Restriction map for
pFN21AE4118
(Linear) (Six-base) MAPSORT of: KIEE4118.seq Check: 8884 from: 1 to: 1488
KIEE4118.orf
With 182 enzymes: SgfI *
March 22, 2012 17:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 215 963 1488
Size: 215 748 525
Fragments arranged by size:
748 525 215
AatII G_ACGT'C
Cuts at: 0 11 1488
Size: 11 1477
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1486 1488
Size: 1486 2
AfeI AGC'GCT
Cuts at: 0 140 1488
Size: 140 1348
AleI CACnn'nnGTG
Cuts at: 0 74 305 670 1488
Size: 74 231 365 818
Fragments arranged by size:
818 365 231 74
ApaI G_GGCC'C
Cuts at: 0 476 520 763 812 889 1063 1488
Size: 476 44 243 49 77 174 425
Fragments arranged by size:
476 425 243 174 77 49 44
ApaLI G'TGCA_C
Cuts at: 0 1303 1488
Size: 1303 185
AscI GG'CGCG_CC
Cuts at: 0 767 1488
Size: 767 721
AvaI C'yCGr_G
Cuts at: 0 520 762 811 913 928 1056 1178 1488
Size: 520 242 49 102 15 128 122 310
Fragments arranged by size:
520 310 242 128 122 102 49 15
BanI G'GyrC_C
Cuts at: 0 116 607 829 945 1393 1460 1488
Size: 116 491 222 116 448 67 28
Fragments arranged by size:
491 448 222 116 116 67 28
BanII G_rGCy'C
Cuts at: 0 341 476 496 520 763 812 889 974
Size: 341 135 20 24 243 49 77 85
Cuts at: 974 1063 1095 1488
Size: 89 32 393
Fragments arranged by size:
393 341 243 135 89 85 77 49
32 24 20
BbeI G_GCGC'C
Cuts at: 0 120 611 949 1488
Size: 120 491 338 539
Fragments arranged by size:
539 491 338 120
BbsI GAAGACnn'nnnn_
Cuts at: 0 986 1488
Size: 986 502
BglI GCCn_nnn'nGGC
Cuts at: 0 655 1488
Size: 655 833
BglII A'GATC_T
Cuts at: 0 1235 1488
Size: 1235 253
BlpI GC'TnA_GC
Cuts at: 0 1444 1488
Size: 1444 44
Bme1580I G_kGCm'C
Cuts at: 0 476 520 691 763 812 889 908 1063
Size: 476 44 171 72 49 77 19 155
Cuts at: 1063 1307 1488
Size: 244 181
Fragments arranged by size:
476 244 181 171 155 77 72 49
44 19
BmgBI CAC'GTC
Cuts at: 0 672 1488
Size: 672 816
BmrI ACTGGGnnnn_n'
Cuts at: 0 663 1488
Size: 663 825
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1071 1488
Size: 1071 417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 342 748 1066 1306 1354 1440 1488
Size: 342 406 318 240 48 86 48
Fragments arranged by size:
406 342 318 240 86 48 48
Bpu10I CC'TnA_GC
Cuts at: 0 495 1488
Size: 495 993
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 361 459 1354 1488
Size: 127 234 98 895 134
Fragments arranged by size:
895 234 134 127 98
BsaI GGTCTCn'nnnn_
Cuts at: 0 1274 1488
Size: 1274 214
BsaAI yAC'GTr
Cuts at: 0 1296 1488
Size: 1296 192
BsaHI Gr'CG_yC
Cuts at: 0 8 117 608 946 1195 1488
Size: 8 109 491 338 249 293
Fragments arranged by size:
491 338 293 249 109 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 339 845 1135 1174 1488
Size: 339 506 290 39 314
Fragments arranged by size:
506 339 314 290 39
BseYI C'CCAG_C
Cuts at: 0 548 774 1488
Size: 548 226 714
Fragments arranged by size:
714 548 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 268 935 1372 1456 1488
Size: 11 257 667 437 84 32
Fragments arranged by size:
667 437 257 84 32 11
BsiEI CG_ry'CG
Cuts at: 0 578 636 965 1025 1172 1224 1488
Size: 578 58 329 60 147 52 264
Fragments arranged by size:
578 329 264 147 60 58 52
BsiHKAI G_wGCw'C
Cuts at: 0 341 1127 1307 1488
Size: 341 786 180 181
Fragments arranged by size:
786 341 181 180
BsmBI CGTCTCn'nnnn_
Cuts at: 0 808 1488
Size: 808 680
Bsp1286I G_dGCh'C
Cuts at: 0 341 476 496 520 691 763 812 889
Size: 341 135 20 24 171 72 49 77
Cuts at: 889 908 974 1063 1095 1127 1307 1488
Size: 19 66 89 32 32 180 181
Fragments arranged by size:
341 181 180 171 135 89 77 72
66 49 32 32 24 20 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 119 215 761 963 1488
Size: 119 96 546 202 525
Fragments arranged by size:
546 525 202 119 96
BsrBI CCG'CTC
Cuts at: 0 104 228 732 1271 1290 1401 1488
Size: 104 124 504 539 19 111 87
Fragments arranged by size:
539 504 124 111 104 87 19
BsrDI GCAATG_nn'
Cuts at: 0 471 1488
Size: 471 1017
BsrFI r'CCGG_y
Cuts at: 0 274 502 1127 1191 1379 1488
Size: 274 228 625 64 188 109
Fragments arranged by size:
625 274 228 188 109 64
BssHII G'CGCG_C
Cuts at: 0 614 767 1488
Size: 614 153 721
Fragments arranged by size:
721 614 153
BssSI C'ACGA_G
Cuts at: 0 1357 1488
Size: 1357 131
BstXI CCAn_nnnn'nTGG
Cuts at: 0 391 1044 1488
Size: 391 653 444
Fragments arranged by size:
653 444 391
BstYI r'GATC_y
Cuts at: 0 1235 1488
Size: 1235 253
BtgI C'CryG_G
Cuts at: 0 390 578 630 791 890 1025 1140 1224
Size: 390 188 52 161 99 135 115 84
Cuts at: 1224 1488
Size: 264
Fragments arranged by size:
390 264 188 161 135 115 99 84
52
BtsI GCAGTG_nn'
Cuts at: 0 69 81 1083 1488
Size: 69 12 1002 405
Fragments arranged by size:
1002 405 69 12
EaeI y'GGCC_r
Cuts at: 0 575 633 962 1022 1034 1064 1080 1109
Size: 575 58 329 60 12 30 16 29
Cuts at: 1109 1169 1221 1488
Size: 60 52 267
Fragments arranged by size:
575 329 267 60 60 58 52 30
29 16 12
EagI C'GGCC_G
Cuts at: 0 575 633 962 1022 1169 1221 1488
Size: 575 58 329 60 147 52 267
Fragments arranged by size:
575 329 267 147 60 58 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 524 1342 1488
Size: 524 818 146
Fragments arranged by size:
818 524 146
EcoICRI GAG'CTC
Cuts at: 0 339 1488
Size: 339 1149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 342 748 1066 1306 1354 1440 1486 1488
Size: 342 406 318 240 48 86 46 2
Fragments arranged by size:
406 342 318 240 86 48 46 2
EcoO109I rG'GnC_Cy
Cuts at: 0 60 472 473 516 517 759 808 886
Size: 60 412 1 43 1 242 49 78
Cuts at: 886 1007 1488
Size: 121 481
Fragments arranged by size:
481 412 242 121 78 60 49 43
1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 204 1254 1488
Size: 204 1050 234
Fragments arranged by size:
1050 234 204
HaeII r_GCGC'y
Cuts at: 0 120 142 384 611 624 949 1108 1377
Size: 120 22 242 227 13 325 159 269
Cuts at: 1377 1488
Size: 111
Fragments arranged by size:
325 269 242 227 159 120 111 22
13
KasI G'GCGC_C
Cuts at: 0 116 607 945 1488
Size: 116 491 338 543
Fragments arranged by size:
543 491 338 116
MscI TGG'CCA
Cuts at: 0 1036 1488
Size: 1036 452
MslI CAynn'nnrTG
Cuts at: 0 74 305 600 670 1488
Size: 74 231 295 70 818
Fragments arranged by size:
818 295 231 74 70
MspA1I CmG'CkG
Cuts at: 0 46 239 548 571 580 793 835 892
Size: 46 193 309 23 9 213 42 57
Cuts at: 892 1027 1226 1488
Size: 135 199 262
Fragments arranged by size:
309 262 213 199 193 135 57 46
42 23 9
NaeI GCC'GGC
Cuts at: 0 504 1488
Size: 504 984
NarI GG'CG_CC
Cuts at: 0 117 608 946 1488
Size: 117 491 338 542
Fragments arranged by size:
542 491 338 117
NgoMIV G'CCGG_C
Cuts at: 0 502 1488
Size: 502 986
NotI GC'GGCC_GC
Cuts at: 0 575 962 1169 1488
Size: 575 387 207 319
Fragments arranged by size:
575 387 319 207
NspI r_CATG'y
Cuts at: 0 158 1488
Size: 158 1330
PasI CC'CwG_GG
Cuts at: 0 56 1488
Size: 56 1432
PmlI CAC'GTG
Cuts at: 0 1296 1488
Size: 1296 192
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1249 1281 1306 1338 1488
Size: 1249 32 25 32 150
Fragments arranged by size:
1249 150 32 32 25
PpuMI rG'GwC_Cy
Cuts at: 0 60 1007 1488
Size: 60 947 481
Fragments arranged by size:
947 481 60
PspOMI G'GGCC_C
Cuts at: 0 472 516 759 808 885 1059 1488
Size: 472 44 243 49 77 174 429
Fragments arranged by size:
472 429 243 174 77 49 44
PstI C_TGCA'G
Cuts at: 0 76 416 758 1488
Size: 76 340 342 730
Fragments arranged by size:
730 342 340 76
PvuII CAG'CTG
Cuts at: 0 548 571 1488
Size: 548 23 917
Fragments arranged by size:
917 548 23
RsrII CG'GwC_CG
Cuts at: 0 867 1488
Size: 867 621
SacI G_AGCT'C
Cuts at: 0 341 1488
Size: 341 1147
SacII CC_GC'GG
Cuts at: 0 581 794 893 1028 1227 1488
Size: 581 213 99 135 199 261
Fragments arranged by size:
581 261 213 199 135 99
SanDI GG'GwC_CC
Cuts at: 0 60 1488
Size: 60 1428
SbfI CC_TGCA'GG
Cuts at: 0 758 1488
Size: 758 730
SexAI A'CCwGG_T
Cuts at: 0 290 1488
Size: 290 1198
SfcI C'TryA_G
Cuts at: 0 72 412 754 1488
Size: 72 340 342 734
Fragments arranged by size:
734 342 340 72
SfoI GGC'GCC
Cuts at: 0 118 609 947 1488
Size: 118 491 338 541
Fragments arranged by size:
541 491 338 118
SgrAI Cr'CCGG_yG
Cuts at: 0 1127 1488
Size: 1127 361
SmaI CCC'GGG
Cuts at: 0 764 1180 1488
Size: 764 416 308
Fragments arranged by size:
764 416 308
SmlI C'TyrA_G
Cuts at: 0 142 376 438 1369 1488
Size: 142 234 62 931 119
Fragments arranged by size:
931 234 142 119 62
SrfI GCCC'GGGC
Cuts at: 0 764 1488
Size: 764 724
StyI C'CwwG_G
Cuts at: 0 254 1115 1488
Size: 254 861 373
Fragments arranged by size:
861 373 254
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 782 1488
Size: 782 706
XmaI C'CCGG_G
Cuts at: 0 762 1178 1488
Size: 762 416 310
Fragments arranged by size:
762 416 310
XmnI GAAnn'nnTTC
Cuts at: 0 679 1237 1264 1321 1488
Size: 679 558 27 57 167
Fragments arranged by size:
679 558 167 57 27
ZraI GAC'GTC
Cuts at: 0 9 1488
Size: 9 1479
Enzymes that do cut:
AarI AatII AcuI AfeI AleI ApaI ApaLI AscI
AvaI BanI BanII BbeI BbsI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstXI
BstYI BtgI BtsI EaeI EagI EciI EcoICRI Eco57MI
EcoO109I FalI HaeII KasI MscI MslI MspA1I NaeI
NarI NgoMIV NotI NspI PasI PmlI PpiI PpuMI
PspOMI PstI PvuII RsrII SacI SacII SanDI SbfI
SexAI SfcI SfoI SgrAI SmaI SmlI SrfI StyI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AhdI AlfI
AloI AlwNI ApoI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BmtI BsaBI BsaWI
BsaXI BsiWI BsmI BspEI BspHI BsrGI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EarI EcoNI EcoRI EcoRV FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
NcoI NdeI NheI NruI NsiI PacI PciI PflMI
PfoI PmeI PshAI PsiI PspXI PsrI PvuI SalI
SapI ScaI SfiI SgfI SnaBI SpeI SphI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI