Restriction map for
pFN21AE4129
(Linear) (Six-base) MAPSORT of: KIEE4129.seq Check: 8452 from: 1 to: 1578
KIEE4129.orf
With 182 enzymes: SgfI *
March 22, 2012 17:25 ..
AccI GT'mk_AC
Cuts at: 0 275 1578
Size: 275 1303
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 172 1179 1523 1534 1578
Size: 91 81 1007 344 11 44
Fragments arranged by size:
1007 344 91 81 44 11
AflII C'TTAA_G
Cuts at: 0 838 1578
Size: 838 740
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 335 367 1578
Size: 335 32 1211
Fragments arranged by size:
1211 335 32
ApaLI G'TGCA_C
Cuts at: 0 336 1578
Size: 336 1242
ApoI r'AATT_y
Cuts at: 0 154 311 1424 1578
Size: 154 157 1113 154
Fragments arranged by size:
1113 157 154 154
AseI AT'TA_AT
Cuts at: 0 484 488 1578
Size: 484 4 1090
Fragments arranged by size:
1090 484 4
AvaI C'yCGr_G
Cuts at: 0 971 1578
Size: 971 607
AvrII C'CTAG_G
Cuts at: 0 1404 1578
Size: 1404 174
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 968 1001 1578
Size: 968 33 577
Fragments arranged by size:
968 577 33
BanII G_rGCy'C
Cuts at: 0 64 786 1314 1578
Size: 64 722 528 264
Fragments arranged by size:
722 528 264 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 97 1578
Size: 97 1481
BciVI GTATCCnnnnn_n'
Cuts at: 0 361 969 1354 1402 1578
Size: 361 608 385 48 176
Fragments arranged by size:
608 385 361 176 48
Bme1580I G_kGCm'C
Cuts at: 0 340 1578
Size: 340 1238
BmrI ACTGGGnnnn_n'
Cuts at: 0 640 1578
Size: 640 938
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 496 1578
Size: 40 456 1082
Fragments arranged by size:
1082 456 40
BsaI GGTCTCn'nnnn_
Cuts at: 0 105 1578
Size: 105 1473
BsaAI yAC'GTr
Cuts at: 0 688 1578
Size: 688 890
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 1578
Size: 25 1553
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 1578
Size: 227 1351
BsiHKAI G_wGCw'C
Cuts at: 0 340 1578
Size: 340 1238
BsmI GAATG_Cn'
Cuts at: 0 198 601 1000 1578
Size: 198 403 399 578
Fragments arranged by size:
578 403 399 198
Bsp1286I G_dGCh'C
Cuts at: 0 64 340 786 1314 1578
Size: 64 276 446 528 264
Fragments arranged by size:
528 446 276 264 64
BsrDI GCAATG_nn'
Cuts at: 0 918 1578
Size: 918 660
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1513 1578
Size: 1513 65
BstZ17I GTA'TAC
Cuts at: 0 276 1578
Size: 276 1302
Bsu36I CC'TnA_GG
Cuts at: 0 464 812 1578
Size: 464 348 766
Fragments arranged by size:
766 464 348
BtgI C'CryG_G
Cuts at: 0 758 1578
Size: 758 820
BtsI GCAGTG_nn'
Cuts at: 0 1472 1578
Size: 1472 106
DraI TTT'AAA
Cuts at: 0 162 326 1532 1578
Size: 162 164 1206 46
Fragments arranged by size:
1206 164 162 46
DrdI GACnn_nn'nnGTC
Cuts at: 0 752 1578
Size: 752 826
EarI CTCTTCn'nnn_
Cuts at: 0 313 1218 1265 1550 1578
Size: 313 905 47 285 28
Fragments arranged by size:
905 313 285 47 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 172 1179 1523 1534 1578
Size: 91 81 1007 344 11 44
Fragments arranged by size:
1007 344 91 81 44 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1477 1578
Size: 1477 101
EcoRI G'AATT_C
Cuts at: 0 154 1578
Size: 154 1424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 174 412 1528 1578
Size: 174 238 1116 50
Fragments arranged by size:
1116 238 174 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1471 1503 1578
Size: 1471 32 75
Fragments arranged by size:
1471 75 32
HincII GTy'rAC
Cuts at: 0 902 1578
Size: 902 676
MslI CAynn'nnrTG
Cuts at: 0 1335 1578
Size: 1335 243
MspA1I CmG'CkG
Cuts at: 0 81 1444 1501 1578
Size: 81 1363 57 77
Fragments arranged by size:
1363 81 77 57
NspI r_CATG'y
Cuts at: 0 685 1578
Size: 685 893
PacI TTA_AT'TAA
Cuts at: 0 488 1578
Size: 488 1090
PflMI CCAn_nnn'nTGG
Cuts at: 0 1081 1578
Size: 1081 497
PstI C_TGCA'G
Cuts at: 0 1500 1578
Size: 1500 78
PvuII CAG'CTG
Cuts at: 0 81 1444 1501 1578
Size: 81 1363 57 77
Fragments arranged by size:
1363 81 77 57
SfcI C'TryA_G
Cuts at: 0 1370 1496 1578
Size: 1370 126 82
Fragments arranged by size:
1370 126 82
SmaI CCC'GGG
Cuts at: 0 973 1578
Size: 973 605
SmlI C'TyrA_G
Cuts at: 0 55 475 838 1578
Size: 55 420 363 740
Fragments arranged by size:
740 420 363 55
SnaBI TAC'GTA
Cuts at: 0 688 1578
Size: 688 890
SspI AAT'ATT
Cuts at: 0 798 1578
Size: 798 780
StuI AGG'CCT
Cuts at: 0 94 1578
Size: 94 1484
StyI C'CwwG_G
Cuts at: 0 1404 1578
Size: 1404 174
TatI w'GTAC_w
Cuts at: 0 71 737 1578
Size: 71 666 841
Fragments arranged by size:
841 666 71
XmaI C'CCGG_G
Cuts at: 0 971 1578
Size: 971 607
XmnI GAAnn'nnTTC
Cuts at: 0 1259 1508 1578
Size: 1259 249 70
Fragments arranged by size:
1259 249 70
Enzymes that do cut:
AccI AcuI AflII AloI ApaLI ApoI AseI AvaI
AvrII BaeI BanII BbsI BciVI Bme1580I BmrI BpuEI
BsaI BsaAI BseRI BsgI BsiHKAI BsmI Bsp1286I BsrDI
BstAPI BstZ17I Bsu36I BtgI BtsI DraI DrdI EarI
Eco57MI EcoNI EcoRI FalI Hin4I HincII MslI MspA1I
NspI PacI PflMI PstI PvuII SfcI SmaI SmlI
SnaBI SspI StuI StyI TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI AscI AsiSI BamHI BanI
BbeI BbvCI BcgI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI BpmI Bpu10I BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI
BstYI BtgZI ClaI CspCI DraIII EaeI EagI EciI
EcoICRI EcoO109I EcoRV FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SpeI SphI SrfI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI ZraI