Restriction map for
pFN21AE4144
(Linear) (Six-base) MAPSORT of: KIEE4144.seq Check: 6302 from: 1 to: 1716
KIEE4144.orf
With 182 enzymes: SgfI *
July 3, 2012 18:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1341 1716
Size: 1341 375
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 1153 1716
Size: 326 827 563
Fragments arranged by size:
827 563 326
AfeI AGC'GCT
Cuts at: 0 27 1596 1716
Size: 27 1569 120
Fragments arranged by size:
1569 120 27
AhdI GACnn_n'nnGTC
Cuts at: 0 1660 1716
Size: 1660 56
AleI CACnn'nnGTG
Cuts at: 0 1426 1716
Size: 1426 290
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 104 1716
Size: 104 1612
AlwNI CAG_nnn'CTG
Cuts at: 0 319 1716
Size: 319 1397
ApaI G_GGCC'C
Cuts at: 0 916 1716
Size: 916 800
ApoI r'AATT_y
Cuts at: 0 241 329 1716
Size: 241 88 1387
Fragments arranged by size:
1387 241 88
AvrII C'CTAG_G
Cuts at: 0 781 1716
Size: 781 935
BanI G'GyrC_C
Cuts at: 0 1080 1716
Size: 1080 636
BanII G_rGCy'C
Cuts at: 0 916 1050 1372 1716
Size: 916 134 322 344
Fragments arranged by size:
916 344 322 134
BbsI GAAGACnn'nnnn_
Cuts at: 0 1568 1716
Size: 1568 148
BciVI GTATCCnnnnn_n'
Cuts at: 0 148 1716
Size: 148 1568
BclI T'GATC_A
Cuts at: 0 824 1716
Size: 824 892
BfrBI ATG'CAT
Cuts at: 0 652 1716
Size: 652 1064
Bme1580I G_kGCm'C
Cuts at: 0 916 1716
Size: 916 800
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1454 1716
Size: 1454 262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 1056 1319 1716
Size: 665 391 263 397
Fragments arranged by size:
665 397 391 263
BsaAI yAC'GTr
Cuts at: 0 1161 1716
Size: 1161 555
BsaHI Gr'CG_yC
Cuts at: 0 1058 1716
Size: 1058 658
BsaWI w'CCGG_w
Cuts at: 0 382 918 1372 1620 1716
Size: 382 536 454 248 96
Fragments arranged by size:
536 454 382 248 96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1344 1359 1374 1389 1716
Size: 1344 15 15 15 327
Fragments arranged by size:
1344 327 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 554 770 1592 1716
Size: 554 216 822 124
Fragments arranged by size:
822 554 216 124
BseYI C'CCAG_C
Cuts at: 0 1232 1460 1716
Size: 1232 228 256
Fragments arranged by size:
1232 256 228
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 52 1716
Size: 34 18 1664
Fragments arranged by size:
1664 34 18
BsiHKAI G_wGCw'C
Cuts at: 0 1296 1372 1716
Size: 1296 76 344
Fragments arranged by size:
1296 344 76
BsmBI CGTCTCn'nnnn_
Cuts at: 0 974 1716
Size: 974 742
Bsp1286I G_dGCh'C
Cuts at: 0 916 1050 1296 1372 1716
Size: 916 134 246 76 344
Fragments arranged by size:
916 344 246 134 76
BspEI T'CCGG_A
Cuts at: 0 918 1716
Size: 918 798
BspHI T'CATG_A
Cuts at: 0 590 1716
Size: 590 1126
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1211 1341 1491 1650 1716
Size: 1211 130 150 159 66
Fragments arranged by size:
1211 159 150 130 66
BsrBI CCG'CTC
Cuts at: 0 570 1716
Size: 570 1146
BsrDI GCAATG_nn'
Cuts at: 0 607 1716
Size: 607 1109
BstEII G'GTnAC_C
Cuts at: 0 193 1716
Size: 193 1523
BstYI r'GATC_y
Cuts at: 0 833 1716
Size: 833 883
Bsu36I CC'TnA_GG
Cuts at: 0 1604 1716
Size: 1604 112
BtgI C'CryG_G
Cuts at: 0 614 1026 1716
Size: 614 412 690
Fragments arranged by size:
690 614 412
DraI TTT'AAA
Cuts at: 0 657 1716
Size: 657 1059
EaeI y'GGCC_r
Cuts at: 0 1023 1247 1716
Size: 1023 224 469
Fragments arranged by size:
1023 469 224
EarI CTCTTCn'nnn_
Cuts at: 0 638 1008 1716
Size: 638 370 708
Fragments arranged by size:
708 638 370
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1716
Size: 19 1697
EcoICRI GAG'CTC
Cuts at: 0 1370 1716
Size: 1370 346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 665 1056 1153 1319 1716
Size: 326 339 391 97 166 397
Fragments arranged by size:
397 391 339 326 166 97
EcoO109I rG'GnC_Cy
Cuts at: 0 206 368 913 1503 1716
Size: 206 162 545 590 213
Fragments arranged by size:
590 545 213 206 162
EcoRI G'AATT_C
Cuts at: 0 241 1716
Size: 241 1475
HaeII r_GCGC'y
Cuts at: 0 29 1598 1716
Size: 29 1569 118
Fragments arranged by size:
1569 118 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 260 292 727 759 1202 1234 1646 1678
Size: 260 32 435 32 443 32 412 32
Cuts at: 1678 1716
Size: 38
Fragments arranged by size:
443 435 412 260 38 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 1716
Size: 246 1470
MscI TGG'CCA
Cuts at: 0 1249 1716
Size: 1249 467
MslI CAynn'nnrTG
Cuts at: 0 1426 1716
Size: 1426 290
MspA1I CmG'CkG
Cuts at: 0 440 1716
Size: 440 1276
NsiI A_TGCA'T
Cuts at: 0 654 1716
Size: 654 1062
NspI r_CATG'y
Cuts at: 0 937 1716
Size: 937 779
PasI CC'CwG_GG
Cuts at: 0 1455 1716
Size: 1455 261
PflMI CCAn_nnn'nTGG
Cuts at: 0 877 909 1716
Size: 877 32 807
Fragments arranged by size:
877 807 32
PfoI T'CCnGG_A
Cuts at: 0 491 596 1061 1716
Size: 491 105 465 655
Fragments arranged by size:
655 491 465 105
PmlI CAC'GTG
Cuts at: 0 1161 1716
Size: 1161 555
PpuMI rG'GwC_Cy
Cuts at: 0 1503 1716
Size: 1503 213
PshAI GACnn'nnGTC
Cuts at: 0 440 1716
Size: 440 1276
PspOMI G'GGCC_C
Cuts at: 0 912 1716
Size: 912 804
SacI G_AGCT'C
Cuts at: 0 1372 1716
Size: 1372 344
SfcI C'TryA_G
Cuts at: 0 1545 1716
Size: 1545 171
SphI G_CATG'C
Cuts at: 0 937 1716
Size: 937 779
SspI AAT'ATT
Cuts at: 0 1648 1716
Size: 1648 68
StuI AGG'CCT
Cuts at: 0 995 1555 1716
Size: 995 560 161
Fragments arranged by size:
995 560 161
StyI C'CwwG_G
Cuts at: 0 176 209 781 1363 1397 1716
Size: 176 33 572 582 34 319
Fragments arranged by size:
582 572 319 176 34 33
TatI w'GTAC_w
Cuts at: 0 162 375 660 1716
Size: 162 213 285 1056
Fragments arranged by size:
1056 285 213 162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 416 448 1487 1519 1716
Size: 416 32 1039 32 197
Fragments arranged by size:
1039 416 197 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1313 1716
Size: 1313 403
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 132 417 844 1716
Size: 132 285 427 872
Fragments arranged by size:
872 427 285 132
Enzymes that do cut:
AarI AcuI AfeI AhdI AleI AlfI AlwNI ApaI
ApoI AvrII BanI BanII BbsI BciVI BclI BfrBI
Bme1580I BplI BpmI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BstEII BstYI Bsu36I BtgI DraI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I
MmeI MscI MslI MspA1I NsiI NspI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI SacI SfcI SphI
SspI StuI StyI TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AloI
ApaLI AscI AseI AsiSI AvaI BaeI BamHI BbeI
BbvCI BcgI BglI BglII BlpI BmgBI BmrI BmtI
Bpu10I BpuEI BsaI BsaBI BsiEI BsiWI BsmI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgZI
BtsI ClaI CspCI DraIII DrdI EagI EcoNI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PciI PmeI PpiI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SwaI TaqII
XbaI XhoI XmaI XmnI ZraI