Restriction map for
pFN21AE4126
(Linear) (Six-base) MAPSORT of: KIEE4126.seq Check: 3250 from: 1 to: 1551
KIEE4126.orf
With 182 enzymes: SgfI *
March 22, 2012 17:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 708 1044 1104 1551
Size: 708 336 60 447
Fragments arranged by size:
708 447 336 60
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 1551
Size: 847 704
AgeI A'CCGG_T
Cuts at: 0 939 1551
Size: 939 612
AlwNI CAG_nnn'CTG
Cuts at: 0 15 570 627 657 1419 1513 1551
Size: 15 555 57 30 762 94 38
Fragments arranged by size:
762 555 94 57 38 30 15
ApaI G_GGCC'C
Cuts at: 0 230 1084 1094 1232 1532 1551
Size: 230 854 10 138 300 19
Fragments arranged by size:
854 300 230 138 19 10
ApaLI G'TGCA_C
Cuts at: 0 348 1551
Size: 348 1203
AvaI C'yCGr_G
Cuts at: 0 142 233 638 1076 1474 1551
Size: 142 91 405 438 398 77
Fragments arranged by size:
438 405 398 142 91 77
BamHI G'GATC_C
Cuts at: 0 237 698 1551
Size: 237 461 853
Fragments arranged by size:
853 461 237
BanI G'GyrC_C
Cuts at: 0 169 885 936 1145 1551
Size: 169 716 51 209 406
Fragments arranged by size:
716 406 209 169 51
BanII G_rGCy'C
Cuts at: 0 53 158 230 311 710 1084 1094 1109
Size: 53 105 72 81 399 374 10 15
Cuts at: 1109 1232 1274 1406 1532 1551
Size: 123 42 132 126 19
Fragments arranged by size:
399 374 132 126 123 105 81 72
53 42 19 15 10
BbeI G_GCGC'C
Cuts at: 0 173 1551
Size: 173 1378
BbsI GAAGACnn'nnnn_
Cuts at: 0 812 1455 1551
Size: 812 643 96
Fragments arranged by size:
812 643 96
BbvCI CC'TCA_GC
Cuts at: 0 196 1551
Size: 196 1355
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 970 1004 1551
Size: 970 34 547
Fragments arranged by size:
970 547 34
BglI GCCn_nnn'nGGC
Cuts at: 0 460 768 777 1551
Size: 460 308 9 774
Fragments arranged by size:
774 460 308 9
BlpI GC'TnA_GC
Cuts at: 0 838 1546 1551
Size: 838 708 5
Fragments arranged by size:
838 708 5
Bme1580I G_kGCm'C
Cuts at: 0 142 230 352 888 1084 1094 1128 1148
Size: 142 88 122 536 196 10 34 20
Cuts at: 1148 1232 1532 1551
Size: 84 300 19
Fragments arranged by size:
536 300 196 142 122 88 84 34
20 19 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 58 1164 1198 1551
Size: 58 1106 34 353
Fragments arranged by size:
1106 353 58 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 229 325 454 733 742 1279 1420
Size: 37 192 96 129 279 9 537 141
Cuts at: 1420 1551
Size: 131
Fragments arranged by size:
537 279 192 141 131 129 96 37
9
Bpu10I CC'TnA_GC
Cuts at: 0 196 1551
Size: 196 1355
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 1551
Size: 388 1163
BsaI GGTCTCn'nnnn_
Cuts at: 0 543 1551
Size: 543 1008
BsaHI Gr'CG_yC
Cuts at: 0 170 1193 1551
Size: 170 1023 358
Fragments arranged by size:
1023 358 170
BsaWI w'CCGG_w
Cuts at: 0 939 1551
Size: 939 612
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 612 661 745 847 981 1462 1551
Size: 34 578 49 84 102 134 481 89
Fragments arranged by size:
578 481 134 102 89 84 49 34
BseYI C'CCAG_C
Cuts at: 0 416 443 816 1224 1319 1412 1551
Size: 416 27 373 408 95 93 139
Fragments arranged by size:
416 408 373 139 95 93 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 1239 1551
Size: 360 879 312
Fragments arranged by size:
879 360 312
BsiEI CG_ry'CG
Cuts at: 0 301 1551
Size: 301 1250
BsiHKAI G_wGCw'C
Cuts at: 0 311 352 710 1109 1547 1551
Size: 311 41 358 399 438 4
Fragments arranged by size:
438 399 358 311 41 4
BsmBI CGTCTCn'nnnn_
Cuts at: 0 635 1200 1551
Size: 635 565 351
Fragments arranged by size:
635 565 351
Bsp1286I G_dGCh'C
Cuts at: 0 53 142 158 230 311 352 710 888
Size: 53 89 16 72 81 41 358 178
Cuts at: 888 1084 1094 1109 1128 1148 1232 1274 1406
Size: 196 10 15 19 20 84 42 132
Cuts at: 1406 1532 1547 1551
Size: 126 15 4
Fragments arranged by size:
358 196 178 132 126 89 84 81
72 53 42 41 20 19 16 15
15 10 4
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 40 708 1044 1104 1551
Size: 40 668 336 60 447
Fragments arranged by size:
668 447 336 60 40
BsrBI CCG'CTC
Cuts at: 0 378 1551
Size: 378 1173
BsrDI GCAATG_nn'
Cuts at: 0 1126 1551
Size: 1126 425
BsrFI r'CCGG_y
Cuts at: 0 842 939 1551
Size: 842 97 612
Fragments arranged by size:
842 612 97
BsrGI T'GTAC_A
Cuts at: 0 1421 1551
Size: 1421 130
BssHII G'CGCG_C
Cuts at: 0 219 277 1164 1551
Size: 219 58 887 387
Fragments arranged by size:
887 387 219 58
BssSI C'ACGA_G
Cuts at: 0 373 1551
Size: 373 1178
BstAPI GCAn_nnn'nTGC
Cuts at: 0 347 657 1551
Size: 347 310 894
Fragments arranged by size:
894 347 310
BstEII G'GTnAC_C
Cuts at: 0 438 820 1551
Size: 438 382 731
Fragments arranged by size:
731 438 382
BstXI CCAn_nnnn'nTGG
Cuts at: 0 45 900 1551
Size: 45 855 651
Fragments arranged by size:
855 651 45
BstYI r'GATC_y
Cuts at: 0 237 503 698 1551
Size: 237 266 195 853
Fragments arranged by size:
853 266 237 195
Bsu36I CC'TnA_GG
Cuts at: 0 1374 1551
Size: 1374 177
BtgI C'CryG_G
Cuts at: 0 291 687 914 1213 1551
Size: 291 396 227 299 338
Fragments arranged by size:
396 338 299 291 227
BtsI GCAGTG_nn'
Cuts at: 0 802 1551
Size: 802 749
DraIII CAC_nnn'GTG
Cuts at: 0 1182 1551
Size: 1182 369
EaeI y'GGCC_r
Cuts at: 0 2 81 298 611 1248 1551
Size: 2 79 217 313 637 303
Fragments arranged by size:
637 313 303 217 79 2
EagI C'GGCC_G
Cuts at: 0 298 1551
Size: 298 1253
EarI CTCTTCn'nnn_
Cuts at: 0 316 860 1444 1532 1551
Size: 316 544 584 88 19
Fragments arranged by size:
584 544 316 88 19
EcoICRI GAG'CTC
Cuts at: 0 309 708 1107 1551
Size: 309 399 399 444
Fragments arranged by size:
444 399 399 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 229 325 454 733 742 847 1279
Size: 37 192 96 129 279 9 105 432
Cuts at: 1279 1420 1551
Size: 141 131
Fragments arranged by size:
432 279 192 141 131 129 105 96
37 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 562 715 1551
Size: 562 153 836
Fragments arranged by size:
836 562 153
EcoO109I rG'GnC_Cy
Cuts at: 0 60 98 567 769 921 928 1080 1228
Size: 60 38 469 202 152 7 152 148
Cuts at: 1228 1229 1255 1484 1528 1529 1551
Size: 1 26 229 44 1 22
Fragments arranged by size:
469 229 202 152 152 148 60 44
38 26 22 7 1 1
FspI TGC'GCA
Cuts at: 0 487 1551
Size: 487 1064
HaeII r_GCGC'y
Cuts at: 0 173 385 402 628 1019 1326 1551
Size: 173 212 17 226 391 307 225
Fragments arranged by size:
391 307 226 225 212 173 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 990 1022 1357 1389 1551
Size: 990 32 335 32 162
Fragments arranged by size:
990 335 162 32 32
KasI G'GCGC_C
Cuts at: 0 169 1551
Size: 169 1382
MscI TGG'CCA
Cuts at: 0 613 1551
Size: 613 938
MslI CAynn'nnrTG
Cuts at: 0 607 1551
Size: 607 944
MspA1I CmG'CkG
Cuts at: 0 12 125 200 303 332 420 654 816
Size: 12 113 75 103 29 88 234 162
Cuts at: 816 1551
Size: 735
Fragments arranged by size:
735 234 162 113 103 88 75 29
12
NaeI GCC'GGC
Cuts at: 0 844 1551
Size: 844 707
NarI GG'CG_CC
Cuts at: 0 170 1551
Size: 170 1381
NcoI C'CATG_G
Cuts at: 0 687 1551
Size: 687 864
NgoMIV G'CCGG_C
Cuts at: 0 842 1551
Size: 842 709
NotI GC'GGCC_GC
Cuts at: 0 298 1551
Size: 298 1253
PasI CC'CwG_GG
Cuts at: 0 766 880 1330 1551
Size: 766 114 450 221
Fragments arranged by size:
766 450 221 114
PflMI CCAn_nnn'nTGG
Cuts at: 0 816 1551
Size: 816 735
PfoI T'CCnGG_A
Cuts at: 0 107 701 710 1280 1551
Size: 107 594 9 570 271
Fragments arranged by size:
594 570 271 107 9
PpuMI rG'GwC_Cy
Cuts at: 0 60 928 1255 1484 1551
Size: 60 868 327 229 67
Fragments arranged by size:
868 327 229 67 60
PspOMI G'GGCC_C
Cuts at: 0 226 1080 1090 1228 1528 1551
Size: 226 854 10 138 300 23
Fragments arranged by size:
854 300 226 138 23 10
PspXI vC'TCGA_Gb
Cuts at: 0 1474 1551
Size: 1474 77
PstI C_TGCA'G
Cuts at: 0 697 743 797 1551
Size: 697 46 54 754
Fragments arranged by size:
754 697 54 46
PvuII CAG'CTG
Cuts at: 0 12 420 816 1551
Size: 12 408 396 735
Fragments arranged by size:
735 408 396 12
RsrII CG'GwC_CG
Cuts at: 0 320 1551
Size: 320 1231
SacI G_AGCT'C
Cuts at: 0 311 710 1109 1551
Size: 311 399 399 442
Fragments arranged by size:
442 399 399 311
SanDI GG'GwC_CC
Cuts at: 0 1255 1551
Size: 1255 296
SapI GCTCTTCn'nnn_
Cuts at: 0 316 1551
Size: 316 1235
SbfI CC_TGCA'GG
Cuts at: 0 743 1551
Size: 743 808
SfcI C'TryA_G
Cuts at: 0 562 693 739 793 1392 1551
Size: 562 131 46 54 599 159
Fragments arranged by size:
599 562 159 131 54 46
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 460 777 1551
Size: 460 317 774
Fragments arranged by size:
774 460 317
SfoI GGC'GCC
Cuts at: 0 171 1551
Size: 171 1380
SmaI CCC'GGG
Cuts at: 0 235 1078 1551
Size: 235 843 473
Fragments arranged by size:
843 473 235
SmlI C'TyrA_G
Cuts at: 0 403 638 1474 1551
Size: 403 235 836 77
Fragments arranged by size:
836 403 235 77
StuI AGG'CCT
Cuts at: 0 1300 1551
Size: 1300 251
StyI C'CwwG_G
Cuts at: 0 687 1551
Size: 687 864
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 337 472 808 1551
Size: 337 135 336 743
Fragments arranged by size:
743 337 336 135
TatI w'GTAC_w
Cuts at: 0 1421 1551
Size: 1421 130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 942 974 1551
Size: 942 32 577
Fragments arranged by size:
942 577 32
XhoI C'TCGA_G
Cuts at: 0 638 1474 1551
Size: 638 836 77
Fragments arranged by size:
836 638 77
XmaI C'CCGG_G
Cuts at: 0 233 1076 1551
Size: 233 843 475
Fragments arranged by size:
843 475 233
Enzymes that do cut:
AarI AcuI AgeI AlwNI ApaI ApaLI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI PasI PflMI PfoI PpuMI PspOMI PspXI PstI
PvuII RsrII SacI SanDI SapI SbfI SfcI SfiI
SfoI SmaI SmlI StuI StyI TaqII TatI TstI
XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BciVI BclI BfrBI BglII BmgBI BmtI BplI
BsaAI BsaBI BsaXI BsiWI BsmI BspEI BspHI BstBI
BstZ17I BtgZI ClaI CspCI DraI DrdI EciI EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI NdeI NheI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PshAI PsiI PsrI
PvuI SacII SalI ScaI SexAI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI
XmnI ZraI