Restriction map for
pFN21AE4119
(Linear) (Six-base) MAPSORT of: KIEE4119.seq Check: 5598 from: 1 to: 1422
KIEE4119.orf
With 182 enzymes: SgfI *
July 3, 2012 18:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1331 1422
Size: 1331 91
AatII G_ACGT'C
Cuts at: 0 1124 1422
Size: 1124 298
AclI AA'CG_TT
Cuts at: 0 1040 1422
Size: 1040 382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 700 878 932 1422
Size: 559 141 178 54 490
Fragments arranged by size:
559 490 178 141 54
AfeI AGC'GCT
Cuts at: 0 1305 1422
Size: 1305 117
AflII C'TTAA_G
Cuts at: 0 403 1422
Size: 403 1019
AflIII A'CryG_T
Cuts at: 0 626 1422
Size: 626 796
AhdI GACnn_n'nnGTC
Cuts at: 0 66 1422
Size: 66 1356
AlwNI CAG_nnn'CTG
Cuts at: 0 1411 1422
Size: 1411 11
ApaI G_GGCC'C
Cuts at: 0 198 241 1422
Size: 198 43 1181
Fragments arranged by size:
1181 198 43
ApaLI G'TGCA_C
Cuts at: 0 1318 1422
Size: 1318 104
AvaI C'yCGr_G
Cuts at: 0 249 1422
Size: 249 1173
BanI G'GyrC_C
Cuts at: 0 120 158 168 230 360 1422
Size: 120 38 10 62 130 1062
Fragments arranged by size:
1062 130 120 62 38 10
BanII G_rGCy'C
Cuts at: 0 198 241 1422
Size: 198 43 1181
Fragments arranged by size:
1181 198 43
BbeI G_GCGC'C
Cuts at: 0 124 172 234 1422
Size: 124 48 62 1188
Fragments arranged by size:
1188 124 62 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 223 796 866 876 994 1422
Size: 223 573 70 10 118 428
Fragments arranged by size:
573 428 223 118 70 10
BbvCI CC'TCA_GC
Cuts at: 0 8 1422
Size: 8 1414
BfrBI ATG'CAT
Cuts at: 0 447 1422
Size: 447 975
BglI GCCn_nnn'nGGC
Cuts at: 0 260 282 1422
Size: 260 22 1140
Fragments arranged by size:
1140 260 22
Bme1580I G_kGCm'C
Cuts at: 0 198 241 248 1322 1422
Size: 198 43 7 1074 100
Fragments arranged by size:
1074 198 100 43 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 126 201 279 1074 1292 1422
Size: 126 75 78 795 218 130
Fragments arranged by size:
795 218 130 126 78 75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 713 998 1422
Size: 713 285 424
Fragments arranged by size:
713 424 285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 1111 1422
Size: 727 384 311
Fragments arranged by size:
727 384 311
Bpu10I CC'TnA_GC
Cuts at: 0 8 1422
Size: 8 1414
BsaAI yAC'GTr
Cuts at: 0 906 1422
Size: 906 516
BsaHI Gr'CG_yC
Cuts at: 0 121 169 231 1121 1422
Size: 121 48 62 890 301
Fragments arranged by size:
890 301 121 62 48
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 1422
Size: 37 1385
BseYI C'CCAG_C
Cuts at: 0 1232 1422
Size: 1232 190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 345 523 1309 1422
Size: 91 254 178 786 113
Fragments arranged by size:
786 254 178 113 91
BsiEI CG_ry'CG
Cuts at: 0 264 1422
Size: 264 1158
BsiHKAI G_wGCw'C
Cuts at: 0 344 1322 1422
Size: 344 978 100
Fragments arranged by size:
978 344 100
BsmBI CGTCTCn'nnnn_
Cuts at: 0 595 1422
Size: 595 827
Bsp1286I G_dGCh'C
Cuts at: 0 198 241 248 344 1322 1422
Size: 198 43 7 96 978 100
Fragments arranged by size:
978 198 100 96 43 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 40 1331 1422
Size: 40 1291 91
Fragments arranged by size:
1291 91 40
BsrFI r'CCGG_y
Cuts at: 0 227 1422
Size: 227 1195
BssSI C'ACGA_G
Cuts at: 0 421 1422
Size: 421 1001
BstXI CCAn_nnnn'nTGG
Cuts at: 0 45 1370 1422
Size: 45 1325 52
Fragments arranged by size:
1325 52 45
BstYI r'GATC_y
Cuts at: 0 749 1422
Size: 749 673
Bsu36I CC'TnA_GG
Cuts at: 0 1050 1422
Size: 1050 372
BtgI C'CryG_G
Cuts at: 0 96 356 1422
Size: 96 260 1066
Fragments arranged by size:
1066 260 96
DraIII CAC_nnn'GTG
Cuts at: 0 482 1257 1422
Size: 482 775 165
Fragments arranged by size:
775 482 165
EaeI y'GGCC_r
Cuts at: 0 81 200 261 307 425 934 1360 1422
Size: 81 119 61 46 118 509 426 62
Fragments arranged by size:
509 426 119 118 81 62 61 46
EagI C'GGCC_G
Cuts at: 0 261 1422
Size: 261 1161
EarI CTCTTCn'nnn_
Cuts at: 0 292 554 593 682 1422
Size: 292 262 39 89 740
Fragments arranged by size:
740 292 262 89 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 700 727 878 932 1111 1422
Size: 559 141 27 151 54 179 311
Fragments arranged by size:
559 311 179 151 141 54 27
EcoO109I rG'GnC_Cy
Cuts at: 0 60 195 238 564 1422
Size: 60 135 43 326 858
Fragments arranged by size:
858 326 135 60 43
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 687 1422
Size: 687 735
HaeII r_GCGC'y
Cuts at: 0 124 172 228 234 1307 1422
Size: 124 48 56 6 1073 115
Fragments arranged by size:
1073 124 115 56 48 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 486 518 1422
Size: 52 32 402 32 904
Fragments arranged by size:
904 402 52 32 32
HincII GTy'rAC
Cuts at: 0 29 737 1422
Size: 29 708 685
Fragments arranged by size:
708 685 29
KasI G'GCGC_C
Cuts at: 0 120 168 230 1422
Size: 120 48 62 1192
Fragments arranged by size:
1192 120 62 48
MscI TGG'CCA
Cuts at: 0 202 427 936 1422
Size: 202 225 509 486
Fragments arranged by size:
509 486 225 202
MspA1I CmG'CkG
Cuts at: 0 98 279 306 1422
Size: 98 181 27 1116
Fragments arranged by size:
1116 181 98 27
NaeI GCC'GGC
Cuts at: 0 229 1422
Size: 229 1193
NarI GG'CG_CC
Cuts at: 0 121 169 231 1422
Size: 121 48 62 1191
Fragments arranged by size:
1191 121 62 48
NcoI C'CATG_G
Cuts at: 0 356 1422
Size: 356 1066
NgoMIV G'CCGG_C
Cuts at: 0 227 1422
Size: 227 1195
NotI GC'GGCC_GC
Cuts at: 0 261 1422
Size: 261 1161
NsiI A_TGCA'T
Cuts at: 0 449 1422
Size: 449 973
PflMI CCAn_nnn'nTGG
Cuts at: 0 482 932 1257 1422
Size: 482 450 325 165
Fragments arranged by size:
482 450 325 165
PfoI T'CCnGG_A
Cuts at: 0 17 56 752 1422
Size: 17 39 696 670
Fragments arranged by size:
696 670 39 17
PpuMI rG'GwC_Cy
Cuts at: 0 60 564 1422
Size: 60 504 858
Fragments arranged by size:
858 504 60
PspOMI G'GGCC_C
Cuts at: 0 194 237 1422
Size: 194 43 1185
Fragments arranged by size:
1185 194 43
SacII CC_GC'GG
Cuts at: 0 99 1422
Size: 99 1323
SapI GCTCTTCn'nnn_
Cuts at: 0 292 682 1422
Size: 292 390 740
Fragments arranged by size:
740 390 292
SfcI C'TryA_G
Cuts at: 0 1299 1422
Size: 1299 123
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 260 1422
Size: 260 1162
SfoI GGC'GCC
Cuts at: 0 122 170 232 1422
Size: 122 48 62 1190
Fragments arranged by size:
1190 122 62 48
SgrAI Cr'CCGG_yG
Cuts at: 0 227 1422
Size: 227 1195
SmaI CCC'GGG
Cuts at: 0 251 1422
Size: 251 1171
SmlI C'TyrA_G
Cuts at: 0 403 1422
Size: 403 1019
StuI AGG'CCT
Cuts at: 0 376 1422
Size: 376 1046
StyI C'CwwG_G
Cuts at: 0 356 1422
Size: 356 1066
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 176 378 1246 1422
Size: 176 202 868 176
Fragments arranged by size:
868 202 176 176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 855 887 1422
Size: 855 32 535
Fragments arranged by size:
855 535 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 195 1422
Size: 195 1227
XmaI C'CCGG_G
Cuts at: 0 249 1422
Size: 249 1173
ZraI GAC'GTC
Cuts at: 0 1122 1422
Size: 1122 300
Enzymes that do cut:
AarI AatII AclI AcuI AfeI AflII AflIII AhdI
AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI
BbvCI BfrBI BglI Bme1580I BmrI BplI BpmI Bpu10I
BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrFI BssSI BstXI BstYI Bsu36I BtgI
DraIII EaeI EagI EarI Eco57MI EcoO109I FalI HaeII
Hin4I HincII KasI MscI MspA1I NaeI NarI NcoI
NgoMIV NotI NsiI PflMI PfoI PpuMI PspOMI SacII
SapI SfcI SfiI SfoI SgrAI SmaI SmlI StuI
StyI TaqII TstI XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AgeI AleI AlfI AloI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BglII BlpI BmgBI BmtI BpuEI BsaI BsaBI BsaWI
BsaXI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI
CspCI DraI DrdI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
MmeI MslI NdeI NheI NruI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SbfI
ScaI SexAI SgfI SnaBI SpeI SphI SrfI SspI
SwaI TatI Tth111I XbaI XhoI XmnI