Restriction map for
pFN21AE4121
(Linear) (Six-base) MAPSORT of: KIEE4121.seq Check: 7603 from: 1 to: 1515
KIEE4121.orf
With 182 enzymes: SgfI *
March 22, 2012 17:24 ..
AclI AA'CG_TT
Cuts at: 0 1463 1515
Size: 1463 52
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 1018 1515
Size: 339 679 497
Fragments arranged by size:
679 497 339
AflII C'TTAA_G
Cuts at: 0 49 1515
Size: 49 1466
AflIII A'CryG_T
Cuts at: 0 1193 1515
Size: 1193 322
AhdI GACnn_n'nnGTC
Cuts at: 0 1155 1515
Size: 1155 360
AlwNI CAG_nnn'CTG
Cuts at: 0 798 1515
Size: 798 717
ApaI G_GGCC'C
Cuts at: 0 276 1515
Size: 276 1239
ApoI r'AATT_y
Cuts at: 0 68 349 389 472 1515
Size: 68 281 40 83 1043
Fragments arranged by size:
1043 281 83 68 40
AvaI C'yCGr_G
Cuts at: 0 893 1515
Size: 893 622
BanII G_rGCy'C
Cuts at: 0 276 774 1515
Size: 276 498 741
Fragments arranged by size:
741 498 276
BbsI GAAGACnn'nnnn_
Cuts at: 0 2 328 824 1515
Size: 2 326 496 691
Fragments arranged by size:
691 496 326 2
BfrBI ATG'CAT
Cuts at: 0 543 1515
Size: 543 972
BglII A'GATC_T
Cuts at: 0 744 1515
Size: 744 771
Bme1580I G_kGCm'C
Cuts at: 0 276 1515
Size: 276 1239
BmrI ACTGGGnnnn_n'
Cuts at: 0 213 404 1149 1515
Size: 213 191 745 366
Fragments arranged by size:
745 366 213 191
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1256 1515
Size: 1256 259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 208 626 1109 1515
Size: 177 31 418 483 406
Fragments arranged by size:
483 418 406 177 31
Bpu10I CC'TnA_GC
Cuts at: 0 587 1254 1515
Size: 587 667 261
Fragments arranged by size:
667 587 261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 1484 1515
Size: 463 1021 31
Fragments arranged by size:
1021 463 31
BsaI GGTCTCn'nnnn_
Cuts at: 0 1244 1515
Size: 1244 271
BsaBI GATnn'nnATC
Cuts at: 0 1184 1515
Size: 1184 331
BsaHI Gr'CG_yC
Cuts at: 0 7 970 1151 1259 1515
Size: 7 963 181 108 256
Fragments arranged by size:
963 256 181 108 7
BseYI C'CCAG_C
Cuts at: 0 779 1515
Size: 779 736
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 942 1515
Size: 942 573
BsiHKAI G_wGCw'C
Cuts at: 0 588 1515
Size: 588 927
BsmI GAATG_Cn'
Cuts at: 0 545 1515
Size: 545 970
BsmBI CGTCTCn'nnnn_
Cuts at: 0 700 1515
Size: 700 815
Bsp1286I G_dGCh'C
Cuts at: 0 276 588 774 1515
Size: 276 312 186 741
Fragments arranged by size:
741 312 276 186
BspHI T'CATG_A
Cuts at: 0 686 1322 1515
Size: 686 636 193
Fragments arranged by size:
686 636 193
BsrFI r'CCGG_y
Cuts at: 0 425 966 1515
Size: 425 541 549
Fragments arranged by size:
549 541 425
BssSI C'ACGA_G
Cuts at: 0 626 1515
Size: 626 889
BstEII G'GTnAC_C
Cuts at: 0 333 1515
Size: 333 1182
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1341 1515
Size: 1341 174
BstYI r'GATC_y
Cuts at: 0 87 555 744 1284 1515
Size: 87 468 189 540 231
Fragments arranged by size:
540 468 231 189 87
BtsI GCAGTG_nn'
Cuts at: 0 1489 1515
Size: 1489 26
DraIII CAC_nnn'GTG
Cuts at: 0 606 920 1515
Size: 606 314 595
Fragments arranged by size:
606 595 314
EaeI y'GGCC_r
Cuts at: 0 608 1200 1515
Size: 608 592 315
Fragments arranged by size:
608 592 315
EarI CTCTTCn'nnn_
Cuts at: 0 158 233 1515
Size: 158 75 1282
Fragments arranged by size:
1282 158 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 229 1515
Size: 229 1286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 208 339 626 1018 1109 1515
Size: 177 31 131 287 392 91 406
Fragments arranged by size:
406 392 287 177 131 91 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1212 1515
Size: 1212 303
EcoO109I rG'GnC_Cy
Cuts at: 0 272 1515
Size: 272 1243
EcoRI G'AATT_C
Cuts at: 0 68 1515
Size: 68 1447
EcoRV GAT'ATC
Cuts at: 0 864 1515
Size: 864 651
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 369 951 1515
Size: 369 582 564
Fragments arranged by size:
582 564 369
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 439 471 609 641 651 683 955 987
Size: 439 32 138 32 10 32 272 32
Cuts at: 987 1141 1173 1222 1254 1276 1308 1515
Size: 154 32 49 32 22 32 207
Fragments arranged by size:
439 272 207 154 138 49 32 32
32 32 32 32 32 22 10
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 1515
Size: 354 1161
MscI TGG'CCA
Cuts at: 0 1202 1515
Size: 1202 313
MslI CAynn'nnrTG
Cuts at: 0 871 1515
Size: 871 644
MspA1I CmG'CkG
Cuts at: 0 228 976 1515
Size: 228 748 539
Fragments arranged by size:
748 539 228
NaeI GCC'GGC
Cuts at: 0 968 1515
Size: 968 547
NgoMIV G'CCGG_C
Cuts at: 0 966 1515
Size: 966 549
NsiI A_TGCA'T
Cuts at: 0 545 1515
Size: 545 970
NspI r_CATG'y
Cuts at: 0 1197 1515
Size: 1197 318
PciI A'CATG_T
Cuts at: 0 1193 1515
Size: 1193 322
PflMI CCAn_nnn'nTGG
Cuts at: 0 24 155 606 1515
Size: 24 131 451 909
Fragments arranged by size:
909 451 131 24
PspOMI G'GGCC_C
Cuts at: 0 272 1515
Size: 272 1243
SmlI C'TyrA_G
Cuts at: 0 49 478 893 1499 1515
Size: 49 429 415 606 16
Fragments arranged by size:
606 429 415 49 16
StuI AGG'CCT
Cuts at: 0 244 1515
Size: 244 1271
StyI C'CwwG_G
Cuts at: 0 267 1515
Size: 267 1248
TatI w'GTAC_w
Cuts at: 0 680 1515
Size: 680 835
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 59 79 91 111 1515
Size: 59 20 12 20 1404
Fragments arranged by size:
1404 59 20 20 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 528 1515
Size: 528 987
XhoI C'TCGA_G
Cuts at: 0 893 1515
Size: 893 622
XmnI GAAnn'nnTTC
Cuts at: 0 349 352 1515
Size: 349 3 1163
Fragments arranged by size:
1163 349 3
Enzymes that do cut:
AclI AcuI AflII AflIII AhdI AlwNI ApaI ApoI
AvaI BanII BbsI BfrBI BglII Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrFI BssSI BstEII
BstXI BstYI BtsI DraIII EaeI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI Hin4I MmeI MscI
MslI MspA1I NaeI NgoMIV NsiI NspI PciI PflMI
PspOMI SmlI StuI StyI TatI TstI XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AgeI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BciVI BclI BglI BlpI
BmgBI BmtI BsaAI BsaWI BsaXI BseRI BsiEI BsiWI
BspEI BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstBI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI
EagI EcoICRI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI NarI NcoI NdeI
NheI NotI NruI PacI PasI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I
XbaI XmaI ZraI