Restriction map for
pFN21AE4161
(Linear) (Six-base) MAPSORT of: KIEE4161.seq Check: 1755 from: 1 to: 1809
KIEE4161.orf
With 182 enzymes: SgfI *
July 3, 2012 18:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1504 1809
Size: 1504 305
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1437 1809
Size: 1437 372
AflIII A'CryG_T
Cuts at: 0 260 913 1352 1622 1809
Size: 260 653 439 270 187
Fragments arranged by size:
653 439 270 260 187
AleI CACnn'nnGTG
Cuts at: 0 323 1809
Size: 323 1486
AlwNI CAG_nnn'CTG
Cuts at: 0 229 232 592 766 886 1395 1809
Size: 229 3 360 174 120 509 414
Fragments arranged by size:
509 414 360 229 174 120 3
ApaI G_GGCC'C
Cuts at: 0 457 478 894 1256 1809
Size: 457 21 416 362 553
Fragments arranged by size:
553 457 416 362 21
ApaLI G'TGCA_C
Cuts at: 0 815 1491 1809
Size: 815 676 318
Fragments arranged by size:
815 676 318
AvaI C'yCGr_G
Cuts at: 0 280 754 893 1067 1429 1809
Size: 280 474 139 174 362 380
Fragments arranged by size:
474 380 362 280 174 139
BanI G'GyrC_C
Cuts at: 0 35 52 1017 1047 1150 1640 1809
Size: 35 17 965 30 103 490 169
Fragments arranged by size:
965 490 169 103 35 30 17
BanII G_rGCy'C
Cuts at: 0 457 478 812 894 1256 1809
Size: 457 21 334 82 362 553
Fragments arranged by size:
553 457 362 334 82 21
BbeI G_GCGC'C
Cuts at: 0 1051 1809
Size: 1051 758
BbvCI CC'TCA_GC
Cuts at: 0 467 483 1809
Size: 467 16 1326
Fragments arranged by size:
1326 467 16
BglI GCCn_nnn'nGGC
Cuts at: 0 452 1251 1733 1790 1809
Size: 452 799 482 57 19
Fragments arranged by size:
799 482 452 57 19
BglII A'GATC_T
Cuts at: 0 1448 1809
Size: 1448 361
Bme1580I G_kGCm'C
Cuts at: 0 40 368 457 478 819 894 1256 1318
Size: 40 328 89 21 341 75 362 62
Cuts at: 1318 1495 1809
Size: 177 314
Fragments arranged by size:
362 341 328 314 177 89 75 62
40 21
BmgBI CAC'GTC
Cuts at: 0 498 1101 1623 1809
Size: 498 603 522 186
Fragments arranged by size:
603 522 498 186
BmrI ACTGGGnnnn_n'
Cuts at: 0 378 830 1761 1809
Size: 378 452 931 48
Fragments arranged by size:
931 452 378 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1585 1809
Size: 1585 224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1216 1420 1575 1809
Size: 1216 204 155 234
Fragments arranged by size:
1216 234 204 155
Bpu10I CC'TnA_GC
Cuts at: 0 113 467 483 1809
Size: 113 354 16 1326
Fragments arranged by size:
1326 354 113 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 1471 1809
Size: 79 1392 338
Fragments arranged by size:
1392 338 79
BsaI GGTCTCn'nnnn_
Cuts at: 0 1556 1809
Size: 1556 253
BsaAI yAC'GTr
Cuts at: 0 263 1095 1353 1437 1809
Size: 263 832 258 84 372
Fragments arranged by size:
832 372 263 258 84
BsaHI Gr'CG_yC
Cuts at: 0 1048 1809
Size: 1048 761
BsaWI w'CCGG_w
Cuts at: 0 655 1809
Size: 655 1154
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 393 411 423 441 1162 1192 1809
Size: 393 18 12 18 721 30 617
Fragments arranged by size:
721 617 393 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 419 484 1183 1809
Size: 205 214 65 699 626
Fragments arranged by size:
699 626 214 205 65
BseYI C'CCAG_C
Cuts at: 0 200 585 958 1809
Size: 200 385 373 851
Fragments arranged by size:
851 385 373 200
BsiEI CG_ry'CG
Cuts at: 0 577 804 1274 1710 1794 1809
Size: 577 227 470 436 84 15
Fragments arranged by size:
577 470 436 227 84 15
BsiHKAI G_wGCw'C
Cuts at: 0 819 1436 1495 1809
Size: 819 617 59 314
Fragments arranged by size:
819 617 314 59
Bsp1286I G_dGCh'C
Cuts at: 0 40 368 457 478 812 819 894 1256
Size: 40 328 89 21 334 7 75 362
Cuts at: 1256 1318 1436 1495 1809
Size: 62 118 59 314
Fragments arranged by size:
362 334 328 314 118 89 75 62
59 40 21 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 512 1504 1673 1809
Size: 512 992 169 136
Fragments arranged by size:
992 512 169 136
BsrBI CCG'CTC
Cuts at: 0 213 750 1569 1809
Size: 213 537 819 240
Fragments arranged by size:
819 537 240 213
BsrDI GCAATG_nn'
Cuts at: 0 1773 1809
Size: 1773 36
BsrFI r'CCGG_y
Cuts at: 0 204 487 624 1346 1789 1809
Size: 204 283 137 722 443 20
Fragments arranged by size:
722 443 283 204 137 20
BssHII G'CGCG_C
Cuts at: 0 1470 1472 1809
Size: 1470 2 337
Fragments arranged by size:
1470 337 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 229 1595 1809
Size: 229 1366 214
Fragments arranged by size:
1366 229 214
BstXI CCAn_nnnn'nTGG
Cuts at: 0 325 328 636 966 1809
Size: 325 3 308 330 843
Fragments arranged by size:
843 330 325 308 3
BstYI r'GATC_y
Cuts at: 0 640 1373 1448 1809
Size: 640 733 75 361
Fragments arranged by size:
733 640 361 75
Bsu36I CC'TnA_GG
Cuts at: 0 1031 1809
Size: 1031 778
BtgI C'CryG_G
Cuts at: 0 6 141 689 1239 1809
Size: 6 135 548 550 570
Fragments arranged by size:
570 550 548 135 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 765 1592 1809
Size: 765 827 217
Fragments arranged by size:
827 765 217
BtsI GCAGTG_nn'
Cuts at: 0 1286 1809
Size: 1286 523
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 158 193 1809
Size: 158 35 1616
Fragments arranged by size:
1616 158 35
EaeI y'GGCC_r
Cuts at: 0 179 515 626 801 980 1271 1283 1478
Size: 179 336 111 175 179 291 12 195
Cuts at: 1478 1791 1809
Size: 313 18
Fragments arranged by size:
336 313 291 195 179 179 175 111
18 12
EagI C'GGCC_G
Cuts at: 0 801 1271 1791 1809
Size: 801 470 520 18
Fragments arranged by size:
801 520 470 18
EarI CTCTTCn'nnn_
Cuts at: 0 740 1228 1809
Size: 740 488 581
Fragments arranged by size:
740 581 488
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 414 597 1003 1046 1336 1809
Size: 414 183 406 43 290 473
Fragments arranged by size:
473 414 406 290 183 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1216 1420 1437 1575 1809
Size: 1216 204 17 138 234
Fragments arranged by size:
1216 234 204 138 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 437 739 1809
Size: 437 302 1070
Fragments arranged by size:
1070 437 302
EcoO109I rG'GnC_Cy
Cuts at: 0 189 453 454 474 475 732 763 883
Size: 189 264 1 20 1 257 31 120
Cuts at: 883 890 1253 1809
Size: 7 363 556
Fragments arranged by size:
556 363 264 257 189 120 31 20
7 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 321 1809
Size: 321 1488
FseI GG_CCGG'CC
Cuts at: 0 1793 1809
Size: 1793 16
FspI TGC'GCA
Cuts at: 0 778 1809
Size: 778 1031
FspAI rTGC'GCAy
Cuts at: 0 778 1809
Size: 778 1031
HaeII r_GCGC'y
Cuts at: 0 408 592 1051 1809
Size: 408 184 459 758
Fragments arranged by size:
758 459 408 184
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 393 425 493 525 1809
Size: 393 32 68 32 1284
Fragments arranged by size:
1284 393 68 32 32
KasI G'GCGC_C
Cuts at: 0 1047 1809
Size: 1047 762
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 821 1809
Size: 821 988
MscI TGG'CCA
Cuts at: 0 181 982 1809
Size: 181 801 827
Fragments arranged by size:
827 801 181
MslI CAynn'nnrTG
Cuts at: 0 323 1809
Size: 323 1486
MspA1I CmG'CkG
Cuts at: 0 229 543 827 1241 1392 1637 1809
Size: 229 314 284 414 151 245 172
Fragments arranged by size:
414 314 284 245 229 172 151
NaeI GCC'GGC
Cuts at: 0 206 1791 1809
Size: 206 1585 18
Fragments arranged by size:
1585 206 18
NarI GG'CG_CC
Cuts at: 0 1048 1809
Size: 1048 761
NcoI C'CATG_G
Cuts at: 0 141 1809
Size: 141 1668
NgoMIV G'CCGG_C
Cuts at: 0 204 1789 1809
Size: 204 1585 20
Fragments arranged by size:
1585 204 20
NspI r_CATG'y
Cuts at: 0 917 1419 1809
Size: 917 502 390
Fragments arranged by size:
917 502 390
PasI CC'CwG_GG
Cuts at: 0 439 766 1809
Size: 439 327 1043
Fragments arranged by size:
1043 439 327
PciI A'CATG_T
Cuts at: 0 913 1809
Size: 913 896
PflMI CCAn_nnn'nTGG
Cuts at: 0 142 766 886 1809
Size: 142 624 120 923
Fragments arranged by size:
923 624 142 120
PfoI T'CCnGG_A
Cuts at: 0 254 1809
Size: 254 1555
PmlI CAC'GTG
Cuts at: 0 263 1095 1353 1437 1809
Size: 263 832 258 84 372
Fragments arranged by size:
832 372 263 258 84
PpuMI rG'GwC_Cy
Cuts at: 0 189 883 1809
Size: 189 694 926
Fragments arranged by size:
926 694 189
PspOMI G'GGCC_C
Cuts at: 0 453 474 890 1252 1809
Size: 453 21 416 362 557
Fragments arranged by size:
557 453 416 362 21
PstI C_TGCA'G
Cuts at: 0 563 673 1809
Size: 563 110 1136
Fragments arranged by size:
1136 563 110
PvuII CAG'CTG
Cuts at: 0 229 1809
Size: 229 1580
RsrII CG'GwC_CG
Cuts at: 0 653 1809
Size: 653 1156
SacII CC_GC'GG
Cuts at: 0 1242 1809
Size: 1242 567
SapI GCTCTTCn'nnn_
Cuts at: 0 740 1228 1809
Size: 740 488 581
Fragments arranged by size:
740 581 488
SfcI C'TryA_G
Cuts at: 0 559 593 669 1809
Size: 559 34 76 1140
Fragments arranged by size:
1140 559 76 34
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 452 1251 1790 1809
Size: 452 799 539 19
Fragments arranged by size:
799 539 452 19
SfoI GGC'GCC
Cuts at: 0 1049 1809
Size: 1049 760
SmaI CCC'GGG
Cuts at: 0 282 756 1069 1809
Size: 282 474 313 740
Fragments arranged by size:
740 474 313 282
SmlI C'TyrA_G
Cuts at: 0 94 1486 1809
Size: 94 1392 323
Fragments arranged by size:
1392 323 94
SrfI GCCC'GGGC
Cuts at: 0 756 1809
Size: 756 1053
StuI AGG'CCT
Cuts at: 0 855 1029 1809
Size: 855 174 780
Fragments arranged by size:
855 780 174
StyI C'CwwG_G
Cuts at: 0 141 176 386 422 629 728 899 1809
Size: 141 35 210 36 207 99 171 910
Fragments arranged by size:
910 210 207 171 141 99 36 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 591 1743 1809
Size: 591 1152 66
Fragments arranged by size:
1152 591 66
TatI w'GTAC_w
Cuts at: 0 1412 1809
Size: 1412 397
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 411 443 1162 1194 1809
Size: 411 32 719 32 615
Fragments arranged by size:
719 615 411 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 325 1473 1809
Size: 325 1148 336
Fragments arranged by size:
1148 336 325
XmaI C'CCGG_G
Cuts at: 0 280 754 1067 1809
Size: 280 474 313 742
Fragments arranged by size:
742 474 313 280
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BbvCI BglI BglII Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssHII BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I FalI FseI FspI FspAI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmlI
PpuMI PspOMI PstI PvuII RsrII SacII SapI SfcI
SfiI SfoI SmaI SmlI SrfI StuI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BcgI BciVI BclI BfrBI BlpI BmtI
BsaBI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrGI
BssSI BstBI BstEII BstZ17I ClaI DraI DraIII DrdI
EcoICRI EcoRI EcoRV HincII HindIII HpaI KpnI MfeI
MluI NdeI NheI NotI NruI NsiI PacI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI SacI SalI
SanDI SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI
SphI SspI SwaI Tth111I XbaI XhoI XmnI ZraI