Restriction map for
pFN21AE4136
(Linear) (Six-base) MAPSORT of: KIEE4136.seq Check: 7099 from: 1 to: 1641
KIEE4136.orf
With 182 enzymes: SgfI *
March 22, 2012 17:27 ..
AccI GT'mk_AC
Cuts at: 0 998 1641
Size: 998 643
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 202 1063 1460 1641
Size: 43 159 861 397 181
Fragments arranged by size:
861 397 181 159 43
AflII C'TTAA_G
Cuts at: 0 750 1641
Size: 750 891
AflIII A'CryG_T
Cuts at: 0 932 1641
Size: 932 709
AgeI A'CCGG_T
Cuts at: 0 427 1641
Size: 427 1214
AhdI GACnn_n'nnGTC
Cuts at: 0 1102 1641
Size: 1102 539
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 212 1641
Size: 212 1429
AlwNI CAG_nnn'CTG
Cuts at: 0 565 615 993 1641
Size: 565 50 378 648
Fragments arranged by size:
648 565 378 50
ApaLI G'TGCA_C
Cuts at: 0 1382 1641
Size: 1382 259
ApoI r'AATT_y
Cuts at: 0 235 629 814 1641
Size: 235 394 185 827
Fragments arranged by size:
827 394 235 185
AvaI C'yCGr_G
Cuts at: 0 527 1445 1598 1641
Size: 527 918 153 43
Fragments arranged by size:
918 527 153 43
BamHI G'GATC_C
Cuts at: 0 661 1641
Size: 661 980
BanI G'GyrC_C
Cuts at: 0 1281 1641
Size: 1281 360
BanII G_rGCy'C
Cuts at: 0 114 255 518 600 1641
Size: 114 141 263 82 1041
Fragments arranged by size:
1041 263 141 114 82
BbsI GAAGACnn'nnnn_
Cuts at: 0 596 1641
Size: 596 1045
BclI T'GATC_A
Cuts at: 0 819 1215 1641
Size: 819 396 426
Fragments arranged by size:
819 426 396
BlpI GC'TnA_GC
Cuts at: 0 581 1310 1641
Size: 581 729 331
Fragments arranged by size:
729 581 331
Bme1580I G_kGCm'C
Cuts at: 0 39 46 1386 1598 1641
Size: 39 7 1340 212 43
Fragments arranged by size:
1340 212 43 39 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 1272 1641
Size: 1272 369
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 1051 1641
Size: 193 858 590
Fragments arranged by size:
858 590 193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 1386 1641
Size: 865 521 255
Fragments arranged by size:
865 521 255
BsaBI GATnn'nnATC
Cuts at: 0 848 1641
Size: 848 793
BsaHI Gr'CG_yC
Cuts at: 0 549 1622 1641
Size: 549 1073 19
Fragments arranged by size:
1073 549 19
BsaWI w'CCGG_w
Cuts at: 0 427 1566 1641
Size: 427 1139 75
Fragments arranged by size:
1139 427 75
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 122 868 1510 1641
Size: 122 746 642 131
Fragments arranged by size:
746 642 131 122
BseYI C'CCAG_C
Cuts at: 0 1206 1641
Size: 1206 435
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 808 857 1641
Size: 578 230 49 784
Fragments arranged by size:
784 578 230 49
BsiEI CG_ry'CG
Cuts at: 0 132 1641
Size: 132 1509
BsiHKAI G_wGCw'C
Cuts at: 0 255 1386 1641
Size: 255 1131 255
Fragments arranged by size:
1131 255 255
BsmI GAATG_Cn'
Cuts at: 0 11 1641
Size: 11 1630
Bsp1286I G_dGCh'C
Cuts at: 0 39 46 114 255 518 600 1386 1598
Size: 39 7 68 141 263 82 786 212
Cuts at: 1598 1641
Size: 43
Fragments arranged by size:
786 263 212 141 82 68 43 39
7
BspEI T'CCGG_A
Cuts at: 0 1566 1641
Size: 1566 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 402 1293 1641
Size: 402 891 348
Fragments arranged by size:
891 402 348
BsrBI CCG'CTC
Cuts at: 0 1549 1641
Size: 1549 92
BsrDI GCAATG_nn'
Cuts at: 0 1582 1641
Size: 1582 59
BsrFI r'CCGG_y
Cuts at: 0 427 1641
Size: 427 1214
BsrGI T'GTAC_A
Cuts at: 0 929 1641
Size: 929 712
BssSI C'ACGA_G
Cuts at: 0 211 1641
Size: 211 1430
BstAPI GCAn_nnn'nTGC
Cuts at: 0 615 795 1641
Size: 615 180 846
Fragments arranged by size:
846 615 180
BstEII G'GTnAC_C
Cuts at: 0 1552 1641
Size: 1552 89
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1134 1641
Size: 1134 507
BstYI r'GATC_y
Cuts at: 0 661 1150 1373 1641
Size: 661 489 223 268
Fragments arranged by size:
661 489 268 223
BtgI C'CryG_G
Cuts at: 0 543 1641
Size: 543 1098
BtsI GCAGTG_nn'
Cuts at: 0 566 764 1517 1641
Size: 566 198 753 124
Fragments arranged by size:
753 566 198 124
ClaI AT'CG_AT
Cuts at: 0 151 1641
Size: 151 1490
DraI TTT'AAA
Cuts at: 0 959 1641
Size: 959 682
EaeI y'GGCC_r
Cuts at: 0 215 477 645 1641
Size: 215 262 168 996
Fragments arranged by size:
996 262 215 168
EarI CTCTTCn'nnn_
Cuts at: 0 347 1279 1394 1641
Size: 347 932 115 247
Fragments arranged by size:
932 347 247 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 66 522 1641
Size: 66 456 1119
Fragments arranged by size:
1119 456 66
EcoICRI GAG'CTC
Cuts at: 0 253 1641
Size: 253 1388
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 193 202 1051 1063 1460 1641
Size: 43 150 9 849 12 397 181
Fragments arranged by size:
849 397 181 150 43 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 352 1276 1641
Size: 352 924 365
Fragments arranged by size:
924 365 352
EcoO109I rG'GnC_Cy
Cuts at: 0 357 1047 1641
Size: 357 690 594
Fragments arranged by size:
690 594 357
EcoRI G'AATT_C
Cuts at: 0 235 1641
Size: 235 1406
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1490 1641
Size: 1490 151
HaeII r_GCGC'y
Cuts at: 0 492 1641
Size: 492 1149
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 212 244 669 701 1088 1120 1142 1174
Size: 212 32 425 32 387 32 22 32
Cuts at: 1174 1641
Size: 467
Fragments arranged by size:
467 425 387 212 32 32 32 32
22
MfeI C'AATT_G
Cuts at: 0 641 1082 1641
Size: 641 441 559
Fragments arranged by size:
641 559 441
MscI TGG'CCA
Cuts at: 0 217 647 1641
Size: 217 430 994
Fragments arranged by size:
994 430 217
MslI CAynn'nnrTG
Cuts at: 0 1393 1535 1641
Size: 1393 142 106
Fragments arranged by size:
1393 142 106
MspA1I CmG'CkG
Cuts at: 0 1504 1641
Size: 1504 137
NspI r_CATG'y
Cuts at: 0 936 1641
Size: 936 705
PciI A'CATG_T
Cuts at: 0 932 1641
Size: 932 709
PfoI T'CCnGG_A
Cuts at: 0 1192 1641
Size: 1192 449
PpuMI rG'GwC_Cy
Cuts at: 0 357 1641
Size: 357 1284
PstI C_TGCA'G
Cuts at: 0 611 620 989 1641
Size: 611 9 369 652
Fragments arranged by size:
652 611 369 9
PvuII CAG'CTG
Cuts at: 0 1504 1641
Size: 1504 137
SacI G_AGCT'C
Cuts at: 0 255 1641
Size: 255 1386
SfcI C'TryA_G
Cuts at: 0 607 616 985 1641
Size: 607 9 369 656
Fragments arranged by size:
656 607 369 9
SmaI CCC'GGG
Cuts at: 0 529 1447 1600 1641
Size: 529 918 153 41
Fragments arranged by size:
918 529 153 41
SmlI C'TyrA_G
Cuts at: 0 750 880 1365 1641
Size: 750 130 485 276
Fragments arranged by size:
750 485 276 130
SrfI GCCC'GGGC
Cuts at: 0 1447 1641
Size: 1447 194
StyI C'CwwG_G
Cuts at: 0 278 1139 1641
Size: 278 861 502
Fragments arranged by size:
861 502 278
TatI w'GTAC_w
Cuts at: 0 929 1641
Size: 929 712
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 446 478 1641
Size: 446 32 1163
Fragments arranged by size:
1163 446 32
XmaI C'CCGG_G
Cuts at: 0 527 1445 1598 1641
Size: 527 918 153 43
Fragments arranged by size:
918 527 153 43
Enzymes that do cut:
AccI AcuI AflII AflIII AgeI AhdI AlfI AlwNI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BclI
BlpI Bme1580I BmrI BpmI BpuEI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII
BstXI BstYI BtgI BtsI ClaI DraI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I MfeI MscI MslI MspA1I NspI PciI PfoI
PpuMI PstI PvuII SacI SfcI SmaI SmlI SrfI
StyI TatI TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AleI AloI ApaI
AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI
BciVI BfrBI BglI BglII BmgBI BmtI BplI Bpu10I
BsaI BsaAI BsaXI BsiWI BsmBI BspHI BssHII BstBI
BstZ17I Bsu36I BtgZI CspCI DraIII DrdI EagI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SspI StuI
SwaI TaqII Tth111I XbaI XcmI XhoI XmnI ZraI