Restriction map for
pFN21AE4133
(Linear) (Six-base) MAPSORT of: KIEE4133.seq Check: 8701 from: 1 to: 1614
KIEE4133.orf
With 182 enzymes: SgfI *
March 22, 2012 17:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 447 1614
Size: 447 1167
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 1174 1469 1614
Size: 248 926 295 145
Fragments arranged by size:
926 295 248 145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 469 1614
Size: 469 1145
AlwNI CAG_nnn'CTG
Cuts at: 0 56 145 183 808 1614
Size: 56 89 38 625 806
Fragments arranged by size:
806 625 89 56 38
ApaI G_GGCC'C
Cuts at: 0 1347 1614
Size: 1347 267
ApaLI G'TGCA_C
Cuts at: 0 555 1614
Size: 555 1059
AvaI C'yCGr_G
Cuts at: 0 391 1614
Size: 391 1223
BanI G'GyrC_C
Cuts at: 0 1396 1614
Size: 1396 218
BanII G_rGCy'C
Cuts at: 0 172 1347 1364 1614
Size: 172 1175 17 250
Fragments arranged by size:
1175 250 172 17
BglI GCCn_nnn'nGGC
Cuts at: 0 406 1093 1614
Size: 406 687 521
Fragments arranged by size:
687 521 406
Bme1580I G_kGCm'C
Cuts at: 0 348 559 1347 1614
Size: 348 211 788 267
Fragments arranged by size:
788 348 267 211
BmrI ACTGGGnnnn_n'
Cuts at: 0 29 827 1614
Size: 29 798 787
Fragments arranged by size:
798 787 29
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 851 1614
Size: 851 763
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 873 1614
Size: 262 611 741
Fragments arranged by size:
741 611 262
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 1015 1614
Size: 364 651 599
Fragments arranged by size:
651 599 364
BsaAI yAC'GTr
Cuts at: 0 612 1614
Size: 612 1002
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 85 115 1076 1106 1114 1144 1614
Size: 85 30 961 30 8 30 470
Fragments arranged by size:
961 470 85 30 30 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 110 379 442 484 1180 1614
Size: 110 269 63 42 696 434
Fragments arranged by size:
696 434 269 110 63 42
BseYI C'CCAG_C
Cuts at: 0 124 483 522 1346 1454 1614
Size: 124 359 39 824 108 160
Fragments arranged by size:
824 359 160 124 108 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 647 1614
Size: 475 172 967
Fragments arranged by size:
967 475 172
BsiHKAI G_wGCw'C
Cuts at: 0 172 559 817 1364 1614
Size: 172 387 258 547 250
Fragments arranged by size:
547 387 258 250 172
BsmI GAATG_Cn'
Cuts at: 0 1527 1614
Size: 1527 87
BsmBI CGTCTCn'nnnn_
Cuts at: 0 280 1614
Size: 280 1334
Bsp1286I G_dGCh'C
Cuts at: 0 172 348 559 817 1347 1364 1614
Size: 172 176 211 258 530 17 250
Fragments arranged by size:
530 258 250 211 176 172 17
BspHI T'CATG_A
Cuts at: 0 1220 1614
Size: 1220 394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 447 1570 1614
Size: 447 1123 44
Fragments arranged by size:
1123 447 44
BstAPI GCAn_nnn'nTGC
Cuts at: 0 183 1614
Size: 183 1431
BstBI TT'CG_AA
Cuts at: 0 1179 1614
Size: 1179 435
BstEII G'GTnAC_C
Cuts at: 0 292 1044 1614
Size: 292 752 570
Fragments arranged by size:
752 570 292
BstXI CCAn_nnnn'nTGG
Cuts at: 0 684 1316 1614
Size: 684 632 298
Fragments arranged by size:
684 632 298
BstYI r'GATC_y
Cuts at: 0 903 1614
Size: 903 711
Bsu36I CC'TnA_GG
Cuts at: 0 298 362 752 1238 1614
Size: 298 64 390 486 376
Fragments arranged by size:
486 390 376 298 64
BtgI C'CryG_G
Cuts at: 0 419 1614
Size: 419 1195
BtsI GCAGTG_nn'
Cuts at: 0 221 1614
Size: 221 1393
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1234 1269 1614
Size: 1234 35 345
Fragments arranged by size:
1234 345 35
DraI TTT'AAA
Cuts at: 0 1146 1614
Size: 1146 468
DraIII CAC_nnn'GTG
Cuts at: 0 933 1614
Size: 933 681
EarI CTCTTCn'nnn_
Cuts at: 0 28 419 791 1283 1490 1614
Size: 28 391 372 492 207 124
Fragments arranged by size:
492 391 372 207 124 28
EcoICRI GAG'CTC
Cuts at: 0 170 1362 1614
Size: 170 1192 252
Fragments arranged by size:
1192 252 170
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 262 873 1174 1469 1614
Size: 248 14 611 301 295 145
Fragments arranged by size:
611 301 295 248 145 14
EcoO109I rG'GnC_Cy
Cuts at: 0 502 805 1614
Size: 502 303 809
Fragments arranged by size:
809 502 303
EcoRV GAT'ATC
Cuts at: 0 1192 1614
Size: 1192 422
HaeII r_GCGC'y
Cuts at: 0 1353 1614
Size: 1353 261
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 629 661 1202 1234 1369 1401 1564 1596
Size: 629 32 541 32 135 32 163 32
Cuts at: 1596 1614
Size: 18
Fragments arranged by size:
629 541 163 135 32 32 32 32
18
MslI CAynn'nnrTG
Cuts at: 0 139 1376 1614
Size: 139 1237 238
Fragments arranged by size:
1237 238 139
MspA1I CmG'CkG
Cuts at: 0 201 449 483 696 772 1614
Size: 201 248 34 213 76 842
Fragments arranged by size:
842 248 213 201 76 34
NspI r_CATG'y
Cuts at: 0 190 543 762 1614
Size: 190 353 219 852
Fragments arranged by size:
852 353 219 190
PasI CC'CwG_GG
Cuts at: 0 105 550 808 1614
Size: 105 445 258 806
Fragments arranged by size:
806 445 258 105
PflMI CCAn_nnn'nTGG
Cuts at: 0 724 1614
Size: 724 890
PfoI T'CCnGG_A
Cuts at: 0 278 1614
Size: 278 1336
PshAI GACnn'nnGTC
Cuts at: 0 1247 1614
Size: 1247 367
PspOMI G'GGCC_C
Cuts at: 0 1343 1614
Size: 1343 271
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 623 655 1614
Size: 623 32 959
Fragments arranged by size:
959 623 32
PvuII CAG'CTG
Cuts at: 0 483 1614
Size: 483 1131
SacI G_AGCT'C
Cuts at: 0 172 1364 1614
Size: 172 1192 250
Fragments arranged by size:
1192 250 172
SmaI CCC'GGG
Cuts at: 0 393 1614
Size: 393 1221
SmlI C'TyrA_G
Cuts at: 0 379 1030 1614
Size: 379 651 584
Fragments arranged by size:
651 584 379
SspI AAT'ATT
Cuts at: 0 999 1614
Size: 999 615
StuI AGG'CCT
Cuts at: 0 1151 1614
Size: 1151 463
StyI C'CwwG_G
Cuts at: 0 791 1614
Size: 791 823
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 280 1308 1614
Size: 280 1028 306
Fragments arranged by size:
1028 306 280
TatI w'GTAC_w
Cuts at: 0 630 909 1614
Size: 630 279 705
Fragments arranged by size:
705 630 279
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 415 447 1076 1108 1614
Size: 415 32 629 32 506
Fragments arranged by size:
629 506 415 32 32
XmaI C'CCGG_G
Cuts at: 0 391 1614
Size: 391 1223
XmnI GAAnn'nnTTC
Cuts at: 0 1582 1614
Size: 1582 32
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaI ApaLI AvaI BanI
BanII BglI Bme1580I BmrI BplI BpmI BpuEI BsaAI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtsI CspCI DraI DraIII EarI EcoICRI Eco57MI
EcoO109I EcoRV HaeII Hin4I MslI MspA1I NspI PasI
PflMI PfoI PshAI PspOMI PsrI PvuII SacI SmaI
SmlI SspI StuI StyI TaqII TatI TstI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI Bpu10I BsaI BsaBI
BsaHI BsaWI BsiEI BsiWI BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstZ17I BtgZI ClaI DrdI EaeI
EagI EciI EcoNI EcoRI FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI
PsiI PspXI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI
XcmI XhoI ZraI