Restriction map for
pFN21AE4140
(Linear) (Six-base) MAPSORT of: KIEE4140.seq Check: 525 from: 1 to: 1671
KIEE4140.orf
With 182 enzymes: SgfI *
March 22, 2012 17:27 ..
AccI GT'mk_AC
Cuts at: 0 1602 1671
Size: 1602 69
Acc65I G'GTAC_C
Cuts at: 0 1238 1671
Size: 1238 433
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1393 1671
Size: 1393 278
AfeI AGC'GCT
Cuts at: 0 216 1671
Size: 216 1455
AflIII A'CryG_T
Cuts at: 0 1346 1671
Size: 1346 325
AleI CACnn'nnGTG
Cuts at: 0 1507 1671
Size: 1507 164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1128 1671
Size: 1128 543
AlwNI CAG_nnn'CTG
Cuts at: 0 1281 1371 1592 1671
Size: 1281 90 221 79
Fragments arranged by size:
1281 221 90 79
ApaI G_GGCC'C
Cuts at: 0 227 1671
Size: 227 1444
AseI AT'TA_AT
Cuts at: 0 491 1671
Size: 491 1180
AvaI C'yCGr_G
Cuts at: 0 148 1515 1671
Size: 148 1367 156
Fragments arranged by size:
1367 156 148
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1149 1182 1671
Size: 1149 33 489
Fragments arranged by size:
1149 489 33
BanI G'GyrC_C
Cuts at: 0 67 899 1238 1671
Size: 67 832 339 433
Fragments arranged by size:
832 433 339 67
BanII G_rGCy'C
Cuts at: 0 149 227 1671
Size: 149 78 1444
Fragments arranged by size:
1444 149 78
BbeI G_GCGC'C
Cuts at: 0 71 903 1671
Size: 71 832 768
Fragments arranged by size:
832 768 71
BbsI GAAGACnn'nnnn_
Cuts at: 0 95 1422 1671
Size: 95 1327 249
Fragments arranged by size:
1327 249 95
BciVI GTATCCnnnnn_n'
Cuts at: 0 358 1671
Size: 358 1313
BclI T'GATC_A
Cuts at: 0 743 1671
Size: 743 928
BglII A'GATC_T
Cuts at: 0 560 1488 1647 1671
Size: 560 928 159 24
Fragments arranged by size:
928 560 159 24
Bme1580I G_kGCm'C
Cuts at: 0 227 1671
Size: 227 1444
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1530 1671
Size: 1530 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 654 1671
Size: 481 173 1017
Fragments arranged by size:
1017 481 173
Bpu10I CC'TnA_GC
Cuts at: 0 403 1307 1671
Size: 403 904 364
Fragments arranged by size:
904 403 364
BsaHI Gr'CG_yC
Cuts at: 0 68 158 900 1671
Size: 68 90 742 771
Fragments arranged by size:
771 742 90 68
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 70 88 91 94 301 1342 1671
Size: 58 12 18 3 3 207 1041 329
Fragments arranged by size:
1041 329 207 58 18 12 3 3
BseYI C'CCAG_C
Cuts at: 0 153 1115 1671
Size: 153 962 556
Fragments arranged by size:
962 556 153
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 1384 1671
Size: 69 1315 287
Fragments arranged by size:
1315 287 69
BsiEI CG_ry'CG
Cuts at: 0 317 1671
Size: 317 1354
BsiHKAI G_wGCw'C
Cuts at: 0 311 1295 1514 1671
Size: 311 984 219 157
Fragments arranged by size:
984 311 219 157
BsmI GAATG_Cn'
Cuts at: 0 1503 1671
Size: 1503 168
BsmBI CGTCTCn'nnnn_
Cuts at: 0 187 1671
Size: 187 1484
Bsp1286I G_dGCh'C
Cuts at: 0 149 227 311 1295 1514 1671
Size: 149 78 84 984 219 157
Fragments arranged by size:
984 219 157 149 84 78
BsrBI CCG'CTC
Cuts at: 0 171 1671
Size: 171 1500
BsrGI T'GTAC_A
Cuts at: 0 1418 1671
Size: 1418 253
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1204 1281 1671
Size: 1204 77 390
Fragments arranged by size:
1204 390 77
BstXI CCAn_nnnn'nTGG
Cuts at: 0 660 1671
Size: 660 1011
BstYI r'GATC_y
Cuts at: 0 120 560 1147 1488 1647 1671
Size: 120 440 587 341 159 24
Fragments arranged by size:
587 440 341 159 120 24
BtgI C'CryG_G
Cuts at: 0 1132 1671
Size: 1132 539
BtsI GCAGTG_nn'
Cuts at: 0 57 634 1671
Size: 57 577 1037
Fragments arranged by size:
1037 577 57
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 397 432 1671
Size: 397 35 1239
Fragments arranged by size:
1239 397 35
DraIII CAC_nnn'GTG
Cuts at: 0 339 1671
Size: 339 1332
EaeI y'GGCC_r
Cuts at: 0 314 1671
Size: 314 1357
EagI C'GGCC_G
Cuts at: 0 314 1671
Size: 314 1357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 654 1393 1671
Size: 481 173 739 278
Fragments arranged by size:
739 481 278 173
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1375 1671
Size: 1375 296
EcoO109I rG'GnC_Cy
Cuts at: 0 223 1671
Size: 223 1448
EcoRV GAT'ATC
Cuts at: 0 711 1671
Size: 711 960
HaeII r_GCGC'y
Cuts at: 0 71 218 903 1671
Size: 71 147 685 768
Fragments arranged by size:
768 685 147 71
HincII GTy'rAC
Cuts at: 0 360 1603 1671
Size: 360 1243 68
Fragments arranged by size:
1243 360 68
KasI G'GCGC_C
Cuts at: 0 67 899 1671
Size: 67 832 772
Fragments arranged by size:
832 772 67
KpnI G_GTAC'C
Cuts at: 0 1242 1671
Size: 1242 429
MfeI C'AATT_G
Cuts at: 0 414 1002 1049 1671
Size: 414 588 47 622
Fragments arranged by size:
622 588 414 47
MslI CAynn'nnrTG
Cuts at: 0 704 733 1414 1507 1628 1671
Size: 704 29 681 93 121 43
Fragments arranged by size:
704 681 121 93 43 29
MspA1I CmG'CkG
Cuts at: 0 65 1671
Size: 65 1606
NarI GG'CG_CC
Cuts at: 0 68 900 1671
Size: 68 832 771
Fragments arranged by size:
832 771 68
NcoI C'CATG_G
Cuts at: 0 1132 1671
Size: 1132 539
NspI r_CATG'y
Cuts at: 0 1275 1350 1627 1671
Size: 1275 75 277 44
Fragments arranged by size:
1275 277 75 44
PciI A'CATG_T
Cuts at: 0 1346 1671
Size: 1346 325
PfoI T'CCnGG_A
Cuts at: 0 350 1351 1671
Size: 350 1001 320
Fragments arranged by size:
1001 350 320
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1351 1383 1671
Size: 1351 32 288
Fragments arranged by size:
1351 288 32
PspOMI G'GGCC_C
Cuts at: 0 223 1671
Size: 223 1448
PstI C_TGCA'G
Cuts at: 0 1235 1556 1671
Size: 1235 321 115
Fragments arranged by size:
1235 321 115
SalI G'TCGA_C
Cuts at: 0 1601 1671
Size: 1601 70
SfcI C'TryA_G
Cuts at: 0 1231 1552 1671
Size: 1231 321 119
Fragments arranged by size:
1231 321 119
SfoI GGC'GCC
Cuts at: 0 69 901 1671
Size: 69 832 770
Fragments arranged by size:
832 770 69
SmaI CCC'GGG
Cuts at: 0 150 1671
Size: 150 1521
SrfI GCCC'GGGC
Cuts at: 0 150 1671
Size: 150 1521
SspI AAT'ATT
Cuts at: 0 643 1671
Size: 643 1028
StyI C'CwwG_G
Cuts at: 0 1132 1671
Size: 1132 539
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1107 1671
Size: 1107 564
TatI w'GTAC_w
Cuts at: 0 1418 1572 1583 1658 1671
Size: 1418 154 11 75 13
Fragments arranged by size:
1418 154 75 13 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 63 1333 1365 1671
Size: 31 32 1270 32 306
Fragments arranged by size:
1270 306 32 32 31
XbaI T'CTAG_A
Cuts at: 0 1491 1671
Size: 1491 180
XmaI C'CCGG_G
Cuts at: 0 148 1671
Size: 148 1523
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AleI AlfI AlwNI
ApaI AseI AvaI BaeI BanI BanII BbeI BbsI
BciVI BclI BglII Bme1580I BplI BpmI Bpu10I BsaHI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BsrBI BsrGI BstAPI BstXI BstYI BtgI BtsI CspCI
DraIII EaeI EagI Eco57MI EcoNI EcoO109I EcoRV HaeII
HincII KasI KpnI MfeI MslI MspA1I NarI NcoI
NspI PciI PfoI PpiI PspOMI PstI SalI SfcI
SfoI SmaI SrfI SspI StyI TaqII TatI TstI
XbaI XmaI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AloI ApaLI
ApoI AscI AsiSI AvrII BamHI BbvCI BcgI BfrBI
BglI BlpI BmgBI BmrI BmtI BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BsiWI BspEI BspHI BspMI BsrDI
BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI DraI DrdI EarI EciI EcoICRI EcoRI FalI
FseI FspI FspAI Hin4I HindIII HpaI MluI MmeI
MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PpuMI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI StuI SwaI Tth111I XcmI XhoI
XmnI ZraI