Restriction map for
pFN21AE4159
(Linear) (Six-base) MAPSORT of: KIEE4159.seq Check: 3736 from: 1 to: 1800
KIEE4159.orf
With 182 enzymes: SgfI *
July 3, 2012 18:40 ..
Acc65I G'GTAC_C
Cuts at: 0 869 1800
Size: 869 931
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 255 362 1325 1800
Size: 113 142 107 963 475
Fragments arranged by size:
963 475 142 113 107
AfeI AGC'GCT
Cuts at: 0 517 1800
Size: 517 1283
AflII C'TTAA_G
Cuts at: 0 1318 1800
Size: 1318 482
AflIII A'CryG_T
Cuts at: 0 759 1800
Size: 759 1041
ApoI r'AATT_y
Cuts at: 0 172 226 564 607 828 1048 1800
Size: 172 54 338 43 221 220 752
Fragments arranged by size:
752 338 221 220 172 54 43
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1475 1508 1800
Size: 1475 33 292
Fragments arranged by size:
1475 292 33
BanI G'GyrC_C
Cuts at: 0 869 1800
Size: 869 931
BanII G_rGCy'C
Cuts at: 0 752 1800
Size: 752 1048
BbsI GAAGACnn'nnnn_
Cuts at: 0 738 1659 1800
Size: 738 921 141
Fragments arranged by size:
921 738 141
BciVI GTATCCnnnnn_n'
Cuts at: 0 603 1503 1800
Size: 603 900 297
Fragments arranged by size:
900 603 297
BfrBI ATG'CAT
Cuts at: 0 460 1800
Size: 460 1340
BlpI GC'TnA_GC
Cuts at: 0 1037 1800
Size: 1037 763
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 265 788 1800
Size: 265 523 1012
Fragments arranged by size:
1012 523 265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 1800
Size: 142 1658
Bpu10I CC'TnA_GC
Cuts at: 0 504 1800
Size: 504 1296
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 1800
Size: 634 1166
BsaAI yAC'GTr
Cuts at: 0 1578 1800
Size: 1578 222
BsaHI Gr'CG_yC
Cuts at: 0 1194 1800
Size: 1194 606
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 734 764 1800
Size: 734 30 1036
Fragments arranged by size:
1036 734 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 493 691 1800
Size: 493 198 1109
Fragments arranged by size:
1109 493 198
BseYI C'CCAG_C
Cuts at: 0 57 596 1800
Size: 57 539 1204
Fragments arranged by size:
1204 539 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1475 1800
Size: 1475 325
BsiHKAI G_wGCw'C
Cuts at: 0 251 752 1080 1800
Size: 251 501 328 720
Fragments arranged by size:
720 501 328 251
Bsp1286I G_dGCh'C
Cuts at: 0 251 752 1080 1800
Size: 251 501 328 720
Fragments arranged by size:
720 501 328 251
BspHI T'CATG_A
Cuts at: 0 108 1800
Size: 108 1692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 908 1800
Size: 908 892
BsrDI GCAATG_nn'
Cuts at: 0 1736 1800
Size: 1736 64
BsrFI r'CCGG_y
Cuts at: 0 1519 1800
Size: 1519 281
BsrGI T'GTAC_A
Cuts at: 0 405 1800
Size: 405 1395
BssSI C'ACGA_G
Cuts at: 0 745 1800
Size: 745 1055
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1075 1800
Size: 1075 725
BstXI CCAn_nnnn'nTGG
Cuts at: 0 474 880 1800
Size: 474 406 920
Fragments arranged by size:
920 474 406
BstYI r'GATC_y
Cuts at: 0 36 1800
Size: 36 1764
BtsI GCAGTG_nn'
Cuts at: 0 1349 1800
Size: 1349 451
ClaI AT'CG_AT
Cuts at: 0 1296 1800
Size: 1296 504
DraI TTT'AAA
Cuts at: 0 1794 1800
Size: 1794 6
DraIII CAC_nnn'GTG
Cuts at: 0 998 1800
Size: 998 802
EarI CTCTTCn'nnn_
Cuts at: 0 132 553 1800
Size: 132 421 1247
Fragments arranged by size:
1247 421 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1664 1800
Size: 1664 136
EcoICRI GAG'CTC
Cuts at: 0 750 1800
Size: 750 1050
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 142 255 362 1325 1800
Size: 113 29 113 107 963 475
Fragments arranged by size:
963 475 113 113 107 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1013 1800
Size: 1013 787
EcoO109I rG'GnC_Cy
Cuts at: 0 1384 1800
Size: 1384 416
EcoRI G'AATT_C
Cuts at: 0 828 1800
Size: 828 972
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 628 649 1800
Size: 628 21 1151
Fragments arranged by size:
1151 628 21
HaeII r_GCGC'y
Cuts at: 0 519 1800
Size: 519 1281
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 79 111 577 609 1800
Size: 79 32 466 32 1191
Fragments arranged by size:
1191 466 79 32 32
HindIII A'AGCT_T
Cuts at: 0 1039 1800
Size: 1039 761
KpnI G_GTAC'C
Cuts at: 0 873 1800
Size: 873 927
MfeI C'AATT_G
Cuts at: 0 817 1466 1800
Size: 817 649 334
Fragments arranged by size:
817 649 334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 933 951 1800
Size: 933 18 849
Fragments arranged by size:
933 849 18
MslI CAynn'nnrTG
Cuts at: 0 211 1757 1800
Size: 211 1546 43
Fragments arranged by size:
1546 211 43
MspA1I CmG'CkG
Cuts at: 0 1489 1800
Size: 1489 311
NsiI A_TGCA'T
Cuts at: 0 462 1800
Size: 462 1338
NspI r_CATG'y
Cuts at: 0 464 763 1800
Size: 464 299 1037
Fragments arranged by size:
1037 464 299
PciI A'CATG_T
Cuts at: 0 759 1800
Size: 759 1041
PflMI CCAn_nnn'nTGG
Cuts at: 0 1442 1800
Size: 1442 358
PpuMI rG'GwC_Cy
Cuts at: 0 1384 1800
Size: 1384 416
PsiI TTA'TAA
Cuts at: 0 1139 1800
Size: 1139 661
PstI C_TGCA'G
Cuts at: 0 1344 1800
Size: 1344 456
PvuII CAG'CTG
Cuts at: 0 1489 1800
Size: 1489 311
SacI G_AGCT'C
Cuts at: 0 752 1800
Size: 752 1048
SfcI C'TryA_G
Cuts at: 0 258 395 1340 1800
Size: 258 137 945 460
Fragments arranged by size:
945 460 258 137
SmlI C'TyrA_G
Cuts at: 0 613 1318 1800
Size: 613 705 482
Fragments arranged by size:
705 613 482
SspI AAT'ATT
Cuts at: 0 11 1155 1800
Size: 11 1144 645
Fragments arranged by size:
1144 645 11
StyI C'CwwG_G
Cuts at: 0 901 1215 1532 1735 1800
Size: 901 314 317 203 65
Fragments arranged by size:
901 317 314 203 65
TatI w'GTAC_w
Cuts at: 0 405 1800
Size: 405 1395
XmnI GAAnn'nnTTC
Cuts at: 0 608 1588 1800
Size: 608 980 212
Fragments arranged by size:
980 608 212
Enzymes that do cut:
Acc65I AcuI AfeI AflII AflIII ApoI BaeI BanI
BanII BbsI BciVI BfrBI BlpI BplI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI
BstXI BstYI BtsI ClaI DraI DraIII EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HindIII KpnI MfeI MmeI MslI MspA1I NsiI NspI
PciI PflMI PpuMI PsiI PstI PvuII SacI SfcI
SmlI SspI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AgeI AhdI AleI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BamHI BbeI BbvCI BcgI BclI BglI BglII
Bme1580I BmgBI BmrI BmtI BsaI BsaBI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BsrBI BssHII BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI CspCI DrdI EaeI EagI
EcoRV FseI FspI FspAI HincII HpaI KasI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PfoI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI ZraI