Restriction map for
pFN21AE4165
(Linear) (Six-base) MAPSORT of: KIEE4165.seq Check: 7935 from: 1 to: 1845
KIEE4165.orf
With 182 enzymes: SgfI *
July 3, 2012 18:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1118 1845
Size: 1118 727
AatII G_ACGT'C
Cuts at: 0 308 1845
Size: 308 1537
AccI GT'mk_AC
Cuts at: 0 989 1845
Size: 989 856
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 337 487 507 1357 1845
Size: 246 91 150 20 850 488
Fragments arranged by size:
850 488 246 150 91 20
AflIII A'CryG_T
Cuts at: 0 1058 1845
Size: 1058 787
AleI CACnn'nnGTG
Cuts at: 0 907 1063 1845
Size: 907 156 782
Fragments arranged by size:
907 782 156
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 667 677 925 1845
Size: 667 10 248 920
Fragments arranged by size:
920 667 248 10
AlwNI CAG_nnn'CTG
Cuts at: 0 156 435 801 840 867 1110 1845
Size: 156 279 366 39 27 243 735
Fragments arranged by size:
735 366 279 243 156 39 27
ApaI G_GGCC'C
Cuts at: 0 61 67 105 1845
Size: 61 6 38 1740
Fragments arranged by size:
1740 61 38 6
ApaLI G'TGCA_C
Cuts at: 0 111 1845
Size: 111 1734
AvaI C'yCGr_G
Cuts at: 0 60 67 1392 1537 1826 1845
Size: 60 7 1325 145 289 19
Fragments arranged by size:
1325 289 145 60 19 7
BanI G'GyrC_C
Cuts at: 0 123 1473 1513 1845
Size: 123 1350 40 332
Fragments arranged by size:
1350 332 123 40
BanII G_rGCy'C
Cuts at: 0 61 67 105 1014 1031 1630 1845
Size: 61 6 38 909 17 599 215
Fragments arranged by size:
909 599 215 61 38 17 6
BbeI G_GCGC'C
Cuts at: 0 1477 1845
Size: 1477 368
BbsI GAAGACnn'nnnn_
Cuts at: 0 621 1845
Size: 621 1224
BglI GCCn_nnn'nGGC
Cuts at: 0 876 1845
Size: 876 969
BglII A'GATC_T
Cuts at: 0 1679 1845
Size: 1679 166
Bme1580I G_kGCm'C
Cuts at: 0 61 67 105 115 926 1845
Size: 61 6 38 10 811 919
Fragments arranged by size:
919 811 61 38 10 6
BmgBI CAC'GTC
Cuts at: 0 405 822 1575 1845
Size: 405 417 753 270
Fragments arranged by size:
753 417 405 270
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 865 1690 1845
Size: 637 228 825 155
Fragments arranged by size:
825 637 228 155
Bpu10I CC'TnA_GC
Cuts at: 0 173 1001 1845
Size: 173 828 844
Fragments arranged by size:
844 828 173
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 1845
Size: 91 1754
BsaI GGTCTCn'nnnn_
Cuts at: 0 481 1845
Size: 481 1364
BsaAI yAC'GTr
Cuts at: 0 738 1061 1092 1557 1845
Size: 738 323 31 465 288
Fragments arranged by size:
738 465 323 288 31
BsaBI GATnn'nnATC
Cuts at: 0 1684 1845
Size: 1684 161
BsaHI Gr'CG_yC
Cuts at: 0 305 1142 1474 1707 1845
Size: 305 837 332 233 138
Fragments arranged by size:
837 332 305 233 138
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 175 472 604 607 1845
Size: 175 297 132 3 1238
Fragments arranged by size:
1238 297 175 132 3
BseYI C'CCAG_C
Cuts at: 0 127 408 1360 1845
Size: 127 281 952 485
Fragments arranged by size:
952 485 281 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 418 814 1417 1759 1845
Size: 209 209 396 603 342 86
Fragments arranged by size:
603 396 342 209 209 86
BsiEI CG_ry'CG
Cuts at: 0 89 419 689 724 969 1260 1524 1845
Size: 89 330 270 35 245 291 264 321
Fragments arranged by size:
330 321 291 270 264 245 89 35
BsiHKAI G_wGCw'C
Cuts at: 0 115 743 1031 1845
Size: 115 628 288 814
Fragments arranged by size:
814 628 288 115
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1597 1714 1845
Size: 1597 117 131
Fragments arranged by size:
1597 131 117
Bsp1286I G_dGCh'C
Cuts at: 0 61 67 105 115 743 926 1014 1031
Size: 61 6 38 10 628 183 88 17
Cuts at: 1031 1630 1845
Size: 599 215
Fragments arranged by size:
628 599 215 183 88 61 38 17
10 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 499 1118 1781 1845
Size: 499 619 663 64
Fragments arranged by size:
663 619 499 64
BsrBI CCG'CTC
Cuts at: 0 762 971 1293 1767 1845
Size: 762 209 322 474 78
Fragments arranged by size:
762 474 322 209 78
BsrDI GCAATG_nn'
Cuts at: 0 1317 1845
Size: 1317 528
BsrFI r'CCGG_y
Cuts at: 0 277 647 1270 1516 1639 1654 1845
Size: 277 370 623 246 123 15 191
Fragments arranged by size:
623 370 277 246 191 123 15
BsrGI T'GTAC_A
Cuts at: 0 1739 1845
Size: 1739 106
BssHII G'CGCG_C
Cuts at: 0 726 1845
Size: 726 1119
BssSI C'ACGA_G
Cuts at: 0 883 1845
Size: 883 962
BstAPI GCAn_nnn'nTGC
Cuts at: 0 405 435 576 888 1110 1194 1805 1845
Size: 405 30 141 312 222 84 611 40
Fragments arranged by size:
611 405 312 222 141 84 40 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 483 1845
Size: 483 1362
BstYI r'GATC_y
Cuts at: 0 1406 1679 1845
Size: 1406 273 166
Fragments arranged by size:
1406 273 166
Bsu36I CC'TnA_GG
Cuts at: 0 1371 1845
Size: 1371 474
BtgI C'CryG_G
Cuts at: 0 333 440 1250 1286 1448 1505 1845
Size: 333 107 810 36 162 57 340
Fragments arranged by size:
810 340 333 162 107 57 36
BtsI GCAGTG_nn'
Cuts at: 0 1142 1434 1845
Size: 1142 292 411
Fragments arranged by size:
1142 411 292
DrdI GACnn_nn'nnGTC
Cuts at: 0 973 1845
Size: 973 872
EaeI y'GGCC_r
Cuts at: 0 86 158 416 473 504 512 686 721
Size: 86 72 258 57 31 8 174 35
Cuts at: 721 1067 1094 1257 1451 1521 1641 1845
Size: 346 27 163 194 70 120 204
Fragments arranged by size:
346 258 204 194 174 163 120 86
72 70 57 35 31 27 8
EagI C'GGCC_G
Cuts at: 0 86 416 686 721 1257 1521 1845
Size: 86 330 270 35 536 264 324
Fragments arranged by size:
536 330 324 270 264 86 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1845
Size: 19 1826
EcoICRI GAG'CTC
Cuts at: 0 1029 1845
Size: 1029 816
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 337 487 507 637 865 1357 1690
Size: 246 91 150 20 130 228 492 333
Cuts at: 1690 1845
Size: 155
Fragments arranged by size:
492 333 246 228 155 150 130 91
20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 148 1845
Size: 148 1697
EcoO109I rG'GnC_Cy
Cuts at: 0 64 101 102 153 1428 1770 1845
Size: 64 37 1 51 1275 342 75
Fragments arranged by size:
1275 342 75 64 51 37 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 132 1845
Size: 132 1713
FseI GG_CCGG'CC
Cuts at: 0 1643 1845
Size: 1643 202
HaeII r_GCGC'y
Cuts at: 0 47 75 371 624 1344 1477 1845
Size: 47 28 296 253 720 133 368
Fragments arranged by size:
720 368 296 253 133 47 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 877 879 909 910 911 942 1845
Size: 877 2 30 1 1 31 903
Fragments arranged by size:
903 877 31 30 2 1 1
KasI G'GCGC_C
Cuts at: 0 1473 1845
Size: 1473 372
MscI TGG'CCA
Cuts at: 0 160 514 1845
Size: 160 354 1331
Fragments arranged by size:
1331 354 160
MslI CAynn'nnrTG
Cuts at: 0 481 811 907 1036 1063 1845
Size: 481 330 96 129 27 782
Fragments arranged by size:
782 481 330 129 96 27
MspA1I CmG'CkG
Cuts at: 0 23 695 996 1124 1221 1565 1845
Size: 23 672 301 128 97 344 280
Fragments arranged by size:
672 344 301 280 128 97 23
NaeI GCC'GGC
Cuts at: 0 1272 1518 1641 1656 1845
Size: 1272 246 123 15 189
Fragments arranged by size:
1272 246 189 123 15
NarI GG'CG_CC
Cuts at: 0 1474 1845
Size: 1474 371
NcoI C'CATG_G
Cuts at: 0 1505 1845
Size: 1505 340
NgoMIV G'CCGG_C
Cuts at: 0 1270 1516 1639 1654 1845
Size: 1270 246 123 15 191
Fragments arranged by size:
1270 246 191 123 15
NotI GC'GGCC_GC
Cuts at: 0 721 1257 1521 1845
Size: 721 536 264 324
Fragments arranged by size:
721 536 324 264
NspI r_CATG'y
Cuts at: 0 288 1845
Size: 288 1557
PfoI T'CCnGG_A
Cuts at: 0 1781 1845
Size: 1781 64
PmlI CAC'GTG
Cuts at: 0 738 1061 1557 1845
Size: 738 323 496 288
Fragments arranged by size:
738 496 323 288
PpuMI rG'GwC_Cy
Cuts at: 0 1770 1845
Size: 1770 75
PspOMI G'GGCC_C
Cuts at: 0 57 63 101 1845
Size: 57 6 38 1744
Fragments arranged by size:
1744 57 38 6
PstI C_TGCA'G
Cuts at: 0 152 253 581 641 656 704 1054 1115
Size: 152 101 328 60 15 48 350 61
Cuts at: 1115 1190 1845
Size: 75 655
Fragments arranged by size:
655 350 328 152 101 75 61 60
48 15
PvuII CAG'CTG
Cuts at: 0 996 1221 1845
Size: 996 225 624
Fragments arranged by size:
996 624 225
SacI G_AGCT'C
Cuts at: 0 1031 1845
Size: 1031 814
SbfI CC_TGCA'GG
Cuts at: 0 152 1190 1845
Size: 152 1038 655
Fragments arranged by size:
1038 655 152
ScaI AGT'ACT
Cuts at: 0 1591 1845
Size: 1591 254
SexAI A'CCwGG_T
Cuts at: 0 860 1845
Size: 860 985
SfcI C'TryA_G
Cuts at: 0 148 249 363 577 637 652 700 1050
Size: 148 101 114 214 60 15 48 350
Cuts at: 1050 1111 1186 1845
Size: 61 75 659
Fragments arranged by size:
659 350 214 148 114 101 75 61
60 48 15
SfoI GGC'GCC
Cuts at: 0 1475 1845
Size: 1475 370
SgrAI Cr'CCGG_yG
Cuts at: 0 277 1845
Size: 277 1568
SmaI CCC'GGG
Cuts at: 0 62 1828 1845
Size: 62 1766 17
Fragments arranged by size:
1766 62 17
SmlI C'TyrA_G
Cuts at: 0 106 1845
Size: 106 1739
SphI G_CATG'C
Cuts at: 0 288 1845
Size: 288 1557
SrfI GCCC'GGGC
Cuts at: 0 62 1845
Size: 62 1783
StuI AGG'CCT
Cuts at: 0 217 850 1845
Size: 217 633 995
Fragments arranged by size:
995 633 217
StyI C'CwwG_G
Cuts at: 0 1505 1845
Size: 1505 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 141 963 1501 1845
Size: 141 822 538 344
Fragments arranged by size:
822 538 344 141
TatI w'GTAC_w
Cuts at: 0 1589 1739 1845
Size: 1589 150 106
Fragments arranged by size:
1589 150 106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 811 843 1845
Size: 811 32 1002
Fragments arranged by size:
1002 811 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 525 1845
Size: 525 1320
XmaI C'CCGG_G
Cuts at: 0 60 1826 1845
Size: 60 1766 19
Fragments arranged by size:
1766 60 19
ZraI GAC'GTC
Cuts at: 0 306 1845
Size: 306 1539
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI AvaI BanI BanII BbeI BbsI BglI
BglII Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI
EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI
HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PfoI PmlI PpuMI PspOMI
PstI PvuII SacI SbfI ScaI SexAI SfcI SfoI
SgrAI SmaI SmlI SphI SrfI StuI StyI TaqII
TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AloI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BlpI BmrI BmtI BplI BsaWI
BsaXI BsiWI BsmI BspEI BspHI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DraIII EarI EcoRI EcoRV
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NdeI NheI NruI NsiI PacI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SfiI SgfI SnaBI
SpeI SspI SwaI Tth111I XbaI XhoI XmnI