Restriction map for
pFN21AE4162
(Linear) (Six-base) MAPSORT of: KIEE4162.seq Check: 1194 from: 1 to: 1821
KIEE4162.orf
With 182 enzymes: SgfI *
March 22, 2012 17:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 225 1821
Size: 225 1596
AccI GT'mk_AC
Cuts at: 0 1160 1821
Size: 1160 661
Acc65I G'GTAC_C
Cuts at: 0 90 1433 1821
Size: 90 1343 388
Fragments arranged by size:
1343 388 90
AclI AA'CG_TT
Cuts at: 0 1469 1821
Size: 1469 352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 489 703 1399 1821
Size: 307 182 214 696 422
Fragments arranged by size:
696 422 307 214 182
AflIII A'CryG_T
Cuts at: 0 11 1821
Size: 11 1810
AhdI GACnn_n'nnGTC
Cuts at: 0 111 1821
Size: 111 1710
AleI CACnn'nnGTG
Cuts at: 0 359 1821
Size: 359 1462
AlwNI CAG_nnn'CTG
Cuts at: 0 357 409 1821
Size: 357 52 1412
Fragments arranged by size:
1412 357 52
ApaI G_GGCC'C
Cuts at: 0 232 1821
Size: 232 1589
ApoI r'AATT_y
Cuts at: 0 1343 1821
Size: 1343 478
AvaI C'yCGr_G
Cuts at: 0 145 163 1144 1821
Size: 145 18 981 677
Fragments arranged by size:
981 677 145 18
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 558 591 1821
Size: 558 33 1230
Fragments arranged by size:
1230 558 33
BanI G'GyrC_C
Cuts at: 0 90 695 1433 1821
Size: 90 605 738 388
Fragments arranged by size:
738 605 388 90
BanII G_rGCy'C
Cuts at: 0 232 923 1353 1786 1821
Size: 232 691 430 433 35
Fragments arranged by size:
691 433 430 232 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 1547 1821
Size: 1547 274
BbvCI CC'TCA_GC
Cuts at: 0 715 758 1821
Size: 715 43 1063
Fragments arranged by size:
1063 715 43
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 16 50 1569 1603 1821
Size: 16 34 1519 34 218
Fragments arranged by size:
1519 218 34 34 16
BciVI GTATCCnnnnn_n'
Cuts at: 0 559 1508 1821
Size: 559 949 313
Fragments arranged by size:
949 559 313
BclI T'GATC_A
Cuts at: 0 384 1821
Size: 384 1437
BlpI GC'TnA_GC
Cuts at: 0 1112 1821
Size: 1112 709
Bme1580I G_kGCm'C
Cuts at: 0 216 232 698 1821
Size: 216 16 466 1123
Fragments arranged by size:
1123 466 216 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 43 1626 1821
Size: 43 1583 195
Fragments arranged by size:
1583 195 43
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1708 1821
Size: 1708 113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 280 337 649 661 1207 1216 1283
Size: 76 204 57 312 12 546 9 67
Cuts at: 1283 1337 1821
Size: 54 484
Fragments arranged by size:
546 484 312 204 76 67 57 54
12 9
Bpu10I CC'TnA_GC
Cuts at: 0 715 758 1821
Size: 715 43 1063
Fragments arranged by size:
1063 715 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1678 1821
Size: 1678 143
BsaI GGTCTCn'nnnn_
Cuts at: 0 1168 1536 1821
Size: 1168 368 285
Fragments arranged by size:
1168 368 285
BsaBI GATnn'nnATC
Cuts at: 0 1235 1821
Size: 1235 586
BsaHI Gr'CG_yC
Cuts at: 0 29 1821
Size: 29 1792
BsaWI w'CCGG_w
Cuts at: 0 42 1550 1821
Size: 42 1508 271
Fragments arranged by size:
1508 271 42
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 357 664 742 889 955 1697 1746 1821
Size: 357 307 78 147 66 742 49 75
Fragments arranged by size:
742 357 307 147 78 75 66 49
BseYI C'CCAG_C
Cuts at: 0 134 1396 1821
Size: 134 1262 425
Fragments arranged by size:
1262 425 134
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 1009 1821
Size: 561 448 812
Fragments arranged by size:
812 561 448
BsiEI CG_ry'CG
Cuts at: 0 204 297 1821
Size: 204 93 1524
Fragments arranged by size:
1524 204 93
BsiHKAI G_wGCw'C
Cuts at: 0 746 923 1353 1611 1703 1821
Size: 746 177 430 258 92 118
Fragments arranged by size:
746 430 258 177 118 92
BsmBI CGTCTCn'nnnn_
Cuts at: 0 676 1821
Size: 676 1145
Bsp1286I G_dGCh'C
Cuts at: 0 216 232 698 746 923 1353 1611 1703
Size: 216 16 466 48 177 430 258 92
Cuts at: 1703 1786 1821
Size: 83 35
Fragments arranged by size:
466 430 258 216 177 92 83 48
35 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 225 1821
Size: 225 1596
BsrBI CCG'CTC
Cuts at: 0 1754 1821
Size: 1754 67
BsrFI r'CCGG_y
Cuts at: 0 61 87 306 1275 1821
Size: 61 26 219 969 546
Fragments arranged by size:
969 546 219 61 26
BssSI C'ACGA_G
Cuts at: 0 1610 1821
Size: 1610 211
BstAPI GCAn_nnn'nTGC
Cuts at: 0 903 1821
Size: 903 918
BstEII G'GTnAC_C
Cuts at: 0 658 1278 1821
Size: 658 620 543
Fragments arranged by size:
658 620 543
BstXI CCAn_nnnn'nTGG
Cuts at: 0 272 655 1821
Size: 272 383 1166
Fragments arranged by size:
1166 383 272
BtgI C'CryG_G
Cuts at: 0 198 1821
Size: 198 1623
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 738 1821
Size: 738 1083
BtsI GCAGTG_nn'
Cuts at: 0 82 265 549 1106 1821
Size: 82 183 284 557 715
Fragments arranged by size:
715 557 284 183 82
EaeI y'GGCC_r
Cuts at: 0 1008 1821
Size: 1008 813
EarI CTCTTCn'nnn_
Cuts at: 0 508 1058 1418 1821
Size: 508 550 360 403
Fragments arranged by size:
550 508 403 360
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1720 1821
Size: 1720 101
EcoICRI GAG'CTC
Cuts at: 0 921 1351 1821
Size: 921 430 470
Fragments arranged by size:
921 470 430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 280 307 337 489 649 661 703
Size: 76 204 27 30 152 160 12 42
Cuts at: 703 1207 1216 1283 1337 1399 1821
Size: 504 9 67 54 62 422
Fragments arranged by size:
504 422 204 160 152 76 67 62
54 42 30 27 12 9
EcoO109I rG'GnC_Cy
Cuts at: 0 153 160 1821
Size: 153 7 1661
Fragments arranged by size:
1661 153 7
EcoRI G'AATT_C
Cuts at: 0 1343 1821
Size: 1343 478
FseI GG_CCGG'CC
Cuts at: 0 65 1821
Size: 65 1756
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 1057 1089 1821
Size: 97 32 928 32 732
Fragments arranged by size:
928 732 97 32 32
HincII GTy'rAC
Cuts at: 0 312 1140 1821
Size: 312 828 681
Fragments arranged by size:
828 681 312
KpnI G_GTAC'C
Cuts at: 0 94 1437 1821
Size: 94 1343 384
Fragments arranged by size:
1343 384 94
MfeI C'AATT_G
Cuts at: 0 901 1821
Size: 901 920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1514 1736 1821
Size: 1514 222 85
Fragments arranged by size:
1514 222 85
MscI TGG'CCA
Cuts at: 0 1010 1821
Size: 1010 811
MslI CAynn'nnrTG
Cuts at: 0 359 1634 1821
Size: 359 1275 187
Fragments arranged by size:
1275 359 187
MspA1I CmG'CkG
Cuts at: 0 96 200 655 681 954 1194 1789 1821
Size: 96 104 455 26 273 240 595 32
Fragments arranged by size:
595 455 273 240 104 96 32 26
NaeI GCC'GGC
Cuts at: 0 63 1821
Size: 63 1758
NgoMIV G'CCGG_C
Cuts at: 0 61 1821
Size: 61 1760
NspI r_CATG'y
Cuts at: 0 583 1821
Size: 583 1238
PasI CC'CwG_GG
Cuts at: 0 691 1821
Size: 691 1130
PflMI CCAn_nnn'nTGG
Cuts at: 0 357 1821
Size: 357 1464
PpuMI rG'GwC_Cy
Cuts at: 0 153 160 1821
Size: 153 7 1661
Fragments arranged by size:
1661 153 7
PshAI GACnn'nnGTC
Cuts at: 0 158 1821
Size: 158 1663
PspOMI G'GGCC_C
Cuts at: 0 228 1821
Size: 228 1593
PspXI vC'TCGA_Gb
Cuts at: 0 1144 1821
Size: 1144 677
PstI C_TGCA'G
Cuts at: 0 179 917 953 1073 1821
Size: 179 738 36 120 748
Fragments arranged by size:
748 738 179 120 36
PvuII CAG'CTG
Cuts at: 0 655 681 954 1194 1821
Size: 655 26 273 240 627
Fragments arranged by size:
655 627 273 240 26
SacI G_AGCT'C
Cuts at: 0 923 1353 1821
Size: 923 430 468
Fragments arranged by size:
923 468 430
SacII CC_GC'GG
Cuts at: 0 201 1821
Size: 201 1620
SapI GCTCTTCn'nnn_
Cuts at: 0 1058 1821
Size: 1058 763
SfcI C'TryA_G
Cuts at: 0 175 342 913 949 1069 1821
Size: 175 167 571 36 120 752
Fragments arranged by size:
752 571 175 167 120 36
SmaI CCC'GGG
Cuts at: 0 147 1821
Size: 147 1674
SmlI C'TyrA_G
Cuts at: 0 1144 1693 1821
Size: 1144 549 128
Fragments arranged by size:
1144 549 128
StuI AGG'CCT
Cuts at: 0 1747 1821
Size: 1747 74
StyI C'CwwG_G
Cuts at: 0 476 1821
Size: 476 1345
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 349 989 1821
Size: 349 640 832
Fragments arranged by size:
832 640 349
TatI w'GTAC_w
Cuts at: 0 1418 1821
Size: 1418 403
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1482 1514 1693 1725 1821
Size: 1482 32 179 32 96
Fragments arranged by size:
1482 179 96 32 32
XbaI T'CTAG_A
Cuts at: 0 999 1821
Size: 999 822
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 127 849 1821
Size: 127 722 972
Fragments arranged by size:
972 722 127
XhoI C'TCGA_G
Cuts at: 0 1144 1821
Size: 1144 677
XmaI C'CCGG_G
Cuts at: 0 145 1821
Size: 145 1676
XmnI GAAnn'nnTTC
Cuts at: 0 13 1343 1471 1821
Size: 13 1330 128 350
Fragments arranged by size:
1330 350 128 13
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlwNI ApaI ApoI AvaI BaeI BanI BanII BbsI
BbvCI BcgI BciVI BclI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssSI BstAPI BstEII BstXI BtgI BtgZI BtsI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI
Hin4I HincII KpnI MfeI MmeI MscI MslI MspA1I
NaeI NgoMIV NspI PasI PflMI PpuMI PshAI PspOMI
PspXI PstI PvuII SacI SacII SapI SfcI SmaI
SmlI StuI StyI TaqII TatI TstI XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AlfI AloI ApaLI AscI
AseI AsiSI AvrII BamHI BbeI BfrBI BglI BglII
BmgBI BmtI BsaAI BsaXI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BssHII BstBI BstYI BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EagI EcoNI EcoRV FalI
FspI FspAI HaeII HindIII HpaI KasI MluI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PsiI PsrI PvuI RsrII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
ZraI