Restriction map for
pFN21AE4130
(Linear) (Six-base) MAPSORT of: KIEE4130.seq Check: 6044 from: 1 to: 1581
KIEE4130.orf
With 182 enzymes: SgfI *
March 22, 2012 17:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 833 1581
Size: 833 748
AccI GT'mk_AC
Cuts at: 0 999 1581
Size: 999 582
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 637 1114 1304 1363 1581
Size: 467 170 477 190 59 218
Fragments arranged by size:
477 467 218 190 170 59
AhdI GACnn_n'nnGTC
Cuts at: 0 1527 1581
Size: 1527 54
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 830 862 1581
Size: 830 32 719
Fragments arranged by size:
830 719 32
ApaLI G'TGCA_C
Cuts at: 0 1277 1581
Size: 1277 304
ApoI r'AATT_y
Cuts at: 0 225 574 863 1228 1581
Size: 225 349 289 365 353
Fragments arranged by size:
365 353 349 289 225
AseI AT'TA_AT
Cuts at: 0 790 844 1581
Size: 790 54 737
Fragments arranged by size:
790 737 54
AvaI C'yCGr_G
Cuts at: 0 130 1123 1581
Size: 130 993 458
Fragments arranged by size:
993 458 130
BamHI G'GATC_C
Cuts at: 0 199 1200 1581
Size: 199 1001 381
Fragments arranged by size:
1001 381 199
BanI G'GyrC_C
Cuts at: 0 185 680 1581
Size: 185 495 901
Fragments arranged by size:
901 495 185
BanII G_rGCy'C
Cuts at: 0 1402 1581
Size: 1402 179
BbsI GAAGACnn'nnnn_
Cuts at: 0 1102 1432 1581
Size: 1102 330 149
Fragments arranged by size:
1102 330 149
BbvCI CC'TCA_GC
Cuts at: 0 656 1581
Size: 656 925
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1488 1522 1581
Size: 1488 34 59
Fragments arranged by size:
1488 59 34
BclI T'GATC_A
Cuts at: 0 525 1368 1581
Size: 525 843 213
Fragments arranged by size:
843 525 213
BglII A'GATC_T
Cuts at: 0 771 1581
Size: 771 810
BlpI GC'TnA_GC
Cuts at: 0 376 1142 1581
Size: 376 766 439
Fragments arranged by size:
766 439 376
Bme1580I G_kGCm'C
Cuts at: 0 685 1281 1581
Size: 685 596 300
Fragments arranged by size:
685 596 300
BmgBI CAC'GTC
Cuts at: 0 1438 1581
Size: 1438 143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 736 1402 1581
Size: 712 24 666 179
Fragments arranged by size:
712 666 179 24
Bpu10I CC'TnA_GC
Cuts at: 0 656 1581
Size: 656 925
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 1581
Size: 727 854
BsaBI GATnn'nnATC
Cuts at: 0 800 1581
Size: 800 781
BsaWI w'CCGG_w
Cuts at: 0 583 882 1203 1581
Size: 583 299 321 378
Fragments arranged by size:
583 378 321 299
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 452 1141 1581
Size: 452 689 440
Fragments arranged by size:
689 452 440
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 1581
Size: 431 1150
BsiHKAI G_wGCw'C
Cuts at: 0 1281 1581
Size: 1281 300
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1048 1581
Size: 1048 533
Bsp1286I G_dGCh'C
Cuts at: 0 685 1281 1402 1581
Size: 685 596 121 179
Fragments arranged by size:
685 596 179 121
BspEI T'CCGG_A
Cuts at: 0 882 1203 1581
Size: 882 321 378
Fragments arranged by size:
882 378 321
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 833 1581
Size: 833 748
BsrDI GCAATG_nn'
Cuts at: 0 518 1581
Size: 518 1063
BstYI r'GATC_y
Cuts at: 0 199 771 1200 1581
Size: 199 572 429 381
Fragments arranged by size:
572 429 381 199
BtgI C'CryG_G
Cuts at: 0 92 1581
Size: 92 1489
DraI TTT'AAA
Cuts at: 0 177 1581
Size: 177 1404
EaeI y'GGCC_r
Cuts at: 0 1086 1581
Size: 1086 495
EarI CTCTTCn'nnn_
Cuts at: 0 30 1581
Size: 30 1551
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1149 1581
Size: 1149 432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 637 712 736 1114 1304 1363 1402
Size: 467 170 75 24 378 190 59 39
Cuts at: 1402 1581
Size: 179
Fragments arranged by size:
467 378 190 179 170 75 59 39
24
EcoO109I rG'GnC_Cy
Cuts at: 0 100 1581
Size: 100 1481
EcoRI G'AATT_C
Cuts at: 0 1228 1581
Size: 1228 353
EcoRV GAT'ATC
Cuts at: 0 956 1581
Size: 956 625
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 763 795 1513 1545 1581
Size: 763 32 718 32 36
Fragments arranged by size:
763 718 36 32 32
HindIII A'AGCT_T
Cuts at: 0 1160 1581
Size: 1160 421
MfeI C'AATT_G
Cuts at: 0 314 1581
Size: 314 1267
MscI TGG'CCA
Cuts at: 0 1088 1581
Size: 1088 493
MspA1I CmG'CkG
Cuts at: 0 19 375 807 1092 1581
Size: 19 356 432 285 489
Fragments arranged by size:
489 432 356 285 19
NspI r_CATG'y
Cuts at: 0 61 455 1581
Size: 61 394 1126
Fragments arranged by size:
1126 394 61
PfoI T'CCnGG_A
Cuts at: 0 1379 1581
Size: 1379 202
PpuMI rG'GwC_Cy
Cuts at: 0 100 1581
Size: 100 1481
PvuII CAG'CTG
Cuts at: 0 375 807 1092 1581
Size: 375 432 285 489
Fragments arranged by size:
489 432 375 285
SexAI A'CCwGG_T
Cuts at: 0 932 1581
Size: 932 649
SmlI C'TyrA_G
Cuts at: 0 706 1581
Size: 706 875
StuI AGG'CCT
Cuts at: 0 689 1581
Size: 689 892
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 887 1581
Size: 887 694
TatI w'GTAC_w
Cuts at: 0 612 1581
Size: 612 969
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1227 1259 1581
Size: 1227 32 322
Fragments arranged by size:
1227 322 32
XmnI GAAnn'nnTTC
Cuts at: 0 606 867 1581
Size: 606 261 714
Fragments arranged by size:
714 606 261
Enzymes that do cut:
AarI AccI AcuI AhdI AloI ApaLI ApoI AseI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BclI
BglII BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaBI
BsaWI BseRI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI
BsrDI BstYI BtgI DraI EaeI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV Hin4I HindIII MfeI MscI MspA1I
NspI PfoI PpuMI PvuII SexAI SmlI StuI TaqII
TatI TstI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AlwNI ApaI AscI AsiSI AvrII BaeI BbeI
BciVI BfrBI BglI BmrI BmtI BplI BsaI BsaAI
BsaHI BsaXI BseYI BsiEI BsiWI BsmI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI
EagI EcoICRI EcoNI FalI FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MluI MmeI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StyI SwaI Tth111I XbaI XcmI XhoI XmaI
ZraI