Restriction map for
pFN21AE4145
(Linear) (Six-base) MAPSORT of: KIEE4145.seq Check: 7476 from: 1 to: 1734
KIEE4145.orf
With 182 enzymes: SgfI *
March 22, 2012 17:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 41 650 841 1734
Size: 41 609 191 893
Fragments arranged by size:
893 609 191 41
AatII G_ACGT'C
Cuts at: 0 104 1734
Size: 104 1630
AccI GT'mk_AC
Cuts at: 0 1448 1734
Size: 1448 286
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1075 1111 1734
Size: 1075 36 623
Fragments arranged by size:
1075 623 36
AflIII A'CryG_T
Cuts at: 0 1451 1734
Size: 1451 283
AhdI GACnn_n'nnGTC
Cuts at: 0 1023 1734
Size: 1023 711
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 407 1734
Size: 407 1327
AlwNI CAG_nnn'CTG
Cuts at: 0 75 387 689 702 798 834 1292 1734
Size: 75 312 302 13 96 36 458 442
Fragments arranged by size:
458 442 312 302 96 75 36 13
ApaLI G'TGCA_C
Cuts at: 0 664 1734
Size: 664 1070
AvaI C'yCGr_G
Cuts at: 0 524 589 629 646 1001 1462 1734
Size: 524 65 40 17 355 461 272
Fragments arranged by size:
524 461 355 272 65 40 17
BanI G'GyrC_C
Cuts at: 0 774 1080 1150 1524 1639 1734
Size: 774 306 70 374 115 95
Fragments arranged by size:
774 374 306 115 95 70
BanII G_rGCy'C
Cuts at: 0 242 719 863 1285 1418 1734
Size: 242 477 144 422 133 316
Fragments arranged by size:
477 422 316 242 144 133
BbeI G_GCGC'C
Cuts at: 0 1084 1643 1734
Size: 1084 559 91
Fragments arranged by size:
1084 559 91
BbsI GAAGACnn'nnnn_
Cuts at: 0 1674 1734
Size: 1674 60
BglI GCCn_nnn'nGGC
Cuts at: 0 1117 1734
Size: 1117 617
BglII A'GATC_T
Cuts at: 0 1133 1734
Size: 1133 601
BlpI GC'TnA_GC
Cuts at: 0 157 655 1734
Size: 157 498 1079
Fragments arranged by size:
1079 498 157
Bme1580I G_kGCm'C
Cuts at: 0 668 683 777 1009 1734
Size: 668 15 94 232 725
Fragments arranged by size:
725 668 232 94 15
BmgBI CAC'GTC
Cuts at: 0 234 1164 1734
Size: 234 930 570
Fragments arranged by size:
930 570 234
BmrI ACTGGGnnnn_n'
Cuts at: 0 1540 1734
Size: 1540 194
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 225 1734
Size: 225 1509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 820 856 1269 1734
Size: 772 48 36 413 465
Fragments arranged by size:
772 465 413 48 36
BsaAI yAC'GTr
Cuts at: 0 499 1452 1548 1590 1734
Size: 499 953 96 42 144
Fragments arranged by size:
953 499 144 96 42
BsaHI Gr'CG_yC
Cuts at: 0 101 674 1081 1207 1640 1734
Size: 101 573 407 126 433 94
Fragments arranged by size:
573 433 407 126 101 94
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 770 800 1657 1687 1734
Size: 770 30 857 30 47
Fragments arranged by size:
857 770 47 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 817 853 856 859 871 1734
Size: 817 36 3 3 12 863
Fragments arranged by size:
863 817 36 12 3 3
BseYI C'CCAG_C
Cuts at: 0 441 605 1706 1734
Size: 441 164 1101 28
Fragments arranged by size:
1101 441 164 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 373 715 1385 1390 1507 1734
Size: 322 51 342 670 5 117 227
Fragments arranged by size:
670 342 322 227 117 51 5
BsiEI CG_ry'CG
Cuts at: 0 123 369 1560 1734
Size: 123 246 1191 174
Fragments arranged by size:
1191 246 174 123
BsiHKAI G_wGCw'C
Cuts at: 0 668 1418 1733 1734
Size: 668 750 315 1
Fragments arranged by size:
750 668 315 1
Bsp1286I G_dGCh'C
Cuts at: 0 242 668 683 719 777 863 1009 1285
Size: 242 426 15 36 58 86 146 276
Cuts at: 1285 1418 1733 1734
Size: 133 315 1
Fragments arranged by size:
426 315 276 242 146 133 86 58
36 15 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 41 383 555 566 650 841 1734
Size: 41 342 172 11 84 191 893
Fragments arranged by size:
893 342 191 172 84 41 11
BsrBI CCG'CTC
Cuts at: 0 531 1734
Size: 531 1203
BsrFI r'CCGG_y
Cuts at: 0 281 550 1252 1265 1734
Size: 281 269 702 13 469
Fragments arranged by size:
702 469 281 269 13
BsrGI T'GTAC_A
Cuts at: 0 272 1734
Size: 272 1462
BssHII G'CGCG_C
Cuts at: 0 214 331 1517 1734
Size: 214 117 1186 217
Fragments arranged by size:
1186 217 214 117
BstAPI GCAn_nnn'nTGC
Cuts at: 0 75 438 702 1485 1503 1734
Size: 75 363 264 783 18 231
Fragments arranged by size:
783 363 264 231 75 18
BstXI CCAn_nnnn'nTGG
Cuts at: 0 405 1734
Size: 405 1329
BstYI r'GATC_y
Cuts at: 0 1133 1734
Size: 1133 601
BtgI C'CryG_G
Cuts at: 0 245 1076 1635 1665 1734
Size: 245 831 559 30 69
Fragments arranged by size:
831 559 245 69 30
BtsI GCAGTG_nn'
Cuts at: 0 106 1324 1402 1734
Size: 106 1218 78 332
Fragments arranged by size:
1218 332 106 78
DraIII CAC_nnn'GTG
Cuts at: 0 1534 1734
Size: 1534 200
EaeI y'GGCC_r
Cuts at: 0 95 120 366 548 1557 1734
Size: 95 25 246 182 1009 177
Fragments arranged by size:
1009 246 182 177 95 25
EagI C'GGCC_G
Cuts at: 0 120 366 1557 1734
Size: 120 246 1191 177
Fragments arranged by size:
1191 246 177 120
EcoICRI GAG'CTC
Cuts at: 0 1416 1734
Size: 1416 318
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 820 856 1075 1111 1269 1734
Size: 772 48 36 219 36 158 465
Fragments arranged by size:
772 465 219 158 48 36 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 562 724 757 1057 1734
Size: 562 162 33 300 677
Fragments arranged by size:
677 562 300 162 33
EcoO109I rG'GnC_Cy
Cuts at: 0 594 686 807 1670 1734
Size: 594 92 121 863 64
Fragments arranged by size:
863 594 121 92 64
HaeII r_GCGC'y
Cuts at: 0 75 157 316 835 1084 1292 1643 1734
Size: 75 82 159 519 249 208 351 91
Fragments arranged by size:
519 351 249 208 159 91 82 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1009 1041 1734
Size: 1009 32 693
Fragments arranged by size:
1009 693 32
HincII GTy'rAC
Cuts at: 0 1028 1734
Size: 1028 706
KasI G'GCGC_C
Cuts at: 0 1080 1639 1734
Size: 1080 559 95
Fragments arranged by size:
1080 559 95
MspA1I CmG'CkG
Cuts at: 0 1202 1562 1734
Size: 1202 360 172
Fragments arranged by size:
1202 360 172
NaeI GCC'GGC
Cuts at: 0 283 552 1254 1267 1734
Size: 283 269 702 13 467
Fragments arranged by size:
702 467 283 269 13
NarI GG'CG_CC
Cuts at: 0 1081 1640 1734
Size: 1081 559 94
Fragments arranged by size:
1081 559 94
NgoMIV G'CCGG_C
Cuts at: 0 281 550 1252 1265 1734
Size: 281 269 702 13 469
Fragments arranged by size:
702 469 281 269 13
NotI GC'GGCC_GC
Cuts at: 0 366 1557 1734
Size: 366 1191 177
Fragments arranged by size:
1191 366 177
NspI r_CATG'y
Cuts at: 0 46 57 743 1734
Size: 46 11 686 991
Fragments arranged by size:
991 686 46 11
PasI CC'CwG_GG
Cuts at: 0 759 1734
Size: 759 975
PflMI CCAn_nnn'nTGG
Cuts at: 0 461 911 1475 1734
Size: 461 450 564 259
Fragments arranged by size:
564 461 450 259
PfoI T'CCnGG_A
Cuts at: 0 725 1066 1685 1734
Size: 725 341 619 49
Fragments arranged by size:
725 619 341 49
PmlI CAC'GTG
Cuts at: 0 499 1590 1734
Size: 499 1091 144
Fragments arranged by size:
1091 499 144
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 493 525 1734
Size: 493 32 1209
Fragments arranged by size:
1209 493 32
PpuMI rG'GwC_Cy
Cuts at: 0 807 1734
Size: 807 927
PshAI GACnn'nnGTC
Cuts at: 0 1210 1734
Size: 1210 524
PspXI vC'TCGA_Gb
Cuts at: 0 589 1734
Size: 589 1145
PstI C_TGCA'G
Cuts at: 0 71 380 518 566 1319 1734
Size: 71 309 138 48 753 415
Fragments arranged by size:
753 415 309 138 71 48
PvuII CAG'CTG
Cuts at: 0 1202 1734
Size: 1202 532
SacI G_AGCT'C
Cuts at: 0 1418 1734
Size: 1418 316
SexAI A'CCwGG_T
Cuts at: 0 1021 1274 1734
Size: 1021 253 460
Fragments arranged by size:
1021 460 253
SfcI C'TryA_G
Cuts at: 0 67 376 514 562 1315 1734
Size: 67 309 138 48 753 419
Fragments arranged by size:
753 419 309 138 67 48
SfoI GGC'GCC
Cuts at: 0 1082 1641 1734
Size: 1082 559 93
Fragments arranged by size:
1082 559 93
SmaI CCC'GGG
Cuts at: 0 631 1464 1734
Size: 631 833 270
Fragments arranged by size:
833 631 270
SmlI C'TyrA_G
Cuts at: 0 589 1734
Size: 589 1145
SphI G_CATG'C
Cuts at: 0 46 743 1734
Size: 46 697 991
Fragments arranged by size:
991 697 46
SrfI GCCC'GGGC
Cuts at: 0 631 1734
Size: 631 1103
StuI AGG'CCT
Cuts at: 0 460 1039 1063 1603 1734
Size: 460 579 24 540 131
Fragments arranged by size:
579 540 460 131 24
StyI C'CwwG_G
Cuts at: 0 455 873 1734
Size: 455 418 861
Fragments arranged by size:
861 455 418
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 597 1048 1522 1734
Size: 597 451 474 212
Fragments arranged by size:
597 474 451 212
TatI w'GTAC_w
Cuts at: 0 272 1298 1734
Size: 272 1026 436
Fragments arranged by size:
1026 436 272
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 245 277 770 802 1657 1689 1734
Size: 245 32 493 32 855 32 45
Fragments arranged by size:
855 493 245 45 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 766 912 1161 1683 1734
Size: 766 146 249 522 51
Fragments arranged by size:
766 522 249 146 51
XhoI C'TCGA_G
Cuts at: 0 589 1734
Size: 589 1145
XmaI C'CCGG_G
Cuts at: 0 629 1462 1734
Size: 629 833 272
Fragments arranged by size:
833 629 272
ZraI GAC'GTC
Cuts at: 0 102 1734
Size: 102 1632
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AhdI AlfI AlwNI
ApaLI AvaI BanI BanII BbeI BbsI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrFI BsrGI BssHII BstAPI BstXI BstYI BtgI
BtsI DraIII EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII KasI MspA1I NaeI NarI NgoMIV
NotI NspI PasI PflMI PfoI PmlI PpiI PpuMI
PshAI PspXI PstI PvuII SacI SexAI SfcI SfoI
SmaI SmlI SphI SrfI StuI StyI TaqII TatI
TstI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AleI AloI ApaI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BmtI Bpu10I BpuEI BsaI
BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EarI EciI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI NcoI NdeI NheI NruI NsiI PacI
PciI PmeI PsiI PspOMI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI
SnaBI SpeI SspI SwaI Tth111I XbaI XmnI