Restriction map for
pFN21AE4153
(Linear) (Six-base) MAPSORT of: KIEE4153.seq Check: 5322 from: 1 to: 1764
KIEE4153.orf
With 182 enzymes: SgfI *
March 22, 2012 17:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1105 1764
Size: 1105 659
AccI GT'mk_AC
Cuts at: 0 481 1149 1764
Size: 481 668 615
Fragments arranged by size:
668 615 481
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1561 1764
Size: 1561 203
AflIII A'CryG_T
Cuts at: 0 671 1764
Size: 671 1093
AhdI GACnn_n'nnGTC
Cuts at: 0 477 1764
Size: 477 1287
AleI CACnn'nnGTG
Cuts at: 0 695 1764
Size: 695 1069
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 884 1764
Size: 884 880
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 671 703 1764
Size: 671 32 1061
Fragments arranged by size:
1061 671 32
ApoI r'AATT_y
Cuts at: 0 313 1365 1631 1764
Size: 313 1052 266 133
Fragments arranged by size:
1052 313 266 133
BanII G_rGCy'C
Cuts at: 0 176 466 1764
Size: 176 290 1298
Fragments arranged by size:
1298 290 176
BbsI GAAGACnn'nnnn_
Cuts at: 0 32 1057 1764
Size: 32 1025 707
Fragments arranged by size:
1025 707 32
BbvCI CC'TCA_GC
Cuts at: 0 1294 1764
Size: 1294 470
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1603 1637 1764
Size: 1603 34 127
Fragments arranged by size:
1603 127 34
BclI T'GATC_A
Cuts at: 0 488 1764
Size: 488 1276
BfrBI ATG'CAT
Cuts at: 0 292 543 1233 1347 1764
Size: 292 251 690 114 417
Fragments arranged by size:
690 417 292 251 114
BmgBI CAC'GTC
Cuts at: 0 672 1764
Size: 672 1092
BmrI ACTGGGnnnn_n'
Cuts at: 0 883 1250 1764
Size: 883 367 514
Fragments arranged by size:
883 514 367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 1636 1764
Size: 160 1476 128
Fragments arranged by size:
1476 160 128
Bpu10I CC'TnA_GC
Cuts at: 0 1294 1764
Size: 1294 470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 295 1764
Size: 190 105 1469
Fragments arranged by size:
1469 190 105
BsaI GGTCTCn'nnnn_
Cuts at: 0 427 1654 1764
Size: 427 1227 110
Fragments arranged by size:
1227 427 110
BsaBI GATnn'nnATC
Cuts at: 0 1413 1764
Size: 1413 351
BsaHI Gr'CG_yC
Cuts at: 0 644 730 1764
Size: 644 86 1034
Fragments arranged by size:
1034 644 86
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 590 620 1199 1229 1764
Size: 590 30 579 30 535
Fragments arranged by size:
590 579 535 30 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 1139 1532 1764
Size: 74 1065 393 232
Fragments arranged by size:
1065 393 232 74
BsiHKAI G_wGCw'C
Cuts at: 0 126 176 1764
Size: 126 50 1588
Fragments arranged by size:
1588 126 50
Bsp1286I G_dGCh'C
Cuts at: 0 126 176 466 1764
Size: 126 50 290 1298
Fragments arranged by size:
1298 290 126 50
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1105 1764
Size: 1105 659
BsrDI GCAATG_nn'
Cuts at: 0 209 235 545 609 1089 1764
Size: 209 26 310 64 480 675
Fragments arranged by size:
675 480 310 209 64 26
BssSI C'ACGA_G
Cuts at: 0 1493 1764
Size: 1493 271
BstBI TT'CG_AA
Cuts at: 0 1635 1764
Size: 1635 129
BstXI CCAn_nnnn'nTGG
Cuts at: 0 697 1764
Size: 697 1067
BstYI r'GATC_y
Cuts at: 0 1134 1764
Size: 1134 630
BtgI C'CryG_G
Cuts at: 0 1203 1764
Size: 1203 561
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 543 1764
Size: 543 1221
BtsI GCAGTG_nn'
Cuts at: 0 1166 1764
Size: 1166 598
DraI TTT'AAA
Cuts at: 0 1725 1764
Size: 1725 39
EaeI y'GGCC_r
Cuts at: 0 1570 1764
Size: 1570 194
EarI CTCTTCn'nnn_
Cuts at: 0 1388 1764
Size: 1388 376
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 733 1570 1764
Size: 733 837 194
Fragments arranged by size:
837 733 194
EcoICRI GAG'CTC
Cuts at: 0 174 1764
Size: 174 1590
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 1561 1636 1764
Size: 160 1401 75 128
Fragments arranged by size:
1401 160 128 75
EcoRI G'AATT_C
Cuts at: 0 313 1764
Size: 313 1451
EcoRV GAT'ATC
Cuts at: 0 573 1764
Size: 573 1191
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 49 1764
Size: 49 1715
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 463 495 590 614 622 646 715 747
Size: 463 32 95 24 8 24 69 32
Cuts at: 747 1199 1231 1764
Size: 452 32 533
Fragments arranged by size:
533 463 452 95 69 32 32 32
24 24 8
HincII GTy'rAC
Cuts at: 0 482 1002 1764
Size: 482 520 762
Fragments arranged by size:
762 520 482
HindIII A'AGCT_T
Cuts at: 0 1684 1764
Size: 1684 80
MfeI C'AATT_G
Cuts at: 0 1218 1764
Size: 1218 546
MscI TGG'CCA
Cuts at: 0 1572 1764
Size: 1572 192
MslI CAynn'nnrTG
Cuts at: 0 695 1238 1374 1764
Size: 695 543 136 390
Fragments arranged by size:
695 543 390 136
NcoI C'CATG_G
Cuts at: 0 1203 1764
Size: 1203 561
NsiI A_TGCA'T
Cuts at: 0 294 545 1235 1349 1764
Size: 294 251 690 114 415
Fragments arranged by size:
690 415 294 251 114
NspI r_CATG'y
Cuts at: 0 1237 1347 1764
Size: 1237 110 417
Fragments arranged by size:
1237 417 110
PfoI T'CCnGG_A
Cuts at: 0 176 1764
Size: 176 1588
PstI C_TGCA'G
Cuts at: 0 1116 1764
Size: 1116 648
SacI G_AGCT'C
Cuts at: 0 176 1764
Size: 176 1588
SalI G'TCGA_C
Cuts at: 0 480 1764
Size: 480 1284
SfcI C'TryA_G
Cuts at: 0 1112 1764
Size: 1112 652
SmlI C'TyrA_G
Cuts at: 0 169 274 1764
Size: 169 105 1490
Fragments arranged by size:
1490 169 105
SphI G_CATG'C
Cuts at: 0 1347 1764
Size: 1347 417
StyI C'CwwG_G
Cuts at: 0 1203 1462 1764
Size: 1203 259 302
Fragments arranged by size:
1203 302 259
TatI w'GTAC_w
Cuts at: 0 1497 1557 1764
Size: 1497 60 207
Fragments arranged by size:
1497 207 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1404 1436 1764
Size: 1404 32 328
Fragments arranged by size:
1404 328 32
XbaI T'CTAG_A
Cuts at: 0 969 1764
Size: 969 795
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 616 1764
Size: 616 1148
XmnI GAAnn'nnTTC
Cuts at: 0 1103 1764
Size: 1103 661
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AloI
ApoI BanII BbsI BbvCI BcgI BclI BfrBI BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI
BsgI BsiHKAI Bsp1286I BspMI BsrDI BssSI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI EaeI EarI EciI
EcoICRI Eco57MI EcoRI EcoRV FalI Hin4I HincII HindIII
MfeI MscI MslI NcoI NsiI NspI PfoI PstI
SacI SalI SfcI SmlI SphI StyI TatI TstI
XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AlwNI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BbeI BciVI BglI BglII BlpI Bme1580I BmtI
BplI BsaAI BsaWI BseRI BseYI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstAPI
BstEII BstZ17I Bsu36I ClaI CspCI DraIII DrdI EagI
EcoNI EcoO109I FseI FspI FspAI HaeII HpaI KasI
KpnI MluI MmeI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI TaqII Tth111I XhoI XmaI
ZraI