Restriction map for
pFN21AE4117
(Linear) (Six-base) MAPSORT of: KIEE4117.seq Check: 2407 from: 1 to: 1482
KIEE4117.orf
With 182 enzymes: SgfI *
March 22, 2012 17:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 748 841 1251 1469 1482
Size: 559 189 93 410 218 13
Fragments arranged by size:
559 410 218 189 93 13
AflII C'TTAA_G
Cuts at: 0 253 1482
Size: 253 1229
AflIII A'CryG_T
Cuts at: 0 1134 1482
Size: 1134 348
AlwNI CAG_nnn'CTG
Cuts at: 0 1345 1482
Size: 1345 137
ApoI r'AATT_y
Cuts at: 0 508 1482
Size: 508 974
BamHI G'GATC_C
Cuts at: 0 970 1482
Size: 970 512
BanI G'GyrC_C
Cuts at: 0 30 1482
Size: 30 1452
BanII G_rGCy'C
Cuts at: 0 1254 1482
Size: 1254 228
BlpI GC'TnA_GC
Cuts at: 0 230 1482
Size: 230 1252
BmgBI CAC'GTC
Cuts at: 0 822 1482
Size: 822 660
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 1322 1394 1482
Size: 667 655 72 88
Fragments arranged by size:
667 655 88 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1482
Size: 295 1187
BsaI GGTCTCn'nnnn_
Cuts at: 0 302 1084 1482
Size: 302 782 398
Fragments arranged by size:
782 398 302
BsaWI w'CCGG_w
Cuts at: 0 293 1482
Size: 293 1189
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 698 1102 1482
Size: 698 404 380
Fragments arranged by size:
698 404 380
BseYI C'CCAG_C
Cuts at: 0 276 1094 1482
Size: 276 818 388
Fragments arranged by size:
818 388 276
BsiEI CG_ry'CG
Cuts at: 0 51 1482
Size: 51 1431
Bsp1286I G_dGCh'C
Cuts at: 0 1254 1482
Size: 1254 228
BspHI T'CATG_A
Cuts at: 0 1140 1482
Size: 1140 342
BsrDI GCAATG_nn'
Cuts at: 0 90 362 1482
Size: 90 272 1120
Fragments arranged by size:
1120 272 90
BsrFI r'CCGG_y
Cuts at: 0 665 1482
Size: 665 817
BstYI r'GATC_y
Cuts at: 0 970 1482
Size: 970 512
BtsI GCAGTG_nn'
Cuts at: 0 842 1482
Size: 842 640
ClaI AT'CG_AT
Cuts at: 0 206 1482
Size: 206 1276
DraIII CAC_nnn'GTG
Cuts at: 0 19 1482
Size: 19 1463
EaeI y'GGCC_r
Cuts at: 0 48 829 1349 1482
Size: 48 781 520 133
Fragments arranged by size:
781 520 133 48
EagI C'GGCC_G
Cuts at: 0 48 1482
Size: 48 1434
EarI CTCTTCn'nnn_
Cuts at: 0 697 1103 1482
Size: 697 406 379
Fragments arranged by size:
697 406 379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 667 748 841 1251 1322 1394 1469
Size: 559 108 81 93 410 71 72 75
Cuts at: 1469 1482
Size: 13
Fragments arranged by size:
559 410 108 93 81 75 72 71
13
EcoO109I rG'GnC_Cy
Cuts at: 0 341 1290 1482
Size: 341 949 192
Fragments arranged by size:
949 341 192
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 268 1482
Size: 268 1214
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 375 407 484 516 1482
Size: 375 32 77 32 966
Fragments arranged by size:
966 375 77 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 471 1482
Size: 171 300 1011
Fragments arranged by size:
1011 300 171
MscI TGG'CCA
Cuts at: 0 831 1351 1482
Size: 831 520 131
Fragments arranged by size:
831 520 131
MslI CAynn'nnrTG
Cuts at: 0 518 590 1139 1482
Size: 518 72 549 343
Fragments arranged by size:
549 518 343 72
MspA1I CmG'CkG
Cuts at: 0 234 1038 1414 1482
Size: 234 804 376 68
Fragments arranged by size:
804 376 234 68
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1239 1271 1482
Size: 1239 32 211
Fragments arranged by size:
1239 211 32
PpuMI rG'GwC_Cy
Cuts at: 0 341 1290 1482
Size: 341 949 192
Fragments arranged by size:
949 341 192
PstI C_TGCA'G
Cuts at: 0 70 1482
Size: 70 1412
PvuII CAG'CTG
Cuts at: 0 234 1038 1414 1482
Size: 234 804 376 68
Fragments arranged by size:
804 376 234 68
SanDI GG'GwC_CC
Cuts at: 0 341 1482
Size: 341 1141
SapI GCTCTTCn'nnn_
Cuts at: 0 697 1482
Size: 697 785
SfcI C'TryA_G
Cuts at: 0 66 1482
Size: 66 1416
SmlI C'TyrA_G
Cuts at: 0 253 310 1482
Size: 253 57 1172
Fragments arranged by size:
1172 253 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 806 838 1482
Size: 806 32 644
Fragments arranged by size:
806 644 32
Enzymes that do cut:
AcuI AflII AflIII AlwNI ApoI BamHI BanI BanII
BlpI BmgBI BpmI BpuEI BsaI BsaWI BseRI BseYI
BsiEI Bsp1286I BspHI BsrDI BsrFI BstYI BtsI ClaI
DraIII EaeI EagI EarI Eco57MI EcoO109I FalI Hin4I
MmeI MscI MslI MspA1I PpiI PpuMI PstI PvuII
SanDI SapI SfcI SmlI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII Bme1580I BmrI BmtI BplI
Bpu10I BsaAI BsaBI BsaHI BsaXI BsgI BsiHKAI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
CspCI DraI DrdI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII TatI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI