Restriction map for
pFN21AE4137
(Linear) (Six-base) MAPSORT of: KIEE4137.seq Check: 5562 from: 1 to: 1356
KIEE4137.orf
With 182 enzymes: SgfI *
July 3, 2012 18:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 436 595 1218 1356
Size: 436 159 623 138
Fragments arranged by size:
623 436 159 138
AccI GT'mk_AC
Cuts at: 0 243 1356
Size: 243 1113
Acc65I G'GTAC_C
Cuts at: 0 199 1356
Size: 199 1157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 1112 1356
Size: 268 844 244
Fragments arranged by size:
844 268 244
AflIII A'CryG_T
Cuts at: 0 726 1356
Size: 726 630
AleI CACnn'nnGTG
Cuts at: 0 487 1356
Size: 487 869
ApaLI G'TGCA_C
Cuts at: 0 452 1356
Size: 452 904
ApoI r'AATT_y
Cuts at: 0 794 1356
Size: 794 562
BanI G'GyrC_C
Cuts at: 0 199 1356
Size: 199 1157
BanII G_rGCy'C
Cuts at: 0 515 1136 1356
Size: 515 621 220
Fragments arranged by size:
621 515 220
BbsI GAAGACnn'nnnn_
Cuts at: 0 587 1356
Size: 587 769
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 212 246 579 613 1356
Size: 212 34 333 34 743
Fragments arranged by size:
743 333 212 34 34
BclI T'GATC_A
Cuts at: 0 566 740 756 1356
Size: 566 174 16 600
Fragments arranged by size:
600 566 174 16
BglI GCCn_nnn'nGGC
Cuts at: 0 1165 1356
Size: 1165 191
Bme1580I G_kGCm'C
Cuts at: 0 456 1170 1356
Size: 456 714 186
Fragments arranged by size:
714 456 186
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 26 789 1356
Size: 26 763 567
Fragments arranged by size:
763 567 26
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1169 1181 1243 1262 1356
Size: 1169 12 62 19 94
Fragments arranged by size:
1169 94 62 19 12
BsaAI yAC'GTr
Cuts at: 0 964 1356
Size: 964 392
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1043 1073 1356
Size: 1043 30 283
Fragments arranged by size:
1043 283 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 209 400 682 727 1064 1241 1356
Size: 209 191 282 45 337 177 115
Fragments arranged by size:
337 282 209 191 177 115 45
BsiEI CG_ry'CG
Cuts at: 0 552 1356
Size: 552 804
BsiHKAI G_wGCw'C
Cuts at: 0 456 515 738 1136 1356
Size: 456 59 223 398 220
Fragments arranged by size:
456 398 223 220 59
BsmI GAATG_Cn'
Cuts at: 0 843 1356
Size: 843 513
BsmBI CGTCTCn'nnnn_
Cuts at: 0 777 1356
Size: 777 579
Bsp1286I G_dGCh'C
Cuts at: 0 456 515 738 1136 1170 1356
Size: 456 59 223 398 34 186
Fragments arranged by size:
456 398 223 186 59 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 302 436 595 1218 1356
Size: 302 134 159 623 138
Fragments arranged by size:
623 302 159 138 134
BssSI C'ACGA_G
Cuts at: 0 535 1356
Size: 535 821
BstXI CCAn_nnnn'nTGG
Cuts at: 0 537 1356
Size: 537 819
BtgI C'CryG_G
Cuts at: 0 47 206 1356
Size: 47 159 1150
Fragments arranged by size:
1150 159 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 510 1356
Size: 510 846
BtsI GCAGTG_nn'
Cuts at: 0 830 1296 1356
Size: 830 466 60
Fragments arranged by size:
830 466 60
EarI CTCTTCn'nnn_
Cuts at: 0 377 1064 1356
Size: 377 687 292
Fragments arranged by size:
687 377 292
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1028 1356
Size: 1028 328
EcoICRI GAG'CTC
Cuts at: 0 513 1134 1356
Size: 513 621 222
Fragments arranged by size:
621 513 222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 1112 1169 1181 1243 1262 1356
Size: 268 844 57 12 62 19 94
Fragments arranged by size:
844 268 94 62 57 19 12
EcoRI G'AATT_C
Cuts at: 0 794 1356
Size: 794 562
HincII GTy'rAC
Cuts at: 0 936 1356
Size: 936 420
HindIII A'AGCT_T
Cuts at: 0 1124 1356
Size: 1124 232
KpnI G_GTAC'C
Cuts at: 0 203 1356
Size: 203 1153
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 1356
Size: 380 976
MslI CAynn'nnrTG
Cuts at: 0 487 535 731 908 1356
Size: 487 48 196 177 448
Fragments arranged by size:
487 448 196 177 48
MspA1I CmG'CkG
Cuts at: 0 230 576 1356
Size: 230 346 780
Fragments arranged by size:
780 346 230
NcoI C'CATG_G
Cuts at: 0 47 206 1356
Size: 47 159 1150
Fragments arranged by size:
1150 159 47
NspI r_CATG'y
Cuts at: 0 730 1356
Size: 730 626
PasI CC'CwG_GG
Cuts at: 0 1162 1356
Size: 1162 194
PciI A'CATG_T
Cuts at: 0 726 1356
Size: 726 630
PflMI CCAn_nnn'nTGG
Cuts at: 0 1285 1356
Size: 1285 71
PfoI T'CCnGG_A
Cuts at: 0 1098 1356
Size: 1098 258
PshAI GACnn'nnGTC
Cuts at: 0 504 691 1356
Size: 504 187 665
Fragments arranged by size:
665 504 187
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1067 1099 1356
Size: 1067 32 257
Fragments arranged by size:
1067 257 32
PstI C_TGCA'G
Cuts at: 0 836 1154 1275 1356
Size: 836 318 121 81
Fragments arranged by size:
836 318 121 81
PvuI CG_AT'CG
Cuts at: 0 552 1356
Size: 552 804
PvuII CAG'CTG
Cuts at: 0 576 1356
Size: 576 780
SacI G_AGCT'C
Cuts at: 0 515 1136 1356
Size: 515 621 220
Fragments arranged by size:
621 515 220
SfcI C'TryA_G
Cuts at: 0 240 832 1150 1271 1356
Size: 240 592 318 121 85
Fragments arranged by size:
592 318 240 121 85
StuI AGG'CCT
Cuts at: 0 169 1356
Size: 169 1187
StyI C'CwwG_G
Cuts at: 0 47 206 1356
Size: 47 159 1150
Fragments arranged by size:
1150 159 47
XmnI GAAnn'nnTTC
Cuts at: 0 925 1082 1356
Size: 925 157 274
Fragments arranged by size:
925 274 157
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI ApaLI ApoI
BanI BanII BbsI BcgI BclI BglI Bme1580I BplI
BpmI BsaAI BsaXI BseRI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BssSI BstXI BtgI BtgZI BtsI EarI
EciI EcoICRI Eco57MI EcoRI HincII HindIII KpnI MmeI
MslI MspA1I NcoI NspI PasI PciI PflMI PfoI
PshAI PsrI PstI PvuI PvuII SacI SfcI StuI
StyI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AlfI AloI
AlwNI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BbvCI BciVI BfrBI BglII BlpI BmgBI
BmrI BmtI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BseYI BsgI BsiWI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EaeI EagI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HaeII Hin4I
HpaI KasI MfeI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI
PpiI PpuMI PsiI PspOMI PspXI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI