Restriction map for
pFN21AE4168
(Linear) (Six-base) MAPSORT of: KIEE4168.seq Check: 8361 from: 1 to: 1860
KIEE4168.orf
With 182 enzymes: SgfI *
July 3, 2012 18:42 ..
AccI GT'mk_AC
Cuts at: 0 1454 1860
Size: 1454 406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 553 601 1860
Size: 544 9 48 1259
Fragments arranged by size:
1259 544 48 9
AfeI AGC'GCT
Cuts at: 0 92 1860
Size: 92 1768
AflIII A'CryG_T
Cuts at: 0 1084 1579 1860
Size: 1084 495 281
Fragments arranged by size:
1084 495 281
AhdI GACnn_n'nnGTC
Cuts at: 0 1103 1860
Size: 1103 757
AleI CACnn'nnGTG
Cuts at: 0 1016 1860
Size: 1016 844
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 140 172 1860
Size: 140 32 1688
Fragments arranged by size:
1688 140 32
AlwNI CAG_nnn'CTG
Cuts at: 0 288 1236 1704 1860
Size: 288 948 468 156
Fragments arranged by size:
948 468 288 156
ApoI r'AATT_y
Cuts at: 0 154 1860
Size: 154 1706
BamHI G'GATC_C
Cuts at: 0 1529 1860
Size: 1529 331
BanI G'GyrC_C
Cuts at: 0 1180 1852 1860
Size: 1180 672 8
Fragments arranged by size:
1180 672 8
BanII G_rGCy'C
Cuts at: 0 680 1860
Size: 680 1180
BbsI GAAGACnn'nnnn_
Cuts at: 0 1330 1860
Size: 1330 530
BbvCI CC'TCA_GC
Cuts at: 0 143 1860
Size: 143 1717
BciVI GTATCCnnnnn_n'
Cuts at: 0 708 1705 1860
Size: 708 997 155
Fragments arranged by size:
997 708 155
BclI T'GATC_A
Cuts at: 0 939 1860
Size: 939 921
BglI GCCn_nnn'nGGC
Cuts at: 0 828 1860
Size: 828 1032
BglII A'GATC_T
Cuts at: 0 617 1860
Size: 617 1243
BmgBI CAC'GTC
Cuts at: 0 1085 1860
Size: 1085 775
BmtI G_CTAG'C
Cuts at: 0 349 631 1860
Size: 349 282 1229
Fragments arranged by size:
1229 349 282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 340 996 1640 1860
Size: 310 30 656 644 220
Fragments arranged by size:
656 644 310 220 30
Bpu10I CC'TnA_GC
Cuts at: 0 143 1860
Size: 143 1717
BsaWI w'CCGG_w
Cuts at: 0 1526 1860
Size: 1526 334
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1046 1076 1613 1643 1860
Size: 1046 30 537 30 217
Fragments arranged by size:
1046 537 217 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 120 1860
Size: 120 1740
BseYI C'CCAG_C
Cuts at: 0 1176 1860
Size: 1176 684
BsiEI CG_ry'CG
Cuts at: 0 591 1860
Size: 591 1269
BsiHKAI G_wGCw'C
Cuts at: 0 680 1860
Size: 680 1180
BsmI GAATG_Cn'
Cuts at: 0 954 1860
Size: 954 906
Bsp1286I G_dGCh'C
Cuts at: 0 680 1860
Size: 680 1180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1604 1860
Size: 1604 256
BsrDI GCAATG_nn'
Cuts at: 0 212 965 1509 1860
Size: 212 753 544 351
Fragments arranged by size:
753 544 351 212
BsrGI T'GTAC_A
Cuts at: 0 440 1473 1860
Size: 440 1033 387
Fragments arranged by size:
1033 440 387
BstAPI GCAn_nnn'nTGC
Cuts at: 0 122 1173 1860
Size: 122 1051 687
Fragments arranged by size:
1051 687 122
BstXI CCAn_nnnn'nTGG
Cuts at: 0 999 1860
Size: 999 861
BstYI r'GATC_y
Cuts at: 0 435 617 1300 1529 1860
Size: 435 182 683 229 331
Fragments arranged by size:
683 435 331 229 182
BtgI C'CryG_G
Cuts at: 0 1184 1463 1860
Size: 1184 279 397
Fragments arranged by size:
1184 397 279
BtsI GCAGTG_nn'
Cuts at: 0 195 491 1860
Size: 195 296 1369
Fragments arranged by size:
1369 296 195
ClaI AT'CG_AT
Cuts at: 0 491 1860
Size: 491 1369
DraIII CAC_nnn'GTG
Cuts at: 0 261 1860
Size: 261 1599
EaeI y'GGCC_r
Cuts at: 0 1077 1605 1860
Size: 1077 528 255
Fragments arranged by size:
1077 528 255
EarI CTCTTCn'nnn_
Cuts at: 0 520 577 605 1832 1860
Size: 520 57 28 1227 28
Fragments arranged by size:
1227 520 57 28 28
EcoICRI GAG'CTC
Cuts at: 0 678 1860
Size: 678 1182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 340 544 553 601 996 1640 1860
Size: 310 30 204 9 48 395 644 220
Fragments arranged by size:
644 395 310 220 204 48 30 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 568 1860
Size: 568 1292
EcoO109I rG'GnC_Cy
Cuts at: 0 789 1860
Size: 789 1071
EcoRV GAT'ATC
Cuts at: 0 1349 1860
Size: 1349 511
HaeII r_GCGC'y
Cuts at: 0 94 1860
Size: 94 1766
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1089 1121 1613 1645 1860
Size: 1089 32 492 32 215
Fragments arranged by size:
1089 492 215 32 32
HincII GTy'rAC
Cuts at: 0 783 1860
Size: 783 1077
HindIII A'AGCT_T
Cuts at: 0 1483 1860
Size: 1483 377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 455 623 1860
Size: 353 102 168 1237
Fragments arranged by size:
1237 353 168 102
MscI TGG'CCA
Cuts at: 0 1607 1860
Size: 1607 253
MslI CAynn'nnrTG
Cuts at: 0 997 1016 1073 1860
Size: 997 19 57 787
Fragments arranged by size:
997 787 57 19
MspA1I CmG'CkG
Cuts at: 0 352 369 1860
Size: 352 17 1491
Fragments arranged by size:
1491 352 17
NcoI C'CATG_G
Cuts at: 0 1184 1463 1860
Size: 1184 279 397
Fragments arranged by size:
1184 397 279
NheI G'CTAG_C
Cuts at: 0 345 627 1860
Size: 345 282 1233
Fragments arranged by size:
1233 345 282
NspI r_CATG'y
Cuts at: 0 188 1072 1784 1811 1860
Size: 188 884 712 27 49
Fragments arranged by size:
884 712 188 49 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 669 1020 1464 1704 1860
Size: 669 351 444 240 156
Fragments arranged by size:
669 444 351 240 156
PfoI T'CCnGG_A
Cuts at: 0 335 407 1860
Size: 335 72 1453
Fragments arranged by size:
1453 335 72
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2 34 1860
Size: 2 32 1826
Fragments arranged by size:
1826 32 2
PstI C_TGCA'G
Cuts at: 0 1445 1860
Size: 1445 415
PvuII CAG'CTG
Cuts at: 0 369 1860
Size: 369 1491
SacI G_AGCT'C
Cuts at: 0 680 1860
Size: 680 1180
SapI GCTCTTCn'nnn_
Cuts at: 0 520 577 605 1860
Size: 520 57 28 1255
Fragments arranged by size:
1255 520 57 28
SfcI C'TryA_G
Cuts at: 0 364 1241 1441 1860
Size: 364 877 200 419
Fragments arranged by size:
877 419 364 200
SphI G_CATG'C
Cuts at: 0 188 1072 1784 1860
Size: 188 884 712 76
Fragments arranged by size:
884 712 188 76
StyI C'CwwG_G
Cuts at: 0 270 1184 1463 1860
Size: 270 914 279 397
Fragments arranged by size:
914 397 279 270
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1161 1168 1860
Size: 1161 7 692
Fragments arranged by size:
1161 692 7
TatI w'GTAC_w
Cuts at: 0 440 665 1473 1860
Size: 440 225 808 387
Fragments arranged by size:
808 440 387 225
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1338 1370 1860
Size: 1338 32 490
Fragments arranged by size:
1338 490 32
Tth111I GACn'n_nGTC
Cuts at: 0 79 1860
Size: 79 1781
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1615 1860
Size: 1615 245
XmnI GAAnn'nnTTC
Cuts at: 0 542 1860
Size: 542 1318
Enzymes that do cut:
AccI AcuI AfeI AflIII AhdI AleI AloI AlwNI
ApoI BamHI BanI BanII BbsI BbvCI BciVI BclI
BglI BglII BmgBI BmtI BpmI Bpu10I BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BsrGI BstAPI BstXI BstYI BtgI BtsI ClaI DraIII
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII
Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI
NheI NspI PflMI PfoI PpiI PstI PvuII SacI
SapI SfcI SphI StyI TaqII TatI TstI Tth111I
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AlfI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI
BcgI BfrBI BlpI Bme1580I BmrI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsgI BsiWI BsmBI BspEI BspHI
BsrBI BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI CspCI DraI DrdI EagI EciI EcoRI FalI
FseI FspI FspAI HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI
XmaI ZraI