Restriction map for
pFN21AE4110
(Linear) (Six-base) MAPSORT of: KIEE4110.seq Check: 7829 from: 1 to: 1443
KIEE4110.orf
With 182 enzymes: SgfI *
March 22, 2012 17:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1278 1443
Size: 1278 165
Acc65I G'GTAC_C
Cuts at: 0 61 1443
Size: 61 1382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1291 1443
Size: 1291 152
AflIII A'CryG_T
Cuts at: 0 1094 1443
Size: 1094 349
AgeI A'CCGG_T
Cuts at: 0 804 1443
Size: 804 639
AleI CACnn'nnGTG
Cuts at: 0 1099 1443
Size: 1099 344
ApoI r'AATT_y
Cuts at: 0 658 1443
Size: 658 785
AvaI C'yCGr_G
Cuts at: 0 1346 1443
Size: 1346 97
BanI G'GyrC_C
Cuts at: 0 61 1443
Size: 61 1382
BanII G_rGCy'C
Cuts at: 0 749 996 1249 1355 1409 1441 1443
Size: 749 247 253 106 54 32 2
Fragments arranged by size:
749 253 247 106 54 32 2
BglI GCCn_nnn'nGGC
Cuts at: 0 216 1443
Size: 216 1227
BglII A'GATC_T
Cuts at: 0 314 683 1443
Size: 314 369 760
Fragments arranged by size:
760 369 314
BmrI ACTGGGnnnn_n'
Cuts at: 0 280 1443
Size: 280 1163
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1316 1443
Size: 1316 127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 238 424 736 1443
Size: 172 66 186 312 707
Fragments arranged by size:
707 312 186 172 66
Bpu10I CC'TnA_GC
Cuts at: 0 1435 1443
Size: 1435 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 663 1443
Size: 663 780
BsaAI yAC'GTr
Cuts at: 0 1097 1443
Size: 1097 346
BsaWI w'CCGG_w
Cuts at: 0 804 1443
Size: 804 639
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1189 1219 1443
Size: 1189 30 224
Fragments arranged by size:
1189 224 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 298 487 733 958 1210 1443
Size: 169 129 189 246 225 252 233
Fragments arranged by size:
252 246 233 225 189 169 129
BseYI C'CCAG_C
Cuts at: 0 370 676 825 851 1034 1319 1443
Size: 370 306 149 26 183 285 124
Fragments arranged by size:
370 306 285 183 149 124 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1119 1183 1443
Size: 1119 64 260
Fragments arranged by size:
1119 260 64
BsiHKAI G_wGCw'C
Cuts at: 0 305 380 702 749 996 1441 1443
Size: 305 75 322 47 247 445 2
Fragments arranged by size:
445 322 305 247 75 47 2
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1239 1443
Size: 1239 204
Bsp1286I G_dGCh'C
Cuts at: 0 305 380 702 749 996 1249 1355 1409
Size: 305 75 322 47 247 253 106 54
Cuts at: 1409 1441 1443
Size: 32 2
Fragments arranged by size:
322 305 253 247 106 75 54 47
32 2
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1278 1443
Size: 1278 165
BsrFI r'CCGG_y
Cuts at: 0 75 689 804 1443
Size: 75 614 115 639
Fragments arranged by size:
639 614 115 75
BstEII G'GTnAC_C
Cuts at: 0 1323 1443
Size: 1323 120
BstYI r'GATC_y
Cuts at: 0 314 683 1443
Size: 314 369 760
Fragments arranged by size:
760 369 314
Bsu36I CC'TnA_GG
Cuts at: 0 67 574 1443
Size: 67 507 869
Fragments arranged by size:
869 507 67
BtgI C'CryG_G
Cuts at: 0 1122 1139 1443
Size: 1122 17 304
Fragments arranged by size:
1122 304 17
BtsI GCAGTG_nn'
Cuts at: 0 1367 1443
Size: 1367 76
DrdI GACnn_nn'nnGTC
Cuts at: 0 531 1443
Size: 531 912
EaeI y'GGCC_r
Cuts at: 0 93 1189 1443
Size: 93 1096 254
Fragments arranged by size:
1096 254 93
EcoICRI GAG'CTC
Cuts at: 0 747 994 1439 1443
Size: 747 247 445 4
Fragments arranged by size:
747 445 247 4
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 238 424 736 1291 1443
Size: 172 66 186 312 555 152
Fragments arranged by size:
555 312 186 172 152 66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 526 1380 1443
Size: 526 854 63
Fragments arranged by size:
854 526 63
EcoO109I rG'GnC_Cy
Cuts at: 0 858 1443
Size: 858 585
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 336 987 1443
Size: 336 651 456
Fragments arranged by size:
651 456 336
HaeII r_GCGC'y
Cuts at: 0 252 1443
Size: 252 1191
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 299 331 1340 1372 1443
Size: 299 32 1009 32 71
Fragments arranged by size:
1009 299 71 32 32
HincII GTy'rAC
Cuts at: 0 42 1443
Size: 42 1401
KpnI G_GTAC'C
Cuts at: 0 65 1443
Size: 65 1378
MfeI C'AATT_G
Cuts at: 0 616 1443
Size: 616 827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 893 1443
Size: 893 550
MslI CAynn'nnrTG
Cuts at: 0 1099 1443
Size: 1099 344
MspA1I CmG'CkG
Cuts at: 0 222 258 415 867 1133 1167 1443
Size: 222 36 157 452 266 34 276
Fragments arranged by size:
452 276 266 222 157 36 34
NcoI C'CATG_G
Cuts at: 0 1122 1443
Size: 1122 321
PfoI T'CCnGG_A
Cuts at: 0 401 1443
Size: 401 1042
PmlI CAC'GTG
Cuts at: 0 1097 1443
Size: 1097 346
PstI C_TGCA'G
Cuts at: 0 872 1443
Size: 872 571
PvuII CAG'CTG
Cuts at: 0 222 258 1133 1443
Size: 222 36 875 310
Fragments arranged by size:
875 310 222 36
SacI G_AGCT'C
Cuts at: 0 749 996 1441 1443
Size: 749 247 445 2
Fragments arranged by size:
749 445 247 2
SfcI C'TryA_G
Cuts at: 0 423 868 1443
Size: 423 445 575
Fragments arranged by size:
575 445 423
SmlI C'TyrA_G
Cuts at: 0 642 1443
Size: 642 801
StyI C'CwwG_G
Cuts at: 0 1122 1443
Size: 1122 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1089 1311 1443
Size: 1089 222 132
Fragments arranged by size:
1089 222 132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1189 1221 1443
Size: 1189 32 222
Fragments arranged by size:
1189 222 32
XbaI T'CTAG_A
Cuts at: 0 585 939 1443
Size: 585 354 504
Fragments arranged by size:
585 504 354
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 219 273 399 1059 1443
Size: 219 54 126 660 384
Fragments arranged by size:
660 384 219 126 54
Enzymes that do cut:
AarI Acc65I AcuI AflIII AgeI AleI ApoI AvaI
BanI BanII BglI BglII BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspMI BsrFI BstEII BstYI Bsu36I BtgI
BtsI DrdI EaeI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII KpnI MfeI MmeI MslI MspA1I
NcoI PfoI PmlI PstI PvuII SacI SfcI SmlI
StyI TaqII TstI XbaI XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AhdI AlfI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BlpI Bme1580I BmgBI BmtI BsaI BsaBI BsaHI BsiEI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I BtgZI ClaI CspCI
DraI DraIII EagI EarI EciI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KasI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI Tth111I XhoI XmaI XmnI ZraI