Restriction map for
pFN21AE4157
(Linear) (Six-base) MAPSORT of: KIEE4157.seq Check: 5442 from: 1 to: 1791
KIEE4157.orf
With 182 enzymes: SgfI *
March 22, 2012 17:29 ..
AatII G_ACGT'C
Cuts at: 0 491 1791
Size: 491 1300
Acc65I G'GTAC_C
Cuts at: 0 1612 1791
Size: 1612 179
AclI AA'CG_TT
Cuts at: 0 563 1791
Size: 563 1228
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 1738 1791
Size: 255 1483 53
Fragments arranged by size:
1483 255 53
AfeI AGC'GCT
Cuts at: 0 56 1791
Size: 56 1735
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1361 1791
Size: 1361 430
AlwNI CAG_nnn'CTG
Cuts at: 0 705 1791
Size: 705 1086
ApoI r'AATT_y
Cuts at: 0 505 1524 1791
Size: 505 1019 267
Fragments arranged by size:
1019 505 267
AvaI C'yCGr_G
Cuts at: 0 803 1791
Size: 803 988
BanI G'GyrC_C
Cuts at: 0 1612 1661 1791
Size: 1612 49 130
Fragments arranged by size:
1612 130 49
BbvCI CC'TCA_GC
Cuts at: 0 5 29 1791
Size: 5 24 1762
Fragments arranged by size:
1762 24 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 866 900 1791
Size: 866 34 891
Fragments arranged by size:
891 866 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 418 1791
Size: 418 1373
BfrBI ATG'CAT
Cuts at: 0 881 1532 1791
Size: 881 651 259
Fragments arranged by size:
881 651 259
BglII A'GATC_T
Cuts at: 0 1599 1791
Size: 1599 192
BlpI GC'TnA_GC
Cuts at: 0 58 757 1791
Size: 58 699 1034
Fragments arranged by size:
1034 699 58
Bme1580I G_kGCm'C
Cuts at: 0 889 1791
Size: 889 902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 666 1791
Size: 490 176 1125
Fragments arranged by size:
1125 490 176
Bpu10I CC'TnA_GC
Cuts at: 0 5 29 1026 1791
Size: 5 24 997 765
Fragments arranged by size:
997 765 24 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 1791
Size: 571 1220
BsaAI yAC'GTr
Cuts at: 0 1629 1791
Size: 1629 162
BsaHI Gr'CG_yC
Cuts at: 0 488 1442 1791
Size: 488 954 349
Fragments arranged by size:
954 488 349
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 130 1154 1383 1791
Size: 88 42 1024 229 408
Fragments arranged by size:
1024 408 229 88 42
BseYI C'CCAG_C
Cuts at: 0 1334 1791
Size: 1334 457
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 1730 1791
Size: 148 1582 61
Fragments arranged by size:
1582 148 61
BsmBI CGTCTCn'nnnn_
Cuts at: 0 479 1791
Size: 479 1312
Bsp1286I G_dGCh'C
Cuts at: 0 889 1791
Size: 889 902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 338 1791
Size: 338 1453
BsrBI CCG'CTC
Cuts at: 0 756 1381 1791
Size: 756 625 410
Fragments arranged by size:
756 625 410
BsrDI GCAATG_nn'
Cuts at: 0 383 576 1066 1721 1791
Size: 383 193 490 655 70
Fragments arranged by size:
655 490 383 193 70
BsrGI T'GTAC_A
Cuts at: 0 1127 1791
Size: 1127 664
BstYI r'GATC_y
Cuts at: 0 119 254 1505 1599 1791
Size: 119 135 1251 94 192
Fragments arranged by size:
1251 192 135 119 94
BtgI C'CryG_G
Cuts at: 0 954 1169 1223 1274 1791
Size: 954 215 54 51 517
Fragments arranged by size:
954 517 215 54 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 796 886 891 1791
Size: 796 90 5 900
Fragments arranged by size:
900 796 90 5
BtsI GCAGTG_nn'
Cuts at: 0 18 1345 1791
Size: 18 1327 446
Fragments arranged by size:
1327 446 18
DraI TTT'AAA
Cuts at: 0 1497 1641 1791
Size: 1497 144 150
Fragments arranged by size:
1497 150 144
EaeI y'GGCC_r
Cuts at: 0 149 788 1791
Size: 149 639 1003
Fragments arranged by size:
1003 639 149
EarI CTCTTCn'nnn_
Cuts at: 0 836 1791
Size: 836 955
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1732 1791
Size: 1732 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 490 666 1738 1791
Size: 255 235 176 1072 53
Fragments arranged by size:
1072 255 235 176 53
EcoO109I rG'GnC_Cy
Cuts at: 0 1331 1791
Size: 1331 460
EcoRI G'AATT_C
Cuts at: 0 505 1791
Size: 505 1286
EcoRV GAT'ATC
Cuts at: 0 233 1791
Size: 233 1558
HaeII r_GCGC'y
Cuts at: 0 58 1791
Size: 58 1733
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 637 669 716 748 1791
Size: 187 32 418 32 47 32 1043
Fragments arranged by size:
1043 418 187 47 32 32 32
HindIII A'AGCT_T
Cuts at: 0 529 1297 1735 1791
Size: 529 768 438 56
Fragments arranged by size:
768 529 438 56
KpnI G_GTAC'C
Cuts at: 0 1616 1791
Size: 1616 175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 1712 1791
Size: 656 1056 79
Fragments arranged by size:
1056 656 79
MscI TGG'CCA
Cuts at: 0 790 1791
Size: 790 1001
MspA1I CmG'CkG
Cuts at: 0 325 1791
Size: 325 1466
NcoI C'CATG_G
Cuts at: 0 954 1169 1223 1274 1791
Size: 954 215 54 51 517
Fragments arranged by size:
954 517 215 54 51
NdeI CA'TA_TG
Cuts at: 0 1351 1791
Size: 1351 440
NsiI A_TGCA'T
Cuts at: 0 883 1534 1791
Size: 883 651 257
Fragments arranged by size:
883 651 257
NspI r_CATG'y
Cuts at: 0 885 942 1791
Size: 885 57 849
Fragments arranged by size:
885 849 57
PasI CC'CwG_GG
Cuts at: 0 1446 1791
Size: 1446 345
PfoI T'CCnGG_A
Cuts at: 0 257 1791
Size: 257 1534
PpuMI rG'GwC_Cy
Cuts at: 0 1331 1791
Size: 1331 460
PshAI GACnn'nnGTC
Cuts at: 0 1225 1791
Size: 1225 566
PsiI TTA'TAA
Cuts at: 0 212 1791
Size: 212 1579
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 704 736 1791
Size: 704 32 1055
Fragments arranged by size:
1055 704 32
PstI C_TGCA'G
Cuts at: 0 1751 1791
Size: 1751 40
PvuII CAG'CTG
Cuts at: 0 325 1791
Size: 325 1466
SexAI A'CCwGG_T
Cuts at: 0 98 1791
Size: 98 1693
SfcI C'TryA_G
Cuts at: 0 1310 1571 1747 1791
Size: 1310 261 176 44
Fragments arranged by size:
1310 261 176 44
SmaI CCC'GGG
Cuts at: 0 805 1791
Size: 805 986
SmlI C'TyrA_G
Cuts at: 0 550 1791
Size: 550 1241
SphI G_CATG'C
Cuts at: 0 942 1791
Size: 942 849
StyI C'CwwG_G
Cuts at: 0 954 1169 1223 1274 1791
Size: 954 215 54 51 517
Fragments arranged by size:
954 517 215 54 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1267 1791
Size: 1267 524
TatI w'GTAC_w
Cuts at: 0 1127 1791
Size: 1127 664
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 465 1791
Size: 465 1326
XmaI C'CCGG_G
Cuts at: 0 803 1791
Size: 803 988
ZraI GAC'GTC
Cuts at: 0 489 1791
Size: 489 1302
Enzymes that do cut:
AatII Acc65I AclI AcuI AfeI AlfI AlwNI ApoI
AvaI BanI BbvCI BcgI BciVI BfrBI BglII BlpI
Bme1580I BpmI Bpu10I BpuEI BsaAI BsaHI BseRI BseYI
BsgI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrGI BstYI
BtgI BtgZI BtsI DraI EaeI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV HaeII Hin4I HindIII KpnI MmeI
MscI MspA1I NcoI NdeI NsiI NspI PasI PfoI
PpuMI PshAI PsiI PsrI PstI PvuII SexAI SfcI
SmaI SmlI SphI StyI TaqII TatI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AccI AflII AflIII AgeI AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanII BbeI BbsI BclI BglI BmgBI BmrI BmtI
BplI BsaI BsaBI BsaWI BsaXI BsiEI BsiHKAI BsiWI
BsmI BspEI BspHI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII DrdI
EagI EcoICRI EcoNI FalI FseI FspI FspAI HincII
HpaI KasI MfeI MluI MslI NaeI NarI NgoMIV
NheI NotI NruI PacI PciI PflMI PmeI PmlI
PpiI PspOMI PspXI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmnI