Restriction map for
pFN21AE4131
(Linear) (Six-base) MAPSORT of: KIEE4131.seq Check: 5396 from: 1 to: 1425
KIEE4131.orf
With 182 enzymes: SgfI *
July 3, 2012 18:37 ..
AccI GT'mk_AC
Cuts at: 0 155 1425
Size: 155 1270
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 1122 1373 1425
Size: 655 467 251 52
Fragments arranged by size:
655 467 251 52
AfeI AGC'GCT
Cuts at: 0 140 1425
Size: 140 1285
AflIII A'CryG_T
Cuts at: 0 1092 1425
Size: 1092 333
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 103 1425
Size: 103 1322
AlwNI CAG_nnn'CTG
Cuts at: 0 90 1425
Size: 90 1335
ApoI r'AATT_y
Cuts at: 0 1119 1425
Size: 1119 306
BanII G_rGCy'C
Cuts at: 0 7 1425
Size: 7 1418
BbsI GAAGACnn'nnnn_
Cuts at: 0 324 1425
Size: 324 1101
BclI T'GATC_A
Cuts at: 0 897 1425
Size: 897 528
BfrBI ATG'CAT
Cuts at: 0 872 1425
Size: 872 553
BglII A'GATC_T
Cuts at: 0 1317 1425
Size: 1317 108
Bme1580I G_kGCm'C
Cuts at: 0 1038 1236 1425
Size: 1038 198 189
Fragments arranged by size:
1038 198 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 112 1219 1425
Size: 54 58 1107 206
Fragments arranged by size:
1107 206 58 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 1425
Size: 209 1216
BsaBI GATnn'nnATC
Cuts at: 0 176 1425
Size: 176 1249
BsaWI w'CCGG_w
Cuts at: 0 433 917 1425
Size: 433 484 508
Fragments arranged by size:
508 484 433
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 420 1425
Size: 420 1005
BseYI C'CCAG_C
Cuts at: 0 343 450 998 1425
Size: 343 107 548 427
Fragments arranged by size:
548 427 343 107
BsiHKAI G_wGCw'C
Cuts at: 0 7 1425
Size: 7 1418
Bsp1286I G_dGCh'C
Cuts at: 0 7 1038 1236 1425
Size: 7 1031 198 189
Fragments arranged by size:
1031 198 189 7
BspEI T'CCGG_A
Cuts at: 0 433 1425
Size: 433 992
BspHI T'CATG_A
Cuts at: 0 483 1425
Size: 483 942
BstYI r'GATC_y
Cuts at: 0 122 510 1317 1425
Size: 122 388 807 108
Fragments arranged by size:
807 388 122 108
BstZ17I GTA'TAC
Cuts at: 0 156 1425
Size: 156 1269
Bsu36I CC'TnA_GG
Cuts at: 0 337 1425
Size: 337 1088
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 849 1425
Size: 849 576
DraI TTT'AAA
Cuts at: 0 624 713 1425
Size: 624 89 712
Fragments arranged by size:
712 624 89
EaeI y'GGCC_r
Cuts at: 0 957 1425
Size: 957 468
EarI CTCTTCn'nnn_
Cuts at: 0 1055 1425
Size: 1055 370
EcoICRI GAG'CTC
Cuts at: 0 5 1425
Size: 5 1420
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 112 655 1122 1219 1373 1425
Size: 54 58 543 467 97 154 52
Fragments arranged by size:
543 467 154 97 58 54 52
EcoO109I rG'GnC_Cy
Cuts at: 0 280 1425
Size: 280 1145
FspI TGC'GCA
Cuts at: 0 1343 1425
Size: 1343 82
HaeII r_GCGC'y
Cuts at: 0 142 1425
Size: 142 1283
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1302 1334 1425
Size: 1302 32 91
Fragments arranged by size:
1302 91 32
HincII GTy'rAC
Cuts at: 0 1155 1425
Size: 1155 270
MslI CAynn'nnrTG
Cuts at: 0 85 488 1271 1425
Size: 85 403 783 154
Fragments arranged by size:
783 403 154 85
MspA1I CmG'CkG
Cuts at: 0 21 1069 1425
Size: 21 1048 356
Fragments arranged by size:
1048 356 21
NdeI CA'TA_TG
Cuts at: 0 1268 1425
Size: 1268 157
NsiI A_TGCA'T
Cuts at: 0 874 1425
Size: 874 551
NspI r_CATG'y
Cuts at: 0 872 1096 1425
Size: 872 224 329
Fragments arranged by size:
872 329 224
PacI TTA_AT'TAA
Cuts at: 0 1394 1425
Size: 1394 31
PciI A'CATG_T
Cuts at: 0 1092 1425
Size: 1092 333
PflMI CCAn_nnn'nTGG
Cuts at: 0 238 1425
Size: 238 1187
PfoI T'CCnGG_A
Cuts at: 0 744 1425
Size: 744 681
PsiI TTA'TAA
Cuts at: 0 953 1425
Size: 953 472
PstI C_TGCA'G
Cuts at: 0 941 1325 1425
Size: 941 384 100
Fragments arranged by size:
941 384 100
PvuII CAG'CTG
Cuts at: 0 1069 1425
Size: 1069 356
SacI G_AGCT'C
Cuts at: 0 7 1425
Size: 7 1418
SfcI C'TryA_G
Cuts at: 0 218 585 937 1321 1425
Size: 218 367 352 384 104
Fragments arranged by size:
384 367 352 218 104
SmlI C'TyrA_G
Cuts at: 0 224 1425
Size: 224 1201
SphI G_CATG'C
Cuts at: 0 872 1425
Size: 872 553
SspI AAT'ATT
Cuts at: 0 820 1425
Size: 820 605
StuI AGG'CCT
Cuts at: 0 1177 1425
Size: 1177 248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 408 1425
Size: 408 1017
TatI w'GTAC_w
Cuts at: 0 542 564 665 1425
Size: 542 22 101 760
Fragments arranged by size:
760 542 101 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 541 573 1425
Size: 541 32 852
Fragments arranged by size:
852 541 32
Enzymes that do cut:
AccI AcuI AfeI AflIII AlfI AlwNI ApoI BanII
BbsI BclI BfrBI BglII Bme1580I BpmI BpuEI BsaBI
BsaWI BseRI BseYI BsiHKAI Bsp1286I BspEI BspHI BstYI
BstZ17I Bsu36I BtgZI DraI EaeI EarI EcoICRI Eco57MI
EcoO109I FspI HaeII Hin4I HincII MslI MspA1I NdeI
NsiI NspI PacI PciI PflMI PfoI PsiI PstI
PvuII SacI SfcI SmlI SphI SspI StuI TaqII
TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BglI
BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI
BsaHI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BtgI BtsI ClaI CspCI DraIII DrdI
EagI EciI EcoNI EcoRI EcoRV FalI FseI FspAI
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NcoI NgoMIV NheI NotI NruI PasI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI StyI SwaI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI