Restriction map for
pFN21AE4112
(Linear) (Six-base) MAPSORT of: KIEE4112.seq Check: 4361 from: 1 to: 1449
KIEE4112.orf
With 182 enzymes: SgfI *
March 22, 2012 17:23 ..
AccI GT'mk_AC
Cuts at: 0 100 174 563 870 1268 1449
Size: 100 74 389 307 398 181
Fragments arranged by size:
398 389 307 181 100 74
AflII C'TTAA_G
Cuts at: 0 1114 1449
Size: 1114 335
AlwNI CAG_nnn'CTG
Cuts at: 0 1315 1449
Size: 1315 134
ApoI r'AATT_y
Cuts at: 0 76 880 1449
Size: 76 804 569
Fragments arranged by size:
804 569 76
BanII G_rGCy'C
Cuts at: 0 504 1449
Size: 504 945
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1411 1445 1449
Size: 1411 34 4
Fragments arranged by size:
1411 34 4
BfrBI ATG'CAT
Cuts at: 0 843 1353 1449
Size: 843 510 96
Fragments arranged by size:
843 510 96
BglII A'GATC_T
Cuts at: 0 825 1449
Size: 825 624
Bme1580I G_kGCm'C
Cuts at: 0 837 927 1449
Size: 837 90 522
Fragments arranged by size:
837 522 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1199 1449
Size: 1199 250
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 457 487 1449
Size: 457 30 962
Fragments arranged by size:
962 457 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 164 1449
Size: 164 1285
BseYI C'CCAG_C
Cuts at: 0 403 460 1449
Size: 403 57 989
Fragments arranged by size:
989 403 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 566 1449
Size: 566 883
BsiHKAI G_wGCw'C
Cuts at: 0 504 867 1449
Size: 504 363 582
Fragments arranged by size:
582 504 363
Bsp1286I G_dGCh'C
Cuts at: 0 504 837 867 927 1449
Size: 504 333 30 60 522
Fragments arranged by size:
522 504 333 60 30
BspHI T'CATG_A
Cuts at: 0 249 1449
Size: 249 1200
BssSI C'ACGA_G
Cuts at: 0 236 1449
Size: 236 1213
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1315 1449
Size: 1315 134
BstBI TT'CG_AA
Cuts at: 0 1227 1449
Size: 1227 222
BstYI r'GATC_y
Cuts at: 0 616 825 1449
Size: 616 209 624
Fragments arranged by size:
624 616 209
BtsI GCAGTG_nn'
Cuts at: 0 405 1449
Size: 405 1044
EaeI y'GGCC_r
Cuts at: 0 621 1085 1449
Size: 621 464 364
Fragments arranged by size:
621 464 364
EcoICRI GAG'CTC
Cuts at: 0 502 1449
Size: 502 947
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1199 1449
Size: 1199 250
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 736 768 891 923 1449
Size: 736 32 123 32 526
Fragments arranged by size:
736 526 123 32 32
HincII GTy'rAC
Cuts at: 0 156 1449
Size: 156 1293
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 1381 1449
Size: 325 1056 68
Fragments arranged by size:
1056 325 68
MscI TGG'CCA
Cuts at: 0 1087 1449
Size: 1087 362
MslI CAynn'nnrTG
Cuts at: 0 166 1449
Size: 166 1283
MspA1I CmG'CkG
Cuts at: 0 460 1315 1341 1449
Size: 460 855 26 108
Fragments arranged by size:
855 460 108 26
NsiI A_TGCA'T
Cuts at: 0 845 1355 1449
Size: 845 510 94
Fragments arranged by size:
845 510 94
NspI r_CATG'y
Cuts at: 0 779 1267 1449
Size: 779 488 182
Fragments arranged by size:
779 488 182
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 249 281 1449
Size: 249 32 1168
Fragments arranged by size:
1168 249 32
PshAI GACnn'nnGTC
Cuts at: 0 219 1449
Size: 219 1230
PstI C_TGCA'G
Cuts at: 0 1320 1449
Size: 1320 129
PvuII CAG'CTG
Cuts at: 0 460 1315 1341 1449
Size: 460 855 26 108
Fragments arranged by size:
855 460 108 26
SacI G_AGCT'C
Cuts at: 0 504 1449
Size: 504 945
ScaI AGT'ACT
Cuts at: 0 126 1449
Size: 126 1323
SfcI C'TryA_G
Cuts at: 0 1316 1449
Size: 1316 133
SmlI C'TyrA_G
Cuts at: 0 1114 1449
Size: 1114 335
SspI AAT'ATT
Cuts at: 0 1021 1449
Size: 1021 428
StuI AGG'CCT
Cuts at: 0 1176 1449
Size: 1176 273
StyI C'CwwG_G
Cuts at: 0 5 104 1449
Size: 5 99 1345
Fragments arranged by size:
1345 99 5
TatI w'GTAC_w
Cuts at: 0 124 1449
Size: 124 1325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1402 1449
Size: 1402 47
XmnI GAAnn'nnTTC
Cuts at: 0 126 684 1449
Size: 126 558 765
Fragments arranged by size:
765 558 126
Enzymes that do cut:
AccI AflII AlwNI ApoI BanII BcgI BfrBI BglII
Bme1580I BpmI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BssSI BstAPI BstBI BstYI BtsI EaeI EcoICRI
Eco57MI Hin4I HincII MmeI MscI MslI MspA1I NsiI
NspI PpiI PshAI PstI PvuII SacI ScaI SfcI
SmlI SspI StuI StyI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BciVI BclI BglI BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PflMI PfoI PmeI PmlI PpuMI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI ZraI