 1649 1800
Size: 23 11 6 184 261 663 501 151
Cuts at: 1800 1902
Size: 102
Fragments arranged by size:
663 501 261 184 151 102 23 11
6
BbsI GAAGACnn'nnnn_
Cuts at: 0 100 1464 1774 1902
Size: 100 1364 310 128
Fragments arranged by size:
1364 310 128 100
BbvCI CC'TCA_GC
Cuts at: 0 901 1558 1725 1902
Size: 901 657 167 177
Fragments arranged by size:
901 657 177 167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 115 149 1449 1483 1902
Size: 115 34 1300 34 419
Fragments arranged by size:
1300 419 115 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 149 634 916 1902
Size: 149 485 282 986
Fragments arranged by size:
986 485 282 149
Bme1580I G_kGCm'C
Cuts at: 0 23 34 76 458 469 564 1148 1902
Size: 23 11 42 382 11 95 584 754
Fragments arranged by size:
754 584 382 95 42 23 11 11
BmgBI CAC'GTC
Cuts at: 0 327 453 1075 1425 1902
Size: 327 126 622 350 477
Fragments arranged by size:
622 477 350 327 126
BmrI ACTGGGnnnn_n'
Cuts at: 0 1156 1902
Size: 1156 746
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 855 1464 1902
Size: 855 609 438
Fragments arranged by size:
855 609 438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 328 631 1069 1537 1663 1902
Size: 46 282 303 438 468 126 239
Fragments arranged by size:
468 438 303 282 239 126 46
Bpu10I CC'TnA_GC
Cuts at: 0 264 901 1122 1558 1725 1902
Size: 264 637 221 436 167 177
Fragments arranged by size:
637 436 264 221 177 167
BsaI GGTCTCn'nnnn_
Cuts at: 0 325 1343 1902
Size: 325 1018 559
Fragments arranged by size:
1018 559 325
BsaAI yAC'GTr
Cuts at: 0 87 338 353 591 1902
Size: 87 251 15 238 1311
Fragments arranged by size:
1311 251 238 87 15
BsaHI Gr'CG_yC
Cuts at: 0 1397 1902
Size: 1397 505
BsaWI w'CCGG_w
Cuts at: 0 1208 1902
Size: 1208 694
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 322 352 1141 1171 1600 1630 1636 1666
Size: 322 30 789 30 429 30 6 30
Cuts at: 1666 1902
Size: 236
Fragments arranged by size:
789 429 322 236 30 30 30 30
6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 703 727 730 882 1902
Size: 703 24 3 152 1020
Fragments arranged by size:
1020 703 152 24 3
BseYI C'CCAG_C
Cuts at: 0 214 1065 1535 1793 1902
Size: 214 851 470 258 109
Fragments arranged by size:
851 470 258 214 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 333 457 613 814 1189 1327 1387
Size: 109 224 124 156 201 375 138 60
Cuts at: 1387 1447 1902
Size: 60 455
Fragments arranged by size:
455 375 224 201 156 138 124 109
60 60
BsiEI CG_ry'CG
Cuts at: 0 161 168 979 1902
Size: 161 7 811 923
Fragments arranged by size:
923 811 161 7
BsiHKAI G_wGCw'C
Cuts at: 0 40 402 469 1268 1445 1902
Size: 40 362 67 799 177 457
Fragments arranged by size:
799 457 362 177 67 40
BsmI GAATG_Cn'
Cuts at: 0 1575 1902
Size: 1575 327
BsmBI CGTCTCn'nnnn_
Cuts at: 0 382 1388 1902
Size: 382 1006 514
Fragments arranged by size:
1006 514 382
Bsp1286I G_dGCh'C
Cuts at: 0 23 34 40 76 224 402 458 469
Size: 23 11 6 36 148 178 56 11
Cuts at: 469 485 564 1148 1268 1445 1649 1800 1902
Size: 16 79 584 120 177 204 151 102
Fragments arranged by size:
584 204 178 177 151 148 120 102
79 56 36 23 16 11 11 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 184 861 1307 1902
Size: 184 677 446 595
Fragments arranged by size:
677 595 446 184
BsrBI CCG'CTC
Cuts at: 0 606 1616 1638 1902
Size: 606 1010 22 264
Fragments arranged by size:
1010 606 264 22
BsrDI GCAATG_nn'
Cuts at: 0 1619 1902
Size: 1619 283
BsrFI r'CCGG_y
Cuts at: 0 136 228 236 1208 1292 1902
Size: 136 92 8 972 84 610
Fragments arranged by size:
972 610 136 92 84 8
BssHII G'CGCG_C
Cuts at: 0 371 373 412 1039 1902
Size: 371 2 39 627 863
Fragments arranged by size:
863 627 371 39 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 353 530 621 792 1090 1099 1902
Size: 353 177 91 171 298 9 803
Fragments arranged by size:
803 353 298 177 171 91 9
BstEII G'GTnAC_C
Cuts at: 0 1181 1902
Size: 1181 721
BstYI r'GATC_y
Cuts at: 0 1849 1902
Size: 1849 53
Bsu36I CC'TnA_GG
Cuts at: 0 988 1902
Size: 988 914
BtgI C'CryG_G
Cuts at: 0 680 1664 1902
Size: 680 984 238
Fragments arranged by size:
984 680 238
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 648 1886 1902
Size: 648 1238 16
Fragments arranged by size:
1238 648 16
BtsI GCAGTG_nn'
Cuts at: 0 564 1054 1149 1266 1902
Size: 564 490 95 117 636
Fragments arranged by size:
636 564 490 117 95
DraIII CAC_nnn'GTG
Cuts at: 0 570 1902
Size: 570 1332
EaeI y'GGCC_r
Cuts at: 0 150 165 626 647 848 908 976 1902
Size: 150 15 461 21 201 60 68 926
Fragments arranged by size:
926 461 201 150 68 60 21 15
EagI C'GGCC_G
Cuts at: 0 165 976 1902
Size: 165 811 926
Fragments arranged by size:
926 811 165
EarI CTCTTCn'nnn_
Cuts at: 0 742 803 1811 1902
Size: 742 61 1008 91
Fragments arranged by size:
1008 742 91 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1252 1396 1902
Size: 1252 144 506
Fragments arranged by size:
1252 506 144
EcoICRI GAG'CTC
Cuts at: 0 38 1902
Size: 38 1864
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 226 328 631 1069 1537 1663 1902
Size: 46 180 102 303 438 468 126 239
Fragments arranged by size:
468 438 303 239 180 126 102 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1728 1902
Size: 1728 174
EcoO109I rG'GnC_Cy
Cuts at: 0 20 30 211 636 1126 1144 1145 1902
Size: 20 10 181 425 490 18 1 757
Fragments arranged by size:
757 490 425 181 20 18 10 1
EcoRI G'AATT_C
Cuts at: 0 1033 1902
Size: 1033 869
HaeII r_GCGC'y
Cuts at: 0 102 707 778 1138 1902
Size: 102 605 71 360 764
Fragments arranged by size:
764 605 360 102 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 565 597 1660 1692 1756 1788 1902
Size: 565 32 1063 32 64 32 114
Fragments arranged by size:
1063 565 114 64 32 32 32
MscI TGG'CCA
Cuts at: 0 850 910 1902
Size: 850 60 992
Fragments arranged by size:
992 850 60
MslI CAynn'nnrTG
Cuts at: 0 1225 1902
Size: 1225 677
MspA1I CmG'CkG
Cuts at: 0 66 78 183 218 530 1087 1557 1793
Size: 66 12 105 35 312 557 470 236
Cuts at: 1793 1902
Size: 109
Fragments arranged by size:
557 470 312 236 109 105 66 35
12
NaeI GCC'GGC
Cuts at: 0 138 1902
Size: 138 1764
NcoI C'CATG_G
Cuts at: 0 680 1902
Size: 680 1222
NgoMIV G'CCGG_C
Cuts at: 0 136 1902
Size: 136 1766
NotI GC'GGCC_GC
Cuts at: 0 976 1902
Size: 976 926
NspI r_CATG'y
Cuts at: 0 408 546 750 1902
Size: 408 138 204 1152
Fragments arranged by size:
1152 408 204 138
PasI CC'CwG_GG
Cuts at: 0 432 1902
Size: 432 1470
PflMI CCAn_nnn'nTGG
Cuts at: 0 66 1902
Size: 66 1836
PfoI T'CCnGG_A
Cuts at: 0 926 1475 1740 1751 1902
Size: 926 549 265 11 151
Fragments arranged by size:
926 549 265 151 11
PmlI CAC'GTG
Cuts at: 0 87 353 1902
Size: 87 266 1549
Fragments arranged by size:
1549 266 87
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1795 1827 1902
Size: 1795 32 75
Fragments arranged by size:
1795 75 32
PpuMI rG'GwC_Cy
Cuts at: 0 211 1902
Size: 211 1691
PspOMI G'GGCC_C
Cuts at: 0 19 30 1144 1902
Size: 19 11 1114 758
Fragments arranged by size:
1114 758 19 11
PstI C_TGCA'G
Cuts at: 0 248 644 677 788 872 962 1304 1902
Size: 248 396 33 111 84 90 342 598
Fragments arranged by size:
598 396 342 248 111 90 84 33
PvuII CAG'CTG
Cuts at: 0 1087 1557 1793 1902
Size: 1087 470 236 109
Fragments arranged by size:
1087 470 236 109
SacI G_AGCT'C
Cuts at: 0 40 1902
Size: 40 1862
SanDI GG'GwC_CC
Cuts at: 0 211 1902
Size: 211 1691
SapI GCTCTTCn'nnn_
Cuts at: 0 1811 1902
Size: 1811 91
SbfI CC_TGCA'GG
Cuts at: 0 872 1304 1902
Size: 872 432 598
Fragments arranged by size:
872 598 432
SexAI A'CCwGG_T
Cuts at: 0 878 1328 1902
Size: 878 450 574
Fragments arranged by size:
878 574 450
SfcI C'TryA_G
Cuts at: 0 244 640 673 784 868 958 1300 1687
Size: 244 396 33 111 84 90 342 387
Cuts at: 1687 1902
Size: 215
Fragments arranged by size:
396 387 342 244 215 111 90 84
33
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 634 1902
Size: 634 1268
SgrAI Cr'CCGG_yG
Cuts at: 0 228 1902
Size: 228 1674
SphI G_CATG'C
Cuts at: 0 750 1902
Size: 750 1152
StuI AGG'CCT
Cuts at: 0 823 993 1902
Size: 823 170 909
Fragments arranged by size:
909 823 170
StyI C'CwwG_G
Cuts at: 0 680 1130 1223 1625 1902
Size: 680 450 93 402 277
Fragments arranged by size:
680 450 402 277 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 175 1902
Size: 175 1727
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1141 1173 1902
Size: 1141 32 729
Fragments arranged by size:
1141 729 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 303 1230 1902
Size: 303 927 672
Fragments arranged by size:
927 672 303
ZraI GAC'GTC
Cuts at: 0 1398 1902
Size: 1398 504
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AvaI BanI BanII
BbsI BbvCI BcgI BglI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssHII BstAPI BstEII BstYI Bsu36I
BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I MscI
MslI MspA1I NaeI NcoI NgoMIV NotI NspI PasI
PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII
SacI SanDI SapI SbfI SexAI SfcI SfiI SgrAI
SphI StuI StyI TaqII TstI XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AhdI AloI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BciVI BclI BfrBI BglII
BlpI BmtI BpuEI BsaBI BsiWI BspEI BspHI BsrGI
BssSI BstBI BstXI BstZ17I ClaI CspCI DraI DrdI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NarI NdeI NheI
NruI NsiI PacI PciI PmeI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI ScaI SfoI SgfI
SmaI SmlI SnaBI SpeI SrfI SspI SwaI TatI
Tth111I XbaI XhoI XmaI XmnI